BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	230,556	A→G	M109T (ATG→ACG)	yafC ←	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	230,556	0	A	G	100.0%	569.7 / NA	179	M109T (ATG→ACG)	yafC	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (85/94); total (85/94)

Only 100 of 184 total aligned reads displayed.

TTTCTTTCGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGATTCTGCC  >  NC_000913/230466‑230645

tttctttcGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTc                                                                                         <  2:4590002/93‑1 (MQ=255)

   ctttcGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGa                                                                                      >  1:2267251/1‑93 (MQ=255)

     ttcGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGaaa                                                                                    <  2:1946375/93‑1 (MQ=255)

     ttcGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGATTAATTAACGGCGTCAGaaa                                                                                    >  2:2251174/1‑93 (MQ=255)

      tcGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAg                                                                                   >  1:478816/1‑93 (MQ=255)

       cGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGt                                                                                  <  2:2594990/93‑1 (MQ=255)

        gATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTg                                                                                 >  1:3908285/1‑93 (MQ=255)

        gATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTg                                                                                 >  1:2704977/1‑93 (MQ=255)

        gATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAAAGGCGTCAGAAAgca                                                                                 >  1:2698956/1‑91 (MQ=255)

          tCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTgca                                                                               <  1:4592834/93‑1 (MQ=255)

          tCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTgca                                                                               >  2:2423940/1‑93 (MQ=255)

           cAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACAGCGTCAGAAAGTgcag                                                                              >  1:1135339/1‑93 (MQ=255)

             aaTTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTgcagca                                                                            >  2:2717333/1‑93 (MQ=255)

             aaTTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTgcagca                                                                            >  2:3405691/1‑93 (MQ=255)

             aaTTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTgcagca                                                                            <  2:2030986/93‑1 (MQ=255)

              aTTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGcac                                                                           >  1:3349324/1‑93 (MQ=255)

               ttAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGcacc                                                                          <  2:3201853/93‑1 (MQ=255)

                 aataatCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGcaccac                                                                        >  2:139510/1‑93 (MQ=255)

                 aataatCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGcaccac                                                                        >  1:332175/1‑93 (MQ=255)

                  ataatCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACt                                                                       >  1:3274132/1‑93 (MQ=255)

                   taatCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTg                                                                      <  2:262899/93‑1 (MQ=255)

                   taatCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTg                                                                      >  2:1544459/1‑93 (MQ=255)

                   aaatCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTg                                                                      <  2:3962177/92‑1 (MQ=255)

                     atCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGa                                                                    >  1:4306595/1‑93 (MQ=255)

                     atCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGa                                                                    >  2:759659/1‑93 (MQ=255)

                     atCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGa                                                                    >  1:2448573/1‑93 (MQ=255)

                     atCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGa                                                                    >  1:2190319/1‑93 (MQ=255)

                       cGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGt                                                                  <  1:3200885/93‑1 (MQ=255)

                        gTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGtt                                                                 <  2:2181765/93‑1 (MQ=255)

                        gTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGtt                                                                 <  1:3555181/93‑1 (MQ=255)

                         tttCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTg                                                                >  1:893097/1‑93 (MQ=255)

                          ttCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGc                                                               <  2:3636005/93‑1 (MQ=255)

                          ttCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGc                                                               <  1:1006947/93‑1 (MQ=255)

                           tCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCg                                                              <  1:4206723/93‑1 (MQ=255)

                             ggaggaGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGc                                                            >  1:4081008/1‑93 (MQ=255)

                                ggagACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATc                                                         <  2:3970957/93‑1 (MQ=255)

                                  agaCTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGa                                                       <  1:676120/93‑1 (MQ=255)

                                   gaCTAGCGACAAAGTGCCTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCCCCACTGGAGTTGCGGCATCGAt                                                      <  2:86202/93‑1 (MQ=255)

                                   gaCTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGAt                                                      <  1:2782850/93‑1 (MQ=255)

                                   gaCTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGAt                                                      >  2:2822303/1‑93 (MQ=255)

                                   gaCTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGAt                                                      >  1:750321/1‑93 (MQ=255)

                                    aCTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATc                                                     >  1:3490169/1‑93 (MQ=255)

                                     cTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATcc                                                    <  2:3630152/93‑1 (MQ=255)

                                     cTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATcc                                                    <  2:1852641/93‑1 (MQ=255)

                                      tAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCg                                                   >  1:4219853/1‑93 (MQ=255)

                                        gCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTa                                                 >  1:1954358/1‑93 (MQ=255)

                                         cGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTaa                                                <  1:3405021/93‑1 (MQ=255)

                                         cGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTaa                                                <  2:4545274/93‑1 (MQ=255)

                                           aCAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa                                              >  1:842362/1‑93 (MQ=255)

                                            cAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCACCACCACTGGAGTTGCGGCATCGATCCGTAACAg                                             >  2:4295531/1‑93 (MQ=255)

                                             aaaGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGt                                            >  1:4506006/1‑93 (MQ=255)

                                              aaGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTc                                           >  2:3320071/1‑93 (MQ=255)

                                              aaGTGACTTCCGAATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTc                                           <  1:4403274/93‑1 (MQ=255)

                                              aaGTGACTGCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTc                                           <  1:1543893/93‑1 (MQ=255)

                                               aGTGACTTCCGGATATCGTTCACGTAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTcc                                          <  2:3520879/93‑1 (MQ=255)

                                                gTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCa                                         <  2:4668092/93‑1 (MQ=255)

                                                     ttCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCgg                                    <  2:3421204/93‑1 (MQ=255)

                                                         ggATAGCGTTCACGGAAAGCCTTAATTCACGGCGTCAGAAAGTGCAGCCCCGCTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTa                                <  2:4139127/93‑1 (MQ=255)

                                                          ggtAGCGTTCACGGAAAGGCTTGATTAAGGGGGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCAGCGATCCGTAACAGGCCACGCGgtgtgt                               >  2:3269665/3‑91 (MQ=255)

                                                          gATAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTAt                               >  2:2114964/1‑93 (MQ=255)

                                                           aTAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTAtt                              >  1:306853/1‑93 (MQ=255)

                                                           aTAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTAtt                              <  1:262855/93‑1 (MQ=255)

                                                            tAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTa                             >  1:666114/1‑93 (MQ=255)

                                                            tAGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTa                             <  1:2154080/93‑1 (MQ=255)

                                                             aGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTAc                            <  1:922215/93‑1 (MQ=255)

                                                             aGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTAc                            <  2:3741663/93‑1 (MQ=255)

                                                             aGCGTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTAc                            <  1:3670073/93‑1 (MQ=255)

                                                                gTTCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTAcgcg                         >  1:1014008/1‑93 (MQ=255)

                                                                 ttCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGt                        >  1:1563737/1‑93 (MQ=255)

                                                                 ttCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGt                        >  1:173872/1‑93 (MQ=255)

                                                                  tCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGtc                       <  2:681870/93‑1 (MQ=255)

                                                                  tCACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGtc                       >  1:3948350/1‑93 (MQ=255)

                                                                   cACGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGtct                      >  2:1857693/1‑93 (MQ=255)

                                                                     cGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTcc                    <  2:1483517/93‑1 (MQ=255)

                                                                     cGGAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTcc                    <  2:2614019/93‑1 (MQ=255)

                                                                      ggAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCa                   <  1:4168213/93‑1 (MQ=255)

                                                                      ggAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCa                   >  1:3830559/1‑93 (MQ=255)

                                                                       gAAAGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCAt                  <  2:521507/93‑1 (MQ=255)

                                                                         aaGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATaa                >  1:233719/1‑93 (MQ=255)

                                                                          aGGCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAAt               >  2:2154378/1‑93 (MQ=255)

                                                                           ggCTTAATTAACCGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAAtt              <  2:2321907/93‑1 (MQ=255)

                                                                            gCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAAttt             >  1:548026/1‑93 (MQ=255)

                                                                            gCTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAAttt             <  2:363604/93‑1 (MQ=255)

                                                                             cTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTc            <  2:4169440/93‑1 (MQ=255)

                                                                             cTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTc            >  1:1879315/1‑93 (MQ=255)

                                                                             cTTAATTAACGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTc            <  2:2499478/93‑1 (MQ=255)

                                                                              ttaattaaCGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCt           <  1:2854474/93‑1 (MQ=255)

                                                                               taattaaCGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTg          >  1:1204291/1‑93 (MQ=255)

                                                                                aattaaCGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGa         <  1:1979667/93‑1 (MQ=255)

                                                                                aattaaCGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGa         <  1:3146518/93‑1 (MQ=255)

                                                                                 attaaCGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGAt        >  2:3485138/1‑93 (MQ=255)

                                                                                  ttaaCGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGAtt       <  2:1174293/93‑1 (MQ=255)

                                                                                   taaCGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGATTc      <  1:1132677/93‑1 (MQ=255)

                                                                                    aaCGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGATTCt     <  1:3460449/93‑1 (MQ=255)

                                                                                    aaCGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGATTCt     <  1:1324210/93‑1 (MQ=255)

                                                                                     aCGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGATTCTg    >  2:4173553/1‑93 (MQ=255)

                                                                                     aCGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGATTCTg    <  2:915468/93‑1 (MQ=255)

                                                                                      cGGCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGATTCTGc   <  2:3051056/93‑1 (MQ=255)

                                                                                       ggCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGATTCTGcc  <  2:4459608/93‑1 (MQ=255)

                                                                                       ggCGTCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGATTCTGcc  <  2:9813/93‑1 (MQ=255)

TTTCTTTCGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCGGTGTATTACGCGTCTCCATAATTTCTGATTCTGCC  >  NC_000913/230466‑230645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`