BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,208,331	A→G	S404S (TCA→TCG)	b2122 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,208,331	0	A	G	99.1%	351.4 / NA	116	S404S (TCA→TCG)	b2122	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (35/80); minor base C (1/0); total (36/80)
Fisher's exact test for biased strand distribution p-value = 3.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

GCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑ACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208241‑2208420

gCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTc‑gca                                                                                         <  2:3784379/93‑1 (MQ=255)

 ccGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCaa                                                                                        <  1:629900/93‑1 (MQ=255)

   gcgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAagc                                                                                      <  2:4292371/93‑1 (MQ=255)

   gcgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTCC‑GCAagc                                                                                      <  2:4455420/93‑1 (MQ=255)

    cgAAGTGAACAGGAAAACTCACCGGCAGCTACTCGTCGTCTGTTTAGGTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAagca                                                                                     <  1:2935553/93‑1 (MQ=255)

    cgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGGAAATTGTTTT‑GCAagca                                                                                     >  2:751080/1‑93 (MQ=255)

    cgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAagca                                                                                     <  1:2022302/93‑1 (MQ=255)

    cgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAagca                                                                                     <  1:3267067/93‑1 (MQ=255)

     gAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAagcag                                                                                    <  1:2368330/93‑1 (MQ=255)

      aaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGAAAATTTTTTC‑CCaacagc                                                                                    >  2:3193569/1‑89 (MQ=255)

      aaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAagcagc                                                                                   <  2:917683/93‑1 (MQ=255)

       aGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTT‑GCAAgcagca                                                                                  >  2:1373715/1‑93 (MQ=255)

        gTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGGaa                                                                                 >  1:3872328/1‑93 (MQ=255)

        gTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCaa                                                                                 >  2:4470262/1‑93 (MQ=255)

          gAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCaaaa                                                                               <  1:269910/93‑1 (MQ=255)

          gAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCaaaa                                                                               <  1:3378288/93‑1 (MQ=255)

            aCAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACg                                                                             <  1:4038233/93‑1 (MQ=255)

              aagAAAACCCACCGGCAGCTACGCATCGGCTGTTGAGTGTTCAGACTGGCGCACGGGCGTGC‑GAGCAAATTGTTTC‑GCAAGCAGCGAAACGCa                                                                           <  2:732170/91‑1 (MQ=255)

               ggAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAg                                                                          <  1:3438604/93‑1 (MQ=255)

               ggAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAg                                                                          <  1:3225860/93‑1 (MQ=255)

               ggAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAg                                                                          <  1:2805914/93‑1 (MQ=255)

                gAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGt                                                                         <  1:1214976/93‑1 (MQ=255)

                 aaaaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGGAACATGTTTT‑GTAAAGAGCAAAACGCAGTg                                                                        >  1:3195368/1‑93 (MQ=255)

                 aaaaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTg                                                                        <  2:2691550/93‑1 (MQ=255)

                   aaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCCAATTGTTTC‑GCAAACAGCAAAACGCAGTGcc                                                                      >  1:22067/1‑93 (MQ=255)

                   aaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGcc                                                                      >  1:1274520/1‑93 (MQ=255)

                     cTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGa                                                                    <  1:2297436/93‑1 (MQ=255)

                      tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTT‑GCCAGCAGCAAAACGCAGTGCCGAt                                                                   >  2:1417216/1‑93 (MQ=255)

                        gctggCAGCTCCGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATgg                                                                 <  2:2805621/90‑1 (MQ=255)

                        cccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATgg                                                                 <  1:866627/92‑1 (MQ=255)

                        cccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATgg                                                                 <  2:1286749/92‑1 (MQ=255)

                        aCCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATgg                                                                 <  1:1524041/93‑1 (MQ=255)

                         ccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGG‑GGGCAAATTGTTTT‑GCAAGCAGCAAAACGCAGTGGCGATggg                                                                >  2:1640396/1‑92 (MQ=255)

                         ccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTT‑GCAAGCAGCAAAAAGCAGTGCCGATggg                                                                >  2:4138485/1‑92 (MQ=255)

                          cGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGACGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCg                                                               <  2:4640549/93‑1 (MQ=255)

                           ggCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGGAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCgg                                                              >  2:3551857/1‑92 (MQ=255)

                             cAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAgc                                                            <  2:303819/93‑1 (MQ=255)

                              aGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCCATGGCGAgct                                                           >  2:3184001/1‑93 (MQ=255)

                               gCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCCGTGCCGATGGCGAgctg                                                          >  1:707997/1‑93 (MQ=255)

                                cTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAgctgc                                                         <  1:3230981/93‑1 (MQ=255)

                                cTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAgctgc                                                         <  2:247949/93‑1 (MQ=255)

                                cTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAgctgc                                                         <  1:1471197/93‑1 (MQ=255)

                                 tACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAgctgct                                                        <  1:1140134/93‑1 (MQ=255)

                                   ggcgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑TGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGt                                                      <  1:2656660/92‑1 (MQ=255)

                                     cgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTa                                                    <  1:4495365/93‑1 (MQ=255)

                                     cgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTa                                                    <  1:311136/93‑1 (MQ=255)

                                      gTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAg                                                   <  2:229377/93‑1 (MQ=255)

                                      gTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAg                                                   >  1:2172422/1‑93 (MQ=255)

                                       tCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGGAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGc                                                  >  1:5023/1‑93 (MQ=255)

                                       tCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGc                                                  <  1:2563544/93‑1 (MQ=255)

                                         ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTa                                                <  1:850210/93‑1 (MQ=255)

                                         ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTa                                                >  1:997033/1‑93 (MQ=255)

                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCCAATTGGTTA‑CCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              >  2:3915240/1‑93 (MQ=255)

                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  1:1219005/93‑1 (MQ=255)

                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              >  2:1308273/1‑93 (MQ=255)

                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  2:2769766/93‑1 (MQ=255)

                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGACAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  2:1508277/93‑1 (MQ=255)

                                            tGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAccc                                             >  2:3164409/1‑93 (MQ=255)

                                               ttAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCa                                          >  2:3083621/1‑93 (MQ=255)

                                               ttAGTTTTCAGGCTGGCGCACTGGCGGGC‑CGGCAAATTGATTCGAAAACCACAAAACCGCCGGCCAGAT‑GCGGGCTGCTGTTAGCTACCCGCa                                          >  2:3135342/1‑93 (MQ=255)

                                                tAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCaa                                         <  2:3717514/93‑1 (MQ=255)

                                                  gTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGGAGTGCCGATTGCGAGCTGCTGTTAGTTACCCGCAAcc                                       >  1:3557420/1‑93 (MQ=255)

                                                  gTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAAcc                                       <  1:4314166/93‑1 (MQ=255)

                                                     ttCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCt                                    <  1:2825624/93‑1 (MQ=255)

                                                      tCAGGCTGGCGCACAGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGCGCCGCCGGTTGCTACCCGGACCCGCtt                                   >  2:4283109/1‑93 (MQ=255)

                                                          gCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               <  1:465137/93‑1 (MQ=255)

                                                          gCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               <  1:2465331/93‑1 (MQ=255)

                                                          gCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               >  1:2736957/1‑93 (MQ=255)

                                                            tGGCGCAGTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcag                             >  2:4608586/1‑93 (MQ=255)

                                                            tGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcag                             <  1:4098366/93‑1 (MQ=255)

                                                               cgcACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGt                          <  2:2899915/93‑1 (MQ=255)

                                                               cgcACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGt                          <  2:293550/93‑1 (MQ=255)

                                                                gcACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGtt                         <  2:3728899/93‑1 (MQ=255)

                                                                gcACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGtt                         <  1:636751/93‑1 (MQ=255)

                                                                gcACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGtt                         <  2:1230295/93‑1 (MQ=255)

                                                                 cACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCACTGCCGATGGCGAGCTGCCGGTAGGTACCCGCAACCGCCTGAGCAGCGTtg                        >  2:3607591/1‑93 (MQ=255)

                                                                    tGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACGCGCAACCGCTTAAGCAGCGTTGTGc                     >  2:3512950/1‑93 (MQ=255)

                                                                    tGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGc                     >  2:3711151/1‑93 (MQ=255)

                                                                    tGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGc                     <  1:3790625/93‑1 (MQ=255)

                                                                       cgggc‑gggCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGc                  <  1:2194222/93‑1 (MQ=255)

                                                                          gc‑gggcAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGt               <  1:776362/93‑1 (MQ=255)

                                                                           c‑gggcAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTc              <  2:2107240/93‑1 (MQ=255)

                                                                              ggcAAATTTTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGc            <  2:2585556/93‑1 (MQ=255)

                                                                                cAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCt          >  1:3280462/1‑93 (MQ=255)

                                                                                cAAAGTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCt          <  2:1527786/93‑1 (MQ=255)

                                                                                 aaaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTg         >  2:12248/1‑93 (MQ=255)

                                                                                 aaaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTg         >  2:1877904/1‑93 (MQ=255)

                                                                                  aaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  1:53640/93‑1 (MQ=255)

                                                                                  aaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  1:3602996/93‑1 (MQ=255)

                                                                                  aaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        >  1:3658048/1‑93 (MQ=255)

                                                                                  aaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        >  2:1720959/1‑93 (MQ=255)

                                                                                  aaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCCCAAACGCTTAAGCAGCGTTGTGCGGCTGTCGCCTGa        >  1:3111314/1‑93 (MQ=255)

                                                                                   aTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAt       <  1:1914282/93‑1 (MQ=255)

                                                                                   aTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAt       <  1:2036828/93‑1 (MQ=255)

                                                                                    ttGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATg      <  1:3445648/93‑1 (MQ=255)

                                                                                      gTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    >  2:4514465/1‑93 (MQ=255)

                                                                                      gTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  2:2848567/93‑1 (MQ=255)

                                                                                      gTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    >  2:1909346/1‑93 (MQ=255)

                                                                                        ttC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTg  <  1:3272409/93‑1 (MQ=255)

                                                                                        ttC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTg  <  1:2560435/93‑1 (MQ=255)

GCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑ACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208241‑2208420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`