BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,883,199	C→T	L171L (CTG→TTG)	yeaW →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,883,199	0	C	T	99.1%	399.9 / NA	112	L171L (CTG→TTG)	yeaW	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (57/54); minor base G (0/1); total (57/55)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

CTGGTTCCGGTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCCTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTGCCAACTGGAAG  >  NC_000913/1883109‑1883288

cTGGTTCCGGTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTg                                                                                         >  2:3501335/1‑93 (MQ=255)

cTGGTTCCGGTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTg                                                                                         >  1:1911002/1‑93 (MQ=255)

 tGGTTCCGGTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTgg                                                                                        >  2:1762753/1‑93 (MQ=255)

 tGGTTCCGGTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTgg                                                                                        >  1:2449492/1‑93 (MQ=255)

  ggTTCCGGTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTggg                                                                                       <  1:464234/93‑1 (MQ=255)

   gTTCCGGTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGgc                                                                                      <  1:3605541/93‑1 (MQ=255)

   gTTCCGGTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGgc                                                                                      >  2:3923585/1‑93 (MQ=255)

      ccGGTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGcgcg                                                                                   >  1:3241749/1‑93 (MQ=255)

        ggTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGaa                                                                                 <  2:2260996/93‑1 (MQ=255)

         gtgtgtCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGaaa                                                                                <  2:2174221/93‑1 (MQ=255)

         gTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGaaa                                                                                >  1:3395520/1‑93 (MQ=255)

         gTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGaaa                                                                                <  2:281204/93‑1 (MQ=255)

         gTGCGCCTGGACGAATTTGCCGGCTTTGACTTCATCAACATGGACCCCAGCGCCTCGAGCGTAGCAGATCAATTACCCGGCTTGGGCGCGaaa                                                                                <  2:4678197/93‑1 (MQ=255)

          tGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAg                                                                               <  1:602446/93‑1 (MQ=255)

           gCGTCTGGAAGAATATTCCGGATTCGCCTTCATCAACATGGCCCCCAACGCCACAAGCTTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGt                                                                              <  2:4500747/93‑1 (MQ=255)

           gCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGt                                                                              <  1:1358094/93‑1 (MQ=255)

             gTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGc                                                                            >  2:2072316/1‑93 (MQ=255)

             gTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGc                                                                            >  2:2109985/1‑93 (MQ=255)

              tCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCt                                                                           >  1:4608815/1‑93 (MQ=255)

                tGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTgg                                                                         >  1:692247/1‑93 (MQ=255)

                tGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTgg                                                                         >  2:894945/1‑93 (MQ=255)

                  gaagaaTATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGaa                                                                       <  2:3930752/93‑1 (MQ=255)

                  gaagaaTATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGaa                                                                       >  2:1851488/1‑93 (MQ=255)

                  gaagaaTATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGaa                                                                       <  1:2143349/93‑1 (MQ=255)

                     gaaTATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGcc                                                                    >  2:3004968/1‑93 (MQ=255)

                     gaaTATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCAGCTTGGGCGCGAAAGTGCTGGAAGcc                                                                    >  2:1285092/1‑93 (MQ=255)

                      aaTATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCt                                                                   <  1:3589355/93‑1 (MQ=255)

                      aaTATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCt                                                                   >  2:2797299/1‑93 (MQ=255)

                       atatGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTg                                                                  >  1:3681530/1‑93 (MQ=255)

                       atatGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTg                                                                  >  1:1690688/1‑93 (MQ=255)

                        tatGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGc                                                                 <  1:330095/93‑1 (MQ=255)

                        tatGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGc                                                                 <  1:766781/93‑1 (MQ=255)

                           gCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCg                                                              <  2:2907570/93‑1 (MQ=255)

                            ccGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCgg                                                             >  1:2853040/1‑93 (MQ=255)

                             cGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGa                                                            <  1:2120986/93‑1 (MQ=255)

                                 ttCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGTCTGCCCGGCAGTc                                                        >  1:2009106/1‑93 (MQ=255)

                                 ttCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTc                                                        <  2:3458827/93‑1 (MQ=255)

                                 ttCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTc                                                        <  1:1969232/93‑1 (MQ=255)

                                 ttCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTc                                                        <  2:3305897/93‑1 (MQ=255)

                                   cGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCa                                                      <  2:81460/93‑1 (MQ=255)

                                    ctCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCAc                                                     <  1:2916790/92‑1 (MQ=255)

                                      cTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGa                                                   <  1:2165668/93‑1 (MQ=255)

                                       ttCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGAt                                                  >  2:1692458/1‑93 (MQ=255)

                                        tcatcaACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATc                                                 <  1:1547636/93‑1 (MQ=255)

                                         catcaACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCt                                                <  2:4353844/93‑1 (MQ=255)

                                          atcaACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTg                                               >  1:4248328/1‑93 (MQ=255)

                                           tcaACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGa                                              >  1:4394324/1‑93 (MQ=255)

                                           tcaACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGa                                              >  1:970068/1‑93 (MQ=255)

                                           tcaACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGa                                              <  2:10865/93‑1 (MQ=255)

                                           acaACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGa                                              <  1:883889/92‑1 (MQ=255)

                                             aaCATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGaaa                                            <  1:608168/93‑1 (MQ=255)

                                             aaCATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGaaa                                            >  1:621707/1‑93 (MQ=255)

                                              aCATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAAc                                           <  2:1739923/93‑1 (MQ=255)

                                              aCATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAAc                                           <  2:3730554/93‑1 (MQ=255)

                                              aCATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAAc                                           >  2:2069060/1‑93 (MQ=255)

                                               cATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGTCCGGAAGTCCACGATCTGAAAcg                                          >  2:3640436/1‑92 (MQ=255)

                                                aTGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTg                                         >  1:2582755/1‑93 (MQ=255)

                                                aTGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTg                                         >  2:4267891/1‑93 (MQ=255)

                                                aTGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTg                                         >  1:3863798/1‑93 (MQ=255)

                                                 tGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTgg                                        >  1:3968294/1‑93 (MQ=255)

                                                 tGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTgg                                        <  2:3558854/93‑1 (MQ=255)

                                                   gACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTggcg                                      <  2:1495970/93‑1 (MQ=255)

                                                   gACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTggcg                                      <  2:2760484/93‑1 (MQ=255)

                                                       ccAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGccc                                  >  1:2458998/1‑93 (MQ=255)

                                                       ccAACGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGccc                                  >  1:4105587/1‑93 (MQ=255)

                                                         aaCGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGc                                <  2:3243441/93‑1 (MQ=255)

                                                          aCGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCt                               <  2:4090038/93‑1 (MQ=255)

                                                           cGCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGGCTGACCGGAAGGCCACGATCTGAAACTGGCGGCCCGCtt                              >  2:1509612/1‑93 (MQ=255)

                                                            gCCACCAGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCttt                             <  1:1435592/93‑1 (MQ=255)

                                                             ccaccaGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTa                            >  2:2659147/1‑93 (MQ=255)

                                                             ccaccaGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTa                            >  1:3728514/1‑93 (MQ=255)

                                                               accaGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTacc                          <  2:705382/93‑1 (MQ=255)

                                                               accaGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTAAAACTGGCGGCCCGCTTTacc                          >  2:2847730/1‑93 (MQ=255)

                                                                ccaGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTacca                         <  2:1190522/93‑1 (MQ=255)

                                                                  aGCGTAGAAGATCAATTCCCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTaccacc                       <  1:4398411/93‑1 (MQ=255)

                                                                  aGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTaccacc                       <  2:2494642/93‑1 (MQ=255)

                                                                  aGCGTAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTaccacc                       <  1:4528743/93‑1 (MQ=255)

                                                                      tAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCa                   >  2:1489720/1‑93 (MQ=255)

                                                                      tAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCa                   <  2:2978533/93‑1 (MQ=255)

                                                                      tAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCa                   >  2:172664/1‑93 (MQ=255)

                                                                      tAGAAGATCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCa                   <  2:1127761/93‑1 (MQ=255)

                                                                       agaagaTCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCAc                  >  2:3388415/1‑93 (MQ=255)

                                                                        gaagaTCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACg                 >  2:3381467/1‑93 (MQ=255)

                                                                        gaagaTCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACg                 >  2:2519813/1‑93 (MQ=255)

                                                                        gaagaTCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACg                 >  1:1210479/1‑93 (MQ=255)

                                                                         aagaTCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCATTACCACCCGCACGc                >  1:1728/1‑93 (MQ=255)

                                                                          agaTCAATTACCCGGCTTGGGCGCGAAAGTGCTGGCAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGcc               >  1:4007561/1‑93 (MQ=255)

                                                                          agaTCAATTACCCGGCGTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGcc               <  1:362380/93‑1 (MQ=255)

                                                                            aTCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTg             <  2:594933/93‑1 (MQ=255)

                                                                             tCAATTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTTGCGGCCCGCTTTACCAACCGCACGGCTGc            >  2:2899009/1‑93 (MQ=255)

                                                                               aaTTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTGCCa          >  2:3006077/1‑93 (MQ=255)

                                                                                aTTACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTGCCaa         >  1:135731/1‑93 (MQ=255)

                                                                                 ttACCCGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTGCCAAc        >  2:2320805/1‑93 (MQ=255)

                                                                                     ccGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTGCCAACTGga    >  2:4077815/1‑93 (MQ=255)

                                                                                     ccGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTGCCAACTGga    >  1:4103900/1‑93 (MQ=255)

                                                                                     ccGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTGCCAACTGga    <  2:19779/93‑1 (MQ=255)

                                                                                     ccGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTGCCAACTGga    <  2:3113950/93‑1 (MQ=255)

                                                                                      cGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTGCCAACTggca   >  2:2693071/1‑91 (MQ=255)

                                                                                      cGGCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTGCCAACTGgaa   <  1:703475/93‑1 (MQ=255)

                                                                                       ggCTTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTGCCAACTGgaag  <  1:3437322/93‑1 (MQ=255)

CTGGTTCCGGTGCGTCTGGAAGAATATGCCGGATTCGTCTTCATCAACATGGACCCCAACGCCACCAGCGTAGAAGATCAATTACCCGGCCTGGGCGCGAAAGTGCTGGAAGCCTGCCCGGAAGTCCACGATCTGAAACTGGCGGCCCGCTTTACCACCCGCACGCCTGCCAACTGGAAG  >  NC_000913/1883109‑1883288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`