BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,375,871	T→C	N470S (AAC→AGC)	menD ←	2‑oxoglutarate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,375,871	0	T	C	100.0%	462.5 / NA	150	N470S (AAC→AGC)	menD	2‑oxoglutarate decarboxylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (71/79); total (71/79)

Only 100 of 168 total aligned reads displayed.

GCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCC  >  NC_000913/2375781‑2375960

gcgTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctg                                                                                         >  1:2901256/1‑93 (MQ=255)

   tGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttg                                                                                      >  1:2798437/1‑93 (MQ=255)

   tGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttg                                                                                      >  1:1990094/1‑93 (MQ=255)

   tGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGACgctgttg                                                                                      >  1:3465462/1‑93 (MQ=255)

    gCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttgt                                                                                     <  2:2177741/93‑1 (MQ=255)

    gCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttgt                                                                                     >  1:3871277/1‑93 (MQ=255)

      tCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTc                                                                                   >  1:3716195/1‑93 (MQ=255)

      tCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTc                                                                                   <  1:3964505/93‑1 (MQ=255)

       cAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTca                                                                                  >  2:2763733/1‑93 (MQ=255)

        aaaaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcac                                                                                 >  2:2166702/1‑93 (MQ=255)

        aaaaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcac                                                                                 >  2:320993/1‑93 (MQ=255)

         aaaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGTGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcacc                                                                                >  2:1715040/1‑93 (MQ=255)

         aaaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcacc                                                                                <  2:454688/93‑1 (MQ=255)

             ggACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACaa                                                                            >  2:303207/1‑93 (MQ=255)

             ggACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACaa                                                                            >  2:871293/1‑93 (MQ=255)

              gACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAAt                                                                           >  2:1933251/1‑93 (MQ=255)

              gACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAAt                                                                           >  2:4447685/1‑93 (MQ=255)

               aCGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAAtt                                                                          <  1:1306442/93‑1 (MQ=255)

                 gTTTTGCGGCATCAGATAGTAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTaa                                                                        >  2:4579307/1‑93 (MQ=255)

                 gTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTaa                                                                        >  1:2023799/1‑93 (MQ=255)

                 gTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTaa                                                                        >  2:3766794/1‑93 (MQ=255)

                  ttttGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAAt                                                                       <  1:285038/93‑1 (MQ=255)

                      gCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCa                                                                   <  2:2336721/93‑1 (MQ=255)

                       cGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAg                                                                  <  1:1732145/93‑1 (MQ=255)

                        ggCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgc                                                                 <  1:3548237/93‑1 (MQ=255)

                         gCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgcg                                                                >  2:1381503/1‑93 (MQ=255)

                           aTCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgcggc                                                              <  1:1073882/93‑1 (MQ=255)

                            tCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgcggcg                                                             >  2:2647219/1‑93 (MQ=255)

                             cAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGgcgc                                                            <  1:113645/93‑1 (MQ=255)

                             cAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGgcgc                                                            >  2:554126/1‑93 (MQ=255)

                                 tAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCCGaa                                                        >  1:2254123/1‑93 (MQ=255)

                                 tAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGaa                                                        >  2:2219375/1‑93 (MQ=255)

                                 tAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGaa                                                        >  1:830333/1‑93 (MQ=255)

                                 tAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGaa                                                        >  1:38139/1‑93 (MQ=255)

                                  aGAAACGCTCAGGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGaaa                                                       <  1:191363/93‑1 (MQ=255)

                                  aGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGaaa                                                       <  2:4665825/93‑1 (MQ=255)

                                   gAAACGCTCACGCGCGCTTGGCGGCGTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAc                                                      <  2:3946378/93‑1 (MQ=255)

                                    aaccgctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAcc                                                     <  1:2583872/90‑1 (MQ=255)

                                    aaaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAcc                                                     >  1:3119972/1‑93 (MQ=255)

                                    aaaCGCTCACGCTCGCTTTGCGGCGTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAcc                                                     <  1:3795032/93‑1 (MQ=255)

                                     aaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAACt                                                    >  2:2656903/1‑93 (MQ=255)

                                      acgctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTg                                                   <  2:3464396/93‑1 (MQ=255)

                                        gctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGAc                                                 <  1:753309/93‑1 (MQ=255)

                                         ctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACg                                                >  1:145774/1‑93 (MQ=255)

                                           cacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCa                                              <  2:4318063/93‑1 (MQ=255)

                                            ccgctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCaa                                             <  2:3149070/92‑1 (MQ=255)

                                             cgctcgctTGGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAAt                                            <  2:4137619/93‑1 (MQ=255)

                                              gctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATa                                           >  1:3623985/1‑93 (MQ=255)

                                               ctcgttTTGCGGCGTTGGCAACGGCGAGAAAATTTCCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATaa                                          <  2:4447452/93‑1 (MQ=255)

                                               ctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATaa                                          <  1:669907/93‑1 (MQ=255)

                                               ctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATaa                                          >  1:1439649/1‑93 (MQ=255)

                                               ctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATaa                                          >  2:2967103/1‑93 (MQ=255)

                                               ctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATaa                                          >  2:2151364/1‑93 (MQ=255)

                                                tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAc                                         >  1:4117377/1‑93 (MQ=255)

                                                tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAc                                         >  1:3846024/1‑93 (MQ=255)

                                                   ctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGcc                                      <  2:3898764/93‑1 (MQ=255)

                                                   ctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGcc                                      <  1:1549372/93‑1 (MQ=255)

                                                   ctTTGCGGCGTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGcc                                      <  2:2741695/93‑1 (MQ=255)

                                                     ttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAg                                    <  2:2860472/93‑1 (MQ=255)

                                                     ttGCGGCGTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAg                                    <  1:3694918/93‑1 (MQ=255)

                                                       gcggcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgcg                                  >  1:2351209/1‑93 (MQ=255)

                                                          gcgTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGt                               <  2:1135392/93‑1 (MQ=255)

                                                           cgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                              >  2:1733340/1‑93 (MQ=255)

                                                           cgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                              >  1:790327/1‑93 (MQ=255)

                                                           cgTTGACAACAGCGAGAAAATTTCCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                              <  1:3208323/93‑1 (MQ=255)

                                                            gtgggCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTg                             <  1:1931876/90‑1 (MQ=255)

                                                            gTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTg                             >  2:269272/1‑93 (MQ=255)

                                                             ttGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga                            <  1:3321344/93‑1 (MQ=255)

                                                             ttGGCAACACCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga                            <  1:4225442/93‑1 (MQ=255)

                                                             ttGGCAACACCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga                            <  2:4483373/93‑1 (MQ=255)

                                                                gaaaCAGCGATAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAt                         <  2:202159/91‑1 (MQ=255)

                                                                gCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAt                         >  2:3364043/1‑93 (MQ=255)

                                                                  aaCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGCAACCTGACGCAATAACGCCAGCCCATTGAGATCg                       >  1:3330766/1‑93 (MQ=255)

                                                                   aaaGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                      <  2:2819722/91‑1 (MQ=255)

                                                                   aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                      >  1:724732/1‑93 (MQ=255)

                                                                   aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                      >  1:3926590/1‑93 (MQ=255)

                                                                     aGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaa                    <  1:1586295/93‑1 (MQ=255)

                                                                     aGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaa                    <  2:2147112/93‑1 (MQ=255)

                                                                     aGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaa                    >  2:4216047/1‑93 (MQ=255)

                                                                      gCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaaa                   <  1:2297670/93‑1 (MQ=255)

                                                                      gCGAGAAAAGTTGCCCGCCGCTGTTGTTCACCACAATTAATCCCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaaa                   <  1:982360/93‑1 (MQ=255)

                                                                       cGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAg                  <  2:3266848/93‑1 (MQ=255)

                                                                       cGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAg                  >  2:1960003/1‑93 (MQ=255)

                                                                       cGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAg                  <  2:1154951/93‑1 (MQ=255)

                                                                        aagaAAATTGCCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGt                 <  2:146939/92‑1 (MQ=255)

                                                                          gaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGc               >  2:1683461/1‑93 (MQ=255)

                                                                          gaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGc               >  1:1980530/1‑93 (MQ=255)

                                                                            aaaTTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCgg             <  2:954498/93‑1 (MQ=255)

                                                                             aaTTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGga            >  2:3557236/1‑93 (MQ=255)

                                                                              aTTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgag           >  1:2431990/1‑93 (MQ=255)

                                                                              aTTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgag           <  1:2249687/93‑1 (MQ=255)

                                                                              aTTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgag           >  1:1914352/1‑93 (MQ=255)

                                                                               tttGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgaga          <  2:4437316/93‑1 (MQ=255)

                                                                               tttGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgaga          <  1:3566042/93‑1 (MQ=255)

                                                                               tttGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgaga          <  1:103438/93‑1 (MQ=255)

                                                                                tggCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagag         <  2:1030931/91‑1 (MQ=255)

                                                                                 tGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagaga        <  1:3111824/93‑1 (MQ=255)

                                                                                  ccccGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAt       <  2:771431/92‑1 (MQ=255)

                                                                                    ccgccgCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCg     >  1:3681056/1‑93 (MQ=255)

                                                                                    ccgccgCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCAGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATcc     >  1:3041566/1‑92 (MQ=255)

GCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCC  >  NC_000913/2375781‑2375960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`