BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,866,382	G→A	G484D (GGC→GAC)	yeaG →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,866,382	0	G	A	99.2%	439.6 / ‑5.3	123	G484D (GGC→GAC)	yeaG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (47/75); total (47/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

Only 100 of 125 total aligned reads displayed.

TTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGA  >  NC_000913/1866292‑1866471

ttGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACa                                                                                         <  2:3911635/93‑1 (MQ=255)

 tGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACaa                                                                                        <  1:3920667/93‑1 (MQ=255)

  gAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACaaa                                                                                       <  2:2991185/93‑1 (MQ=255)

  gAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACaaa                                                                                       <  2:3110594/93‑1 (MQ=255)

  gAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACaaa                                                                                       <  1:2083634/93‑1 (MQ=255)

   aGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAag                                                                                      <  1:508482/93‑1 (MQ=255)

   aGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAag                                                                                      <  1:3300899/93‑1 (MQ=255)

    gcgcGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAaga                                                                                     <  2:2735899/93‑1 (MQ=255)

       cgAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGAt                                                                                  <  2:378508/93‑1 (MQ=255)

        gAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATc                                                                                 <  2:2139234/93‑1 (MQ=255)

         aGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATcc                                                                                <  2:1600967/93‑1 (MQ=255)

           cAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAg                                                                              <  2:3963483/93‑1 (MQ=255)

            aGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAATAGATCCAGa                                                                             <  1:4682344/93‑1 (MQ=255)

             gTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGAc                                                                            <  1:3248020/93‑1 (MQ=255)

             gTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGAc                                                                            <  1:485745/93‑1 (MQ=255)

              ttCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACg                                                                           <  2:4589330/93‑1 (MQ=255)

               tCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACgg                                                                          <  1:1616080/93‑1 (MQ=255)

                cccACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGc                                                                         >  2:2411536/1‑93 (MQ=255)

                cccACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGc                                                                         <  2:3486493/93‑1 (MQ=255)

                    caAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTAc                                                                     <  1:3061926/93‑1 (MQ=255)

                     aaGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTAcc                                                                    <  1:2309596/93‑1 (MQ=255)

                     aaGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTAcc                                                                    >  2:1340875/1‑93 (MQ=255)

                      agagCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCt                                                                   <  2:3422784/93‑1 (MQ=255)

                       gagCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCtt                                                                  >  2:475793/1‑93 (MQ=255)

                       gagCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCtt                                                                  <  2:1126299/93‑1 (MQ=255)

                       gagCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCtt                                                                  <  1:3656219/93‑1 (MQ=255)

                         gcaggcagAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGa                                                                >  2:3658913/1‑93 (MQ=255)

                          caggcagAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGaa                                                               <  2:362185/93‑1 (MQ=255)

                          caggcagAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGaa                                                               <  2:1862704/93‑1 (MQ=255)

                          caggcagAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGaa                                                               <  2:4669921/93‑1 (MQ=255)

                           aggcagAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAAt                                                              <  1:1354891/93‑1 (MQ=255)

                           aggcagAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAAt                                                              <  2:2086977/93‑1 (MQ=255)

                            ggcagAACTCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATCCCGAGTTTATCGACAAAGAGATCCAGACGCCCTACCTTGAATc                                                             <  1:1929236/93‑1 (MQ=255)

                            ggcagAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATc                                                             >  2:4334974/1‑93 (MQ=255)

                             gcagAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATcc                                                            <  1:4339085/93‑1 (MQ=255)

                             gcagAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATcc                                                            <  2:468501/93‑1 (MQ=255)

                               agAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTa                                                          <  2:3773116/93‑1 (MQ=255)

                               agAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTa                                                          <  2:2554244/93‑1 (MQ=255)

                                gAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTAt                                                         <  1:3630179/93‑1 (MQ=255)

                                gAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTAt                                                         >  1:2019328/1‑93 (MQ=255)

                                gAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTAt                                                         <  1:4237517/93‑1 (MQ=255)

                                  aCGCTATCTGGAGTGCCTGAAAGGTTATCTGATCCCGAAAGATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTc                                                       <  1:851648/93‑1 (MQ=255)

                                    gCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCg                                                     <  1:1281960/93‑1 (MQ=255)

                                     cTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGa                                                    <  2:2018292/93‑1 (MQ=255)

                                      tATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGaa                                                   >  2:1488694/1‑93 (MQ=255)

                                       aTCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAat                                                  >  1:2297625/1‑93 (MQ=255)

                                       aTCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAat                                                  >  1:3145218/1‑93 (MQ=255)

                                        tCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAata                                                 <  2:2415742/93‑1 (MQ=255)

                                          tGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATg                                               <  1:2449928/93‑1 (MQ=255)

                                           ggAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATgg                                              <  2:4484065/93‑1 (MQ=255)

                                           ggAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATgg                                              >  2:1666895/1‑93 (MQ=255)

                                            gAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATggg                                             <  1:2536968/93‑1 (MQ=255)

                                            gAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATggg                                             >  1:2084387/1‑93 (MQ=255)

                                             aGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGc                                            <  2:640151/93‑1 (MQ=255)

                                             aGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGc                                            <  2:1652371/93‑1 (MQ=255)

                                               ttCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCaa                                          >  1:1741609/1‑93 (MQ=255)

                                               ttCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCaa                                          >  1:363613/1‑93 (MQ=255)

                                                tCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCaaa                                         >  1:1150619/1‑93 (MQ=255)

                                                tCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCaaa                                         <  1:373665/93‑1 (MQ=255)

                                                   tGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACa                                      >  2:3358630/1‑93 (MQ=255)

                                                     aaaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACAtt                                    <  2:1811827/93‑1 (MQ=255)

                                                      aaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACAttt                                   <  2:1530026/93‑1 (MQ=255)

                                                      aaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACAttt                                   >  2:679356/1‑93 (MQ=255)

                                                      aaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACAttt                                   <  2:2655279/93‑1 (MQ=255)

                                                       aGGTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACAtttt                                  >  2:1891876/1‑93 (MQ=255)

                                                        ggTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTc                                 <  2:4037879/93‑1 (MQ=255)

                                                        ggTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTc                                 <  2:245415/93‑1 (MQ=255)

                                                        agTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCTTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTc                                 <  2:2329605/92‑1 (MQ=255)

                                                        agTTATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTc                                 <  2:1575402/92‑1 (MQ=255)

                                                          ttATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGa                               >  1:362583/1‑93 (MQ=255)

                                                          ttATCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGa                               >  1:905490/1‑93 (MQ=255)

                                                            aTCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGAcc                             >  1:1356985/1‑93 (MQ=255)

                                                             tCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCg                            >  1:3079684/1‑93 (MQ=255)

                                                             tCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCg                            >  1:4380331/1‑93 (MQ=255)

                                                             tCTGATCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCg                            >  1:3902426/1‑93 (MQ=255)

                                                                  tCCCGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATg                       >  2:1048194/1‑93 (MQ=255)

                                                                   cccGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGt                      <  2:3058646/93‑1 (MQ=255)

                                                                    ccGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGtt                     >  1:2618403/1‑93 (MQ=255)

                                                                    ccGAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGtt                     >  2:2671387/1‑93 (MQ=255)

                                                                      gAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTAc                   <  2:4649448/93‑1 (MQ=255)

                                                                      gAAATATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTAc                   >  1:859870/1‑93 (MQ=255)

                                                                        aaTATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCt                 <  2:2995105/93‑1 (MQ=255)

                                                                        aaTATGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCt                 >  2:1124560/1‑93 (MQ=255)

                                                                         atatGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTa                >  2:2760366/1‑93 (MQ=255)

                                                                         atatGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTa                >  1:1549550/1‑93 (MQ=255)

                                                                         atatGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTa                <  2:4243076/93‑1 (MQ=255)

                                                                            tGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTAcgc             <  1:2428256/93‑1 (MQ=255)

                                                                            tGCCGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTAcgc             >  1:3425204/1‑93 (MQ=255)

                                                                            tGCCGAGTTTATCGACAAAGAGAGCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTAcgc             <  1:1800927/93‑1 (MQ=255)

                                                                               cGAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGa          >  2:1155367/1‑93 (MQ=255)

                                                                                gAGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGAt         >  1:3357066/1‑93 (MQ=255)

                                                                                 aGTTTATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGAtt        >  1:3400231/1‑93 (MQ=255)

                                                                                   tttATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTc      <  2:2198081/93‑1 (MQ=255)

                                                                                    ttATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCt     <  1:2552556/93‑1 (MQ=255)

                                                                                    ttATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCt     >  2:683436/1‑93 (MQ=255)

                                                                                     tATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTTCGCGGATTTCTg    <  1:3779951/93‑1 (MQ=255)

                                                                                     tATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTg    <  1:4563848/93‑1 (MQ=255)

                                                                                     tATCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTg    >  1:4295708/1‑93 (MQ=255)

                                                                                       tCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGa  <  1:2667841/93‑1 (MQ=255)

                                                                                       tCGACAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGa  <  2:938652/93‑1 (MQ=255)

TTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGA  >  NC_000913/1866292‑1866471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`