BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,980,319	A→G	H31H (CAT→CAC)	otsB ←	trehalose‑6‑phosphate phophatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,980,319	0	A	G	100.0%	480.7 / NA	153	H31H (CAT→CAC)	otsB	trehalose‑6‑phosphate phophatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (82/71); total (82/71)

Only 100 of 161 total aligned reads displayed.

CCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGT  >  NC_000913/1980229‑1980408

cccTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTg                                                                                         >  2:3728002/1‑93 (MQ=255)

 ccTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGc                                                                                        <  1:525198/93‑1 (MQ=255)

 ccTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGc                                                                                        >  1:930891/1‑93 (MQ=255)

  cTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCg                                                                                       >  1:1154192/1‑93 (MQ=255)

  cTGATATCAATGCCAATGCACCATCACTTGCGGCTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCg                                                                                       >  1:856629/1‑93 (MQ=255)

   tGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCgg                                                                                      >  2:733895/1‑93 (MQ=255)

     atatCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCCGGGTGCGGtt                                                                                    >  2:2516646/1‑93 (MQ=255)

      tatCAATGCCAATGCACCATCGCTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGttt                                                                                   <  1:3224577/93‑1 (MQ=255)

      aatCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGttt                                                                                   <  1:4144812/92‑1 (MQ=255)

       atCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTg                                                                                  >  2:291371/1‑93 (MQ=255)

        tCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTgt                                                                                 >  1:2985030/1‑92 (MQ=255)

        tCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGa                                                                                 <  2:2394096/93‑1 (MQ=255)

        tCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGa                                                                                 >  2:3798727/1‑93 (MQ=255)

        tCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGa                                                                                 >  1:2167075/1‑93 (MQ=255)

         caatgccaatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGAt                                                                                >  1:2585163/1‑93 (MQ=255)

          aatgccaatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGAtt                                                                               <  2:4205629/93‑1 (MQ=255)

          aatgccaatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGAtt                                                                               <  1:2423163/93‑1 (MQ=255)

          aatgccaatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGAtt                                                                               >  1:3991048/1‑93 (MQ=255)

           atgccaatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGAttt                                                                              >  1:947240/1‑93 (MQ=255)

           atgccaatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGAttt                                                                              >  1:106769/1‑93 (MQ=255)

            tgccaatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTc                                                                             <  2:1777030/93‑1 (MQ=255)

            tgccaatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTc                                                                             <  1:2478211/93‑1 (MQ=255)

            tgccaatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTc                                                                             >  2:1572985/1‑93 (MQ=255)

            tgccaatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTc                                                                             >  1:3606302/1‑93 (MQ=255)

            tgccaatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTc                                                                             >  1:3313344/1‑93 (MQ=255)

               caatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGc                                                                          >  2:1544570/1‑93 (MQ=255)

                aatgcACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGcc                                                                         >  1:3354077/1‑93 (MQ=255)

                    cACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCg                                                                     >  2:2791403/1‑93 (MQ=255)

                     aCCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGt                                                                    >  1:619779/1‑93 (MQ=255)

                     aCCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGt                                                                    >  1:2671191/1‑93 (MQ=255)

                     aCCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGt                                                                    >  1:1676430/1‑93 (MQ=255)

                     aCCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGGGCGGTTTGATTTTCGCCAGCGt                                                                    >  1:3481250/1‑93 (MQ=255)

                      ccATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGtt                                                                   >  2:2194985/1‑93 (MQ=255)

                        aTCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTcc                                                                 <  2:2634537/93‑1 (MQ=255)

                        aTCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTcc                                                                 <  1:3344207/93‑1 (MQ=255)

                         tCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGCTTGATTTCCGCCAGCGTTCCa                                                                >  1:3919517/1‑93 (MQ=255)

                         tCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAAGATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCa                                                                <  1:4519589/93‑1 (MQ=255)

                           aCTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATc                                                              >  2:2613894/1‑93 (MQ=255)

                           aCTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATc                                                              >  1:3543400/1‑93 (MQ=255)

                            cTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCa                                                             >  2:1370647/1‑93 (MQ=255)

                             ttGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCaa                                                            <  1:1765433/93‑1 (MQ=255)

                             ttGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCaa                                                            <  2:539320/93‑1 (MQ=255)

                             ttGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCaa                                                            <  2:3322942/93‑1 (MQ=255)

                              tGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAg                                                           <  2:206318/93‑1 (MQ=255)

                                cGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGAt                                                         <  2:2211914/93‑1 (MQ=255)

                                   ttGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCaa                                                      <  2:1201672/93‑1 (MQ=255)

                                   ttGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCaa                                                      <  1:534382/93‑1 (MQ=255)

                                    tGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCaaa                                                     >  2:1169599/1‑93 (MQ=255)

                                     gCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCaaaa                                                    <  1:1861231/93‑1 (MQ=255)

                                      ccAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCaaaaa                                                   >  1:75926/1‑93 (MQ=255)

                                       cAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCaaaaaa                                                  >  2:3247869/1‑93 (MQ=255)

                                        aGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAAc                                                 <  2:396185/93‑1 (MQ=255)

                                        aGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAAc                                                 >  2:3331352/1‑93 (MQ=255)

                                         gTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAAcc                                                >  2:1923710/1‑93 (MQ=255)

                                         gTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAAcc                                                >  2:910672/1‑93 (MQ=255)

                                         gTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAAcc                                                >  2:49233/1‑93 (MQ=255)

                                             cTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGc                                            <  1:1876868/93‑1 (MQ=255)

                                             cTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGc                                            >  1:594901/1‑93 (MQ=255)

                                              tGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCa                                           <  2:1750382/93‑1 (MQ=255)

                                              tGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCa                                           <  2:3491771/93‑1 (MQ=255)

                                               gTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCat                                          >  2:4326940/1‑93 (MQ=255)

                                               gTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCat                                          <  2:3558965/93‑1 (MQ=255)

                                               gTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCat                                          >  2:3949764/1‑93 (MQ=255)

                                                tAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCata                                         >  1:2917890/1‑93 (MQ=255)

                                                tAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCata                                         <  1:213091/93‑1 (MQ=255)

                                                 aGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCatat                                        <  2:4182565/93‑1 (MQ=255)

                                                  gTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATAtt                                       <  1:1669449/93‑1 (MQ=255)

                                                   tCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCTGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATAttt                                      >  1:1783571/1‑93 (MQ=255)

                                                    ccTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTc                                     <  2:399180/93‑1 (MQ=255)

                                                     cTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTcg                                    <  1:4337690/93‑1 (MQ=255)

                                                      ttGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTcgc                                   >  2:4623929/1‑93 (MQ=255)

                                                       tGCAGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTcgcg                                  <  1:1401749/93‑1 (MQ=255)

                                                          aGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGAt                               >  1:4229171/1‑93 (MQ=255)

                                                          aGAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGAt                               <  1:1039898/93‑1 (MQ=255)

                                                           gAATATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATa                              >  2:845336/1‑93 (MQ=255)

                                                            aaTATTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAg                             >  1:3679721/1‑93 (MQ=255)

                                                             atatTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGt                            >  1:3137778/1‑93 (MQ=255)

                                                             atatTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGt                            <  1:1190022/93‑1 (MQ=255)

                                                               atTGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTc                          <  1:2813090/93‑1 (MQ=255)

                                                                ttGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCa                         >  2:4475864/1‑93 (MQ=255)

                                                                 tGTCAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAg                        <  2:2322173/93‑1 (MQ=255)

                                                                  gTCAGGCACGACGACCTGATCGGGGTGCGGTTCGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACTAGGCATATTTCGCGGATAGTTCAgg                       >  1:2065809/1‑93 (MQ=255)

                                                                    cAGGCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAgggg                     >  1:3038146/1‑93 (MQ=255)

                                                                      ggCACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAggggct                   >  2:1887162/1‑91 (MQ=255)

                                                                        cACGACGACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTc                 <  1:650020/93‑1 (MQ=255)

                                                                         acgacgACCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCg                >  1:2919834/1‑93 (MQ=255)

                                                                          cgacgaCCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCgg               <  1:767826/93‑1 (MQ=255)

                                                                              gacCTGATCGGGGTGCGTTTTGATTTCCGCCAGCGTTACATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTaa           <  2:3595384/93‑1 (MQ=255)

                                                                               acCTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAAc          >  2:4445999/1‑93 (MQ=255)

                                                                                 cTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAACCCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACgg        <  2:1916947/93‑1 (MQ=255)

                                                                                 cTGATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACgg        <  1:461783/93‑1 (MQ=255)

                                                                                 cTGATCGGGGCGCGGATTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGATCAGGGGTTTCGGTTAACgg        >  2:3037252/1‑93 (MQ=255)

                                                                                  tGATCGGGGGGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTCACGGt       >  2:3201429/1‑93 (MQ=255)

                                                                                   gATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGtt      >  2:2560532/1‑93 (MQ=255)

                                                                                   gATCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGtt      >  1:4244927/1‑93 (MQ=255)

                                                                                    ataggggTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTc     <  1:345250/90‑1 (MQ=255)

                                                                                    aTCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTc     <  2:3130539/93‑1 (MQ=255)

                                                                                    aTCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTc     <  2:2407242/93‑1 (MQ=255)

                                                                                    aTCGGGGTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTc     <  1:1964207/93‑1 (MQ=255)

                                                                                       ggggTGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGt  >  1:3018934/1‑93 (MQ=255)

CCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGT  >  NC_000913/1980229‑1980408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`