BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,762,307	T→C	Q35R (CAG→CGG)	ydiC ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,762,307	0	T	C	99.1%	338.6 / NA	111	Q35R (CAG→CGG)	ydiC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (59/51); minor base G (0/1); total (59/52)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TTGTCCGGCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCTGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCT  >  NC_000913/1762217‑1762396

ttGTCCGGCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGgccgc                                                                                         >  1:2947987/1‑93 (MQ=255)

 tGTCCGGCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGgccgct                                                                                        <  2:2171788/93‑1 (MQ=255)

  gTCCGGCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCtt                                                                                       >  1:175301/1‑93 (MQ=255)

  gTCCGGCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCtt                                                                                       >  2:1938339/1‑93 (MQ=255)

  gTCCGGCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCtt                                                                                       <  1:3984304/93‑1 (MQ=255)

     cGGCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGc                                                                                    <  1:3130613/93‑1 (MQ=255)

      ggCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGcc                                                                                   <  1:1524232/93‑1 (MQ=255)

      ggCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGcc                                                                                   >  2:3275398/1‑93 (MQ=255)

      ggCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGcc                                                                                   >  2:4600815/1‑93 (MQ=255)

      cgCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGcc                                                                                   <  1:971484/92‑1 (MQ=255)

       gCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGccc                                                                                  >  1:1364254/1‑92 (MQ=255)

         tCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGccacc                                                                                >  2:2206499/1‑93 (MQ=255)

          cGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGccacca                                                                               <  1:3951014/93‑1 (MQ=255)

           gCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAg                                                                              >  1:2188690/1‑93 (MQ=255)

            cTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGc                                                                             <  2:149555/93‑1 (MQ=255)

               acacTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCa                                                                          <  1:2803340/93‑1 (MQ=255)

               acacTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCa                                                                          >  2:1772872/1‑93 (MQ=255)

                cacTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCAc                                                                         >  1:1222124/1‑93 (MQ=255)

                 acTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACg                                                                        <  2:1910705/93‑1 (MQ=255)

                  cTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACgg                                                                       <  2:4367013/93‑1 (MQ=255)

                  cTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACgg                                                                       >  1:4655799/1‑93 (MQ=255)

                   tGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGCTTGCTTCACGCCTACGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGa                                                                      >  1:1751652/1‑93 (MQ=255)

                    gTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGAt                                                                     <  1:1628327/93‑1 (MQ=255)

                    gTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGAt                                                                     <  1:315324/93‑1 (MQ=255)

                     tCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGAtg                                                                    <  1:4476263/93‑1 (MQ=255)

                     tCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGAtg                                                                    <  1:551778/93‑1 (MQ=255)

                     tCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGAtg                                                                    >  2:1591606/1‑93 (MQ=255)

                          cacaTAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTAt                                                               <  2:823439/93‑1 (MQ=255)

                           acaTAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATc                                                              >  1:2640522/1‑93 (MQ=255)

                             aTAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATcgc                                                            >  2:1392300/1‑93 (MQ=255)

                              tAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATcgcc                                                           >  1:3955830/1‑93 (MQ=255)

                              tAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATcgcc                                                           >  1:86864/1‑93 (MQ=255)

                                gCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATcgccgc                                                         >  2:3880341/1‑93 (MQ=255)

                                     aGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCgg                                                    >  2:3094073/1‑93 (MQ=255)

                                      gCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCggg                                                   >  1:3102229/1‑93 (MQ=255)

                                       cccGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATCCCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGgt                                                  <  2:4148980/93‑1 (MQ=255)

                                       cccGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCgggg                                                  >  2:2188133/1‑92 (MQ=255)

                                       cccGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGgt                                                  >  2:2505863/1‑93 (MQ=255)

                                         cgcgCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGgtgt                                                >  2:3989576/1‑93 (MQ=255)

                                         cgcgCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGgtgt                                                >  1:2784681/1‑93 (MQ=255)

                                           cgcAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCa                                              <  2:1190673/93‑1 (MQ=255)

                                            gcAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAg                                             >  2:3850027/1‑93 (MQ=255)

                                             cAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGc                                            <  2:3581864/93‑1 (MQ=255)

                                               gCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGt                                          >  2:1806055/1‑93 (MQ=255)

                                               gCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGt                                          <  1:3595181/93‑1 (MQ=255)

                                                cccGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGtt                                         <  2:4396528/93‑1 (MQ=255)

                                                 ccGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTa                                        <  2:605138/93‑1 (MQ=255)

                                                 ccGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTa                                        >  2:266304/1‑93 (MQ=255)

                                                  cGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTaa                                       <  2:1198056/93‑1 (MQ=255)

                                                  cGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTaa                                       >  2:3214092/1‑93 (MQ=255)

                                                  cGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTaa                                       >  2:3090091/1‑93 (MQ=255)

                                                   gTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAg                                      >  2:1185620/1‑93 (MQ=255)

                                                      tGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCt                                   <  1:1608434/93‑1 (MQ=255)

                                                      tGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCt                                   <  2:816687/93‑1 (MQ=255)

                                                       gCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCtt                                  <  2:4486030/93‑1 (MQ=255)

                                                       gCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCtt                                  >  2:1975126/1‑93 (MQ=255)

                                                       gCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCtt                                  <  1:3880306/93‑1 (MQ=255)

                                                       gCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCtt                                  <  2:79617/93‑1 (MQ=255)

                                                       gCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCtt                                  >  1:2012116/1‑93 (MQ=255)

                                                        cTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTg                                 <  1:1287957/93‑1 (MQ=255)

                                                         ttCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGc                                >  1:4492061/1‑93 (MQ=255)

                                                          tCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGcc                               <  2:1274089/93‑1 (MQ=255)

                                                           cACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCa                              <  2:3888929/93‑1 (MQ=255)

                                                              gCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGc                           >  2:714152/1‑93 (MQ=255)

                                                                cTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGa                         >  1:855897/1‑93 (MQ=255)

                                                                 tAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGaa                        >  1:3576725/1‑93 (MQ=255)

                                                                 tAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGaa                        >  2:3308129/1‑93 (MQ=255)

                                                                  aaGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGaaa                       <  2:2594689/93‑1 (MQ=255)

                                                                  aaGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGaaa                       <  1:1353410/93‑1 (MQ=255)

                                                                  aaGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGaaa                       <  2:2594690/93‑1 (MQ=255)

                                                                  aaGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGaaa                       >  2:2294194/1‑93 (MQ=255)

                                                                  aaGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGaaa                       <  2:2021886/93‑1 (MQ=255)

                                                                   aGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAAt                      <  1:3646560/93‑1 (MQ=255)

                                                                    gcgcACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATc                     <  2:146536/93‑1 (MQ=255)

                                                                    gcgcACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATc                     <  1:2226844/93‑1 (MQ=255)

                                                                    gcgcACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATc                     >  1:2493622/1‑93 (MQ=255)

                                                                    gcgcACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGGGTCAGCGTTAAGCCTTGCCAGGCGAAATc                     >  2:4177793/1‑93 (MQ=255)

                                                                        aCGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTtgt                 <  1:2302308/93‑1 (MQ=255)

                                                                        aCGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTtgt                 >  2:2918155/1‑93 (MQ=255)

                                                                        aCGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTtgt                 <  2:4508112/93‑1 (MQ=255)

                                                                         cGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTtgtg                <  2:2834078/93‑1 (MQ=255)

                                                                          gCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTgg               >  2:1334618/1‑93 (MQ=255)

                                                                           ccGACCATACCCGGCGGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTggg              <  2:3668979/93‑1 (MQ=255)

                                                                           ccGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTggg              >  2:3257495/1‑93 (MQ=255)

                                                                           ccGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTggg              <  2:4309196/93‑1 (MQ=255)

                                                                            cGACCATCCCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGt             <  2:849089/93‑1 (MQ=255)

                                                                              aCCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTa           >  2:1558319/1‑93 (MQ=255)

                                                                               ccATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGGCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTAGGTTaa          >  2:3522507/1‑93 (MQ=255)

                                                                               ccATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTaa          <  2:2092150/93‑1 (MQ=255)

                                                                                cATGCCCGGCCGCTTTGCCACCAGCTCGCGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTaaa         <  2:3099914/93‑1 (MQ=255)

                                                                                cATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTaaa         >  2:3019119/1‑93 (MQ=255)

                                                                                cATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTaaa         <  1:564603/93‑1 (MQ=255)

                                                                                 aTACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTTAAg        >  1:2547640/1‑93 (MQ=255)

                                                                                 aTACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAg        <  2:1392386/93‑1 (MQ=255)

                                                                                 aTACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAg        <  2:1377674/93‑1 (MQ=255)

                                                                                  tACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAgg       >  2:2431669/1‑93 (MQ=255)

                                                                                    cccGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGtt     <  1:1107425/93‑1 (MQ=255)

                                                                                      cGGCCGCTTTGCCACCAGCTCACGGCTGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTcc   >  1:4644891/1‑93 (MQ=255)

                                                                                      cGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTcc   >  1:2024745/1‑93 (MQ=255)

                                                                                       ggCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCt  >  1:104867/1‑93 (MQ=255)

TTGTCCGGCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCTGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCT  >  NC_000913/1762217‑1762396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`