BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,191	T→C	R325R (CGT→CGC)	b1695 →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,191	0	T	C	99.1%	332.4 / NA	108	R325R (CGT→CGC)	b1695	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (43/64); minor base A (1/0); total (44/64)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGA  >  NC_000913/1776101‑1776280

gcgcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTgcacgcac                                                                                         <  2:462732/93‑1 (MQ=255)

 cgcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCAcc                                                                                        <  2:916429/93‑1 (MQ=255)

 cgcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCAcc                                                                                        <  2:684430/93‑1 (MQ=255)

 cgcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCAcc                                                                                        >  1:264527/1‑93 (MQ=255)

  gcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCg                                                                                       <  2:249469/93‑1 (MQ=255)

  gcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACCCACCg                                                                                       >  2:389751/1‑93 (MQ=255)

     aCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCaa                                                                                    >  1:950383/1‑93 (MQ=255)

     aCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCaa                                                                                    >  1:4514902/1‑93 (MQ=255)

      cATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAAt                                                                                   >  2:1302234/1‑93 (MQ=255)

       aTGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATg                                                                                  >  1:4234218/1‑93 (MQ=255)

        tGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCCCCGCAATgg                                                                                 >  1:3612814/1‑93 (MQ=255)

        tGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATgg                                                                                 >  2:2967581/1‑93 (MQ=255)

        tGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATgg                                                                                 >  2:4283234/1‑93 (MQ=255)

          gTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGaa                                                                               <  2:24274/93‑1 (MQ=255)

          gTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGaa                                                                               <  2:2497828/93‑1 (MQ=255)

           ggCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAg                                                                              <  1:2409400/92‑1 (MQ=255)

           ggCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAg                                                                              <  1:4178887/92‑1 (MQ=255)

            gCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGaact                                                                             <  2:4488132/93‑3 (MQ=255)

            gCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGt                                                                             >  1:3226232/1‑93 (MQ=255)

            gCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGt                                                                             >  2:59555/1‑93 (MQ=255)

              tGAACGTGGCACGCAAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGAAATGGAAGTCa                                                                           <  2:3472929/93‑1 (MQ=255)

              tGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCa                                                                           <  1:2616426/93‑1 (MQ=255)

              tGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCa                                                                           >  1:452718/1‑93 (MQ=255)

              tGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCa                                                                           >  2:1366759/1‑93 (MQ=255)

              tGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCACAATGGAAGTCa                                                                           >  2:3588622/1‑93 (MQ=255)

              tGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCACTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCa                                                                           <  2:952500/93‑1 (MQ=255)

               gAAAGTGGCTGGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAt                                                                          <  1:3804713/93‑1 (MQ=255)

               gAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAt                                                                          >  2:129914/1‑93 (MQ=255)

               gAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAt                                                                          <  1:1388763/93‑1 (MQ=255)

               gAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAt                                                                          <  1:1199966/93‑1 (MQ=255)

               gAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAt                                                                          <  1:4644597/93‑1 (MQ=255)

                aaaGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAtt                                                                         <  2:2165497/93‑1 (MQ=255)

                aaaGTGGCATGCCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAtt                                                                         <  2:2084592/93‑1 (MQ=255)

                 aaGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtg                                                                        <  1:3569389/93‑1 (MQ=255)

                  aGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtga                                                                       <  1:3869297/93‑1 (MQ=255)

                  aGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtga                                                                       >  2:3594287/1‑93 (MQ=255)

                   gTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgat                                                                      <  2:4378980/93‑1 (MQ=255)

                   gTGGCAGGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgat                                                                      <  2:1173849/93‑1 (MQ=255)

                      gcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTgatgat                                                                   <  1:2065843/93‑1 (MQ=255)

                      gcatggaaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTgatgat                                                                   <  2:2656079/93‑1 (MQ=255)

                       catggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGatgatg                                                                  <  2:219493/93‑1 (MQ=255)

                        atggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGc                                                                 <  2:3624706/93‑1 (MQ=255)

                        atggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGAACCGCAATGGAAGTCATTGATGATGc                                                                 >  2:2927522/1‑93 (MQ=255)

                        aggacaAGCCGATCATCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGc                                                                 <  1:3789888/89‑1 (MQ=255)

                             aaGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTc                                                            <  1:2395176/93‑1 (MQ=255)

                             aaGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTc                                                            >  2:2835968/1‑93 (MQ=255)

                              aGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCa                                                           >  1:2976812/1‑93 (MQ=255)

                                ccGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCaaa                                                         <  2:3838838/93‑1 (MQ=255)

                                ccGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCaaa                                                         >  2:1472558/1‑93 (MQ=255)

                                ccGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCaaa                                                         <  2:1867447/93‑1 (MQ=255)

                                ccGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCaaa                                                         <  2:4279189/93‑1 (MQ=255)

                                 cGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAAt                                                        <  2:1073675/93‑1 (MQ=255)

                                 cGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAAt                                                        <  1:2945839/93‑1 (MQ=255)

                                    tCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     <  1:6034/93‑1 (MQ=255)

                                    tCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     >  2:2439842/1‑93 (MQ=255)

                                    tCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     <  1:3905853/93‑1 (MQ=255)

                                     cAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATg                                                    <  1:940465/93‑1 (MQ=255)

                                     cAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATg                                                    <  1:165321/93‑1 (MQ=255)

                                        cATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGc                                                 <  2:2103005/93‑1 (MQ=255)

                                         aTCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCg                                                >  2:4202842/1‑93 (MQ=255)

                                         aTCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCg                                                >  1:3055165/1‑93 (MQ=255)

                                         aTCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCg                                                >  2:350489/1‑93 (MQ=255)

                                           cAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCggc                                              >  1:1154387/1‑92 (MQ=255)

                                               cACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTgg                                          <  1:2004653/93‑1 (MQ=255)

                                                aCTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTggg                                         >  2:2915740/1‑93 (MQ=255)

                                                 cTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGc                                        <  2:3418315/93‑1 (MQ=255)

                                                     gcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCtata                                    <  1:2628812/93‑1 (MQ=255)

                                                     gcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCtata                                    <  2:790999/93‑1 (MQ=255)

                                                     gcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCtata                                    <  2:2009504/93‑1 (MQ=255)

                                                     gcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCtata                                    <  1:2588742/93‑1 (MQ=255)

                                                     gcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCtata                                    <  1:3254350/93‑1 (MQ=255)

                                                      cACCAGGTCGCCGCTGACAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAACCATGGGCGGTCTGGGCTATAc                                   <  2:2455122/93‑1 (MQ=255)

                                                       aCCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATAcc                                  >  1:3969818/1‑93 (MQ=255)

                                                         cAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGa                                <  2:3216229/93‑1 (MQ=255)

                                                          aGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGAt                               <  1:1950502/93‑1 (MQ=255)

                                                              cggcgCTGGCAAAACTGTACTGTGGACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAgg                           >  2:4518213/1‑93 (MQ=255)

                                                               ggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgc                          >  2:810921/1‑93 (MQ=255)

                                                                gcgcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcg                         >  2:1798903/1‑93 (MQ=255)

                                                                 cgcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgc                        <  1:972663/93‑1 (MQ=255)

                                                                  gcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcg                       >  1:2486646/1‑93 (MQ=255)

                                                                   cTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcgc                      >  2:4188164/1‑93 (MQ=255)

                                                                    tGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGcgcgcg                     >  1:4221289/1‑93 (MQ=255)

                                                                    tGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGcgcgcg                     >  2:3983170/1‑93 (MQ=255)

                                                                        aaaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtctc                 <  2:1992397/93‑1 (MQ=255)

                                                                        aaaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtctc                 <  2:3468048/93‑1 (MQ=255)

                                                                         aaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTcc                >  1:1909694/1‑93 (MQ=255)

                                                                          aaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTccc               >  2:2754532/1‑93 (MQ=255)

                                                                          aaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTccc               <  2:3323272/93‑1 (MQ=255)

                                                                          aaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTccc               <  2:564784/93‑1 (MQ=255)

                                                                           actgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCg              <  2:3687798/93‑1 (MQ=255)

                                                                               tactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTc          >  1:165009/1‑93 (MQ=255)

                                                                               tactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTc          <  2:3726756/93‑1 (MQ=255)

                                                                               tactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTc          >  2:370925/1‑93 (MQ=255)

                                                                               tactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTc          <  2:208023/93‑1 (MQ=255)

                                                                                  tgtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTgg       >  1:3139422/1‑93 (MQ=255)

                                                                                    tgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCg     >  1:1091338/1‑93 (MQ=255)

                                                                                     gcacgcacCGCAATGGAAGTCATTGATGATGCTATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGt    <  1:2331824/93‑1 (MQ=255)

                                                                                     gcacgcacCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGt    <  1:1708777/93‑1 (MQ=255)

                                                                                       aCGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGa  <  2:534731/93‑1 (MQ=255)

                                                                                       aCGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGa  <  2:411896/93‑1 (MQ=255)

GCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGA  >  NC_000913/1776101‑1776280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`