Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 206,315 T→C V397A (GTC→GCC)  dnaE → DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913206,3150TC96.0% 504.8 / 7.8 179V397A (GTC→GCC) dnaEDNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (2/5);  new base C (77/93);  total (81/98)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01

Only 100 of 194 total aligned reads displayed.

GTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGTCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCGG  >  NC_000913/206225‑206404
                                                                                          |                                                                                         
gTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGcct                                                                                         <  1:4097355/93‑1 (MQ=255)
 ttCACTGGTGGCCTACGCGCTGAAAATCTCCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGcctc                                                                                        >  1:1402554/1‑93 (MQ=255)
 ttCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGcctc                                                                                        >  1:3531830/1‑93 (MQ=255)
   cACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCa                                                                                      <  2:3920907/93‑1 (MQ=255)
     cTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATg                                                                                    >  2:2539569/1‑93 (MQ=255)
      tggtggCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGc                                                                                   >  2:719788/1‑93 (MQ=255)
      tggtggCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGACTCCATGc                                                                                   >  1:452266/1‑93 (MQ=255)
        gtggCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCt                                                                                 <  2:4415145/93‑1 (MQ=255)
          ggCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGa                                                                               <  2:4216704/93‑1 (MQ=255)
           gCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGAc                                                                              <  1:1181998/93‑1 (MQ=255)
           gCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGACTCCATGCCTGAc                                                                              >  2:3694538/1‑93 (MQ=255)
              tACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTc                                                                           <  2:4511064/93‑1 (MQ=255)
              tACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTc                                                                           <  2:3824704/93‑1 (MQ=255)
              tACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTc                                                                           >  2:3762742/1‑93 (MQ=255)
              tACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTc                                                                           >  1:4452025/1‑93 (MQ=255)
              tACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTc                                                                           >  2:3555751/1‑93 (MQ=255)
              tACGCGCTAAAAAACACCGGCCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTc                                                                           <  1:801067/93‑1 (MQ=255)
                   gcTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGt                                                                      <  2:1166918/93‑1 (MQ=255)
                   gcTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGt                                                                      >  1:3320048/1‑93 (MQ=255)
                    cTGAAAATCACCGCCCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGtt                                                                     <  2:3350567/93‑1 (MQ=255)
                    cTGAAAATCACCGACCTCGATCAGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGtt                                                                     >  1:2507861/1‑93 (MQ=255)
                        aaaCCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTTCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACt                                                                 <  1:4271545/93‑1 (MQ=255)
                           tCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCt                                                              >  1:2783502/1‑93 (MQ=255)
                               cGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTAt                                                          <  2:2693769/93‑1 (MQ=255)
                                gACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATg                                                         <  1:1056163/93‑1 (MQ=255)
                                gACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATg                                                         <  1:1646227/93‑1 (MQ=255)
                                 aCCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGCCTTCTGTATgg                                                        >  1:3022263/1‑93 (MQ=255)
                                 aCCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATgg                                                        >  1:4397243/1‑93 (MQ=255)
                                    tCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGgaga                                                     >  1:3136338/1‑93 (MQ=255)
                                    tCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGgaga                                                     >  2:1398555/1‑93 (MQ=255)
                                     cGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGaa                                                    <  1:2991667/93‑1 (MQ=255)
                                     cGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGaa                                                    >  2:2001603/1‑93 (MQ=255)
                                      gATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGaaa                                                   <  2:1385235/93‑1 (MQ=255)
                                        tCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAAcg                                                 >  1:3862218/1‑93 (MQ=255)
                                        tCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAAcg                                                 >  1:2259701/1‑93 (MQ=255)
                                         ccGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGTCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAAcgc                                                >  2:2251877/1‑93 (MQ=255)
                                         ccGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAAcgc                                                <  1:2395329/93‑1 (MQ=255)
                                         ccGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAAcgc                                                <  2:133732/93‑1 (MQ=255)
                                           gCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGa                                              >  1:2347562/1‑93 (MQ=255)
                                           gCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGa                                              <  2:3269327/93‑1 (MQ=255)
                                            cTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGAt                                             >  2:569372/1‑93 (MQ=255)
                                            cTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTACTGTATGGAGTAACGCGAt                                             <  2:4545039/93‑1 (MQ=255)
                                             tGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATc                                            >  2:1762303/1‑93 (MQ=255)
                                             tGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATc                                            >  1:3362582/1‑93 (MQ=255)
                                              ggAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCa                                           <  2:1740076/93‑1 (MQ=255)
                                              ggAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCa                                           <  1:4169546/93‑1 (MQ=255)
                                              ggAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCa                                           <  1:1357112/93‑1 (MQ=255)
                                               gAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAg                                          <  2:230972/93‑1 (MQ=255)
                                                aaTTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAgg                                         >  1:3249645/1‑93 (MQ=255)
                                                 aTTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGt                                        <  2:1966567/93‑1 (MQ=255)
                                                 aTTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGt                                        >  2:977471/1‑93 (MQ=255)
                                                  tttGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGtt                                       >  1:2856461/1‑93 (MQ=255)
                                                  tttGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTACTGTATGGAGAAACGCGATCAGGtt                                       <  1:403316/93‑1 (MQ=255)
                                                   ttGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTa                                      <  2:1783689/93‑1 (MQ=255)
                                                    tGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTAt                                     <  1:2599932/93‑1 (MQ=255)
                                                    tGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTAt                                     <  2:2511176/93‑1 (MQ=255)
                                                     gACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATc                                    >  1:1123068/1‑93 (MQ=255)
                                                     gACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATc                                    <  2:3197856/93‑1 (MQ=255)
                                                      gccTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCg                                   <  1:4356213/92‑1 (MQ=255)
                                                       ccTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGa                                  <  2:905385/93‑1 (MQ=255)
                                                       ccTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGGTATCGa                                  >  2:4383431/1‑93 (MQ=255)
                                                        gtgctgTTCGAACGTTTCCTTAACCCGGAATCTGCCTCCATGCCTGACTTCGACGTTGACTTATGTATGGAGAAACGTGATCAGGTTATTGAg                                 >  2:2738096/2‑93 (MQ=255)
                                                        ctgctgTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGGAACGCGATCAGGTTATCGAg                                 >  2:2852877/1‑93 (MQ=255)
                                                        ctgctgTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAg                                 >  2:3863909/1‑93 (MQ=255)
                                                         tgctgTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGc                                >  1:1702705/1‑93 (MQ=255)
                                                         tgctgTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGc                                <  1:507161/93‑1 (MQ=255)
                                                         tgctgTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGc                                <  1:3810923/93‑1 (MQ=255)
                                                          gctgTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCa                               <  2:3144410/93‑1 (MQ=255)
                                                            tgTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACg                             >  1:2166283/1‑93 (MQ=255)
                                                             gTTCGAACGTTTCCTTAACCCGGAACGTGTCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGt                            <  1:1556161/93‑1 (MQ=255)
                                                             gTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGt                            >  2:2592455/1‑93 (MQ=255)
                                                             gTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGt                            >  2:4311582/1‑93 (MQ=255)
                                                              ttCGAACGTTTCCTTAACCCGTAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTa                           >  2:1465985/1‑93 (MQ=255)
                                                              ttCGAACGTTTCCTTAACCCGGAACGTGTCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTa                           <  1:3958480/93‑1 (MQ=255)
                                                              ttCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTa                           <  2:1033046/93‑1 (MQ=255)
                                                               tCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGTCTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAg                          <  1:3725152/93‑1 (MQ=255)
                                                                cGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGc                         <  2:1249072/93‑1 (MQ=255)
                                                                cGAACGTGTCCTTAACCCGGAACTTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGc                         <  1:2739848/93‑1 (MQ=255)
                                                                  aaCGTTTCCTTACCCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCgg                       <  1:1258178/93‑1 (MQ=255)
                                                                   aCGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGa                      >  1:3696038/1‑93 (MQ=255)
                                                                   aCGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGa                      <  2:3169659/93‑1 (MQ=255)
                                                                   aCGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGa                      >  1:782004/1‑93 (MQ=255)
                                                                   aCGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGa                      >  1:539723/1‑93 (MQ=255)
                                                                    cGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGAc                     <  2:4558475/93‑1 (MQ=255)
                                                                     gTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACa                    >  1:2615801/1‑93 (MQ=255)
                                                                      tttCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACAt                   >  1:3480170/1‑93 (MQ=255)
                                                                      tttCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACAt                   <  1:1468209/93‑1 (MQ=255)
                                                                      tttCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACAt                   >  2:264374/1‑93 (MQ=255)
                                                                       ttCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATg                  <  2:1916017/93‑1 (MQ=255)
                                                                         ccTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTa                <  1:857314/93‑1 (MQ=255)
                                                                          cTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTAc               >  2:1566655/1‑93 (MQ=255)
                                                                           ttAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACg              <  1:3567765/93‑1 (MQ=255)
                                                                             aaCCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGt            <  1:3395397/93‑1 (MQ=255)
                                                                             aaCCCGGAACGTCCCTCCATGCCTGACTCCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGt            <  1:1440459/93‑1 (MQ=255)
                                                                                    gaCGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATg     <  1:3099364/92‑1 (MQ=255)
                                                                                    aaCGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTATGGTCGTGATg     <  1:848001/93‑1 (MQ=255)
                                                                                     aCGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGc    <  1:456781/93‑1 (MQ=255)
                                                                                       gtgcCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCgg  >  2:4034245/1‑93 (MQ=255)
                                                                                       gtgcCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCgg  <  2:2048029/93‑1 (MQ=255)
                                                                                       gtgcCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCgg  <  1:3991286/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGTCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCGG  >  NC_000913/206225‑206404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑