BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,741	T→C	D76G (GAC→GGC)	yejK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,741	0	T	C	99.2%	396.1 / NA	128	D76G (GAC→GGC)	yejK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (66/61); minor base G (1/0); total (67/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

AAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAG  >  NC_000913/2281652‑2281830

  aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCTCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                      <  1:351311/93‑1 (MQ=255)

  aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                      >  1:3738710/1‑93 (MQ=255)

  aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                      <  1:1737305/93‑1 (MQ=255)

  aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGCTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                      >  2:2478304/1‑93 (MQ=255)

   aCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCtt                                                                                     <  1:4051949/93‑1 (MQ=255)

   aCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCtt                                                                                     <  1:650691/93‑1 (MQ=255)

    cAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTc                                                                                    <  1:1970434/93‑1 (MQ=255)

      gCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGc                                                                                  >  2:2386364/1‑93 (MQ=255)

        aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGccc                                                                                <  2:2726331/93‑1 (MQ=255)

        aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGccc                                                                                >  2:447089/1‑93 (MQ=255)

         cGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCt                                                                               >  1:4155341/1‑93 (MQ=255)

          gAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTg                                                                              >  2:3516313/1‑93 (MQ=255)

           aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCTCTCTTCGCCCTgc                                                                             >  1:3833856/1‑92 (MQ=255)

           aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGa                                                                             >  1:123916/1‑93 (MQ=255)

           aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGa                                                                             >  1:3014498/1‑93 (MQ=255)

            aTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGAc                                                                            <  2:638002/93‑1 (MQ=255)

             tCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACg                                                                           >  1:2232167/1‑93 (MQ=255)

             tCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACg                                                                           >  1:3633458/1‑93 (MQ=255)

                  cGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTa                                                                      <  2:4628112/93‑1 (MQ=255)

                   gTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTaa                                                                     <  2:1752013/93‑1 (MQ=255)

                    tCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAg                                                                    <  1:3085213/93‑1 (MQ=255)

                     cAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgc                                                                   <  1:4587873/93‑1 (MQ=255)

                     cAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgc                                                                   >  2:1062596/1‑93 (MQ=255)

                       gCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgcgc                                                                 <  2:4344972/93‑1 (MQ=255)

                       gCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgcgc                                                                 >  1:1492245/1‑93 (MQ=255)

                        cAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                                >  1:478173/1‑93 (MQ=255)

                         aaaaGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAg                                                               <  1:2636654/93‑1 (MQ=255)

                          aaaGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGc                                                              >  1:1486189/1‑93 (MQ=255)

                            aGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGt                                                            >  2:3012146/1‑93 (MQ=255)

                            aGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGt                                                            <  2:496868/93‑1 (MQ=255)

                             ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           >  1:665705/1‑93 (MQ=255)

                             ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           >  1:2396852/1‑93 (MQ=255)

                             ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           >  2:2669065/1‑93 (MQ=255)

                             ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           >  1:4132186/1‑93 (MQ=255)

                             ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           <  1:946229/93‑1 (MQ=255)

                              gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                          >  2:404379/1‑93 (MQ=255)

                              gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                          <  2:3281431/93‑1 (MQ=255)

                              gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                          <  1:3202766/93‑1 (MQ=255)

                               atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         <  1:3666889/93‑1 (MQ=255)

                               atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         >  2:2540618/1‑93 (MQ=255)

                               atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         >  2:2951216/1‑93 (MQ=255)

                               atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         >  2:4157548/1‑93 (MQ=255)

                               atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         >  1:1062576/1‑93 (MQ=255)

                               atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCACTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         >  2:3307696/1‑93 (MQ=255)

                                tatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgc                                                        >  2:2650909/1‑93 (MQ=255)

                                tatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgc                                                        <  2:4653858/93‑1 (MQ=255)

                                tatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCATCGCCCTGACGCTGTAAGCGCAGCGTCTgc                                                        >  2:3469400/1‑93 (MQ=255)

                                 atTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcg                                                       >  1:1154261/1‑93 (MQ=255)

                                 atTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcg                                                       <  1:3977075/93‑1 (MQ=255)

                                    tCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCa                                                    <  1:697319/93‑1 (MQ=255)

                                      gCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGt                                                  >  1:3316732/1‑93 (MQ=255)

                                         aaTTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCa                                               <  2:1181436/93‑1 (MQ=255)

                                          aTTCGTCACGCAGGTGGCCGGTTGCCCCGCGGCTAAATGCCCGGAAGCCGTCTTCGCCCCAACGCTGTTCGCGCAGCCTCGGCGCCAGTTCat                                              >  2:666154/1‑92 (MQ=255)

                                          aTTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGGCCTCTTCGCCCTGACGATGTAAGCGCAGCGTCTGCGCCAGTTCaa                                              >  2:3100587/1‑92 (MQ=255)

                                                cACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCtt                                        >  1:3984162/1‑93 (MQ=255)

                                                cACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTCCGCCAGTTCACTCTCtt                                        >  1:2999664/1‑93 (MQ=255)

                                                  cGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCg                                      >  2:850188/1‑93 (MQ=255)

                                                   gCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGc                                     >  2:3439740/1‑93 (MQ=255)

                                                   gCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGc                                     <  1:1642170/93‑1 (MQ=255)

                                                   gCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGc                                     <  1:4597545/93‑1 (MQ=255)

                                                    cAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCt                                    <  2:2493448/93‑1 (MQ=255)

                                                     aGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTa                                   <  1:524763/93‑1 (MQ=255)

                                                      ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                  >  2:2098163/1‑93 (MQ=255)

                                                          gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                              <  1:2397571/93‑1 (MQ=255)

                                                          gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                              >  2:4446227/1‑93 (MQ=255)

                                                          gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                              <  1:2268203/93‑1 (MQ=255)

                                                           ccGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAg                             <  1:3785065/93‑1 (MQ=255)

                                                           ccGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAg                             <  2:1181078/93‑1 (MQ=255)

                                                            cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                            <  2:4226616/93‑1 (MQ=255)

                                                            cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                            <  2:852474/93‑1 (MQ=255)

                                                             ggTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGcc                           <  1:853657/93‑1 (MQ=255)

                                                              gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                          <  1:4050692/93‑1 (MQ=255)

                                                              gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                          >  1:953057/1‑93 (MQ=255)

                                                              gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                          <  2:2784393/93‑1 (MQ=255)

                                                                 gCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAACAAGCCGTaa                       <  2:4669844/93‑1 (MQ=255)

                                                                 gCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTaa                       <  1:4331600/93‑1 (MQ=255)

                                                                 gCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTaa                       <  1:3840292/93‑1 (MQ=255)

                                                                 gCCGCGCGGCTAAATGCCAGGAAGCCCTCTCCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCGGTTCCCTCTCTTCGCTAAACAAGCCGTaa                       <  2:2136382/93‑1 (MQ=255)

                                                                   cgcgcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGc                     >  2:177296/1‑93 (MQ=255)

                                                                     cgcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCtt                   <  2:2199027/93‑1 (MQ=255)

                                                                     cgcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCtt                   >  1:1155957/1‑93 (MQ=255)

                                                                        ggCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAt                <  2:2558071/93‑1 (MQ=255)

                                                                        ggCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAt                >  2:3938488/1‑93 (MQ=255)

                                                                          cTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAttt              >  2:788237/1‑93 (MQ=255)

                                                                           tAAATGCCAGGAGGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCTCTCTCTTCGCTAAACAAGCCGTAAGCTTTAtttt             <  2:2096598/93‑1 (MQ=255)

                                                                           tAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtttt             >  2:1256434/1‑93 (MQ=255)

                                                                            aaaTGCGCGGAAGCCCTCTTCCCCCTGACGCTTTAAGCGCAGCGTCTGCCCCAGTTCCCTCTCTCCGCTAAACAAGCCGTAAGCTTTAttttt            <  2:1689806/93‑1 (MQ=255)

                                                                            aaaTGCCAGGAACCCCCCTCCGACCTAACTTTGTAGTCGCAGCGTCTGCCCCAGTTCTCTCTTTTCGCTAAACAAGCCGTAAGCTTTAttttt            <  2:3576559/93‑1 (MQ=255)

                                                                            aaaCGCCAGGAGCCCCTCTTCGCCCTGACGCTGTAGGCGCGGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAttttt            <  2:1221701/93‑1 (MQ=255)

                                                                             aaTGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTg           >  1:4365330/1‑93 (MQ=255)

                                                                             aaTCCCAGGAAACCCTCTCCGCCCTGACGCTGTAAGCGAAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTg           <  2:3797782/93‑1 (MQ=255)

                                                                              aTGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTgg          >  1:2595133/1‑93 (MQ=255)

                                                                              aTGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGGTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTgg          >  2:2419913/1‑93 (MQ=255)

                                                                                gCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGgcg        >  2:735975/1‑93 (MQ=255)

                                                                                 ccAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGgcgc       >  1:1632442/1‑93 (MQ=255)

                                                                                   cggAAGCCCTCTCCGCCCTGACGCTGTAAGCGCGGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCta     <  1:812576/92‑1 (MQ=255)

                                                                                     gAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata   >  2:1363522/1‑93 (MQ=255)

                                                                                     gAAGCCCTCTCCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata   <  1:628697/93‑1 (MQ=255)

                                                                                      aaGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAg  >  1:1284950/1‑93 (MQ=255)

                                                                                      aaGCCCTCTCCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAg  <  1:3772374/93‑1 (MQ=255)

AAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAG  >  NC_000913/2281652‑2281830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`