BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,225,197	T→C	T32A (ACC→GCC)	yohF ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,225,197	0	T	C	99.2%	410.5 / NA	130	T32A (ACC→GCC)	yohF	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (63/66); minor base G (1/0); total (64/66)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

CGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTG  >  NC_000913/2225107‑2225286

cGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCaa                                                                                         >  1:761916/1‑93 (MQ=255)

cGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCaa                                                                                         <  1:1346895/93‑1 (MQ=255)

 gagaTCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAAt                                                                                        >  1:3722383/1‑93 (MQ=255)

  agaTCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATa                                                                                       <  1:3380680/93‑1 (MQ=255)

     tCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCa                                                                                    >  2:2742881/1‑93 (MQ=255)

       cAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAat                                                                                  <  2:138340/93‑1 (MQ=255)

        cgCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAata                                                                                 <  2:4216314/92‑1 (MQ=255)

        aGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAata                                                                                 <  1:3739709/93‑1 (MQ=255)

          cTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATc                                                                               <  1:3148841/93‑1 (MQ=255)

          cTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATc                                                                               <  1:2031775/93‑1 (MQ=255)

            gCACGATCTCCGCACGTACGCCGTGGCTAGCTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCaa                                                                             <  1:2338364/93‑1 (MQ=255)

            gCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCaa                                                                             <  1:3982895/93‑1 (MQ=255)

            gCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCaa                                                                             <  2:3276380/93‑1 (MQ=255)

             cACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCaaa                                                                            <  2:1536453/93‑1 (MQ=255)

              aCGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAAc                                                                           <  1:289681/93‑1 (MQ=255)

              aCGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAAc                                                                           <  2:2247748/93‑1 (MQ=255)

              aCGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAAc                                                                           >  1:3473710/1‑93 (MQ=255)

              aCGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAAc                                                                           >  1:2085359/1‑93 (MQ=255)

               cGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAAcc                                                                          >  2:3425219/1‑93 (MQ=255)

               cGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAAcc                                                                          <  2:1332905/93‑1 (MQ=255)

                gATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAAccc                                                                         >  1:3932136/1‑93 (MQ=255)

                  tctcCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCct                                                                       <  1:3314826/93‑1 (MQ=255)

                  tctcCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCct                                                                       >  1:639859/1‑93 (MQ=255)

                  tctaCGCGCGTACGGCGTGGCTAACTCCCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCct                                                                       <  1:178308/93‑1 (MQ=255)

                    tcCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCctgc                                                                     <  1:238691/93‑1 (MQ=255)

                    tcCGCACGTACGCCGTGACTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCctgc                                                                     <  2:556925/93‑1 (MQ=255)

                       gCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCtgctgc                                                                  <  1:491570/93‑1 (MQ=255)

                       gCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCtgctgc                                                                  >  2:1812265/1‑93 (MQ=255)

                        cACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTgcg                                                                 >  1:3178033/1‑93 (MQ=255)

                         aCGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTgcgc                                                                >  2:3050429/1‑93 (MQ=255)

                         aCGTACGCCGTGGATCACTACCTCACGCGCGGCATCTTTTTCCCCTTCTTCATCTGAGTGCCAGGCAATACCAACATCAAACCCCTACTccgc                                                                >  2:3468163/1‑93 (MQ=255)

                           gTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCa                                                              >  2:3030636/1‑93 (MQ=255)

                           gTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCa                                                              >  1:4079257/1‑93 (MQ=255)

                           gTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAAACCCTGCTGCGCCa                                                              >  2:429939/1‑93 (MQ=255)

                               gCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGtaa                                                          <  1:2476883/93‑1 (MQ=255)

                               gCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGtaa                                                          <  1:3478939/93‑1 (MQ=255)

                                 cGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGtaata                                                        <  2:4129096/93‑1 (MQ=255)

                                  gTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGtaataa                                                       <  2:3444614/93‑1 (MQ=255)

                                  gTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGtaataa                                                       >  2:1382157/1‑93 (MQ=255)

                                   tGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAAc                                                      >  1:465674/1‑93 (MQ=255)

                                   tGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAAc                                                      >  1:1060656/1‑93 (MQ=255)

                                   tGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAAc                                                      <  1:1569521/93‑1 (MQ=255)

                                      cTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAAcgcg                                                   >  1:1652283/1‑93 (MQ=255)

                                       tAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACgcgc                                                  >  2:381434/1‑93 (MQ=255)

                                       tAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACgcgc                                                  >  1:4237187/1‑93 (MQ=255)

                                        aaCTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCa                                                 <  1:2087732/93‑1 (MQ=255)

                                        aaCTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCa                                                 <  2:2960559/93‑1 (MQ=255)

                                        aaCTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACACCTGCTGCGCCAGTCATAACGCGCa                                                 >  2:2583514/1‑93 (MQ=255)

                                           tACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCActc                                              <  2:4496686/93‑1 (MQ=255)

                                           tACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCActc                                              >  2:930499/1‑93 (MQ=255)

                                           tACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCActc                                              >  1:1817924/1‑93 (MQ=255)

                                           tACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCActc                                              >  1:4474341/1‑93 (MQ=255)

                                             ccTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCtt                                            <  2:1267208/93‑1 (MQ=255)

                                              cTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCttt                                           <  2:720599/93‑1 (MQ=255)

                                              cTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCttt                                           <  1:3921397/93‑1 (MQ=255)

                                                cACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGc                                         <  1:560351/93‑1 (MQ=255)

                                                cACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGc                                         <  1:4255095/93‑1 (MQ=255)

                                                 aCGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGcc                                        <  1:4034870/93‑1 (MQ=255)

                                                     gcgGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATc                                    >  1:610641/1‑93 (MQ=255)

                                                     gcgGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATc                                    >  2:2132185/1‑93 (MQ=255)

                                                       ggTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGGAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATccc                                  >  2:1275862/1‑93 (MQ=255)

                                                        gTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATcccc                                 <  1:918086/93‑1 (MQ=255)

                                                        gTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATcccc                                 >  1:3441661/1‑93 (MQ=255)

                                                          aTCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGa                               >  2:254512/1‑93 (MQ=255)

                                                          aTCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGa                               >  2:2615652/1‑93 (MQ=255)

                                                          aTCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGa                               >  2:3798670/1‑93 (MQ=255)

                                                           tCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGac                              >  2:3285969/1‑92 (MQ=255)

                                                           tCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGaa                              <  1:2223876/93‑1 (MQ=255)

                                                           tCTTTTGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGaa                              <  1:4293039/93‑1 (MQ=255)

                                                            cTTTTGCCTTTTCTTCATCTGAGTGCCAGACATTCCCAATAATAAACCCCAGCTGCGCCAGGATTAACGCGCGCTCTTCGCCGATCACCGAAt                             <  2:509518/93‑1 (MQ=255)

                                                             ttttGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATc                            <  1:2998417/93‑1 (MQ=255)

                                                             ttttGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATc                            >  1:3478388/1‑93 (MQ=255)

                                                                tGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGa                         <  1:3822636/93‑1 (MQ=255)

                                                                tGCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGa                         <  2:2968670/93‑1 (MQ=255)

                                                                 gCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAg                        >  1:1726344/1‑93 (MQ=255)

                                                                 gCCCCTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAg                        <  1:1357102/93‑1 (MQ=255)

                                                                  ccccTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAgg                       >  2:1610901/1‑93 (MQ=255)

                                                                   cccTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAAGc                      >  1:1858123/1‑93 (MQ=255)

                                                                    ccTTCTTCATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCg                     >  2:58906/1‑93 (MQ=255)

                                                                      ttcttcATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCgga                   >  1:1122538/1‑92 (MQ=255)

                                                                      ttcttcATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGt                   <  1:1026505/93‑1 (MQ=255)

                                                                       tcttcATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGta                  <  1:2193025/93‑1 (MQ=255)

                                                                        cttcATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGtaa                 <  1:3648135/93‑1 (MQ=255)

                                                                        cttcATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCAAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGtaa                 <  2:4080357/93‑1 (MQ=255)

                                                                         ttcATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGtaat                >  2:1520392/1‑93 (MQ=255)

                                                                         ttcATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGtaat                >  1:3220514/1‑93 (MQ=255)

                                                                           cATCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGtaataa              >  1:1404898/1‑93 (MQ=255)

                                                                            aTCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTaataat             <  2:4665653/93‑1 (MQ=255)

                                                                             tCTGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATc            <  2:2731357/93‑1 (MQ=255)

                                                                               tGAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGc          >  1:1012836/1‑93 (MQ=255)

                                                                                gAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCa         >  2:1212308/1‑93 (MQ=255)

                                                                                gAGTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCa         >  2:2586677/1‑93 (MQ=255)

                                                                                  gTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAAc       <  2:43924/93‑1 (MQ=255)

                                                                                  gTGCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAAAGCGCACTCTTTGTCGATCACCGAATCGGAGGCGGTAATAATAGCAAc       >  2:499995/1‑93 (MQ=255)

                                                                                    gCCAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCt     >  1:912679/1‑93 (MQ=255)

                                                                                     ccAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCtg    <  1:2778614/93‑1 (MQ=255)

                                                                                      cAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCtgt   >  1:2741353/1‑93 (MQ=255)

                                                                                      cAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCtgt   <  1:2762158/93‑1 (MQ=255)

                                                                                      cAGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCtgt   >  1:3051935/1‑93 (MQ=255)

                                                                                       aGGCAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCtgtg  >  1:2200566/1‑93 (MQ=255)

CGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTG  >  NC_000913/2225107‑2225286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`