BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	198,418	A→G	D164G (GAC→GGC)	yaeT →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	198,418	0	A	G	99.5%	670.7 / NA	209	D164G (GAC→GGC)	yaeT	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (99/109); minor base T (0/1); total (99/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

AGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGA  >  NC_000913/198328‑198507

agaAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGcc                                                                                         <  1:82362/93‑1 (MQ=255)

 gaAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCt                                                                                        >  1:415934/1‑93 (MQ=255)

 gaAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGGTGGCCt                                                                                        >  2:4049412/1‑93 (MQ=255)

    aGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTaaa                                                                                     >  2:1961070/1‑93 (MQ=255)

       tCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACt                                                                                  <  1:1155226/93‑1 (MQ=255)

       tCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACt                                                                                  <  2:1264177/93‑1 (MQ=255)

         tGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTgg                                                                                >  1:2817088/1‑93 (MQ=255)

         tGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTgg                                                                                <  2:3738794/93‑1 (MQ=255)

         tGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTgg                                                                                <  2:3546986/93‑1 (MQ=255)

         tGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTgg                                                                                <  2:3486076/93‑1 (MQ=255)

         tGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTgg                                                                                >  1:180234/1‑93 (MQ=255)

          ggAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGgt                                                                               <  1:3110700/93‑1 (MQ=255)

          ggAAGACTTCTACTACAGCGTCAGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGgt                                                                               <  1:4243256/93‑1 (MQ=255)

             aGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGtt                                                                            <  1:1916389/93‑1 (MQ=255)

             aGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGtt                                                                            <  2:639338/93‑1 (MQ=255)

              gACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTc                                                                           >  2:1364783/1‑93 (MQ=255)

              gACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTc                                                                           <  1:2286381/93‑1 (MQ=255)

                cTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTccg                                                                         >  2:559712/1‑92 (MQ=255)

                cTTCTACTACAGCGTCGGTAAATATAGCGCAAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCa                                                                         <  1:4139939/93‑1 (MQ=255)

                  tCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAgg                                                                       >  1:1212109/1‑93 (MQ=255)

                    tactacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGaa                                                                     <  1:1380505/93‑1 (MQ=255)

                     actacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAg                                                                    <  2:3173226/93‑1 (MQ=255)

                     actacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAg                                                                    >  2:1714418/1‑93 (MQ=255)

                     actacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAg                                                                    <  1:649897/93‑1 (MQ=255)

                       tacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgt                                                                  >  2:3399751/1‑93 (MQ=255)

                       tacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgt                                                                  >  1:3342780/1‑93 (MQ=255)

                       tacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgt                                                                  <  2:911098/93‑1 (MQ=255)

                          aGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgtgtg                                                               >  2:1592387/1‑93 (MQ=255)

                           gCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgtgtgt                                                              >  1:1650071/1‑93 (MQ=255)

                              tCGGTAAATATAGCGCCAGCGTAAACGCTGTCGTGACCCCGCTGCCGCGCCACCGTGTTGGACTAAAACTGGTGTTCCAGGAAGGTGTGTCAg                                                           >  2:526139/1‑93 (MQ=255)

                              tCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAg                                                           >  1:185888/1‑93 (MQ=255)

                               cGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGc                                                          <  2:3887394/93‑1 (MQ=255)

                               cGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGc                                                          <  1:559879/93‑1 (MQ=255)

                               cGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGc                                                          <  1:4081737/93‑1 (MQ=255)

                                ggTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCt                                                         <  1:582353/93‑1 (MQ=255)

                                ggTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCt                                                         >  1:4562070/1‑93 (MQ=255)

                                  tAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGa                                                       >  1:103749/1‑93 (MQ=255)

                                  tAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGa                                                       <  2:3857085/93‑1 (MQ=255)

                                   aaaTATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGaa                                                      <  1:4026049/93‑1 (MQ=255)

                                    aaTATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGaaa                                                     <  1:1688464/93‑1 (MQ=255)

                                    aaTATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGaaa                                                     >  1:4304734/1‑93 (MQ=255)

                                    aaTATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGaaa                                                     >  1:4600606/1‑93 (MQ=255)

                                     atatAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAAt                                                    <  1:454017/93‑1 (MQ=255)

                                      tataGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATc                                                   >  1:358323/1‑93 (MQ=255)

                                      tataGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATc                                                   <  2:4106104/93‑1 (MQ=255)

                                       ataGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATcc                                                  >  2:342749/1‑93 (MQ=255)

                                       ataGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATcc                                                  <  1:1450341/93‑1 (MQ=255)

                                       ataGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATcc                                                  >  2:2126021/1‑93 (MQ=255)

                                        taGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCa                                                 <  1:802517/93‑1 (MQ=255)

                                        taGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCa                                                 <  1:220007/93‑1 (MQ=255)

                                          gcgcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGc                                               >  2:2805433/1‑93 (MQ=255)

                                          gcgcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGc                                               <  2:844282/93‑1 (MQ=255)

                                          gcgcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGCGTTGGCCTAAAACTGGGGTTCCAGGAAGGGGTGTCAGCTGAAAGCCAGc                                               >  1:1308931/1‑93 (MQ=255)

                                           cgcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCa                                              <  2:4229191/93‑1 (MQ=255)

                                            cccAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCaa                                             <  2:3070255/92‑1 (MQ=255)

                                             ccAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTGAGCTGAAATCCAGCaaa                                            >  1:2240040/1‑93 (MQ=255)

                                             ccAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCaaa                                            <  2:2509999/93‑1 (MQ=255)

                                             ccAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCaaa                                            >  2:1775886/1‑93 (MQ=255)

                                             ccAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCaaa                                            <  1:1480961/93‑1 (MQ=255)

                                              cAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAAt                                           <  1:74978/93‑1 (MQ=255)

                                               aGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAAtt                                          <  1:2622435/93‑1 (MQ=255)

                                                gCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTa                                         <  2:2477629/93‑1 (MQ=255)

                                                  gTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTaaa                                       >  2:3593648/1‑92 (MQ=255)

                                                  gTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGGGTCAGCTGAAATCCAGCAAATTAAc                                       >  2:1017479/1‑93 (MQ=255)

                                                   tAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACa                                      <  1:1285669/93‑1 (MQ=255)

                                                   tAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGGGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTacct                                      >  1:3515079/1‑90 (MQ=255)

                                                    aaaaGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGAGTCAGCTGCAATCCAGCCAATTAACAt                                     >  1:2073593/1‑93 (MQ=255)

                                                       aGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttgt                                  <  1:2416047/93‑1 (MQ=255)

                                                       aGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttgt                                  >  2:4521134/1‑93 (MQ=255)

                                                       aGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttgt                                  >  2:2614865/1‑93 (MQ=255)

                                                        gCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttgtt                                 <  2:2971644/93‑1 (MQ=255)

                                                        gCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttgtt                                 <  1:3190624/93‑1 (MQ=255)

                                                        gCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttgtt                                 >  1:3125129/1‑93 (MQ=255)

                                                         cTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttgttg                                >  2:2725594/1‑93 (MQ=255)

                                                         cTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttgttg                                <  2:3248107/93‑1 (MQ=255)

                                                           gTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGt                              >  2:2176471/1‑93 (MQ=255)

                                                           gTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGt                              <  1:3844330/93‑1 (MQ=255)

                                                            tCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTa                             >  2:2289745/1‑93 (MQ=255)

                                                                 aCCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCAt                        >  2:1124716/1‑93 (MQ=255)

                                                                 aCCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCAt                        >  2:119763/1‑93 (MQ=255)

                                                                  ccccGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATg                       <  1:3425838/93‑1 (MQ=255)

                                                                   cccGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGc                      <  1:1781398/93‑1 (MQ=255)

                                                                    ccGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCt                     >  2:1217954/1‑93 (MQ=255)

                                                                    ccGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCt                     >  1:1137220/1‑93 (MQ=255)

                                                                    ccGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCt                     >  2:4672712/1‑93 (MQ=255)

                                                                     cGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCtt                    <  2:3765873/93‑1 (MQ=255)

                                                                      gCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCttt                   >  1:1184227/1‑93 (MQ=255)

                                                                            gcgcAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTcaccac             >  2:1275951/1‑93 (MQ=255)

                                                                            gcgcAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTcaccac             <  1:249370/93‑1 (MQ=255)

                                                                               cAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcga          <  1:4375534/93‑1 (MQ=255)

                                                                                aaCCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcgac         <  2:1492956/93‑1 (MQ=255)

                                                                                 aCCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcgacg        <  1:2649799/93‑1 (MQ=255)

                                                                                 aCCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcgacg        >  1:3397093/1‑93 (MQ=255)

                                                                                  ccGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcgacga       <  1:2785050/93‑1 (MQ=255)

                                                                                  ccGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcgacga       >  1:3626328/1‑93 (MQ=255)

                                                                                    gtgtTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAAc     <  2:1617267/93‑1 (MQ=255)

                                                                                    gtgtTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAAc     >  1:3263373/1‑93 (MQ=255)

                                                                                     tgtTGGCGTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACt    <  2:1829758/93‑1 (MQ=255)

                                                                                     tgtTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACt    >  1:3736704/1‑93 (MQ=255)

                                                                                     tgtTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACt    >  2:1187531/1‑93 (MQ=255)

AGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGA  >  NC_000913/198328‑198507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`