BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,094,539	T→C	V225A (GTT→GCT)	hisF →	imidazole glycerol phosphate synthase holoenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,094,539	0	T	C	100.0%	416.2 / NA	132	V225A (GTT→GCT)	hisF	imidazole glycerol phosphate synthase holoenzyme
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (52/80); total (52/80)

Only 100 of 136 total aligned reads displayed.

AGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGA  >  NC_000913/2094450‑2094628

aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTgc                                                                                        >  1:990327/1‑92 (MQ=255)

aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGa                                                                                        <  2:491246/93‑1 (MQ=255)

   gCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACgg                                                                                     <  1:2502376/93‑1 (MQ=255)

   gCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACgg                                                                                     <  1:3643434/93‑1 (MQ=255)

   gCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACgg                                                                                     >  1:2099943/1‑93 (MQ=255)

   gCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCATTCCGCGATGCCGACGCTGACgg                                                                                     >  2:1009710/1‑93 (MQ=255)

      tGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcgc                                                                                  <  2:1840337/93‑1 (MQ=255)

      tGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcgc                                                                                  >  1:698210/1‑93 (MQ=255)

       gAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcgcg                                                                                 >  1:4372126/1‑93 (MQ=255)

       gAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcgcg                                                                                 >  1:4222306/1‑93 (MQ=255)

         aGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCt                                                                               <  1:1495936/93‑1 (MQ=255)

         aGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCt                                                                               <  1:579004/93‑1 (MQ=255)

           tttGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTgg                                                                             >  1:2290226/1‑93 (MQ=255)

               ccACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGc                                                                         >  1:1608527/1‑93 (MQ=255)

                cACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCt                                                                        >  1:498608/1‑93 (MQ=255)

                  cGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTc                                                                      <  2:4466799/93‑1 (MQ=255)

                   gTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTcc                                                                     >  1:647554/1‑93 (MQ=255)

                   gTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTcc                                                                     <  2:2323082/93‑1 (MQ=255)

                   gTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTcc                                                                     <  1:4143640/93‑1 (MQ=255)

                   gTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTcc                                                                     <  1:1461630/93‑1 (MQ=255)

                    tCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCg                                                                    >  1:1397649/1‑93 (MQ=255)

                     cccGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGt                                                                   <  2:497802/93‑1 (MQ=255)

                      gcGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTa                                                                  >  2:4503414/2‑93 (MQ=255)

                      ccGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTa                                                                  <  1:758425/93‑1 (MQ=255)

                      ccGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTa                                                                  >  2:4581340/1‑93 (MQ=255)

                      ccGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTa                                                                  >  2:3146809/1‑93 (MQ=255)

                      ccGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTa                                                                  <  1:2149190/93‑1 (MQ=255)

                       cGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 >  2:3692347/1‑93 (MQ=255)

                       cGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 <  2:3509433/93‑1 (MQ=255)

                       cGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 <  2:2970023/93‑1 (MQ=255)

                       cGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 <  2:2782169/93‑1 (MQ=255)

                       cGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 <  2:1797784/93‑1 (MQ=255)

                       cGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 >  1:121808/1‑93 (MQ=255)

                         cTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTc                                                               >  1:3659493/1‑93 (MQ=255)

                         cTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTc                                                               >  2:4450822/1‑93 (MQ=255)

                           gATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCca                                                             <  2:2507741/93‑1 (MQ=255)

                           gATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCca                                                             <  1:4503090/93‑1 (MQ=255)

                            aTTGCCTCCGGTGGCGCGGGCACCATGGATCACTTCCTCGAAGCCTTCCGCGATGCCGCCGCTGACGGCGCGCTGGCAGCTTCCGTATTCcac                                                            <  2:2583269/93‑1 (MQ=255)

                            aTTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCcac                                                            <  2:2767429/93‑1 (MQ=255)

                            aTTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCcac                                                            >  1:4387809/1‑93 (MQ=255)

                             ttGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCcaca                                                           >  2:1028275/1‑93 (MQ=255)

                              tGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaa                                                          >  2:4569288/1‑93 (MQ=255)

                                 cTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaaca                                                       <  1:2096272/93‑1 (MQ=255)

                                   ccGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCAcaaacaaa                                                     <  2:2655424/93‑1 (MQ=255)

                                   ccGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCAcaaacaaa                                                     <  1:263445/93‑1 (MQ=255)

                                   ccGGTAGCCCGGGCACCATGGAACCCTTCCTCGAAGCCGTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCAcaaacaaa                                                     <  2:475808/93‑1 (MQ=255)

                                    cGGTGGCGCGGGCACCATGCAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    <  2:4147054/93‑1 (MQ=255)

                                     ggTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaata                                                   <  1:2916894/93‑1 (MQ=255)

                                     ggTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaata                                                   <  2:799312/93‑1 (MQ=255)

                                     ggTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaata                                                   <  2:1618537/93‑1 (MQ=255)

                                        ggCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATc                                                >  2:3942988/1‑93 (MQ=255)

                                           gcgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAat                                             <  1:4297466/93‑1 (MQ=255)

                                           gcgGCCACCATGCAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAat                                             <  2:251896/93‑1 (MQ=255)

                                            cgGGCTCCATGGAACACTTCTTCGAAGCCTACCGCTATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAata                                            <  2:1356236/93‑1 (MQ=255)

                                            cgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAata                                            <  1:2473237/93‑1 (MQ=255)

                                            cgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAata                                            >  2:3356452/1‑93 (MQ=255)

                                              ggCACCATGGAACACTTCCTCAAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAAACAATAtt                                          <  2:1375580/93‑1 (MQ=255)

                                                gaCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTgg                                        <  1:3861630/92‑1 (MQ=255)

                                                cACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTgg                                        <  2:1749750/93‑1 (MQ=255)

                                                cACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTgg                                        <  1:1909442/93‑1 (MQ=255)

                                                cACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTgg                                        <  1:4194566/93‑1 (MQ=255)

                                                 cccATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGt                                       <  2:665626/92‑1 (MQ=255)

                                                 cccATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGt                                       <  1:3186438/92‑1 (MQ=255)

                                                  ccATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTg                                      >  2:3122902/1‑93 (MQ=255)

                                                   cATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGa                                     >  2:3569568/1‑93 (MQ=255)

                                                   cATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGa                                     >  1:4007958/1‑93 (MQ=255)

                                                    aTGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGaa                                    <  2:1585210/93‑1 (MQ=255)

                                                     tGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAt                                   >  2:368594/1‑93 (MQ=255)

                                                     tGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAt                                   <  2:2471674/93‑1 (MQ=255)

                                                      ggAACACTTCCTCGAAGCCTGCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAtt                                  <  1:609282/93‑1 (MQ=255)

                                                       gAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTa                                 <  2:3678417/93‑1 (MQ=255)

                                                        aaCACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaa                                >  2:4324865/1‑93 (MQ=255)

                                                          cccTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaaa                              <  2:292785/91‑1 (MQ=255)

                                                          cacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaaa                              <  1:621190/93‑1 (MQ=255)

                                                          cacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaaa                              >  2:3980421/1‑93 (MQ=255)

                                                           acTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaaaa                             >  2:1821672/1‑92 (MQ=255)

                                                           acTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAg                             <  2:2856683/93‑1 (MQ=255)

                                                           acTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAg                             <  1:454181/93‑1 (MQ=255)

                                                           acTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAg                             <  1:3284086/93‑1 (MQ=255)

                                                            cTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGc                            >  2:1138740/1‑93 (MQ=255)

                                                            cTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGc                            <  1:349706/93‑1 (MQ=255)

                                                            cTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGc                            <  1:301718/93‑1 (MQ=255)

                                                                cTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTAc                        >  1:2468849/1‑93 (MQ=255)

                                                                cTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTAc                        >  1:2649357/1‑93 (MQ=255)

                                                                 tCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTAcc                       >  2:874207/1‑93 (MQ=255)

                                                                  cGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCt                      <  2:2562060/93‑1 (MQ=255)

                                                                   gAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTg                     >  2:688857/1‑93 (MQ=255)

                                                                     aGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGc                   <  2:4330756/93‑1 (MQ=255)

                                                                      gCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCa                  >  2:4294429/1‑93 (MQ=255)

                                                                         ttCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAaca               >  1:3605656/1‑93 (MQ=255)

                                                                         ttCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAaca               >  1:2215825/1‑93 (MQ=255)

                                                                          tCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAacac              >  2:2190177/1‑93 (MQ=255)

                                                                           ccGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAAcaca             <  2:1431045/93‑1 (MQ=255)

                                                                             gcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAgg           <  1:1164084/93‑1 (MQ=255)

                                                                             gcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAgg           <  1:2006519/93‑1 (MQ=255)

                                                                               gATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGc         >  1:3925564/1‑93 (MQ=255)

                                                                                  gCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTg      <  1:2028522/93‑1 (MQ=255)

                                                                                   ccGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTgg     <  2:850011/93‑1 (MQ=255)

                                                                                      aCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgaga  <  1:2628796/93‑1 (MQ=255)

                                                                                      aCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgaga  >  2:279259/1‑93 (MQ=255)

AGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGA  >  NC_000913/2094450‑2094628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`