Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,768,647	T→C	A12A (GCT→GCC)	b1689 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,768,647	0	T	C	100.0%	291.1 / NA	91	A12A (GCT→GCC)	b1689	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (63/28);  total (63/28)

TACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTG  >  NC_000913/1768559‑1768736                                                                                         |                                                                                          tACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATg                                                                                       >  2:1872965/1‑93 (MQ=255) tACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATg                                                                                       <  2:3767331/93‑1 (MQ=255)   cTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGaa                                                                                     <  1:1124163/93‑1 (MQ=255)    tgtgACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAAc                                                                                    <  2:3441855/93‑1 (MQ=255)     gtgACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACt                                                                                   >  1:3787138/1‑93 (MQ=255)      tgACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTc                                                                                  <  1:3995597/93‑1 (MQ=255)      tgACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTc                                                                                  >  2:1567572/1‑93 (MQ=255)       gACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTcc                                                                                 >  2:1242707/1‑93 (MQ=255)        aCTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCa                                                                                >  2:3959300/1‑93 (MQ=255)          tctctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAgg                                                                              >  2:4354626/1‑93 (MQ=255)          tctctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAgg                                                                              <  2:4095483/93‑1 (MQ=255)           ctctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGc                                                                             >  1:4013453/1‑93 (MQ=255)            tctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCa                                                                            <  1:4397129/93‑1 (MQ=255)            tctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCa                                                                            <  1:2631030/93‑1 (MQ=255)            tctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCa                                                                            <  2:2701130/93‑1 (MQ=255)             ctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCAc                                                                           <  1:4673634/93‑1 (MQ=255)               tCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTc                                                                         >  2:2034775/1‑93 (MQ=255)                 aaGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCg                                                                       >  2:2528819/1‑93 (MQ=255)                     cGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCat                                                                   >  2:3206164/1‑93 (MQ=255)                       tCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCatat                                                                 >  1:343779/1‑93 (MQ=255)                          gcagGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATAtttt                                                              >  1:2792297/1‑93 (MQ=255)                            agGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTg                                                            >  2:3223018/1‑93 (MQ=255)                             ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGc                                                           >  1:323515/1‑93 (MQ=255)                             ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGc                                                           >  1:4401589/1‑93 (MQ=255)                              gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGcc                                                          >  1:4198189/1‑93 (MQ=255)                               cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCa                                                         >  2:3836381/1‑93 (MQ=255)                               cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCa                                                         >  2:3188037/1‑93 (MQ=255)                                tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCAt                                                        >  1:173550/1‑93 (MQ=255)                                 gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATg                                                       >  2:3516644/1‑93 (MQ=255)                                  aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa                                                      >  1:4520866/1‑93 (MQ=255)                                  aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa                                                      >  1:1628457/1‑93 (MQ=255)                                   tAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAc                                                     <  2:986784/93‑1 (MQ=255)                                   tAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAc                                                     >  1:2948361/1‑93 (MQ=255)                                     aTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTa                                                   <  2:1822664/93‑1 (MQ=255)                                      tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt                                                  >  1:1578798/1‑93 (MQ=255)                                      tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt                                                  >  2:3663752/1‑93 (MQ=255)                                         tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga                                               >  1:242114/1‑93 (MQ=255)                                          atttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat                                              <  1:3693368/93‑1 (MQ=255)                                          atttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat                                              <  1:3084280/93‑1 (MQ=255)                                          atttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat                                              >  1:926754/1‑93 (MQ=255)                                          atttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat                                              >  1:4604830/1‑93 (MQ=255)                                           tttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatg                                             >  1:930068/1‑93 (MQ=255)                                             tataTTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGaa                                           >  1:1803883/1‑93 (MQ=255)                                              atatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAt                                          >  2:3462329/1‑93 (MQ=255)                                              atatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAt                                          <  2:1016070/93‑1 (MQ=255)                                              atatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAt                                          >  2:4677048/1‑93 (MQ=255)                                                atTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc                                        <  2:538897/93‑1 (MQ=255)                                                 ttttATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcg                                       >  1:311374/1‑93 (MQ=255)                                                  tttATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcgc                                      <  2:4307652/93‑1 (MQ=255)                                                   ttATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg                                     <  1:3522084/93‑1 (MQ=255)                                                   ttATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg                                     >  1:4086216/1‑93 (MQ=255)                                                    tATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGa                                    <  1:3029851/93‑1 (MQ=255)                                                     aTTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAc                                   >  1:1726715/1‑93 (MQ=255)                                                     aTTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAc                                   >  2:885051/1‑93 (MQ=255)                                                      ttGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAcc                                  >  1:3135438/1‑93 (MQ=255)                                                       tGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCt                                 >  1:3935235/1‑93 (MQ=255)                                                       tGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCt                                 <  1:557087/93‑1 (MQ=255)                                                          ttATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGa                              >  2:2960803/1‑93 (MQ=255)                                                          ttATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGa                              >  1:671474/1‑93 (MQ=255)                                                          ttATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGa                              >  2:1185128/1‑93 (MQ=255)                                                           tATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAt                             <  1:3127438/93‑1 (MQ=255)                                                            aTTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAtt                            <  2:4321353/93‑1 (MQ=255)                                                            aTTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAtt                            <  2:4408093/93‑1 (MQ=255)                                                             tttCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTg                           >  1:3363199/1‑93 (MQ=255)                                                             tttCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTg                           >  2:244738/1‑93 (MQ=255)                                                             tttCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTg                           >  2:3180777/1‑93 (MQ=255)                                                              ttCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGc                          >  2:1529885/1‑93 (MQ=255)                                                               tCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGcc                         >  1:573724/1‑93 (MQ=255)                                                               tCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGcc                         >  2:2752378/1‑93 (MQ=255)                                                                cATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc                        <  1:2270623/93‑1 (MQ=255)                                                                 aTGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCa                       >  1:2247794/1‑93 (MQ=255)                                                                   gCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGa                     >  2:4401870/1‑93 (MQ=255)                                                                    cAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAc                    <  1:1394869/93‑1 (MQ=255)                                                                     aGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAcc                   >  2:1956146/1‑93 (MQ=255)                                                                      gCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCg                  >  2:4462015/1‑93 (MQ=255)                                                                      gCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCg                  <  1:3994982/93‑1 (MQ=255)                                                                        cATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGt                >  1:3630375/1‑93 (MQ=255)                                                                         aTTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg               >  1:1986954/1‑93 (MQ=255)                                                                         aTTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg               >  1:2119727/1‑93 (MQ=255)                                                                          ttACACGATGAACTCATATGAACCCCAGTCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCCACCTGGATTGCCCAGACCGGTGa              <  2:3736607/93‑1 (MQ=255)                                                                          ttACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGa              >  2:3033759/1‑93 (MQ=255)                                                                          ttACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGCGa              >  2:2233357/1‑93 (MQ=255)                                                                           tACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAc             <  2:1694263/93‑1 (MQ=255)                                                                            acacGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACa            >  1:3628523/1‑93 (MQ=255)                                                                            acacGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACa            >  2:2236696/1‑93 (MQ=255)                                                                             cacGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAg           >  2:690664/1‑93 (MQ=255)                                                                              acGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt          <  1:4232106/93‑1 (MQ=255)                                                                              acGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt          >  2:2879094/1‑93 (MQ=255)                                                                                gATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGa        <  1:2674912/93‑1 (MQ=255)                                                                                   gAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAg     >  1:1585998/1‑93 (MQ=255)                                                                                      cGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTg  >  1:4201779/1‑93 (MQ=255)                                                                                      cGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTg  >  1:1010793/1‑93 (MQ=255)                                                                                         |                                                                                          TACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTG  >  NC_000913/1768559‑1768736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑