BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,749,192	A→G	V186A (GTG→GCG)	b1672 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,749,192	0	A	G	99.1%	314.4 / ‑4.7	105	V186A (GTG→GCG)	b1672	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (61/43); total (62/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.97e-01

Only 100 of 123 total aligned reads displayed.

GGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGG  >  NC_000913/1749104‑1749281

ggggTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAAcgccgc                                                                                       <  2:894874/93‑1 (MQ=255)

ggggTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAAcgccgc                                                                                       <  1:1108062/93‑1 (MQ=255)

 gggTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACgccgcc                                                                                      >  1:2769039/1‑93 (MQ=255)

   gTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGt                                                                                    >  2:4590119/1‑93 (MQ=255)

    ttCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTc                                                                                   >  2:3353342/1‑93 (MQ=255)

       tGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGAt                                                                                <  1:1372008/93‑1 (MQ=255)

        gAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATg                                                                               <  2:3748610/93‑1 (MQ=255)

         aaaCTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGa                                                                              >  1:1631756/1‑93 (MQ=255)

         aaaCTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGa                                                                              <  1:4169627/93‑1 (MQ=255)

            cTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTc                                                                           >  1:464311/1‑93 (MQ=255)

              gCTCTACCTCGACCCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCa                                                                         <  2:735596/93‑1 (MQ=255)

              gCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCa                                                                         >  1:837111/1‑93 (MQ=255)

               ctctACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCAc                                                                        >  2:3388372/1‑93 (MQ=255)

                tctACCTCGACCCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATTACTCCAcc                                                                       <  2:1481870/93‑1 (MQ=255)

                tctACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCAcc                                                                       >  1:2697622/1‑93 (MQ=255)

                 ctACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCt                                                                      <  2:244197/93‑1 (MQ=255)

                   aCCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGc                                                                    <  1:4154354/93‑1 (MQ=255)

                      tCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGt                                                                 >  2:2937129/1‑93 (MQ=255)

                      tCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGt                                                                 >  1:4592289/1‑93 (MQ=255)

                      tCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGt                                                                 >  1:2402625/1‑93 (MQ=255)

                        gAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGtat                                                               >  1:1317376/1‑93 (MQ=255)

                         aaCCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGtata                                                              <  2:4486994/93‑1 (MQ=255)

                         aaCCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGtata                                                              <  2:2691092/93‑1 (MQ=255)

                          aCCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTatat                                                             <  1:2892241/93‑1 (MQ=255)

                           ccATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATc                                                            >  1:1105995/1‑93 (MQ=255)

                           ccATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATc                                                            <  2:2796856/93‑1 (MQ=255)

                           ccATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATc                                                            <  1:1554241/93‑1 (MQ=255)

                            cATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCg                                                           >  1:941562/1‑93 (MQ=255)

                            cATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCg                                                           >  2:416692/1‑93 (MQ=255)

                             aTTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCgg                                                          >  1:1300312/1‑93 (MQ=255)

                              ttCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGc                                                         >  1:2968294/1‑93 (MQ=255)

                              ttCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGc                                                         <  1:4096938/93‑1 (MQ=255)

                                cGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCat                                                       >  2:3437122/1‑93 (MQ=255)

                                 gCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCata                                                      >  1:3274061/1‑93 (MQ=255)

                                  cataagccataagcCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCatat                                                     >  2:2187935/1‑93 (MQ=255)

                                  cataagccataagcCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCatat                                                     >  1:3553801/1‑93 (MQ=255)

                                    taagccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTc                                                   >  2:1635390/1‑93 (MQ=255)

                                    taagccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTc                                                   >  2:1014386/1‑93 (MQ=255)

                                    taagccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTc                                                   >  1:4519681/1‑93 (MQ=255)

                                     aagccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCa                                                  <  1:3462459/93‑1 (MQ=255)

                                     aagccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCa                                                  <  2:1560506/93‑1 (MQ=255)

                                     aagccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCa                                                  <  2:1207242/93‑1 (MQ=255)

                                      agccaaaagccTTGCCAAAGGTCAAAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAg                                                 <  2:1219118/93‑1 (MQ=255)

                                       gccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAgg                                                <  1:1772611/93‑1 (MQ=255)

                                        ccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGt                                               <  2:4358906/93‑1 (MQ=255)

                                         cataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTg                                              >  1:3869113/1‑93 (MQ=255)

                                         cataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTg                                              >  2:3268335/1‑93 (MQ=255)

                                          ataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGc                                             >  1:3541674/1‑93 (MQ=255)

                                          ataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGc                                             >  1:477346/1‑93 (MQ=255)

                                           taagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCa                                            >  2:787695/1‑93 (MQ=255)

                                            aagccTTGCCATAGGTTATAAATCGGCCCGTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCaa                                           <  1:182217/93‑1 (MQ=255)

                                            aagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCaa                                           >  1:4151828/1‑93 (MQ=255)

                                             agccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAAc                                          >  1:1452423/1‑93 (MQ=255)

                                              gccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAAca                                         >  1:1565091/1‑93 (MQ=255)

                                              gccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAAca                                         <  2:4244254/93‑1 (MQ=255)

                                               ccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCCCCTGCCGTATATCGGCATATTCAGGTGCAAcat                                        <  1:1448879/93‑1 (MQ=255)

                                                cTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAAcatc                                       >  1:3958990/1‑93 (MQ=255)

                                                 ttGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAAcatcc                                      <  1:4683607/93‑1 (MQ=255)

                                                 ttGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAAcatcc                                      >  2:4188654/1‑93 (MQ=255)

                                                 ttGCCATAGGTCATAAATCGCTAGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAAcatcc                                      >  2:3813108/1‑93 (MQ=255)

                                                  tGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAAcatcca                                     <  1:1148765/93‑1 (MQ=255)

                                                  tGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAAcatcca                                     <  1:3352259/93‑1 (MQ=255)

                                                   gCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCAATGACTCCACCTGCCGTATATCTGCATATTCAGGTGCAAcatccat                                    <  2:3781054/93‑1 (MQ=255)

                                                    ctacaGGCCATAATTAGATCGCTAATCAACGACTATGCCACAGTCGATGACTCCGCCTGCCGTATATCGGCATATTCAGGTGCAAcatccatc                                   <  2:2970764/89‑1 (MQ=255)

                                                    ccATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAAcatccatc                                   <  2:950068/93‑1 (MQ=255)

                                                     cATAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATcg                                  >  1:1037863/1‑93 (MQ=255)

                                                      aTAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCTTATATCGGCATATTCAGGTGCAACATCCATcgc                                 <  2:3857121/93‑1 (MQ=255)

                                                      aTAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATcgc                                 >  1:3575482/1‑93 (MQ=255)

                                                      aTAGGTCATAAATCGCTCGCTAAACAAGTACAACGACGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATcgc                                 >  1:3566819/1‑93 (MQ=255)

                                                       tAGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATcgcg                                >  2:3976107/1‑93 (MQ=255)

                                                        aGGTCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCgcgg                               <  2:2189977/93‑1 (MQ=255)

                                                          gTCAAAAATCGCTCGCGAAACAAGTATGACGCCGCCATCGATGCCTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCgcggcg                             <  1:2650152/93‑1 (MQ=255)

                                                           tCATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGa                            >  2:3193750/1‑93 (MQ=255)

                                                            cATAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGAt                           >  1:2075848/1‑93 (MQ=255)

                                                            cATAAATCGCTCGCTAAACAAGTACAACCCCGCCGTCGATCACTCCGCCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGAt                           <  1:3121651/93‑1 (MQ=255)

                                                            cATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGAt                           >  1:3091026/1‑93 (MQ=255)

                                                             aTAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATc                          <  2:1935875/93‑1 (MQ=255)

                                                              tAAATCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATcg                         >  2:137933/1‑93 (MQ=255)

                                                                 aTCGCTCGCTAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCgg                      >  2:2035383/1‑93 (MQ=255)

                                                                   cgctcgctAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCt                    >  2:3118870/1‑93 (MQ=255)

                                                                     ctcgctAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTc                  >  2:1729102/1‑93 (MQ=255)

                                                                         ctAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATc              <  1:3562808/93‑1 (MQ=255)

                                                                         ctAAACAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATc              <  1:3457176/93‑1 (MQ=255)

                                                                          aaaaCAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCt             <  2:18941/92‑1 (MQ=255)

                                                                            aaCAAGTACACCGCCGCCGTCGATGCCTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTgc           <  1:2795069/93‑1 (MQ=255)

                                                                            aaCAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTgc           >  1:2224962/1‑93 (MQ=255)

                                                                            aaCAAGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTgc           <  1:3147664/93‑1 (MQ=255)

                                                                            aaCAAGTACAACGCCGCCGGCGATGCCTCCGCCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTgc           <  1:1810028/93‑1 (MQ=255)

                                                                                aGTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCtt       >  1:1134318/1‑93 (MQ=255)

                                                                                 gTACAGCGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTc      <  1:2203255/93‑1 (MQ=255)

                                                                                 gTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTc      <  2:2127123/93‑1 (MQ=255)

                                                                                 gTACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTc      >  1:775069/1‑93 (MQ=255)

                                                                                  tACACCCCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTcc     <  2:921630/93‑1 (MQ=255)

                                                                                  tACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCCATCTGCGCTTcc     >  2:882928/1‑93 (MQ=255)

                                                                                  tACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTcc     >  2:253363/1‑93 (MQ=255)

                                                                                  tACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTcc     >  1:3807708/1‑93 (MQ=255)

                                                                                  tACAACGCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTcc     <  1:395724/93‑1 (MQ=255)

                                                                                   acaacgCCGCCGTCGATGCCTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCt    <  2:3368960/93‑1 (MQ=255)

                                                                                   acaacgCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCt    >  1:1351478/1‑93 (MQ=255)

                                                                                   acaacgCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGCCGATCGCGGCTTCAATCTGCGGTTCCt    >  1:2357278/1‑93 (MQ=255)

GGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGG  >  NC_000913/1749104‑1749281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`