Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,108,194 T→C Q283R (CAG→CGG)  rfbA ← glucose‑1‑phosphate thymidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,108,1940TC99.3% 424.6 / ‑4.9 135Q283R (CAG→CGG) rfbAglucose‑1‑phosphate thymidylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (64/70);  total (65/70)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 136 total aligned reads displayed.

AATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGA  >  NC_000913/2108105‑2108283
                                                                                         |                                                                                         
  tgctctTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCa                                                                                      <  2:2984625/91‑1 (MQ=255)
  tACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCa                                                                                      >  2:3237789/1‑93 (MQ=255)
    ctctTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATa                                                                                    <  2:4659840/93‑1 (MQ=255)
    ctctTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATa                                                                                    >  1:3503927/1‑93 (MQ=255)
    ctctTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATa                                                                                    >  2:1533595/1‑93 (MQ=255)
    ctctTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATa                                                                                    >  2:2174805/1‑93 (MQ=255)
     tctTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATaa                                                                                   <  1:4099744/93‑1 (MQ=255)
     tctTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATaa                                                                                   <  2:236025/93‑1 (MQ=255)
           cTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTc                                                                             >  1:1004393/1‑93 (MQ=255)
           cTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTc                                                                             <  1:2751847/93‑1 (MQ=255)
            tCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATTCCGCCCATAATTATTCt                                                                            <  2:3091409/93‑1 (MQ=255)
             ccAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCtt                                                                           <  1:1330669/93‑1 (MQ=255)
               aGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTtta                                                                         <  1:1001183/93‑1 (MQ=255)
                gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        <  2:3358348/93‑1 (MQ=255)
                gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        >  2:3660206/1‑93 (MQ=255)
                gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        >  2:4004993/1‑93 (MQ=255)
                gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        >  1:2479193/1‑93 (MQ=255)
                 aaTTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatt                                                                       >  2:783097/1‑93 (MQ=255)
                  aTTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatta                                                                      <  2:2456097/93‑1 (MQ=255)
                   ttAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAg                                                                     >  1:1744872/1‑93 (MQ=255)
                   atAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAg                                                                     <  2:3586014/92‑1 (MQ=255)
                    tAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGt                                                                    <  1:356999/93‑1 (MQ=255)
                      gCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTgg                                                                  >  2:1965323/1‑93 (MQ=255)
                       cacaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGt                                                                 >  1:3549142/1‑93 (MQ=255)
                        acaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTa                                                                <  2:529003/93‑1 (MQ=255)
                        acaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTa                                                                >  1:849337/1‑93 (MQ=255)
                        acaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTa                                                                >  1:816138/1‑93 (MQ=255)
                         caTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTAc                                                               <  2:2438452/93‑1 (MQ=255)
                            cTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGc                                                            <  2:1815610/93‑1 (MQ=255)
                             ttCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCt                                                           >  1:1675788/1‑93 (MQ=255)
                               cAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATCCCGCCCATAATTATTCTTTATTAGTGGTACAGCTaa                                                         <  2:4499631/93‑1 (MQ=255)
                               cAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTaa                                                         >  2:209410/1‑93 (MQ=255)
                               cAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTaa                                                         <  1:3874776/93‑1 (MQ=255)
                                 aTTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAAtt                                                       <  1:1911835/93‑1 (MQ=255)
                                 aTTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAAtt                                                       <  1:3136984/93‑1 (MQ=255)
                                  tttCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAAttt                                                      <  2:1105144/93‑1 (MQ=255)
                                    tCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTc                                                    <  1:2136378/93‑1 (MQ=255)
                                     cAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCt                                                   >  2:2628910/1‑93 (MQ=255)
                                       gTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTa                                                 >  1:1331463/1‑93 (MQ=255)
                                        ttCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTAc                                                <  1:1294071/93‑1 (MQ=255)
                                        ttCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTAc                                                <  2:2341832/93‑1 (MQ=255)
                                         tCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTAATAGTGGTACAGCTAATTTTCTTACt                                               >  1:4528488/1‑93 (MQ=255)
                                          cTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACtt                                              <  2:362326/93‑1 (MQ=255)
                                          cTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACtt                                              <  1:2909306/93‑1 (MQ=255)
                                           tAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTg                                             >  2:2776411/1‑93 (MQ=255)
                                           tAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTg                                             >  2:3810596/1‑93 (MQ=255)
                                            aaTCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGc                                            <  1:369476/93‑1 (MQ=255)
                                             aTCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCt                                           >  2:2363724/1‑93 (MQ=255)
                                             aTCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCt                                           <  2:349885/93‑1 (MQ=255)
                                              tCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTc                                          >  2:164944/1‑93 (MQ=255)
                                                 cattcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAAc                                       >  2:1493305/1‑93 (MQ=255)
                                                  attcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACa                                      >  2:473876/1‑93 (MQ=255)
                                                   ttcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACAt                                     >  1:4680836/1‑93 (MQ=255)
                                                   ttcattAATTTGAATCCTTAGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACAt                                     >  1:1519242/1‑93 (MQ=255)
                                                    tcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATc                                    >  2:4371065/1‑93 (MQ=255)
                                                      attAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTATTTGTCTTACTTGCTCAACATCaa                                  <  2:3829563/93‑1 (MQ=255)
                                                      attAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCaa                                  <  2:2051441/93‑1 (MQ=255)
                                                      attAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCaa                                  >  1:2555930/1‑93 (MQ=255)
                                                      attAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCaa                                  >  2:4182438/1‑93 (MQ=255)
                                                       ttAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCTACATCAAt                                 >  1:3276472/1‑93 (MQ=255)
                                                       ttAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAAt                                 >  2:4293813/1‑93 (MQ=255)
                                                         aaTTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATaa                               <  2:3123145/93‑1 (MQ=255)
                                                          aTTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATaaa                              >  2:2988594/1‑93 (MQ=255)
                                                          aTTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATaaa                              <  2:2740104/93‑1 (MQ=255)
                                                           tttGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATaaaa                             >  2:1232389/1‑93 (MQ=255)
                                                               aaTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCtt                         <  1:1220506/93‑1 (MQ=255)
                                                                aTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCttt                        >  1:2660199/1‑93 (MQ=255)
                                                                 tCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAACCCTTTa                       <  2:2616460/93‑1 (MQ=255)
                                                                  ccTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTAc                      >  1:1095525/1‑93 (MQ=255)
                                                                  ccTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTAc                      <  1:2456512/93‑1 (MQ=255)
                                                                  ccTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTAc                      <  1:2796582/93‑1 (MQ=255)
                                                                     tCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaa                   >  2:445212/1‑93 (MQ=255)
                                                                     tCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaa                   >  1:19775/1‑93 (MQ=255)
                                                                      cGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaaa                  <  1:4659765/93‑1 (MQ=255)
                                                                       gTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAGAt                 >  1:793504/1‑93 (MQ=255)
                                                                       gTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAt                 <  2:769822/93‑1 (MQ=255)
                                                                       gTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAt                 <  1:2216164/93‑1 (MQ=255)
                                                                       gTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAt                 >  2:3344369/1‑93 (MQ=255)
                                                                       gTCATTTTATAAAGATACCGCCCATAATGATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTCCTCAACATCAATAAAACCTTTACGAAAt                 <  2:3978369/93‑1 (MQ=255)
                                                                         cATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGc               <  2:3602696/93‑1 (MQ=255)
                                                                          aTTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCa              >  1:4497754/1‑93 (MQ=255)
                                                                          aTTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCa              >  1:4148455/1‑93 (MQ=255)
                                                                           ttttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGACATGCaa             >  2:4504375/1‑93 (MQ=255)
                                                                           ttttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCaa             <  1:1764098/93‑1 (MQ=255)
                                                                            tttATAAAGATACCGCCCATAGTTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAt            <  2:2601001/93‑1 (MQ=255)
                                                                            tttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAt            >  1:3097051/1‑93 (MQ=255)
                                                                             ttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtc           <  1:4514674/93‑1 (MQ=255)
                                                                                taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  2:2082813/93‑1 (MQ=255)
                                                                                taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  2:1243402/93‑1 (MQ=255)
                                                                                taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  2:3020517/93‑1 (MQ=255)
                                                                                 aaaGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCtt       <  1:247865/93‑1 (MQ=255)
                                                                                  aaGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTc      >  1:2294756/1‑93 (MQ=255)
                                                                                   aGATACCGCCCATATTTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTca     >  2:2793459/1‑93 (MQ=255)
                                                                                   aGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTca     <  1:4037811/93‑1 (MQ=255)
                                                                                   aGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTca     >  1:127449/1‑93 (MQ=255)
                                                                                   aGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTca     <  2:1645447/93‑1 (MQ=255)
                                                                                    gATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcag    >  2:3220289/1‑93 (MQ=255)
                                                                                    gATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcag    <  2:2228825/93‑1 (MQ=255)
                                                                                      tACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcagga  <  1:3823768/93‑1 (MQ=255)
                                                                                      tACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcagga  <  2:2848048/93‑1 (MQ=255)
                                                                                         |                                                                                         
AATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGA  >  NC_000913/2108105‑2108283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑