BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,123,880	T→C	Q123R (CAG→CGG)	wcaH ←	GDP‑mannose mannosyl hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,123,880	0	T	C	100.0%	418.7 / NA	132	Q123R (CAG→CGG)	wcaH	GDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (65/67); total (65/67)

Only 100 of 141 total aligned reads displayed.

TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAA  >  NC_000913/2123790‑2123968

ttCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCAtgccgc                                                                                        <  1:4255076/93‑1 (MQ=255)

ttCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCAtgccgc                                                                                        >  2:4435077/1‑93 (MQ=255)

 tctcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATgccgct                                                                                       >  1:2933400/1‑93 (MQ=255)

 tctcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATgccgct                                                                                       >  1:2842439/1‑93 (MQ=255)

   tcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCa                                                                                     >  2:3012991/1‑93 (MQ=255)

    cAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCAt                                                                                    <  1:817444/93‑1 (MQ=255)

    cAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCAt                                                                                    >  2:3312757/1‑93 (MQ=255)

        gagaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCgg                                                                                >  1:31711/1‑93 (MQ=255)

             aaTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTa                                                                           >  2:4100602/1‑93 (MQ=255)

              aTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTaa                                                                          >  2:880133/1‑93 (MQ=255)

               tAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGTGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAc                                                                         >  2:4547182/1‑93 (MQ=255)

               tAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAc                                                                         >  2:1390559/1‑93 (MQ=255)

               tAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAc                                                                         >  2:2151242/1‑93 (MQ=255)

               tAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAc                                                                         <  1:162119/93‑1 (MQ=255)

               tAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAc                                                                         <  2:2797356/93‑1 (MQ=255)

                aGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACa                                                                        <  1:2726220/93‑1 (MQ=255)

                  gcgcGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGc                                                                      <  1:3544216/93‑1 (MQ=255)

                   cgcgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGct                                                                     >  1:2381942/1‑93 (MQ=255)

                    gcgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGctc                                                                    <  2:1165193/93‑1 (MQ=255)

                    gcgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGctc                                                                    <  2:3012646/93‑1 (MQ=255)

                     cgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGctct                                                                   >  1:1738212/1‑93 (MQ=255)

                       gCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttc                                                                 >  2:4572198/1‑93 (MQ=255)

                       gCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttc                                                                 >  1:4596859/1‑93 (MQ=255)

                       gCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttc                                                                 <  2:2566859/93‑1 (MQ=255)

                       gCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttc                                                                 >  1:3503014/1‑93 (MQ=255)

                       gCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttc                                                                 <  1:2798134/93‑1 (MQ=255)

                         tGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTcttctt                                                               >  1:2348558/1‑93 (MQ=255)

                         tGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTcttctt                                                               >  2:4198954/1‑93 (MQ=255)

                         tGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTcttctt                                                               >  1:4174226/1‑93 (MQ=255)

                          gTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCttcttc                                                              >  2:3847231/1‑93 (MQ=255)

                           ttAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTcc                                                             >  2:1603345/1‑93 (MQ=255)

                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                           >  1:3656215/1‑93 (MQ=255)

                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                           >  2:841841/1‑93 (MQ=255)

                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                           >  2:1247927/1‑93 (MQ=255)

                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                           <  1:2094289/93‑1 (MQ=255)

                              gCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGAt                                                          >  2:3672986/1‑93 (MQ=255)

                              gCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGCAACAGCTCTTCCTCCGAt                                                          >  2:369922/1‑93 (MQ=255)

                                aTGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAc                                                        <  2:3205869/93‑1 (MQ=255)

                                aTGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAc                                                        >  2:1048702/1‑93 (MQ=255)

                                aTGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAc                                                        <  2:140995/93‑1 (MQ=255)

                                aTGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAc                                                        <  1:553448/93‑1 (MQ=255)

                                  gAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATActc                                                      >  2:1579835/1‑93 (MQ=255)

                                     cATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGa                                                   <  2:4400498/93‑1 (MQ=255)

                                            aatGGAGAGCAGCGCGTCCGACGTCAGCCAGCGGCAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaca                                            >  2:2959683/3‑91 (MQ=255)

                                             cTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaaa                                           <  2:557052/93‑1 (MQ=255)

                                             cTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaaa                                           >  2:4140353/1‑93 (MQ=255)

                                             cTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaaa                                           >  2:2754517/1‑93 (MQ=255)

                                             cTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaaa                                           <  2:2223320/93‑1 (MQ=255)

                                              tGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAc                                          <  2:2862114/93‑1 (MQ=255)

                                              tGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAc                                          <  2:2544129/93‑1 (MQ=255)

                                               ggCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAcc                                         >  2:1426387/1‑93 (MQ=255)

                                                gCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCg                                        <  1:2747696/93‑1 (MQ=255)

                                                gCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCg                                        <  1:4524846/93‑1 (MQ=255)

                                                  gAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAg                                      <  2:4231611/93‑1 (MQ=255)

                                                  gAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAg                                      <  1:424721/93‑1 (MQ=255)

                                                  gAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAACGAg                                      >  1:3258270/1‑93 (MQ=255)

                                                   agcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGc                                     >  1:1033925/1‑93 (MQ=255)

                                                   agcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGc                                     <  2:347463/93‑1 (MQ=255)

                                                    gcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGca                                    >  1:1648821/1‑93 (MQ=255)

                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacca                                 >  2:2238982/1‑93 (MQ=255)

                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacca                                 >  1:3607721/1‑93 (MQ=255)

                                                        cgcgTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcaccac                                <  1:1494351/93‑1 (MQ=255)

                                                         gcgTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACcaca                               <  2:4181491/93‑1 (MQ=255)

                                                         gcgTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACcaca                               >  1:451873/1‑93 (MQ=255)

                                                         ccgTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACcaca                               <  2:2361541/92‑1 (MQ=255)

                                                           gTCCGACTTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATa                             <  1:200604/93‑1 (MQ=255)

                                                           gTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATa                             >  2:2253170/1‑93 (MQ=255)

                                                            tCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATag                            >  2:475834/1‑93 (MQ=255)

                                                             ccGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagt                           >  1:4037802/1‑93 (MQ=255)

                                                              cGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtg                          >  1:981566/1‑93 (MQ=255)

                                                               gACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtga                         <  2:210255/93‑1 (MQ=255)

                                                               gACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtga                         <  2:3738367/93‑1 (MQ=255)

                                                               gACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtga                         >  2:1807964/1‑93 (MQ=255)

                                                               gACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtga                         <  2:1178462/93‑1 (MQ=255)

                                                                aCGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtgag                        >  1:539604/1‑93 (MQ=255)

                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                     <  2:2946971/93‑1 (MQ=255)

                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                     <  2:1220808/93‑1 (MQ=255)

                                                                    cagccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtggt                    <  2:1091012/93‑1 (MQ=255)

                                                                    cagccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtggt                    <  1:2640939/93‑1 (MQ=255)

                                                                      gccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGa                  <  1:1426124/93‑1 (MQ=255)

                                                                       ccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGaa                 >  2:2277635/1‑93 (MQ=255)

                                                                        cagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGaaa                <  1:3568389/93‑1 (MQ=255)

                                                                          gcgcTAATCGTCATGCCGCTCATCCGGCAGTACCAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATc              <  2:3092503/93‑1 (MQ=255)

                                                                          gcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATc              <  1:4100602/93‑1 (MQ=255)

                                                                          gcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATc              >  2:2239594/1‑93 (MQ=255)

                                                                           cGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATcc             <  1:3848696/93‑1 (MQ=255)

                                                                           cGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATcc             <  1:3180214/93‑1 (MQ=255)

                                                                            ggTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCg            <  2:1775753/93‑1 (MQ=255)

                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAACCCGAGCACCACATAGTGAGTGGTGAAATCCGt           <  1:332614/93‑1 (MQ=255)

                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGt           >  2:683637/1‑93 (MQ=255)

                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGt           <  1:4435077/93‑1 (MQ=255)

                                                                                aTCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGcc        >  1:1019017/1‑93 (MQ=255)

                                                                                aTCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGcc        >  2:3831252/1‑93 (MQ=255)

                                                                                aTCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGcc        >  2:2928499/1‑93 (MQ=255)

                                                                                aTCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGcc        >  1:3827319/1‑93 (MQ=255)

                                                                                   gTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCaga     <  2:2297357/93‑1 (MQ=255)

                                                                                   gTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCaga     <  2:3580455/93‑1 (MQ=255)

                                                                                    tCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCagag    >  1:708526/1‑93 (MQ=255)

                                                                                      aTGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGaa  >  2:2589814/1‑93 (MQ=255)

                                                                                      aTGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGaa  >  1:1183145/1‑93 (MQ=255)

TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAA  >  NC_000913/2123790‑2123968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`