BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,210,124	T→C	E31E (GAA→GAG)	yehS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,210,124	0	T	C	99.3%	436.6 / NA	139	E31E (GAA→GAG)	yehS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (77/61); minor base G (0/1); total (77/62)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GAGGAATGACGACAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAG  >  NC_000913/2210034‑2210213

gAGGAATGACGACAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTc                                                                                         >  2:2426196/1‑93 (MQ=255)

 aGGAATGACGACAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCg                                                                                        >  1:3128604/1‑93 (MQ=255)

  ggAATGACGACAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGa                                                                                       <  1:51819/93‑1 (MQ=255)

    aaTGACGATAAAACAATGTTCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACa                                                                                     >  2:4565624/1‑93 (MQ=255)

    aaTGACGACAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACa                                                                                     >  1:3143399/1‑93 (MQ=255)

     aTGACGACAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACAt                                                                                    <  1:3538461/93‑1 (MQ=255)

     aTGACGACAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACAt                                                                                    >  2:1985096/1‑93 (MQ=255)

     aTGACGACAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGCCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACAt                                                                                    >  1:2042217/1‑93 (MQ=255)

       gacgacAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTa                                                                                  <  1:2447030/93‑1 (MQ=255)

       gacgacAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTa                                                                                  <  1:745035/93‑1 (MQ=255)

       gacgacAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTa                                                                                  <  1:1628859/93‑1 (MQ=255)

         cgacAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTAcc                                                                                <  2:1583417/93‑1 (MQ=255)

          gacAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTAccc                                                                               >  1:1119879/1‑93 (MQ=255)

           acAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCa                                                                              >  1:2326829/1‑93 (MQ=255)

            cAAAACAATGTCCGGACAACGCTGAAACCCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAg                                                                             <  2:3309131/93‑1 (MQ=255)

              aaaCAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAgcg                                                                           >  1:2527134/1‑93 (MQ=255)

                aCAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGcc                                                                         >  2:2683329/1‑93 (MQ=255)

                aCAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGcc                                                                         >  1:3945163/1‑93 (MQ=255)

                 cAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCa                                                                        <  1:2127004/93‑1 (MQ=255)

                 cAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCa                                                                        <  2:706301/93‑1 (MQ=255)

                  aaTGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAg                                                                       >  2:4523782/1‑93 (MQ=255)

                      tCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATa                                                                   <  2:488942/93‑1 (MQ=255)

                      tCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATa                                                                   <  2:2950604/93‑1 (MQ=255)

                      tCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATa                                                                   <  2:939981/93‑1 (MQ=255)

                        cGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACg                                                                 >  1:1450049/1‑93 (MQ=255)

                          gACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCa                                                               >  1:2834805/1‑93 (MQ=255)

                          gACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCa                                                               >  1:4451384/1‑93 (MQ=255)

                          gACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCa                                                               <  1:1920802/93‑1 (MQ=255)

                            cAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCAcc                                                             >  1:2790767/1‑93 (MQ=255)

                            cAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCAcc                                                             <  2:191101/93‑1 (MQ=255)

                            cAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACACAGCGCCAGAATACGCAcc                                                             >  1:1352092/1‑93 (MQ=255)

                             aaCGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCa                                                            >  2:904697/1‑93 (MQ=255)

                             aaCGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCa                                                            >  1:915646/1‑93 (MQ=255)

                             aaCGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCa                                                            <  1:3919469/93‑1 (MQ=255)

                              aCGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAg                                                           <  1:1960314/93‑1 (MQ=255)

                              aCGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAg                                                           >  2:1464324/1‑93 (MQ=255)

                                gCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGt                                                         >  1:297051/1‑93 (MQ=255)

                                  tGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCa                                                       >  2:1419439/1‑93 (MQ=255)

                                    aaaaCCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCatt                                                     <  1:1296094/93‑1 (MQ=255)

                                     aaccccTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCatta                                                    <  1:5270/90‑1 (MQ=255)

                                     aaaCCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCatta                                                    <  1:3801486/93‑1 (MQ=255)

                                      accccTCTTCGTCTTCTTTACGTACCCAGTCGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCattat                                                   <  2:117064/91‑1 (MQ=255)

                                      aaCCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCattat                                                   <  1:1569506/93‑1 (MQ=255)

                                      aaCCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCattat                                                   >  2:4419238/1‑93 (MQ=255)

                                       aCCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCattatt                                                  <  2:2225115/93‑1 (MQ=255)

                                        cccTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCattatta                                                 >  1:2184041/1‑93 (MQ=255)

                                         ccTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCattattat                                                >  1:1036269/1‑93 (MQ=255)

                                         ccTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCattattat                                                >  1:2318216/1‑93 (MQ=255)

                                          ctctTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCattattatt                                               >  1:1049955/1‑93 (MQ=255)

                                          ctctTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCattattatt                                               >  2:3698077/1‑93 (MQ=255)

                                          ctctTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCattattatt                                               <  1:1505665/93‑1 (MQ=255)

                                          ctctTCGTCTTCTTTACGTAGCCAGA‑GGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTg                                              >  1:2277292/1‑93 (MQ=255)

                                           tctTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTg                                              >  2:2313206/1‑93 (MQ=255)

                                           tctTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTg                                              >  1:3367386/1‑93 (MQ=255)

                                           tctTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTg                                              >  2:4196742/1‑93 (MQ=255)

                                             ttCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGc                                            <  1:3056384/93‑1 (MQ=255)

                                              tCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCt                                           >  1:4170227/1‑93 (MQ=255)

                                              tCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCt                                           <  1:4080452/93‑1 (MQ=255)

                                              tCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCt                                           <  2:107741/93‑1 (MQ=255)

                                               cGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCtt                                          <  2:3231522/93‑1 (MQ=255)

                                                 tcttctTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTc                                        <  1:1350184/93‑1 (MQ=255)

                                                 tcttctTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTc                                        >  1:147845/1‑93 (MQ=255)

                                                 tcttctTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTc                                        >  1:3543201/1‑93 (MQ=255)

                                                   ttcttTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCaa                                      >  1:1177706/1‑93 (MQ=255)

                                                   ttcttTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCaa                                      <  2:272501/93‑1 (MQ=255)

                                                   ttcttTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCaa                                      >  1:258278/1‑93 (MQ=255)

                                                   ttcttTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGCCATTATTATTGGCTTTCaa                                      >  2:1937788/1‑93 (MQ=255)

                                                     cttTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCaaaa                                    <  2:1063158/93‑1 (MQ=255)

                                                      tttACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAAt                                   >  1:406846/1‑93 (MQ=255)

                                                      tttACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAAt                                   >  1:4174678/1‑93 (MQ=255)

                                                        tACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGt                                 >  2:3389276/1‑93 (MQ=255)

                                                         aCGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGCCATTATTATTGGCTTTCAAAATGTa                                >  1:37740/1‑93 (MQ=255)

                                                          cGTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAg                               <  1:1703528/93‑1 (MQ=255)

                                                           gTAGCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAgc                              <  2:33959/93‑1 (MQ=255)

                                                              gCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCa                           >  1:1243036/1‑93 (MQ=255)

                                                              gCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCa                           >  1:2547943/1‑93 (MQ=255)

                                                              gCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCa                           <  1:3779278/93‑1 (MQ=255)

                                                              gCCAGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCa                           >  2:2410270/1‑93 (MQ=255)

                                                                 aGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGc                        <  2:4397752/93‑1 (MQ=255)

                                                                 aGACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGc                        >  2:3729054/1‑93 (MQ=255)

                                                                  gACGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCt                       >  2:3573055/1‑93 (MQ=255)

                                                                    cGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTgc                     <  2:4046891/93‑1 (MQ=255)

                                                                    cGGCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTgc                     >  1:4233224/1‑93 (MQ=255)

                                                                      gCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTgcgc                   >  2:3847735/1‑93 (MQ=255)

                                                                      gCGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTgcgc                   >  2:2414974/1‑93 (MQ=255)

                                                                       cGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCa                  >  1:4073596/1‑93 (MQ=255)

                                                                       cGATCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCa                  <  2:558953/93‑1 (MQ=255)

                                                                         aTCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGa                >  2:3513122/1‑93 (MQ=255)

                                                                         aTCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGa                >  1:2193156/1‑93 (MQ=255)

                                                                         aTCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGa                >  2:3206251/1‑93 (MQ=255)

                                                                          tCTGTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGaa               >  1:4246847/1‑93 (MQ=255)

                                                                             gTTCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAatat            >  2:1217509/1‑93 (MQ=255)

                                                                              ttCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATc           <  1:1917068/93‑1 (MQ=255)

                                                                              ttCCGCGGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGAAGAATATc           <  2:4577560/93‑1 (MQ=255)

                                                                                 cgcgGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGtt        <  2:1592300/93‑1 (MQ=255)

                                                                                  gcgGTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTa       >  2:1433006/1‑93 (MQ=255)

                                                                                    ggTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACt     <  1:2421931/93‑1 (MQ=255)

                                                                                    ggTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACt     <  1:3094523/93‑1 (MQ=255)

                                                                                     gTGGCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACtt    >  2:1611153/1‑93 (MQ=255)

                                                                                       ggCCTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAg  >  1:738785/1‑93 (MQ=255)

GAGGAATGACGACAAAACAATGTCCGGACAACGCTGAAAACCCTCTTCGTCTTCTTTACGTAGCCAGACGGCGATCTGTTCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAG  >  NC_000913/2210034‑2210213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`