BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	254,353	C→T	G455D (GGT→GAT)	pepD ←	aminoacyl‑histidine dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	254,353	0	C	T	100.0%	644.4 / NA	178	G455D (GGT→GAT)	pepD	aminoacyl‑histidine dipeptidase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (85/93); total (85/93)

Only 100 of 183 total aligned reads displayed.

TTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGG  >  NC_000913/254263‑254442

ttCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCg                                                                                         >  1:2771595/1‑93 (MQ=255)

ttCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCg                                                                                         >  1:3605525/1‑93 (MQ=255)

 tCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCgg                                                                                        >  2:2934551/1‑93 (MQ=255)

 tCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCgg                                                                                        >  1:4073643/1‑93 (MQ=255)

    ccGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGa                                                                                     <  1:3149938/93‑1 (MQ=255)

    ccGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGa                                                                                     <  1:1582113/93‑1 (MQ=255)

      ggAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATa                                                                                   >  1:2074542/1‑93 (MQ=255)

      ggAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATa                                                                                   <  2:4125094/93‑1 (MQ=255)

       gAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATAACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAg                                                                                  <  1:3286097/93‑1 (MQ=255)

       gAATCTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAg                                                                                  <  1:2310885/93‑1 (MQ=255)

        aaTTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGt                                                                                 >  2:4647154/1‑93 (MQ=255)

        aaTTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGt                                                                                 >  1:3647745/1‑93 (MQ=255)

         aTTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGtt                                                                                <  1:1958684/93‑1 (MQ=255)

         aTTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGtt                                                                                <  1:4686104/93‑1 (MQ=255)

          tttctttcAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTg                                                                               <  2:4463374/93‑1 (MQ=255)

          tttctttcAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTg                                                                               <  1:4611211/93‑1 (MQ=255)

           ttctttcAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTgg                                                                              <  2:1042264/93‑1 (MQ=255)

            tctttcAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGc                                                                             >  1:64442/1‑93 (MQ=255)

             ctttcAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGcc                                                                            <  2:1134124/93‑1 (MQ=255)

              tttcAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGCGTGGATCGGTGATAGTTTGccc                                                                           >  2:2810650/1‑93 (MQ=255)

                 cagcagTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGAt                                                                        <  1:2871974/93‑1 (MQ=255)

                  agcagTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTTGCCCGATa                                                                       >  2:3371817/1‑93 (MQ=255)

                  agcagTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATa                                                                       <  2:4344954/93‑1 (MQ=255)

                   gcagTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAg                                                                      >  2:3614072/1‑93 (MQ=255)

                    cagTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGa                                                                     <  2:2827197/93‑1 (MQ=255)

                    cagTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGa                                                                     <  1:871792/93‑1 (MQ=255)

                    cagTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGa                                                                     <  1:2121384/93‑1 (MQ=255)

                      gTTCAGTCGGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGaaa                                                                   <  2:868407/93‑1 (MQ=255)

                      gTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCACGATAGaaa                                                                   >  2:3779639/1‑93 (MQ=255)

                       ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAAc                                                                  <  2:3001580/93‑1 (MQ=255)

                       ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAAc                                                                  <  1:2711368/93‑1 (MQ=255)

                       ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAAc                                                                  <  1:2284840/93‑1 (MQ=255)

                       ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAAc                                                                  <  2:3942232/93‑1 (MQ=255)

                       ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAAc                                                                  <  1:2035072/93‑1 (MQ=255)

                       ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAAc                                                                  <  2:4354605/93‑1 (MQ=255)

                        tcagtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAAcc                                                                 <  1:4478169/93‑1 (MQ=255)

                          agtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACAAt                                                               <  1:1146911/93‑1 (MQ=255)

                           gtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATg                                                              >  2:2104362/1‑93 (MQ=255)

                           gtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATg                                                              <  1:4313155/93‑1 (MQ=255)

                             cagcagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTc                                                            >  2:1851620/1‑93 (MQ=255)

                              agcagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTcc                                                           >  2:3095146/1‑93 (MQ=255)

                               gcagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCa                                                          <  1:2215670/93‑1 (MQ=255)

                               gcagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCa                                                          >  1:844383/1‑93 (MQ=255)

                                  gtgtCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCAttt                                                       >  2:897254/1‑93 (MQ=255)

                                   tgtCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTc                                                      <  2:2353407/93‑1 (MQ=255)

                                   tgtCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTc                                                      >  2:908207/1‑93 (MQ=255)

                                    ttCCAGTAATGACCTACGCTTTCGATGTTAACTTTCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTcc                                                     <  1:1043878/92‑1 (MQ=255)

                                    gtCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTcc                                                     >  1:986765/1‑93 (MQ=255)

                                       cAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGa                                                  <  2:4201889/93‑1 (MQ=255)

                                       cAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGa                                                  <  1:3334435/93‑1 (MQ=255)

                                        aGTAATGACCTACGCTGTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGAt                                                 <  1:3039504/93‑1 (MQ=255)

                                         gTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATa                                                >  1:896369/1‑93 (MQ=255)

                                          tAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATAc                                               <  2:2960015/93‑1 (MQ=255)

                                            aTGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACgg                                             >  1:9222/1‑93 (MQ=255)

                                             tGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGt                                            <  2:1704981/93‑1 (MQ=255)

                                              gACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGtt                                           >  1:2080013/1‑93 (MQ=255)

                                               aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGttt                                          >  1:2480767/1‑93 (MQ=255)

                                               aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGttt                                          <  1:427585/93‑1 (MQ=255)

                                               aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGttt                                          >  2:2652863/1‑93 (MQ=255)

                                                ccTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGtttt                                         >  1:1503722/1‑93 (MQ=255)

                                                 cTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGttttt                                        >  1:4005701/1‑93 (MQ=255)

                                                  tACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGtttttt                                       >  2:3729347/1‑93 (MQ=255)

                                                  tACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGtttttt                                       >  2:2095327/1‑93 (MQ=255)

                                                     gCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGaa                                    <  1:1116870/93‑1 (MQ=255)

                                                      cTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTGGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAAc                                   <  2:1823111/93‑1 (MQ=255)

                                                       tttCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACa                                  >  2:4386397/1‑93 (MQ=255)

                                                       tttCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACa                                  <  1:1623996/93‑1 (MQ=255)

                                                       tttCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACa                                  >  2:1203206/1‑93 (MQ=255)

                                                         tCGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGa                                <  1:2563789/93‑1 (MQ=255)

                                                          cGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGAc                               <  2:504373/93‑1 (MQ=255)

                                                          cGATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGAc                               <  2:1651665/93‑1 (MQ=255)

                                                           gATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGAcc                              <  2:517591/93‑1 (MQ=255)

                                                           gATGTGAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGAcc                              >  2:1510831/1‑93 (MQ=255)

                                                             tgtgAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACcac                            <  2:3750496/93‑1 (MQ=255)

                                                              gtgAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACcaca                           <  1:4567758/93‑1 (MQ=255)

                                                               tgAACTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACAt                          <  1:2197120/93‑1 (MQ=255)

                                                                  aCTTGCTTATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTcc                       <  2:1136571/93‑1 (MQ=255)

                                                                   cTTGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCa                      <  1:355358/93‑1 (MQ=255)

                                                                    ttGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAg                     <  2:4332257/93‑1 (MQ=255)

                                                                    ttGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAg                     >  1:2761968/1‑93 (MQ=255)

                                                                    ttGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAAACCACATTCAAg                     >  2:3393644/1‑93 (MQ=255)

                                                                     tGCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAgg                    >  1:4654677/1‑93 (MQ=255)

                                                                      gCTCATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGc                   <  1:3949525/93‑1 (MQ=255)

                                                                         cATCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCcg                <  2:2230851/93‑1 (MQ=255)

                                                                           tCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCcgcg              >  1:4131559/1‑93 (MQ=255)

                                                                           tCCGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCcgcg              >  1:1805061/1‑93 (MQ=255)

                                                                            ccGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGt             <  2:2548228/93‑1 (MQ=255)

                                                                             cGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTg            <  2:740573/93‑1 (MQ=255)

                                                                             cGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTg            <  2:641791/93‑1 (MQ=255)

                                                                             cGGAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTg            <  2:138596/93‑1 (MQ=255)

                                                                              ggAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTgg           >  1:1237415/1‑93 (MQ=255)

                                                                              ggAGAGTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTgg           >  1:1842113/1‑93 (MQ=255)

                                                                                 gagTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATa        >  2:2719426/1‑93 (MQ=255)

                                                                                 gagTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATa        >  2:1527736/1‑93 (MQ=255)

                                                                                 gagTGTGGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGGTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATa        >  2:2687285/1‑93 (MQ=255)

                                                                                    tgtgGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATc     <  1:2973339/93‑1 (MQ=255)

                                                                                      tgGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTg   <  1:2176089/93‑1 (MQ=255)

                                                                                      tgGATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTg   <  2:1918630/93‑1 (MQ=255)

                                                                                       ggATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTgg  >  1:28185/1‑93 (MQ=255)

                                                                                       ggATCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTgg  >  2:1215733/1‑93 (MQ=255)

TTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGG  >  NC_000913/254263‑254442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`