BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,309,476	C→T	A27T (GCC→ACC)	yojL ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,309,476	0	C	T	99.3%	497.9 / ‑5.6	144	A27T (GCC→ACC)	yojL	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (72/71); total (72/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 147 total aligned reads displayed.

GTTTGGCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATC  >  NC_000913/2309386‑2309565

gTTTGGCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTtggtgg                                                                                         <  1:34713/93‑1 (MQ=255)

 tttGGCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTggg                                                                                        >  1:1653020/1‑93 (MQ=255)

 tttGGCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTggg                                                                                        >  1:2450799/1‑93 (MQ=255)

  ttGGCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGt                                                                                       <  1:2542249/93‑1 (MQ=255)

    ggCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTtg                                                                                     >  1:1178029/1‑93 (MQ=255)

     gCGTCAATGCCCGGGATGCTGGCACGCCAGAATGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTtgt                                                                                    <  1:4378867/93‑1 (MQ=255)

      cGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTtgtg                                                                                   <  1:3278061/93‑1 (MQ=255)

      cGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTtgtg                                                                                   <  2:2853274/93‑1 (MQ=255)

        tCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGt                                                                                 >  1:2130925/1‑93 (MQ=255)

         cAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGtt                                                                                >  2:63450/1‑93 (MQ=255)

         cAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGtt                                                                                >  1:4654773/1‑93 (MQ=255)

           aTGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGGTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGtttt                                                                              >  2:3900502/1‑93 (MQ=255)

              cccGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGa                                                                           <  2:1417075/93‑1 (MQ=255)

              ccagggATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGa                                                                           <  1:4494105/90‑1 (MQ=255)

               ccGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGAt                                                                          >  1:1940351/1‑93 (MQ=255)

               ccGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGAt                                                                          <  2:3033776/93‑1 (MQ=255)

                cGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATc                                                                         <  1:2198380/93‑1 (MQ=255)

                   gATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGca                                                                      <  1:2380782/93‑1 (MQ=255)

                    aTGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaa                                                                     <  1:2280558/93‑1 (MQ=255)

                     tGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCCAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaac                                                                    >  1:3011299/1‑93 (MQ=255)

                      gCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaacc                                                                   >  2:1047228/1‑93 (MQ=255)

                      gCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaacc                                                                   >  1:1480326/1‑93 (MQ=255)

                       cTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaacca                                                                  <  2:3976082/93‑1 (MQ=255)

                       cTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaacca                                                                  <  2:4098602/93‑1 (MQ=255)

                        tGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaaccaa                                                                 <  2:740445/93‑1 (MQ=255)

                         ggCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaaccaac                                                                <  2:2720739/93‑1 (MQ=255)

                         ggCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaaccaac                                                                <  1:3865009/93‑1 (MQ=255)

                         ggCACGCCAGAAGCTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaaccaac                                                                <  1:4502618/93‑1 (MQ=255)

                               ccAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaa                                                          >  1:4658538/1‑93 (MQ=255)

                               ccAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaa                                                          <  2:2722274/93‑1 (MQ=255)

                               ccAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaa                                                          >  2:1226091/1‑93 (MQ=255)

                                cAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaag                                                         >  2:2220222/1‑93 (MQ=255)

                                 aGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAagaaga                                                        >  1:3953163/1‑93 (MQ=255)

                                 aGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAagaaga                                                        <  2:2843573/93‑1 (MQ=255)

                                  gAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAagag                                                       <  2:2351194/93‑1 (MQ=255)

                                  gAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAagag                                                       <  2:2387720/93‑1 (MQ=255)

                                   aaGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAgc                                                      <  2:1636222/93‑1 (MQ=255)

                                   aaGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAgc                                                      <  2:2088928/93‑1 (MQ=255)

                                   aaGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAgc                                                      <  2:2996679/93‑1 (MQ=255)

                                   aaGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAgc                                                      <  1:2919541/93‑1 (MQ=255)

                                   aaGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAgc                                                      <  2:3483158/93‑1 (MQ=255)

                                     ggTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAgcgc                                                    <  2:3684126/93‑1 (MQ=255)

                                       tACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCgg                                                  >  2:960940/1‑93 (MQ=255)

                                       tACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCgg                                                  >  1:2938167/1‑93 (MQ=255)

                                          ccATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGcag                                               >  1:1278308/1‑93 (MQ=255)

                                          ccATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGcag                                               >  1:1607499/1‑93 (MQ=255)

                                           cATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGcagc                                              >  2:3047652/1‑93 (MQ=255)

                                           cATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGcagc                                              >  2:1284270/1‑93 (MQ=255)

                                             tAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGcagcca                                            <  2:3807626/93‑1 (MQ=255)

                                             tAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGcagcca                                            <  1:3408708/93‑1 (MQ=255)

                                                ttttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCcagca                                         >  1:1487280/1‑93 (MQ=255)

                                                ttttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCcagca                                         >  2:409000/1‑93 (MQ=255)

                                                ttttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCcagca                                         <  1:3458585/93‑1 (MQ=255)

                                                ttttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCcagca                                         <  1:760955/93‑1 (MQ=255)

                                                ttttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCcagca                                         >  1:405880/1‑93 (MQ=255)

                                                 tttGCCTTCAAGAACGGTAAATTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCcagcag                                        >  2:1456918/1‑93 (MQ=255)

                                                  ttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGt                                       <  1:4381618/93‑1 (MQ=255)

                                                     ccTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGcc                                    >  2:3376328/1‑93 (MQ=255)

                                                      cTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCa                                   <  1:982877/93‑1 (MQ=255)

                                                      cTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCa                                   >  1:1240740/1‑93 (MQ=255)

                                                      cTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCa                                   <  2:3143623/93‑1 (MQ=255)

                                                       ttCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAc                                  >  1:2319992/1‑93 (MQ=255)

                                                       ttCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAc                                  <  1:2224651/93‑1 (MQ=255)

                                                       ttCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAc                                  >  2:4609993/1‑93 (MQ=255)

                                                       ttCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAc                                  >  2:3265170/1‑93 (MQ=255)

                                                       ttCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAc                                  >  1:3068776/1‑93 (MQ=255)

                                                       ttCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAc                                  >  1:3633554/1‑93 (MQ=255)

                                                         cAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAcgc                                >  1:713120/1‑93 (MQ=255)

                                                         cAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAcgc                                >  1:3191972/1‑93 (MQ=255)

                                                          aaGAACGGTAGCTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAcgcg                               <  2:2515607/93‑1 (MQ=255)

                                                           aGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCgg                              >  1:299364/1‑93 (MQ=255)

                                                           aGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCgg                              >  2:1899167/1‑93 (MQ=255)

                                                            gAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCggg                             <  2:2017110/93‑1 (MQ=255)

                                                              aCGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAAAGCGCGGCAGCCAGCAGTGCCACGCGGGTa                           >  1:2142765/1‑93 (MQ=255)

                                                                ggTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTaaa                         <  2:3665779/93‑1 (MQ=255)

                                                                ggTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTaaa                         <  2:435174/93‑1 (MQ=255)

                                                                 gTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAg                        >  2:4651390/1‑93 (MQ=255)

                                                                 gTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAg                        <  1:1318501/93‑1 (MQ=255)

                                                                  tAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGc                       <  2:829743/93‑1 (MQ=255)

                                                                  tAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGc                       >  2:846884/1‑93 (MQ=255)

                                                                  tAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGc                       <  1:4256154/93‑1 (MQ=255)

                                                                   aaCTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTCAAgat                      >  1:1860263/1‑91 (MQ=255)

                                                                   aaCTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCt                      <  2:2801011/93‑1 (MQ=255)

                                                                   aaCTTCAGGAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCt                      >  2:4568670/1‑93 (MQ=255)

                                                                    aCTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCtt                     >  2:4672092/1‑93 (MQ=255)

                                                                     cTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTa                    >  2:2907302/1‑93 (MQ=255)

                                                                     cTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTa                    <  1:2704424/93‑1 (MQ=255)

                                                                     cTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTa                    <  2:1681103/93‑1 (MQ=255)

                                                                       tCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTAtt                  <  1:1605497/93‑1 (MQ=255)

                                                                       tCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTAtt                  >  2:4237177/1‑93 (MQ=255)

                                                                         aGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTc                <  2:3952874/93‑1 (MQ=255)

                                                                         aGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTc                >  2:3753981/1‑93 (MQ=255)

                                                                          gTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTcc               <  1:1030185/93‑1 (MQ=255)

                                                                           tAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCa              <  1:2452127/93‑1 (MQ=255)

                                                                              cGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATAc           >  2:64639/1‑93 (MQ=255)

                                                                                tGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGt         <  1:1779210/93‑1 (MQ=255)

                                                                                tGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGt         >  1:998536/1‑93 (MQ=255)

                                                                                      ttGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTAt   >  2:263038/1‑93 (MQ=255)

                                                                                      ttGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGATTATTTCCATACGTGATTAt   >  1:3125553/1‑93 (MQ=255)

                                                                                       tggtggGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATc  <  1:3081954/93‑1 (MQ=255)

GTTTGGCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATC  >  NC_000913/2309386‑2309565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`