Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,942,377 A→G M335T (ATG→ACG)  ruvB ← Holliday junction helicase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,942,3770AG92.6% 320.0 / 13.2 122M335T (ATG→ACG) ruvBHolliday junction helicase subunit A
Reads supporting (aligned to +/- strand):  ref base A (5/4);  new base G (57/56);  total (62/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.90e-01

Only 100 of 129 total aligned reads displayed.

CTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGCTGAAT  >  NC_000913/1942289‑1942465
                                                                                        |                                                                                        
ctGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTc                                                                                      <  2:1471574/93‑1 (MQ=255)
ctGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTc                                                                                      <  2:2654983/93‑1 (MQ=255)
ctGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTc                                                                                      <  2:749793/93‑1 (MQ=255)
  ggTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTg                                                                                    <  2:2587191/93‑1 (MQ=255)
  ggTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTg                                                                                    <  2:3317312/93‑1 (MQ=255)
   gTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTgg                                                                                   >  2:2056614/1‑93 (MQ=255)
   gTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTgg                                                                                   >  1:3714522/1‑93 (MQ=255)
    tAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTggc                                                                                  >  2:4421093/1‑93 (MQ=255)
      gTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTggcgg                                                                                >  1:488865/1‑93 (MQ=255)
      gTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTggcgg                                                                                >  1:4356276/1‑93 (MQ=255)
       tCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTggcggc                                                                               >  2:2003844/1‑93 (MQ=255)
         cTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGt                                                                             >  2:4034615/1‑93 (MQ=255)
         cTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGt                                                                             <  1:4127976/93‑1 (MQ=255)
           ggTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTa                                                                           <  2:4439783/93‑1 (MQ=255)
            gTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTAt                                                                          >  2:3106601/1‑93 (MQ=255)
             tAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATg                                                                         >  1:2661118/1‑93 (MQ=255)
             gaaGACCCGAAAAGCGGCGCATCCGGCATAATGCCAATGCCGGATGCGGCGCGAGCGACCAATCCGACTTTAGGCGTTTCTGGGGGGGTTTTg                                                                         >  2:1236987/2‑93 (MQ=255)
              aaGACGCGAACAGCGTCGCATCAGGCATATTTCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGc                                                                        >  1:3076292/1‑93 (MQ=255)
              aaGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGc                                                                        <  1:1001338/93‑1 (MQ=255)
              aaGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGc                                                                        >  1:3073918/1‑93 (MQ=255)
                gACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCa                                                                      >  1:4354223/1‑93 (MQ=255)
                  cgcgAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCaaa                                                                    <  1:3309489/93‑1 (MQ=255)
                   gcgAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAg                                                                   <  2:306017/93‑1 (MQ=255)
                   gcgAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAg                                                                   >  1:4134515/1‑93 (MQ=255)
                     gAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTg                                                                 >  1:1534590/1‑93 (MQ=255)
                     gAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGACGGCGTTATGCCAAAGTg                                                                 >  1:210492/1‑93 (MQ=255)
                      gaCAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGa                                                                <  2:3789384/92‑1 (MQ=255)
                      aaCAGCGTGGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGa                                                                >  2:3752760/1‑93 (MQ=255)
                      aaCAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGa                                                                <  1:2398864/93‑1 (MQ=255)
                       aCAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGAt                                                               <  2:1613846/93‑1 (MQ=255)
                        cAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGAtt                                                              >  2:4385336/1‑93 (MQ=255)
                         aGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTc                                                             <  2:358327/93‑1 (MQ=255)
                          gCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTcc                                                            >  2:2537056/1‑93 (MQ=255)
                          gCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTcc                                                            >  2:2594626/1‑93 (MQ=255)
                          gCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTcc                                                            >  2:1086455/1‑93 (MQ=255)
                           cGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCa                                                           <  1:3374985/93‑1 (MQ=255)
                            gTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCTGCGTTATGCCAAAGTGATTCCAc                                                          >  1:2294010/1‑93 (MQ=255)
                            gTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCAc                                                          <  1:3935274/93‑1 (MQ=255)
                                cATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGccc                                                      >  1:3320404/1‑93 (MQ=255)
                                cATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGccc                                                      <  1:3519257/93‑1 (MQ=255)
                                cATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGccc                                                      >  2:2207344/1‑93 (MQ=255)
                                cATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGccc                                                      >  2:4430221/1‑93 (MQ=255)
                                  tCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCcgc                                                    >  1:4103006/1‑93 (MQ=255)
                                  tCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCcgc                                                    >  1:1917221/1‑93 (MQ=255)
                                     ggCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGcgtc                                                 <  1:163146/93‑1 (MQ=255)
                                     ggCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGcgtc                                                 <  2:2790128/93‑1 (MQ=255)
                                       cATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCGACGCCCGcgtcgt                                               >  2:1608149/1‑93 (MQ=255)
                                       cATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGcgtcgt                                               >  1:1014889/1‑93 (MQ=255)
                                         tatTGCCATTGCGGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACTCCCGCGtcgtcg                                             <  2:4419865/93‑1 (MQ=255)
                                         tatTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGtcgtcg                                             >  1:3856670/1‑93 (MQ=255)
                                          atTGCAAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGc                                            >  1:1915948/1‑93 (MQ=255)
                                           ttGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGcc                                           >  2:1837536/1‑93 (MQ=255)
                                            tGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCa                                          <  2:2854828/93‑1 (MQ=255)
                                              ccAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATa                                        >  1:1160617/1‑93 (MQ=255)
                                               cAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATAc                                       >  2:2724826/1‑93 (MQ=255)
                                               cAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATAc                                       >  1:3472353/1‑93 (MQ=255)
                                                 gTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGc                                     >  1:2836180/1‑93 (MQ=255)
                                                 gTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGc                                     <  1:3004824/93‑1 (MQ=255)
                                                  tGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGcc                                    >  1:3438835/1‑93 (MQ=255)
                                                  tGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGcc                                    <  1:3292758/93‑1 (MQ=255)
                                                   gCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGccc                                   <  1:2729053/93‑1 (MQ=255)
                                                    ccGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCa                                  <  2:110162/93‑1 (MQ=255)
                                                     cGGATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCAc                                 <  2:3318534/93‑1 (MQ=255)
                                                      ggATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCAcg                                >  1:1058137/1‑93 (MQ=255)
                                                      ggATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCAcg                                >  1:4652934/1‑93 (MQ=255)
                                                       gATGCGGCGCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCAcgc                               >  1:1552336/1‑93 (MQ=255)
                                                       gATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCAcgc                               >  2:388506/1‑93 (MQ=255)
                                                          gcggcgCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGgt                            <  2:2486406/93‑1 (MQ=255)
                                                          gcggcgCGAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGgt                            <  2:4667879/93‑1 (MQ=255)
                                                              cgcgAGCGACCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTAc                        >  1:258461/1‑93 (MQ=255)
                                                                cgagcgaCCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCTCACGCGGTGTACGc                      <  2:2863679/93‑1 (MQ=255)
                                                                 gagcgaCCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTAGCCATACGCCCACGCGGTGTACGCt                     >  2:2234883/1‑93 (MQ=255)
                                                                     gaCCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCaa                 >  2:2142063/1‑93 (MQ=255)
                                                                     gaCCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCaa                 <  1:1183520/93‑1 (MQ=255)
                                                                      aCCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCaaa                >  1:800144/1‑93 (MQ=255)
                                                                      aCCAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCaaa                <  2:4031505/93‑1 (MQ=255)
                                                                      aCCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCaaa                >  1:4631292/1‑93 (MQ=255)
                                                                        cAATCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCaaaaa              <  2:3358906/93‑1 (MQ=255)
                                                                        cAATCCGACTTACGGCGTTTCTGGCGGCGTTACGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCaaaaa              <  2:3663869/93‑1 (MQ=255)
                                                                         aaTCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAg             <  1:262016/93‑1 (MQ=255)
                                                                         aaTCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAg             >  1:2228061/1‑93 (MQ=255)
                                                                          aTCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGc            <  2:1850510/93‑1 (MQ=255)
                                                                          aTCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGc            >  2:2452418/1‑93 (MQ=255)
                                                                          aTCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGc            >  2:1491917/1‑93 (MQ=255)
                                                                           tCCGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGcc           >  2:3008205/1‑93 (MQ=255)
                                                                            ccGACTTACGGCGTTTCTGGCGGCGTTATGGCAAAGTGATTTCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCt          <  1:4315566/93‑1 (MQ=255)
                                                                            ccGACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCt          <  1:139932/93‑1 (MQ=255)
                                                                              gACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTg        >  1:1443518/1‑93 (MQ=255)
                                                                              gACTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTg        <  2:4247221/93‑1 (MQ=255)
                                                                               aCTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGc       <  1:1659317/93‑1 (MQ=255)
                                                                               aCTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGc       <  2:739042/93‑1 (MQ=255)
                                                                                cTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGCt      >  1:1917158/1‑93 (MQ=255)
                                                                                cTTACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGCt      >  2:4401734/1‑93 (MQ=255)
                                                                                cTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAACAGCCTTGCt      >  1:2299904/1‑93 (MQ=255)
                                                                                  tACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGCTGa    <  1:4112342/93‑1 (MQ=255)
                                                                                  tACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGCTGa    <  1:2745849/93‑1 (MQ=255)
                                                                                  tACGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGCTGa    <  2:1995167/93‑1 (MQ=255)
                                                                                   aCGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGCTGaa   <  2:1999782/93‑1 (MQ=255)
                                                                                    cGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGCTGAAt  <  2:1947382/93‑1 (MQ=255)
                                                                                    cGGCGTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGCTGAAt  <  2:303657/93‑1 (MQ=255)
                                                                                        |                                                                                        
CTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGCTGAAT  >  NC_000913/1942289‑1942465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑