BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,349,841	T→C	D185G (GAC→GGC)	glpT ←	sn‑glycerol‑3‑phosphate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,349,841	0	T	C	100.0%	375.4 / NA	118	D185G (GAC→GGC)	glpT	sn‑glycerol‑3‑phosphate permease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (64/54); total (64/54)

Only 100 of 127 total aligned reads displayed.

CAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCC  >  NC_000913/2349751‑2349929

  ggATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCAtt                                                                                      >  1:1008834/1‑93 (MQ=255)

  ggATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCAtt                                                                                      <  1:1658655/93‑1 (MQ=255)

   gATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTg                                                                                     >  1:182226/1‑93 (MQ=255)

   gATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTg                                                                                     >  1:3756300/1‑93 (MQ=255)

    aTTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGa                                                                                    <  1:4527111/93‑1 (MQ=255)

     ttGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGaa                                                                                   <  1:360517/93‑1 (MQ=255)

     ttGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGaa                                                                                   <  1:2277634/93‑1 (MQ=255)

     ttGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGaa                                                                                   <  1:2975410/93‑1 (MQ=255)

       tcGGGCTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAAcc                                                                                 <  2:2278234/92‑1 (MQ=255)

       gCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAAcc                                                                                 <  1:3642701/93‑1 (MQ=255)

       gCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAAcc                                                                                 <  1:2668386/93‑1 (MQ=255)

        cGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCa                                                                                <  1:3216970/93‑1 (MQ=255)

         ggggTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAg                                                                               <  1:1149953/93‑1 (MQ=255)

         ggggTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAg                                                                               >  1:2596116/1‑93 (MQ=255)

         ggggTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAg                                                                               <  2:896016/93‑1 (MQ=255)

          gggTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAgg                                                                              >  2:3030832/1‑93 (MQ=255)

           ggTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGc                                                                             <  2:3936322/93‑1 (MQ=255)

            gTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGTGCAGAAAGCAGGCATATCGAGCGCCGCATGCCAGCCATTGAAGCAGGcc                                                                            >  1:146164/1‑93 (MQ=255)

            gTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGcc                                                                            <  2:2589882/93‑1 (MQ=255)

            gTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGcc                                                                            <  2:3015519/93‑1 (MQ=255)

            gTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGcc                                                                            <  1:1021991/93‑1 (MQ=255)

              aTCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCAt                                                                          <  2:2744509/93‑1 (MQ=255)

               tCGCGCATCATCGCAAAGTGGAATAATGCATCTAGAATGGGGAATAAAGTGTTCATATAGAGCGCCGCATGCCAGCCATTGAACCAGTCCATc                                                                         <  2:4458425/93‑1 (MQ=255)

               tCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATc                                                                         <  1:2960279/93‑1 (MQ=255)

                   gcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGCACCAGGCCATCCCca                                                                     >  1:3863191/1‑93 (MQ=255)

                    cagcatcGCAAAGGTGAATAATGCCACCAGAATGCCGCAGAAAGCAGGCATGTAGAGCCCCGAATGCAAGCCAGTGAACCAGGCCCTCCCcag                                                                    <  2:4374514/90‑1 (MQ=255)

                     atcatcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCcagc                                                                   >  1:1563725/1‑93 (MQ=255)

                      tcatcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCcagca                                                                  >  2:2501048/1‑93 (MQ=255)

                        atcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAgg                                                                >  1:3339549/1‑93 (MQ=255)

                           gCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAAc                                                             <  1:617470/93‑1 (MQ=255)

                           gCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAAc                                                             >  2:3268433/1‑93 (MQ=255)

                            cAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAAca                                                            >  1:944054/1‑93 (MQ=255)

                            cAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAAca                                                            >  2:3262902/1‑93 (MQ=255)

                            cAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGACATCCCCAGAAGGAAca                                                            >  2:1226247/1‑93 (MQ=255)

                             aaaGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAAcag                                                           >  2:40876/1‑93 (MQ=255)

                              aaGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAAcagc                                                          >  1:3852502/1‑93 (MQ=255)

                              aaGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAAcagc                                                          <  2:4460298/93‑1 (MQ=255)

                               aGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCCGGCCATCCCCAGCAGGAAcagca                                                         >  1:96609/1‑93 (MQ=255)

                               aGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAAcagca                                                         >  2:1570167/1‑93 (MQ=255)

                                ggCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGAAAcagcag                                                        >  1:2521259/1‑93 (MQ=255)

                                  cGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAgcg                                                      <  1:2764279/93‑1 (MQ=255)

                                   gAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAgcgg                                                     <  1:4056133/93‑1 (MQ=255)

                                   gAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAgcgg                                                     <  1:1787622/93‑1 (MQ=255)

                                    aataatGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAgcggc                                                    >  1:2444334/1‑93 (MQ=255)

                                    aataatGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAgcggc                                                    <  1:2755096/93‑1 (MQ=255)

                                       aatGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGa                                                 >  2:1491052/1‑93 (MQ=255)

                                        atGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGaa                                                <  1:1935517/93‑1 (MQ=255)

                                          gCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATa                                              <  1:3985288/93‑1 (MQ=255)

                                           ccaccaGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATac                                             <  2:2994365/93‑1 (MQ=255)

                                            caccaGAATGGCGCAGAAAGCATGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATacc                                            >  1:3488860/1‑93 (MQ=255)

                                            caccaGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATacc                                            >  2:744792/1‑93 (MQ=255)

                                             accaGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATacca                                           >  2:186462/1‑93 (MQ=255)

                                              ccaGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATaccac                                          >  2:2212112/1‑93 (MQ=255)

                                               caGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATaccacc                                         >  2:3286123/1‑93 (MQ=255)

                                                aGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATaccacca                                        >  2:4458682/1‑93 (MQ=255)

                                                 gAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATaccaccac                                       <  1:2372447/93‑1 (MQ=255)

                                                  aaTGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATaccaccacc                                      >  1:2138936/1‑93 (MQ=255)

                                                    tGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGa                                    >  1:3881160/1‑93 (MQ=255)

                                                          aGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTtgt                              <  2:3498615/93‑1 (MQ=255)

                                                          aGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTtgt                              <  2:910409/93‑1 (MQ=255)

                                                          aGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTtgt                              <  1:495249/93‑1 (MQ=255)

                                                          aGAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTtga                              >  1:1055774/1‑92 (MQ=255)

                                                           gAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTtgtg                             >  2:1532475/1‑93 (MQ=255)

                                                           gAAAGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTtgtg                             >  2:1411634/1‑93 (MQ=255)

                                                             aaGCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTgcg                           >  2:2970721/1‑93 (MQ=255)

                                                               gCAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGca                         <  1:2129505/93‑1 (MQ=255)

                                                                cAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGcac                        >  2:3563631/1‑93 (MQ=255)

                                                                cAGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGcac                        >  2:650387/1‑93 (MQ=255)

                                                                 aGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGcaca                       >  2:934528/1‑93 (MQ=255)

                                                                 aGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGcaca                       <  2:2221305/93‑1 (MQ=255)

                                                                 aGGCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGCTGTGCGcaca                       >  2:4167077/1‑93 (MQ=255)

                                                                   gCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATAGCACCACCGACGTTGTGCGCACAGt                     >  2:3909263/1‑93 (MQ=255)

                                                                   gCATATAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGt                     <  2:1416123/93‑1 (MQ=255)

                                                                    cATATAGAGCGCCACATCCCAGCCACTGAACCAGGCCAACCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGtt                    <  1:4530514/93‑1 (MQ=255)

                                                                     atatAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTc                   >  1:3767741/1‑93 (MQ=255)

                                                                     atatAGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTc                   >  2:3521448/1‑93 (MQ=255)

                                                                      tataGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTcc                  >  2:1752935/1‑93 (MQ=255)

                                                                      tataGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTcc                  <  2:1448554/93‑1 (MQ=255)

                                                                      tataGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTcc                  >  1:1256878/1‑93 (MQ=255)

                                                                      tataGAGCGCCGCATGCCAGCCATTCAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTcc                  <  2:3998448/93‑1 (MQ=255)

                                                                        taGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACGACCACCGACGTTGTGCGCACAGTTCcac                >  2:3422832/1‑93 (MQ=255)

                                                                        taGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCcac                <  2:171172/93‑1 (MQ=255)

                                                                        taGAGCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCcac                <  1:4301225/93‑1 (MQ=255)

                                                                         agagCTCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACATTGTGCGCACAGTGCcaca               <  2:4542641/93‑1 (MQ=255)

                                                                         agagCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCcaca               <  1:4426574/93‑1 (MQ=255)

                                                                          gagCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATGCCACCACCGACGTTGTGCGCACAGTTCCacac              <  1:489626/93‑1 (MQ=255)

                                                                          gagCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCacac              <  1:933535/93‑1 (MQ=255)

                                                                          gagCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCacac              <  2:75286/93‑1 (MQ=255)

                                                                          gagCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCacac              <  2:2596116/93‑1 (MQ=255)

                                                                           agCGCCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACt             <  1:1932279/93‑1 (MQ=255)

                                                                            gcgcCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTg            >  1:3392458/1‑93 (MQ=255)

                                                                            gcgcCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTg            >  2:4436050/1‑93 (MQ=255)

                                                                            gcgcCGCATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTg            >  1:1819022/1‑93 (MQ=255)

                                                                               ccgcATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGaca         >  1:1748759/1‑93 (MQ=255)

                                                                                 gcATGCCAGCCATTGAACCAGGCCATCTCCAGCAGGGACAGCAGCGGCGGAATACCACCACCGACGCTGCGCGCACAGTTCCACGCTGAcaca       >  2:669239/1‑93 (MQ=255)

                                                                                  cATGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACaa      <  2:2053838/93‑1 (MQ=255)

                                                                                   aTGCCAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCGCCACCGACGTTGTGCGCACAGTTCCACACTGACACAAt     <  1:2054212/93‑1 (MQ=255)

                                                                                    tGCCAGCCCTTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCCCAGTTCCACACTGACACAATg    <  1:21415/93‑1 (MQ=255)

                                                                                     gccagccaTTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATgc   <  1:613916/93‑1 (MQ=255)

                                                                                      ccAGCCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATgcc  >  2:2274021/1‑93 (MQ=255)

CAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCC  >  NC_000913/2349751‑2349929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`