BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,805,274	T→C	L295P (CTG→CCG)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,805,274	0	T	C	98.6%	416.9 / ‑2.7	138	L295P (CTG→CCG)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (78/58); total (79/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.85e-01

Only 100 of 141 total aligned reads displayed.

TGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACC  >  NC_000913/1805184‑1805363

   aaCTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCt                                                                                      >  1:3364387/1‑93 (MQ=255)

   aaCTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCt                                                                                      >  2:2526778/1‑93 (MQ=255)

      tGGCAGTAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTccc                                                                                   >  2:2828492/1‑93 (MQ=255)

      tGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTccc                                                                                   >  1:2989928/1‑93 (MQ=255)

       ggCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCa                                                                                  <  1:453029/93‑1 (MQ=255)

       ggCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCa                                                                                  <  2:733879/93‑1 (MQ=255)

       ggCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCa                                                                                  <  2:1513064/93‑1 (MQ=255)

       ggCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCGGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGGTCCCa                                                                                  <  2:2910976/93‑1 (MQ=255)

         cAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGCGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATg                                                                                >  1:2399176/1‑93 (MQ=255)

         cAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTCTGGCGTAGCTGCGGGGAGAGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATg                                                                                >  2:4685252/1‑93 (MQ=255)

          aGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGa                                                                               <  1:1525585/93‑1 (MQ=255)

             aaaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGa                                                                            <  2:1026694/93‑1 (MQ=255)

             aaaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGa                                                                            <  1:4146013/93‑1 (MQ=255)

             aaaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGa                                                                            >  2:3113637/1‑93 (MQ=255)

              aaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGAt                                                                           >  1:2850030/1‑93 (MQ=255)

                tGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGTTCCCATGACGAtcc                                                                         >  1:3766169/1‑91 (MQ=255)

                tGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATAc                                                                         <  2:3300553/93‑1 (MQ=255)

                tGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATAc                                                                         >  1:4631795/1‑93 (MQ=255)

                 gCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACg                                                                        >  1:2870373/1‑93 (MQ=255)

                    tctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCaa                                                                     >  2:2681329/1‑93 (MQ=255)

                    tctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCaa                                                                     >  2:4516936/1‑93 (MQ=255)

                    tctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCaa                                                                     >  2:492197/1‑93 (MQ=255)

                     ctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCaaa                                                                    <  1:2880279/93‑1 (MQ=255)

                     ctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCaaa                                                                    <  1:4300540/93‑1 (MQ=255)

                      tctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCaaaa                                                                   >  2:490102/1‑93 (MQ=255)

                      tctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCaaaa                                                                   <  1:3884326/93‑1 (MQ=255)

                       ctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCaaaaa                                                                  <  1:3715616/93‑1 (MQ=255)

                          gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAAtt                                                               <  1:1824686/93‑1 (MQ=255)

                           aaGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAAttt                                                              >  1:1343463/1‑93 (MQ=255)

                             gagaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTAc                                                            >  2:2412813/1‑93 (MQ=255)

                             gagaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTAc                                                            >  2:442182/1‑93 (MQ=255)

                             gagaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTAc                                                            <  2:3689220/93‑1 (MQ=255)

                             gagaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTAc                                                            >  2:863069/1‑93 (MQ=255)

                             aagaTGGTTCGTTTTGGCGTAGCGGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTAc                                                            <  2:3294367/92‑1 (MQ=255)

                              agaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACg                                                           <  1:1670756/93‑1 (MQ=255)

                              agaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACg                                                           >  2:2442996/1‑93 (MQ=255)

                               gaTGTTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGc                                                          <  2:32200/93‑1 (MQ=255)

                               gaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGc                                                          >  2:2930091/1‑93 (MQ=255)

                                aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCt                                                         <  1:730907/93‑1 (MQ=255)

                                 tGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCtt                                                        <  1:4641779/93‑1 (MQ=255)

                                   gTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTAc                                                      >  1:4081443/1‑93 (MQ=255)

                                   gTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTAc                                                      >  2:4049157/1‑93 (MQ=255)

                                    ttCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTAcc                                                     >  1:2411333/1‑93 (MQ=255)

                                     tCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCt                                                    <  2:3880728/93‑1 (MQ=255)

                                     tCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCt                                                    >  2:1624740/1‑93 (MQ=255)

                                     tCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCt                                                    >  2:1534422/1‑93 (MQ=255)

                                      cGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCtt                                                   <  2:3513416/93‑1 (MQ=255)

                                        ttttGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTc                                                 <  2:2158505/93‑1 (MQ=255)

                                        ttttGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTc                                                 >  1:2012436/1‑93 (MQ=255)

                                          ttGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTccc                                               <  2:3685898/93‑1 (MQ=255)

                                            ggCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGc                                             <  2:2088602/93‑1 (MQ=255)

                                               gTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTaa                                          <  1:1710852/93‑1 (MQ=255)

                                               gTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTaa                                          >  2:2730038/1‑93 (MQ=255)

                                                tAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAAc                                         >  1:557196/1‑93 (MQ=255)

                                                 aGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACa                                        <  1:1892261/93‑1 (MQ=255)

                                                   cTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaa                                      >  2:4658791/1‑93 (MQ=255)

                                                   cTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaa                                      >  2:636246/1‑93 (MQ=255)

                                                    tGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaa                                     <  1:793716/93‑1 (MQ=255)

                                                     gCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaaa                                    >  1:2249915/1‑93 (MQ=255)

                                                     gCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaaa                                    >  1:3230216/1‑93 (MQ=255)

                                                     gCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCCTGCTCCCATGACGATACGCAAAAAATTTACGCTTATCTTTCCCGCTTACaaaaa                                    >  2:4440873/1‑93 (MQ=255)

                                                      cGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAAc                                   <  2:4206542/93‑1 (MQ=255)

                                                       ggggAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACa                                  <  1:4620931/93‑1 (MQ=255)

                                                       ggggAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACa                                  <  2:3467475/93‑1 (MQ=255)

                                                        gggAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAt                                 >  1:3355917/1‑93 (MQ=255)

                                                        gggAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAt                                 <  2:2286438/93‑1 (MQ=255)

                                                        gggAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAt                                 <  2:2911036/93‑1 (MQ=255)

                                                        gggAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAt                                 <  2:1207578/93‑1 (MQ=255)

                                                         ggAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAtt                                <  2:3572539/93‑1 (MQ=255)

                                                         ggAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAtt                                >  2:1866266/1‑93 (MQ=255)

                                                          gAGTGCAGCTACACTCAATCAGGGAACACGTCCGTGCCCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTc                               <  2:2881603/93‑1 (MQ=255)

                                                          gAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTc                               <  1:3237484/93‑1 (MQ=255)

                                                          gAGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTc                               <  2:1860811/93‑1 (MQ=255)

                                                           aGTGCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTcc                              >  2:3751463/1‑93 (MQ=255)

                                                              gCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACGTTccccc                           <  1:510825/93‑1 (MQ=255)

                                                              gCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTccccc                           >  2:3791132/1‑93 (MQ=255)

                                                              gCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTccccc                           >  2:331289/1‑93 (MQ=255)

                                                              gCAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTccccc                           >  2:4583041/1‑93 (MQ=255)

                                                              gCAGCCACACTCAATCAGGGAACACGTACGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTccccc                           >  1:3950234/1‑93 (MQ=255)

                                                               cAGCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCa                          <  1:4597171/93‑1 (MQ=255)

                                                                 gCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGc                        >  1:4489950/1‑93 (MQ=255)

                                                                 gCCACACTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGc                        >  1:2039151/1‑93 (MQ=255)

                                                                    acacTCAATCAGGGAACACGTCCGTTCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCAtt                     <  2:3923592/93‑1 (MQ=255)

                                                                    acacTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCAtt                     >  2:3374634/1‑93 (MQ=255)

                                                                     cacTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTg                    <  1:874980/93‑1 (MQ=255)

                                                                     cacTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTg                    >  2:1319703/1‑93 (MQ=255)

                                                                      acTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTgg                   >  1:310945/1‑93 (MQ=255)

                                                                      acTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTgg                   >  2:234571/1‑93 (MQ=255)

                                                                       cTCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggg                  >  1:4142790/1‑93 (MQ=255)

                                                                        tCAATCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTgggg                 <  2:854407/93‑1 (MQ=255)

                                                                          aaTCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGa               <  1:2629464/93‑1 (MQ=255)

                                                                            tCAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAat             >  1:3452610/1‑93 (MQ=255)

                                                                             cAGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatc            <  2:3548059/93‑1 (MQ=255)

                                                                              aGGGAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatca           >  2:438648/1‑93 (MQ=255)

                                                                                ggAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatcatc         <  2:1886169/93‑1 (MQ=255)

                                                                                ggAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatcatc         <  1:2622155/93‑1 (MQ=255)

                                                                                 gAACACGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAAtcatca        >  1:2557760/1‑93 (MQ=255)

                                                                                     acGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCaa    <  1:2377603/93‑1 (MQ=255)

                                                                                     acGTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCaa    >  1:3366610/1‑93 (MQ=255)

                                                                                       gTCCGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAAcc  >  1:3627628/1‑93 (MQ=255)

TGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACC  >  NC_000913/1805184‑1805363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`