Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,758,261	T→C	E96G (GAA→GGA)	b1680 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,758,261	0	T	C	100.0%	267.1 / NA	86	E96G (GAA→GGA)	b1680	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/49);  total (37/49)

TTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCT  >  NC_000913/1758171‑1758348                                                                                           |                                                                                         ttAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGAtccctccc                                                                                        >  2:2154230/1‑93 (MQ=255)     cGTGGTACTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCcgtcg                                                                                    <  2:3645867/93‑1 (MQ=255)      gtggtgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCcgtcgt                                                                                   <  2:495635/93‑1 (MQ=255)        ggtgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGc                                                                                 >  2:1021137/1‑93 (MQ=255)         gtgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGcc                                                                                <  2:4551683/93‑1 (MQ=255)          tgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCcg                                                                               >  1:4662659/1‑93 (MQ=255)             tCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCgg                                                                            >  2:3279873/1‑93 (MQ=255)             tCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCgg                                                                            >  2:1554644/1‑93 (MQ=255)             tCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCgg                                                                            >  1:646234/1‑93 (MQ=255)              ccATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGACCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGa                                                                           >  1:1570349/1‑93 (MQ=255)               cATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACAAGATTGATCCCTCCCGTCGTGCCGCGGAc                                                                          >  2:1402968/1‑93 (MQ=255)                 tCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑Ga                                                                       >  2:3545834/1‑93 (MQ=255)                 tCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCCTCGTGCCGCGGAC‑Ga                                                                       <  2:552719/93‑1 (MQ=255)                  ctgactgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑Gaa                                                                      <  2:3045924/93‑1 (MQ=255)                   tgactgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAac                                                                     >  2:1398824/1‑93 (MQ=255)                    gactgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAaca                                                                    <  2:954961/93‑1 (MQ=255)                     actgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAacac                                                                   >  2:2654094/1‑93 (MQ=255)                     actgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAacac                                                                   <  1:2431315/93‑1 (MQ=255)                     actgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAacac                                                                   <  1:515710/93‑1 (MQ=255)                        gatgatgatGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAg                                                                <  1:1198934/93‑1 (MQ=255)                         atgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGc                                                               <  1:4635326/93‑1 (MQ=255)                         atgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCAGTCGTGCCGCGGAC‑GAACACCAGc                                                               >  1:592224/1‑93 (MQ=255)                         atgataatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCTAGCGATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGc                                                               <  2:917046/93‑1 (MQ=255)                          tgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGTC‑GAACACCAGct                                                              >  1:1429987/1‑93 (MQ=255)                          tgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGct                                                              <  1:1562791/93‑1 (MQ=255)                           gatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGctc                                                             >  2:2384187/1‑93 (MQ=255)                           gatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGctc                                                             <  1:2900903/93‑1 (MQ=255)                            atgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGctct                                                            <  2:1413851/93‑1 (MQ=255)                            atgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGctct                                                            >  1:4054202/1‑93 (MQ=255)                              gatgTTATCGCCGGCCCGCACGTTGCTGTTGCCACAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTc                                                          <  1:2139728/93‑1 (MQ=255)                              gatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTc                                                          <  1:545846/93‑1 (MQ=255)                               atgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTcc                                                         <  2:4390640/93‑1 (MQ=255)                               atgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTcc                                                         <  1:399204/93‑1 (MQ=255)                                tgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTccg                                                        <  2:4361277/93‑1 (MQ=255)                                 gTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTccgc                                                       <  1:3176252/93‑1 (MQ=255)                                 gTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTccgc                                                       >  2:3257120/1‑93 (MQ=255)                                   tATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTccgccg                                                     <  2:3107839/93‑1 (MQ=255)                                   tATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTccgccg                                                     >  2:64205/1‑93 (MQ=255)                                     tCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGaa                                                   <  2:434035/93‑1 (MQ=255)                                       gcccgcccGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACg                                                 <  2:2792982/93‑1 (MQ=255)                                        cccgcccgCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACgg                                                >  1:4228639/1‑93 (MQ=255)                                            cccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCAt                                            >  2:1731370/1‑93 (MQ=255)                                            cccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCAt                                            >  2:955918/1‑93 (MQ=255)                                             ccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCAtt                                           >  2:1765765/1‑93 (MQ=255)                                              cgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GACCACCAGCTCTTCCGCCGAACGGGCCTta                                          >  2:1135001/1‑93 (MQ=255)                                              cgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATta                                          >  2:731094/1‑93 (MQ=255)                                              cgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATta                                          >  1:733146/1‑93 (MQ=255)                                                 ccGTTTCTGTTGCCCCAGCGATTGGCGACCAGATTGATCCCTCCCGTCGTGCTGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATtaata                                       <  2:3827221/92‑1 (MQ=255)                                                  cGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATtaataa                                      <  2:1516246/93‑1 (MQ=255)                                                  cGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATtaataa                                      <  1:2151475/93‑1 (MQ=255)                                                   gTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATaaa                                     >  2:1949352/1‑93 (MQ=255)                                                    ttGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAAc                                    <  1:141444/93‑1 (MQ=255)                                                    ttGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAAc                                    >  2:627927/1‑93 (MQ=255)                                                    ttGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAAc                                    <  1:2707821/93‑1 (MQ=255)                                                    ttGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAAc                                    <  2:4506575/93‑1 (MQ=255)                                                     tGCTGTTGCCCCAGGCATTTGCGGCCAGATTTGTTCCTCCCCTCGTGCCGCGGGCGGAAAACCA‑CTCCTTCGCCGGACCGGCATTAATaaaaa                                   >  2:2649713/1‑91 (MQ=255)                                                     tGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACa                                   <  1:714980/93‑1 (MQ=255)                                                     tGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACa                                   <  1:545823/93‑1 (MQ=255)                                                       cTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGc                                 <  2:561887/93‑1 (MQ=255)                                                       cTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGc                                 >  2:3014624/1‑93 (MQ=255)                                                         gTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGa                               <  2:435830/93‑1 (MQ=255)                                                         gTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGa                               >  2:4503309/1‑93 (MQ=255)                                                         gTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGa                               <  1:908469/93‑1 (MQ=255)                                                            gCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGc                            >  1:79088/1‑93 (MQ=255)                                                             ccccAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGcc                           <  1:961744/93‑1 (MQ=255)                                                             ccccAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGcc                           <  1:627927/93‑1 (MQ=255)                                                             ccccAGCTATTGGCCACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GACCACCAGCTCTTCCGCCGATCGGGCATTAATAAACAGCGATGcc                           <  2:4233982/93‑1 (MQ=255)                                                                cAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGc                        >  2:2064005/1‑93 (MQ=255)                                                                cAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGc                        >  2:2494225/1‑93 (MQ=255)                                                                 aGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCt                       <  2:1576029/93‑1 (MQ=255)                                                                  gCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCtt                      <  1:3390634/93‑1 (MQ=255)                                                                   cTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTg                     <  1:4070456/93‑1 (MQ=255)                                                                    tATTGGCGCCCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgc                    <  1:965685/93‑1 (MQ=255)                                                                    tATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GATCACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgc                    >  1:3308312/1‑93 (MQ=255)                                                                    tATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgc                    <  1:2534055/93‑1 (MQ=255)                                                                       tGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgcgcc                 >  2:413486/1‑92 (MQ=255)                                                                       tGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCa                 <  2:2426870/93‑1 (MQ=255)                                                                       tGGCGACCAGATTGATCCCTCCCCTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCa                 <  2:1630623/93‑1 (MQ=255)                                                                         gCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACg               <  1:1042727/93‑1 (MQ=255)                                                                            aCCAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTtc            <  1:3326003/93‑1 (MQ=255)                                                                             ccAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTtct           >  1:980318/1‑93 (MQ=255)                                                                              cAGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTtctc          >  2:753324/1‑93 (MQ=255)                                                                               aGATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTcc         >  2:2144445/1‑93 (MQ=255)                                                                                gATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCa        <  2:192649/93‑1 (MQ=255)                                                                                gATTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCa        <  2:790113/93‑1 (MQ=255)                                                                                 aTTGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAt       <  2:3171035/93‑1 (MQ=255)                                                                                  ttGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAtt      >  2:3001087/1‑93 (MQ=255)                                                                                  ttGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAtt      >  2:2916814/1‑93 (MQ=255)                                                                                   tGATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAttt     <  1:3151423/93‑1 (MQ=255)                                                                                    gATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAtttt    <  2:2224631/93‑1 (MQ=255)                                                                                    gATCCCTCCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAtttt    >  2:1312177/1‑93 (MQ=255)                                                                                      tccctcccGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCTGGCGTTCTCCAtttttt  >  2:1423965/1‑91 (MQ=255)                                                                                      tccctcccGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtct  >  1:4543996/1‑93 (MQ=255)                                                                                           |                                                                                         TTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGAC‑GAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCT  >  NC_000913/1758171‑1758348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑