Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,089,888	T→C	L257P (CTC→CCC)	hisD →	L‑histidinal:NAD+ oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,089,888	0	T	C	100.0%	301.5 / NA	95	L257P (CTC→CCC)	hisD	L‑histidinal:NAD+ oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/53);  total (42/53)

TGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAC  >  NC_000913/2089799‑2089977                                                                                          |                                                                                          tGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGccctc                                                                                        <  1:1584504/93‑1 (MQ=255) tGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGccctc                                                                                        >  1:667692/1‑93 (MQ=255)    ggcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCa                                                                                     >  1:360317/1‑93 (MQ=255)     gcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTCCGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAg                                                                                    <  2:4280649/93‑1 (MQ=255)     gcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAg                                                                                    <  2:358636/93‑1 (MQ=255)     gcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAg                                                                                    <  1:3469014/93‑1 (MQ=255)     gcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAg                                                                                    <  1:2397546/93‑1 (MQ=255)     gcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAg                                                                                    <  1:1790587/93‑1 (MQ=255)      cgatcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAgg                                                                                   <  1:4522630/93‑1 (MQ=255)       gatcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGc                                                                                  <  1:2373552/93‑1 (MQ=255)       gatcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGc                                                                                  <  1:3729242/93‑1 (MQ=255)       gatcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGc                                                                                  <  1:1727759/93‑1 (MQ=255)        atcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCt                                                                                 <  1:705246/93‑1 (MQ=255)        atcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCt                                                                                 <  1:2762416/93‑1 (MQ=255)        atcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCt                                                                                 >  1:3573596/1‑93 (MQ=255)         tcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTg                                                                                <  1:4292698/93‑1 (MQ=255)           gatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGaa                                                                              <  2:282439/93‑1 (MQ=255)            atatGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAac                                                                             >  2:1396620/1‑93 (MQ=255)             tatGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAaca                                                                            >  1:3432167/1‑93 (MQ=255)             tatGCCCGCAGACCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGCATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAaca                                                                            <  2:2697219/93‑1 (MQ=255)                gCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACgg                                                                         >  1:586840/1‑93 (MQ=255)                gCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACgg                                                                         <  2:1835395/93‑1 (MQ=255)                gCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACgg                                                                         >  1:4301117/1‑93 (MQ=255)                 cccGCAGGCCCGTCGGAAGTGATGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGc                                                                        >  2:2983536/1‑93 (MQ=255)                    gCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCg                                                                     >  1:3695378/1‑93 (MQ=255)                      aGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGa                                                                   <  1:2162542/93‑1 (MQ=255)                       gtcccGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTCCGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGAc                                                                  <  1:3472063/91‑1 (MQ=255)                       ggCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGAc                                                                  >  1:685251/1‑93 (MQ=255)                       ggCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGAc                                                                  >  1:1576423/1‑93 (MQ=255)                       ggCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGAc                                                                  <  1:2132674/93‑1 (MQ=255)                          cggTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTca                                                               <  1:965599/91‑1 (MQ=255)                          ccGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTca                                                               <  2:1302235/93‑1 (MQ=255)                           cGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTcac                                                              <  1:4454412/93‑1 (MQ=255)                           cGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTcac                                                              <  2:2800400/93‑1 (MQ=255)                             tCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAg                                                            <  2:2492814/93‑1 (MQ=255)                               ggAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAggg                                                          >  2:1227098/1‑92 (MQ=255)                               ggAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAggg                                                          >  2:2566732/1‑92 (MQ=255)                               ggAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGt                                                          <  2:1823098/93‑1 (MQ=255)                               ggAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGt                                                          <  1:4117922/93‑1 (MQ=255)                               ggAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGt                                                          <  2:1475330/93‑1 (MQ=255)                                gAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTg                                                         >  1:1314927/1‑93 (MQ=255)                                 aaGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGa                                                        <  1:1698628/93‑1 (MQ=255)                                  aGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAt                                                       <  2:3739481/93‑1 (MQ=255)                                   gTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAtt                                                      >  1:4043772/1‑93 (MQ=255)                                    tGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAttt                                                     <  1:2646500/93‑1 (MQ=255)                                    tGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAttt                                                     <  1:2816110/93‑1 (MQ=255)                                       tGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTAc                                                  >  2:3077883/1‑93 (MQ=255)                                       tGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTAc                                                  >  1:2688139/1‑93 (MQ=255)                                        ggTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACt                                                 <  2:1987621/93‑1 (MQ=255)                                          tGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGa                                               <  1:1100832/93‑1 (MQ=255)                                          tGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGa                                               >  2:2215246/1‑93 (MQ=255)                                          tGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGa                                               >  1:3551959/1‑93 (MQ=255)                                           gATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGAc                                              >  2:2704880/1‑93 (MQ=255)                                           gATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGAc                                              <  1:1852383/93‑1 (MQ=255)                                            aTTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACg                                             >  1:581987/1‑93 (MQ=255)                                             ttGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGGTTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGGGATTTTACTGACGc                                            >  2:1913392/1‑93 (MQ=255)                                             ttGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGc                                            <  1:2152994/93‑1 (MQ=255)                                              tGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGcc                                           <  2:141000/93‑1 (MQ=255)                                              tGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGcc                                           <  2:3250587/93‑1 (MQ=255)                                               gCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGccc                                          <  1:1362072/93‑1 (MQ=255)                                                cTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCg                                         <  1:3174187/93‑1 (MQ=255)                                                 tGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTTCCCTCTCAGGCTGAACACGGCCCGGACTCACCGGGGATTTTACTAACGCCCgc                                        >  2:2741548/1‑93 (MQ=255)                                                  gACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgct                                       >  2:2812923/1‑93 (MQ=255)                                                   aCAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGTCCCGGACTCACAGGTGATTTTACTGACGCCCgctg                                      >  1:1338049/1‑93 (MQ=255)                                                    cAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgctgc                                     >  1:3744332/1‑93 (MQ=255)                                                      gcggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGctgctg                                   >  1:443030/1‑93 (MQ=255)                                                      gcggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGctgctg                                   >  1:1371859/1‑93 (MQ=255)                                                        ggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGat                                 >  2:2403897/1‑93 (MQ=255)                                                          cgcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGatat                               <  1:162633/93‑1 (MQ=255)                                                           gcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATg                              <  2:1127321/93‑1 (MQ=255)                                                           gcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATg                              >  1:3394080/1‑93 (MQ=255)                                                            cTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATgg                             <  1:4654641/93‑1 (MQ=255)                                                             tACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGgc                            <  1:847485/93‑1 (MQ=255)                                                              aCGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGgcg                           >  1:2026851/1‑93 (MQ=255)                                                                 ccGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgt                        >  1:4014691/1‑93 (MQ=255)                                                                 ccGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgt                        >  2:4549902/1‑93 (MQ=255)                                                                   ggATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcg                      >  1:395407/1‑93 (MQ=255)                                                                     aTTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgcg                    <  1:4519503/93‑1 (MQ=255)                                                                      tttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgcgt                   <  2:792204/93‑1 (MQ=255)                                                                      tttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGagcgtcgcgt                   >  1:212784/1‑93 (MQ=255)                                                                       ttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGtt                  <  2:426058/93‑1 (MQ=255)                                                                         cGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGc                <  2:399343/93‑1 (MQ=255)                                                                          gTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGcc               >  1:3457919/1‑93 (MQ=255)                                                                           tGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCg              >  1:1245806/1‑93 (MQ=255)                                                                             gCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAg            >  2:1972320/1‑93 (MQ=255)                                                                             gCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAg            <  2:895040/93‑1 (MQ=255)                                                                             gCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGCTGCTTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAg            >  1:2836214/1‑93 (MQ=255)                                                                               ttCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGc          <  2:942265/93‑1 (MQ=255)                                                                               ttCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGc          >  2:1551522/1‑93 (MQ=255)                                                                                 cTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCg        <  2:3121053/93‑1 (MQ=255)                                                                                  tGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGACGt       >  2:1681642/1‑93 (MQ=255)                                                                                     tttGCCCTCTCAGGCTGAACACGGCCCGGCCTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGGGTCGCGTTGCCGAGCCCGTCGa    <  2:2257035/93‑1 (MQ=255)                                                                                     tttGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGa    >  2:1963744/1‑93 (MQ=255)                                                                                     tttGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGa    >  1:3803504/1‑93 (MQ=255)                                                                                      ttGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGaa   <  1:452159/93‑1 (MQ=255)                                                                                      ttGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGaa   <  1:3062570/93‑1 (MQ=255)                                                                                       tGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAc  <  2:1190886/93‑1 (MQ=255)                                                                                          |                                                                                          TGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAC  >  NC_000913/2089799‑2089977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑