BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,434	G→A	A385T (GCT→ACT)	b1833 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,434	0	G	A	98.6%	486.5 / ‑5.7	142	A385T (GCT→ACT)	b1833	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (79/61); total (79/63)
Fisher's exact test for biased strand distribution p-value = 1.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

GATGGTGACCTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCGCTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTACTT  >  NC_000913/1915346‑1915523

gATGGTGACCTGGATTGGTCGCTGGTCGATTCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACttt                                                                                       >  2:923545/1‑93 (MQ=255)

gATGGTGACCTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACttt                                                                                       >  2:1477667/1‑93 (MQ=255)

  tGGTGACCTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTa                                                                                     >  2:3317702/1‑93 (MQ=255)

  tGGTGACCTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTa                                                                                     <  2:148671/93‑1 (MQ=255)

   ggTGACCTGGATTGGTCGCTAGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTAc                                                                                    <  1:4535494/93‑1 (MQ=255)

    gTGACCTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACt                                                                                   >  2:3543991/1‑93 (MQ=255)

     tGACCTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTa                                                                                  <  2:2422316/93‑1 (MQ=255)

     tGACCTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTa                                                                                  <  2:1897186/93‑1 (MQ=255)

     tGACCTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTa                                                                                  >  2:1687441/1‑93 (MQ=255)

      gACCTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTAt                                                                                 >  2:4675846/1‑93 (MQ=255)

       aCCTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATg                                                                                <  1:1162724/93‑1 (MQ=255)

        ccTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATgg                                                                               >  2:3494958/1‑93 (MQ=255)

         cTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATggg                                                                              <  1:4235238/93‑1 (MQ=255)

          tGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGa                                                                             <  1:1402576/93‑1 (MQ=255)

                  tCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGc                                                                     <  1:3477702/93‑1 (MQ=255)

                   cGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCg                                                                    >  1:1773106/1‑93 (MQ=255)

                    gCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGt                                                                   <  1:2378949/93‑1 (MQ=255)

                      tGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGttt                                                                 <  2:4182633/93‑1 (MQ=255)

                        gTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTa                                                               >  2:2359384/1‑93 (MQ=255)

                        gTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTa                                                               >  1:2345422/1‑93 (MQ=255)

                        gTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTa                                                               <  2:3607161/93‑1 (MQ=255)

                           gATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTAttt                                                            >  2:531320/1‑93 (MQ=255)

                            aTGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTc                                                           <  2:476393/93‑1 (MQ=255)

                             tGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCg                                                          >  1:3711725/1‑93 (MQ=255)

                             tGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCg                                                          >  1:3711724/1‑93 (MQ=255)

                             tGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCg                                                          >  2:3636016/1‑93 (MQ=255)

                              gCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCgg                                                         >  2:2714891/1‑93 (MQ=255)

                               cTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGgc                                                        <  2:49511/93‑1 (MQ=255)

                                 cGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGgcgc                                                      <  1:324518/93‑1 (MQ=255)

                                   aCCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAg                                                    >  2:3856023/1‑93 (MQ=255)

                                   aCCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAg                                                    >  2:833118/1‑93 (MQ=255)

                                    ccTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGc                                                   >  1:3391331/1‑93 (MQ=255)

                                    ccTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGc                                                   >  2:2698281/1‑93 (MQ=255)

                                    ccTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGc                                                   >  2:607947/1‑93 (MQ=255)

                                     cTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCg                                                  <  1:4475786/93‑1 (MQ=255)

                                      tGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCgg                                                 <  2:2818164/93‑1 (MQ=255)

                                        tttGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGTCGCAGGGGTa                                               >  2:1211184/1‑93 (MQ=255)

                                        tttGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTa                                               >  2:2050267/1‑93 (MQ=255)

                                         ttGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCGCTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTaa                                              <  1:212051/93‑1 (MQ=255)

                                         ttGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTaa                                              <  2:4658039/93‑1 (MQ=255)

                                           gTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAAtt                                            >  2:1305540/1‑93 (MQ=255)

                                           gTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAAtt                                            >  2:1109925/1‑93 (MQ=255)

                                            tCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAAttt                                           >  2:4297858/1‑93 (MQ=255)

                                              atatCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTg                                         >  1:830138/1‑93 (MQ=255)

                                               tatCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGa                                        >  2:6032/1‑93 (MQ=255)

                                               tatCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGa                                        >  1:1446253/1‑93 (MQ=255)

                                               tatCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGa                                        >  2:310173/1‑93 (MQ=255)

                                               tatCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGa                                        <  1:3290035/93‑1 (MQ=255)

                                               tatCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGGAATTTTGa                                        >  2:3996795/1‑93 (MQ=255)

                                                atCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGAc                                       >  1:106382/1‑93 (MQ=255)

                                                 tCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACt                                      >  1:236883/1‑93 (MQ=255)

                                                 tCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACt                                      >  1:3692491/1‑93 (MQ=255)

                                                  cTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGGTTTATTTCGGCGCAGCGGGAATTTTGACTa                                     >  1:4210184/1‑93 (MQ=255)

                                                   tttAACCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTAt                                    >  2:2537240/1‑93 (MQ=255)

                                                      aaCCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCt                                 <  2:2180766/93‑1 (MQ=255)

                                                       aCCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCtt                                >  1:1112636/1‑93 (MQ=255)

                                                       aCCATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCtt                                <  2:2228370/93‑1 (MQ=255)

                                                         cATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGc                              >  1:3882412/1‑93 (MQ=255)

                                                         cATGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGc                              <  1:810374/93‑1 (MQ=255)

                                                          aTGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCt                             >  2:3960447/1‑93 (MQ=255)

                                                          aTGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCt                             >  1:2853831/1‑93 (MQ=255)

                                                          aTGTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCt                             >  2:1497428/1‑93 (MQ=255)

                                                            gTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCtgt                           <  1:2425091/93‑1 (MQ=255)

                                                            gTCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCtgt                           <  2:1218626/93‑1 (MQ=255)

                                                             tCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCtgtg                          <  1:2071374/93‑1 (MQ=255)

                                                             tCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCtgtg                          <  1:2121868/93‑1 (MQ=255)

                                                             tCGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCtgtg                          >  1:2626107/1‑93 (MQ=255)

                                                              cGCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTgg                         <  1:2504633/93‑1 (MQ=255)

                                                               gCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGa                        >  2:535893/1‑93 (MQ=255)

                                                               gCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGa                        <  2:4147261/93‑1 (MQ=255)

                                                               gCTGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGa                        >  1:2667299/1‑93 (MQ=255)

                                                                 tGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGAGTATTCTTGCTGTGGAAt                      >  2:126702/1‑93 (MQ=255)

                                                                 tGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAAt                      <  1:4020203/93‑1 (MQ=255)

                                                                 tGATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACCATTCCTGCTGTGGAAt                      >  2:3640471/1‑93 (MQ=255)

                                                                  gATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATg                     >  2:46121/1‑93 (MQ=255)

                                                                  gATTAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATg                     >  1:11766/1‑93 (MQ=255)

                                                                    ttAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGc                   >  1:3052868/1‑93 (MQ=255)

                                                                    ttAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAACTTTGACTATTCTTGCTGTGGAATGGc                   >  2:2601426/1‑93 (MQ=255)

                                                                     tAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCt                  <  1:1022315/93‑1 (MQ=255)

                                                                     tAATCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCt                  >  1:4576120/1‑93 (MQ=255)

                                                                      aaTCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTg                 <  2:1469390/93‑1 (MQ=255)

                                                                        tCGCGATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGa               <  2:2900213/93‑1 (MQ=255)

                                                                           cgATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGTGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAg            <  1:3388029/93‑1 (MQ=255)

                                                                           cgATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAg            >  1:4058190/1‑93 (MQ=255)

                                                                            gATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGagagg           >  2:829854/1‑92 (MQ=255)

                                                                            gATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGc           >  2:3618555/1‑93 (MQ=255)

                                                                            gATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGc           >  1:3583748/1‑93 (MQ=255)

                                                                            gATCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGc           >  1:782578/1‑93 (MQ=255)

                                                                             aTCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACaagc          >  1:1990356/1‑90 (MQ=255)

                                                                             aTCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGcc          <  2:169053/93‑1 (MQ=255)

                                                                              tCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCg         <  2:3883113/93‑1 (MQ=255)

                                                                              tCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCg         >  2:841148/1‑93 (MQ=255)

                                                                              tCAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCg         <  1:3928088/93‑1 (MQ=255)

                                                                               cAGATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGc        >  2:1405123/1‑93 (MQ=255)

                                                                                 gATCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCtt      >  2:2200647/1‑93 (MQ=255)

                                                                                  aTCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTa     >  1:1280297/1‑93 (MQ=255)

                                                                                   tCCTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTAc    >  2:3276787/1‑93 (MQ=255)

                                                                                    ccTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTACt   >  1:2210795/1‑93 (MQ=255)

                                                                                     cTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTACtt  >  1:1333951/1‑93 (MQ=255)

                                                                                     cTCACTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTACtt  >  1:2191502/1‑93 (MQ=255)

GATGGTGACCTGGATTGGTCGCTGGTCGATGCTCGACCTGTTTGTCATATCTTTAACCATGTCGCTGATTAATCGCGATCAGATCCTCGCTTTTACTATGGGACCGGCTGCGTTTTATTTCGGCGCAGCGGTAATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTACTT  >  NC_000913/1915346‑1915523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`