BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,959,708	C→T	S541S (AGC→AGT)	argS →	arginine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,959,708	0	C	T	99.2%	455.4 / ‑5.6	131	S541S (AGC→AGT)	argS	arginine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (75/55); total (75/56)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 135 total aligned reads displayed.

CCGTGAAGGCACGCCGCATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGCGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCTGGGTAT  >  NC_000913/1959618‑1959797

 cGTGAAGGCACGCCGCATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCa                                                                                        <  1:3042331/93‑1 (MQ=255)

  gTGAAGGCACGCCGCATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAg                                                                                       >  1:2345728/1‑93 (MQ=255)

  gTGAAGGCACGCCGCATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAg                                                                                       >  1:1890822/1‑93 (MQ=255)

  gTGAAGGCACGCCGCATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAg                                                                                       >  2:2897915/1‑93 (MQ=255)

   tGAAGGCACGCCGCATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGa                                                                                      >  2:1960017/1‑93 (MQ=255)

   tGAAGGCACGCCGCATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGa                                                                                      >  1:1392368/1‑93 (MQ=255)

     aaGGCACGCCGCATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGaaa                                                                                    >  2:707356/1‑93 (MQ=255)

      aGGCACGCCGCATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGaaac                                                                                   >  1:1495197/1‑92 (MQ=255)

       ggCACGCCGCATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAAc                                                                                  >  1:90192/1‑93 (MQ=255)

          aCGCCGCATGTAATGTGGGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACgaa                                                                               <  1:2598501/93‑1 (MQ=255)

           cgccgcATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAATgaag                                                                              >  1:795884/1‑93 (MQ=255)

            gccgcATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACgaaga                                                                             <  2:440484/93‑1 (MQ=255)

             ccgcATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACgaagaa                                                                            <  2:1103189/93‑1 (MQ=255)

              cgcATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGaagaag                                                                           <  2:4187300/93‑1 (MQ=255)

               gcATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGt                                                                          <  2:767710/93‑1 (MQ=255)

                cATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTg                                                                         >  2:24611/1‑93 (MQ=255)

                 aTGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGc                                                                        <  2:1162275/93‑1 (MQ=255)

                 aTGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGc                                                                        >  1:287936/1‑93 (MQ=255)

                  tGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCg                                                                       >  2:1434273/1‑93 (MQ=255)

                  tGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCg                                                                       >  2:4522771/1‑93 (MQ=255)

                  tGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCg                                                                       >  1:3808337/1‑93 (MQ=255)

                  tGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCg                                                                       >  1:3030431/1‑93 (MQ=255)

                   gTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGt                                                                      >  2:2919679/1‑93 (MQ=255)

                   gTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGAGCGt                                                                      >  1:4263418/1‑93 (MQ=255)

                     aaTGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTaa                                                                    <  2:1311005/93‑1 (MQ=255)

                     aaTGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTaa                                                                    >  2:4412307/1‑93 (MQ=255)

                     aaTGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTaa                                                                    >  1:1210403/1‑93 (MQ=255)

                     aaTGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTaa                                                                    <  1:3000004/93‑1 (MQ=255)

                      aTGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAAc                                                                   <  1:3298558/93‑1 (MQ=255)

                       tgtgtgCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACa                                                                  <  1:58965/93‑1 (MQ=255)

                       tgtgtgCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACa                                                                  <  2:2952229/93‑1 (MQ=255)

                       tgtgtgCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACa                                                                  >  1:2455496/1‑93 (MQ=255)

                          gtgCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGcc                                                               <  1:1846655/93‑1 (MQ=255)

                           tgCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGACg                                                              >  1:3266338/1‑93 (MQ=255)

                             cTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTc                                                            <  2:594847/93‑1 (MQ=255)

                              ttACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCt                                                           >  2:1092049/1‑93 (MQ=255)

                              ttACCTGTACGATCTGGCCGGTCTGTTCGCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCt                                                           <  1:2039669/93‑1 (MQ=255)

                               tACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTa                                                          <  2:1838615/93‑1 (MQ=255)

                                 ccTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTaaa                                                        >  1:3647963/1‑93 (MQ=255)

                                  cTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTaaaa                                                       <  1:3581887/93‑1 (MQ=255)

                                   tGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAAc                                                      <  1:1670273/93‑1 (MQ=255)

                                   tGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAAc                                                      >  1:3884892/1‑93 (MQ=255)

                                   cttACTATTTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAAc                                                      <  1:2559901/91‑1 (MQ=255)

                                      aCGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTgg                                                   >  1:2222786/1‑93 (MQ=255)

                                        gATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGca                                                 >  1:4042270/1‑93 (MQ=255)

                                        gATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGca                                                 >  2:380380/1‑93 (MQ=255)

                                         aTCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGcac                                                <  1:1087475/93‑1 (MQ=255)

                                         aTCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGcac                                                <  2:1925104/93‑1 (MQ=255)

                                         aTCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGcac                                                >  1:1275864/1‑93 (MQ=255)

                                         aTCTGGCCGGTCTCTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGCcac                                                <  2:345590/93‑1 (MQ=255)

                                          tCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGcaca                                               >  1:2224375/1‑93 (MQ=255)

                                            tGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAAc                                             <  2:1962234/93‑1 (MQ=255)

                                               ccGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGa                                          <  2:3584085/93‑1 (MQ=255)

                                               ccGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGa                                          >  1:951380/1‑93 (MQ=255)

                                                 ggTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACg                                        <  1:1022219/93‑1 (MQ=255)

                                                  gTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACgg                                       >  1:4119108/1‑93 (MQ=255)

                                                  gTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACgg                                       >  2:3005457/1‑93 (MQ=255)

                                                  gTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACgg                                       >  2:2056477/1‑93 (MQ=255)

                                                   tCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGc                                      >  2:2257288/1‑93 (MQ=255)

                                                      gTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGaa                                   >  1:2697488/1‑93 (MQ=255)

                                                      gTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGaa                                   <  2:4661144/93‑1 (MQ=255)

                                                      gTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGaa                                   <  2:240438/93‑1 (MQ=255)

                                                      gTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGaa                                   <  2:409157/93‑1 (MQ=255)

                                                       ttCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGTCGAAg                                  >  1:2927475/1‑93 (MQ=255)

                                                       ttCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAg                                  >  1:1035127/1‑93 (MQ=255)

                                                         ctctGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGAc                                >  2:3800470/1‑93 (MQ=255)

                                                          tctGGCTTCTACGAGCACTGCCCGATCCTCCGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGaag                               >  1:537539/1‑91 (MQ=255)

                                                          tctGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACg                               <  1:656713/93‑1 (MQ=255)

                                                          tctGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAAAGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGGAGACg                               >  2:4050121/1‑93 (MQ=255)

                                                           ctGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGc                              <  2:2808804/93‑1 (MQ=255)

                                                            tGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCt                             >  1:1605465/1‑93 (MQ=255)

                                                            tGGCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCt                             <  1:1257868/93‑1 (MQ=255)

                                                             ggCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTg                            >  1:4029169/1‑93 (MQ=255)

                                                              gCTTCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGa                           >  2:1306776/1‑93 (MQ=255)

                                                                 tCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGc                        <  2:2492161/93‑1 (MQ=255)

                                                                 tCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGc                        >  2:1559777/1‑93 (MQ=255)

                                                                 tCTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGc                        >  1:3199601/1‑93 (MQ=255)

                                                                  cTACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCt                       <  2:3413323/93‑1 (MQ=255)

                                                                   tACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAATCTGGCACAACTGACGGCGAAGACGCTGAAGCTg                      <  1:4599055/93‑1 (MQ=255)

                                                                   tACGAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTg                      >  1:1869704/1‑93 (MQ=255)

                                                                      gAGCACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGt                   >  2:263090/1‑93 (MQ=255)

                                                                      gAGCACTGCCCGATCCTCAGCGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGACACAACTGACGGCGAAGACGCTGAAGCTGGGt                   <  1:3342063/93‑1 (MQ=255)

                                                                         cACTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTg                <  1:2409363/93‑1 (MQ=255)

                                                                          aCTGCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTgg               >  1:2702231/1‑93 (MQ=255)

                                                                             gCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATa            >  1:1672815/1‑93 (MQ=255)

                                                                             gCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATa            <  1:3824323/93‑1 (MQ=255)

                                                                             gCCCGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATa            <  1:3758496/93‑1 (MQ=255)

                                                                              cccGATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATAc           <  2:3991356/93‑1 (MQ=255)

                                                                                 gATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCt        >  2:1570310/1‑93 (MQ=255)

                                                                                 gATCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCt        <  2:1757665/93‑1 (MQ=255)

                                                                                  aTCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCTg       >  1:2642042/1‑93 (MQ=255)

                                                                                   tcgtCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAATCTGGGTCTGGATACGCTgg      <  1:1619922/90‑1 (MQ=255)

                                                                                   tCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCTgg      >  1:4403585/1‑93 (MQ=255)

                                                                                   tCCTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCTgg      >  2:2999073/1‑93 (MQ=255)

                                                                                    ccTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCTggg     >  2:3699523/1‑93 (MQ=255)

                                                                                    ccTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCTggg     <  1:3830329/93‑1 (MQ=255)

                                                                                     cTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCTGGGt    >  2:2698607/1‑93 (MQ=255)

                                                                                     cTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCTGGGt    >  2:2698604/1‑93 (MQ=255)

                                                                                     cTCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCTGGGt    >  1:3356433/1‑93 (MQ=255)

                                                                                      tCAGTGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCTGGGTa   >  2:1804831/1‑93 (MQ=255)

CCGTGAAGGCACGCCGCATGTAATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTCAGCGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAGACGCTGAAGCTGGGTCTGGATACGCTGGGTAT  >  NC_000913/1959618‑1959797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`