BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,290,493	A→G	N164N (AAT→AAC)	dsbE ←	disulfide oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,290,493	0	A	G	98.4%	390.5 / ‑2.9	127	N164N (AAT→AAC)	dsbE	disulfide oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (58/67); total (59/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 139 total aligned reads displayed.

GCATCAGCACGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGATTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGACACCGAGATCCAGCC  >  NC_000913/2290403‑2290581

gcatcagcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGCCGCGCGGGtt                                                                                        >  2:853262/1‑93 (MQ=255)

 catcagcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTc                                                                                       <  1:2841199/93‑1 (MQ=255)

  atcagcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCa                                                                                      >  1:1764389/1‑93 (MQ=255)

   tcagcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGGTCTCTTCTTTCCAGACGCGAGGGCTCAg                                                                                     >  2:2827312/1‑93 (MQ=255)

   tcagcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAg                                                                                     >  2:1658139/1‑93 (MQ=255)

    cagcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGa                                                                                    <  1:1084591/93‑1 (MQ=255)

    cagcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGa                                                                                    <  1:3756372/93‑1 (MQ=255)

    cagcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGa                                                                                    <  1:2011587/93‑1 (MQ=255)

     agcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGAt                                                                                   <  2:3291453/93‑1 (MQ=255)

     agcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGAt                                                                                   <  1:460212/93‑1 (MQ=255)

     agcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGATTCAGAt                                                                                   >  1:341956/1‑93 (MQ=255)

     agcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGATTCAGAt                                                                                   >  1:1052529/1‑93 (MQ=255)

      gcaCGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATc                                                                                  >  1:2529224/1‑93 (MQ=255)

        aCGCCCAATAAAAACCTCATTGTGCGGCCTCCTCACTGTATTTCTCCCACAGCTGCTTGATTTCTTCTTTTCGGATGCGAGGGGTCAGATctc                                                                                >  2:3094957/1‑91 (MQ=255)

         cGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGcc                                                                               <  1:1525957/93‑1 (MQ=255)

            ccAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGc                                                                            <  2:4642603/93‑1 (MQ=255)

            ccAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGGTCAGATCGCCCGc                                                                            >  1:583696/1‑93 (MQ=255)

             cAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCa                                                                           >  2:627007/1‑93 (MQ=255)

             cAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGGTCAGATCGCCCGCa                                                                           >  2:1579548/1‑93 (MQ=255)

              aaTAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCAt                                                                          <  1:4224436/93‑1 (MQ=255)

              aaTAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCAt                                                                          <  1:3558427/93‑1 (MQ=255)

               aTAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATg                                                                         >  1:315086/1‑93 (MQ=255)

               aTAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTATTCTTCCCAGACGCGAGGGATCAGATCGCCCGCATg                                                                         >  2:4375905/1‑93 (MQ=255)

                tAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATgg                                                                        >  2:4652060/1‑93 (MQ=255)

                 aaaaaCCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGc                                                                       <  1:4114175/93‑1 (MQ=255)

                  aaaaCCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCg                                                                      >  1:1820550/1‑93 (MQ=255)

                   aaaCCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGa                                                                     >  1:2750856/1‑93 (MQ=255)

                     aCCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATa                                                                   <  2:1228712/93‑1 (MQ=255)

                     aCCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATa                                                                   <  2:2894014/93‑1 (MQ=255)

                     aCCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATa                                                                   <  1:2593582/93‑1 (MQ=255)

                     aCCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATa                                                                   >  2:4128321/1‑93 (MQ=255)

                      ccTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGGTCAGCTCGCCCGCATGGCGATAg                                                                  >  2:262544/1‑93 (MQ=255)

                       cTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGc                                                                 <  2:2440290/93‑1 (MQ=255)

                       cTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGc                                                                 >  1:1414438/1‑93 (MQ=255)

                       cTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGc                                                                 <  1:4398734/93‑1 (MQ=255)

                        tCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCg                                                                >  2:1501250/1‑93 (MQ=255)

                         cATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGa                                                               <  2:2332946/93‑1 (MQ=255)

                         cATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGa                                                               <  1:1754678/93‑1 (MQ=255)

                         cATTGTGCGGCCTCCTTACTGTATTTCTACCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGa                                                               >  2:3170506/1‑93 (MQ=255)

                          aTTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGaa                                                              <  1:3178988/93‑1 (MQ=255)

                          aTTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGaa                                                              >  1:2130847/1‑93 (MQ=255)

                           ttGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAat                                                             <  2:3439196/93‑1 (MQ=255)

                           ttGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAat                                                             <  2:2003716/93‑1 (MQ=255)

                             gtgCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAatga                                                           <  1:901574/93‑1 (MQ=255)

                              tgCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAatgat                                                          <  2:3296266/93‑1 (MQ=255)

                              tgCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAatgat                                                          <  2:1550662/93‑1 (MQ=255)

                                cGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGAtgc                                                        <  1:4429106/93‑1 (MQ=255)

                                cGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGAtgc                                                        <  1:2653909/93‑1 (MQ=255)

                                  gCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGAtgccg                                                      >  1:2579417/1‑93 (MQ=255)

                                   cctcctTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGACAGCGAATGAtgccgt                                                     >  2:1901830/1‑93 (MQ=255)

                                    ctcctTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGAtgccgtt                                                    >  1:119735/1‑93 (MQ=255)

                                    ctcctTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGAtgccgtt                                                    <  2:2896778/93‑1 (MQ=255)

                                    ctcctTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGAtgccgtg                                                    >  2:86663/1‑92 (MQ=255)

                                    ctcctTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTAAGATCGCCCGCATGGCGATAGCGAATGAtgccgtt                                                    >  2:2671439/1‑93 (MQ=255)

                                     tcctTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGAtgccgttg                                                   >  1:3724368/1‑93 (MQ=255)

                                       ctTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGAtgccgttgcc                                                 <  1:134359/93‑1 (MQ=255)

                                        ttACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGAtgccgttgccg                                                >  2:4531460/1‑93 (MQ=255)

                                          aCTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGCTAGCGCAAGATGGCGTAGCCGTc                                              >  2:1674532/1‑93 (MQ=255)

                                           cTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCa                                             >  2:4013007/1‑93 (MQ=255)

                                            tGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCaa                                            >  2:956909/1‑93 (MQ=255)

                                            tGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCaa                                            <  1:3659104/93‑1 (MQ=255)

                                            tGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCaa                                            >  1:4039684/1‑93 (MQ=255)

                                              tATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGGTGCCGTCAATa                                          >  2:2178267/1‑93 (MQ=255)

                                               aTTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATaa                                         <  2:4632518/93‑1 (MQ=255)

                                               aTTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATaa                                         <  1:3684068/93‑1 (MQ=255)

                                                 ttCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGa                                       <  1:763728/93‑1 (MQ=255)

                                                  tctcCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGaa                                      <  2:2606338/93‑1 (MQ=255)

                                                   ctcCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGaaa                                     <  1:12717/93‑1 (MQ=255)

                                                   ctcCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGaaa                                     <  1:926298/93‑1 (MQ=255)

                                                   ctcCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGaaa                                     <  2:1851213/93‑1 (MQ=255)

                                                    tcCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAAc                                    >  1:2730879/1‑93 (MQ=255)

                                                      ccACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGt                                  >  1:4361533/1‑93 (MQ=255)

                                                      ccACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGt                                  <  1:2835847/93‑1 (MQ=255)

                                                      ccACAGCGGCTTGATCTCTTCTTCCCAGACGCGAAGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGCTGACGTCAATAAGAAACGt                                  >  2:2201907/1‑93 (MQ=255)

                                                       cacaGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGtt                                 <  2:4213059/93‑1 (MQ=255)

                                                        acaGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGttt                                <  1:2515495/93‑1 (MQ=255)

                                                         caGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTc                               <  2:4408056/93‑1 (MQ=255)

                                                          aGCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCt                              <  2:42507/93‑1 (MQ=255)

                                                           gCGGCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTg                             >  2:4297076/1‑93 (MQ=255)

                                                           gCGGCTTGATCTCTTCTTCCCAGACGCGAGGATTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTg                             <  2:4537115/93‑1 (MQ=255)

                                                             ggCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGgc                           <  2:3677867/93‑1 (MQ=255)

                                                              gCTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGgcg                          >  2:3626174/1‑93 (MQ=255)

                                                               cTTGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGgcgc                         >  1:2753520/1‑93 (MQ=255)

                                                                ttGATCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGgcgcg                        >  2:2626739/1‑93 (MQ=255)

                                                                   aTCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCa                     >  1:2151083/1‑93 (MQ=255)

                                                                   aTCTCTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCa                     <  2:3507701/93‑1 (MQ=255)

                                                                    tctcTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCAt                    >  1:1953332/1‑93 (MQ=255)

                                                                    tctcTTCTTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCAt                    <  2:3345355/93‑1 (MQ=255)

                                                                      tcttctTCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAg                  >  2:2425655/1‑93 (MQ=255)

                                                                       cttcttCCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGa                 >  2:82634/1‑93 (MQ=255)

                                                                        ttcttcCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGac                <  1:943824/93‑1 (MQ=255)

                                                                        ttcttcCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGac                <  2:622852/93‑1 (MQ=255)

                                                                         tcttcCCAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGaca               <  2:1242012/93‑1 (MQ=255)

                                                                              ccAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGACACCgag          >  1:940230/1‑93 (MQ=255)

                                                                              ccAGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGACACCgag          >  2:296398/1‑93 (MQ=255)

                                                                                aGACGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGACACCGAGAt        >  2:937854/1‑93 (MQ=255)

                                                                                  aCGCGAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGACACCGAGATcc      <  1:1213524/93‑1 (MQ=255)

                                                                                   cgcgAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGACACCGAGATCCa     <  1:2254519/93‑1 (MQ=255)

                                                                                      gAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGACACCGAGATCCAGcc  >  2:4399781/1‑93 (MQ=255)

                                                                                      gAGGGTTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGACACCGAGATCCAGcc  >  2:3077895/1‑93 (MQ=255)

GCATCAGCACGCCCAATAAAAACCTCATTGTGCGGCCTCCTTACTGTATTTCTCCCACAGCGGCTTGATCTCTTCTTCCCAGACGCGAGGATTCAGATCGCCCGCATGGCGATAGCGAATGATGCCGTTGCCGTCAATAAGAAACGTTTCTGGCGCGCCATAGACACCGAGATCCAGCC  >  NC_000913/2290403‑2290581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`