Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 206,483 A→G Y453C (TAC→TGC)  dnaE → DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913206,4830AG99.3% 452.5 / ‑6.2 147Y453C (TAC→TGC) dnaEDNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (75/71);  total (75/72)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

GTCGTGATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTACGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCG  >  NC_000913/206393‑206571
                                                                                          |                                                                                        
   gTGATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCt                                                                                     <  2:1668397/93‑1 (MQ=255)
    tGATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCtt                                                                                    >  2:2430634/1‑93 (MQ=255)
    tGATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCtt                                                                                    >  1:1208842/1‑93 (MQ=255)
     gATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCttt                                                                                   <  1:4027024/93‑1 (MQ=255)
     gATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCttt                                                                                   <  2:268091/93‑1 (MQ=255)
     gATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCttt                                                                                   <  2:4348390/93‑1 (MQ=255)
     gATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCttt                                                                                   <  1:2342400/93‑1 (MQ=255)
     gATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCttt                                                                                   >  2:712035/1‑93 (MQ=255)
     gATGAGGTATCGCAGATCATCCCCGTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCttt                                                                                   <  2:1671436/93‑1 (MQ=255)
      aTGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTg                                                                                  <  1:2458139/93‑1 (MQ=255)
        gCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTc                                                                                <  2:39501/93‑1 (MQ=255)
          ggTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGa                                                                              <  1:4504964/93‑1 (MQ=255)
           gTATCGCAGATCATCTCCTGCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGAt                                                                             <  2:216997/93‑1 (MQ=255)
           gTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGAt                                                                             <  1:553378/93‑1 (MQ=255)
           gTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGAt                                                                             >  1:3164172/1‑93 (MQ=255)
           gTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGAt                                                                             >  1:4148356/1‑93 (MQ=255)
             aTCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCg                                                                           <  2:3291825/93‑1 (MQ=255)
              tCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGt                                                                          >  2:3814729/1‑93 (MQ=255)
              tCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGt                                                                          >  1:1648085/1‑93 (MQ=255)
               cGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTa                                                                         <  2:4612764/93‑1 (MQ=255)
               cGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTa                                                                         <  2:3906945/93‑1 (MQ=255)
               cGCACATCATCACCTTCGCTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCCCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTa                                                                         <  2:236071/93‑1 (MQ=255)
                gCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTAt                                                                        <  2:2166283/93‑1 (MQ=255)
                  aGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCCTCCGTGGGGCTTTGTCGATCGTAtct                                                                      >  2:3780800/1‑93 (MQ=255)
                   gATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTAtctc                                                                     <  1:3484190/93‑1 (MQ=255)
                   gATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTAtctc                                                                     >  2:2789657/1‑93 (MQ=255)
                   gATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTAtctc                                                                     >  2:3692378/1‑93 (MQ=255)
                   gATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTAtctc                                                                     >  1:3186812/1‑93 (MQ=255)
                       atcaCCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGaaa                                                                 <  2:740850/93‑1 (MQ=255)
                        tcaCCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAAc                                                                >  1:201239/1‑93 (MQ=255)
                         caCCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACt                                                               <  1:2469871/93‑1 (MQ=255)
                         caCCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACt                                                               >  1:1622114/1‑93 (MQ=255)
                          aCCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTg                                                              >  2:2005779/1‑93 (MQ=255)
                           ccTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGa                                                             <  1:3728753/93‑1 (MQ=255)
                           ccTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGa                                                             <  1:3694538/93‑1 (MQ=255)
                            cTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGAt                                                            >  1:4135494/1‑93 (MQ=255)
                             ttCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATc                                                           >  1:2124925/1‑93 (MQ=255)
                             ttCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATc                                                           <  2:2829772/93‑1 (MQ=255)
                              tCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATcc                                                          <  2:2334015/93‑1 (MQ=255)
                              tCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGCAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATcc                                                          <  1:1762303/93‑1 (MQ=255)
                               cGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATccc                                                         <  1:3938729/93‑1 (MQ=255)
                               cGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATccc                                                         <  2:539723/93‑1 (MQ=255)
                                ggTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCCTCCGTGCGGCTTTGTCGGTCGTATCTCGACACTGATgccg                                                        >  2:111262/1‑93 (MQ=255)
                                 gTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATcccgc                                                       >  1:4492582/1‑93 (MQ=255)
                                  tACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATcccgcc                                                      >  2:3438675/1‑93 (MQ=255)
                                  tACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATcccgcc                                                      >  1:1960092/1‑93 (MQ=255)
                                  tACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATcccgcc                                                      >  2:4434562/1‑93 (MQ=255)
                                  tACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATaccgcc                                                      >  2:3130620/1‑93 (MQ=255)
                                    cAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATcccgcccg                                                    >  1:3987490/1‑93 (MQ=255)
                                    cAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATaccgcccg                                                    >  1:2404722/1‑93 (MQ=255)
                                      aTGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGac                                                  >  1:750396/1‑92 (MQ=255)
                                        ggcggcGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATcc                                                >  1:1495373/1‑93 (MQ=255)
                                         gcggcgAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCg                                               >  2:4191347/1‑93 (MQ=255)
                                          cggcgAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCgg                                              <  2:3330659/93‑1 (MQ=255)
                                          cggcgAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCgg                                              <  2:1402554/93‑1 (MQ=255)
                                             cgAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGa                                           <  1:3403619/93‑1 (MQ=255)
                                             cgAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGa                                           <  1:891567/93‑1 (MQ=255)
                                             cgAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGa                                           <  2:1156405/93‑1 (MQ=255)
                                               aaaGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATg                                         <  2:1198345/93‑1 (MQ=255)
                                                aaGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGa                                        >  2:4196949/1‑93 (MQ=255)
                                                aaGAGGTTATCCGCAACGTAGCCCGCGTTCTATGTCATCCGTGCGGCTTTGTCTATCTTATCTCGAAACTGATCCCGCCCGATCCGGGGATGa                                        <  2:4519748/93‑1 (MQ=255)
                                                 aGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGag                                       >  2:120918/1‑92 (MQ=255)
                                                   cGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGc                                     <  2:2606694/93‑1 (MQ=255)
                                                     gTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTg                                   >  2:46689/1‑93 (MQ=255)
                                                      tGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTgg                                  >  1:380123/1‑93 (MQ=255)
                                                        aTCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCg                                >  2:3669251/1‑93 (MQ=255)
                                                           cgcgACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGaaa                             <  2:3187798/93‑1 (MQ=255)
                                                            gcgACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAg                            <  1:2720241/93‑1 (MQ=255)
                                                              gACGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCg                          >  1:2738597/1‑93 (MQ=255)
                                                               gcGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGt                         <  1:1465985/92‑1 (MQ=255)
                                                               aCGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGt                         >  1:2534823/1‑93 (MQ=255)
                                                                cGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGtt                        <  2:1713449/93‑1 (MQ=255)
                                                                cGTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTCGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGtt                        <  2:4452025/93‑1 (MQ=255)
                                                                 gTAGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGttt                       >  1:1245052/1‑93 (MQ=255)
                                                                  tAGGCCGCGTGCTGGGGCATCCATGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTg                      >  1:1654562/1‑93 (MQ=255)
                                                                   aGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGa                     <  1:3295122/93‑1 (MQ=255)
                                                                   aGGCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGa                     >  2:1826761/1‑93 (MQ=255)
                                                                    ggCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGaa                    <  2:154334/93‑1 (MQ=255)
                                                                    ggCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGaa                    >  2:1714639/1‑93 (MQ=255)
                                                                    ggCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGaa                    <  2:46762/93‑1 (MQ=255)
                                                                    ggCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGaa                    >  1:3848821/1‑93 (MQ=255)
                                                                    ggCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGaa                    <  1:2539569/93‑1 (MQ=255)
                                                                     gCCGCGTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAag                   <  2:2507861/93‑1 (MQ=255)
                                                                       cgcgTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAagcc                 <  2:3452243/93‑1 (MQ=255)
                                                                       cgcgTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAagcc                 <  2:2360688/93‑1 (MQ=255)
                                                                       cgcgTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAagcc                 >  2:2812031/1‑93 (MQ=255)
                                                                        gcgTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATAACGCTGGCGAAAGCGTTTGAagccg                >  2:4613666/1‑93 (MQ=255)
                                                                        gcgTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGAGGATGACGCTGGCGAAAGCGTTTGAagccg                <  1:521666/93‑1 (MQ=255)
                                                                         ggTGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAagccga               <  2:1953292/92‑1 (MQ=255)
                                                                           tGCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGACCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAagccgagc             <  1:1805528/93‑1 (MQ=255)
                                                                            gCTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAagccgagcc            <  1:3441132/93‑1 (MQ=255)
                                                                             cTGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAagccgagccg           >  2:1860938/1‑93 (MQ=255)
                                                                              tGGGGCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGc          >  1:3034252/1‑93 (MQ=255)
                                                                                gggCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAg        <  1:1566655/93‑1 (MQ=255)
                                                                                gggCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAg        >  1:3505632/1‑93 (MQ=255)
                                                                                 ggCATCCGTGCGGCTTTGTCGATCGTATCTCGAACCTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGc       <  2:2856461/93‑1 (MQ=255)
                                                                                 ggCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGc       <  2:3286134/93‑1 (MQ=255)
                                                                                  gCATCCGTGCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCt      >  1:855579/1‑93 (MQ=255)
                                                                                    aTCCGTGCGGCTTGGTCGATCGTATCTCGAAACTGACCCCGCCCGATCCGGGGATGACCCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGc    <  2:452266/93‑1 (MQ=255)
                                                                                      ccGTACGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCg  <  1:3037680/93‑1 (MQ=255)
                                                                                          |                                                                                        
GTCGTGATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGATCCGCGACGTAGGCCGCGTGCTGGGGCATCCGTACGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCG  >  NC_000913/206393‑206571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑