BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	271,281	G→A	G76G (GGG→GGA)	b0257 →	putative transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	271,281	0	G	A	99.5%	667.0 / ‑5.5	188	G76G (GGG→GGA)	b0257	putative transposase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (96/91); total (97/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

Only 100 of 201 total aligned reads displayed.

TGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTCGCCGT  >  NC_000913/271191‑271373

tGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACt                                                                                            >  1:2050815/1‑93 (MQ=255)

    ggAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTc                                                                                        >  2:1991819/1‑93 (MQ=255)

     gAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCa                                                                                       >  1:1345456/1‑93 (MQ=255)

       gCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGt                                                                                     >  2:1273896/1‑93 (MQ=255)

       gCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGt                                                                                     <  1:1011332/93‑1 (MQ=255)

       gCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGt                                                                                     <  1:615408/93‑1 (MQ=255)

       gCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGt                                                                                     >  1:514523/1‑93 (MQ=255)

        cAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGtt                                                                                    <  1:4102567/93‑1 (MQ=255)

        cAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGtt                                                                                    >  1:1910538/1‑93 (MQ=255)

         aagaagCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTg                                                                                   >  2:3580472/1‑93 (MQ=255)

         aagaagCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTg                                                                                   >  2:3818129/1‑93 (MQ=255)

          agaagCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGc                                                                                  <  2:2981172/93‑1 (MQ=255)

          agaagCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGc                                                                                  >  2:4449291/1‑93 (MQ=255)

             agCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCcag                                                                               >  2:4583717/1‑93 (MQ=255)

             agCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCcag                                                                               <  1:770120/93‑1 (MQ=255)

             agCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCcag                                                                               >  1:2461941/1‑93 (MQ=255)

                 tCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCagcagc                                                                           <  2:3845441/93‑1 (MQ=255)

                     cacaATGGCAACCCAGAGAGGATACAAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTGCGTTGCCCGCAGCCGAc                                                                       >  2:4333647/1‑93 (MQ=17)

                      acaATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACt                                                                      >  1:4511803/1‑93 (MQ=255)

                        aaTGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCa                                                                    >  1:1674048/1‑93 (MQ=255)

                          aggCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCCTGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCAcc                                                                  <  1:3293479/92‑1 (MQ=255)

                           ggCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCg                                                                 >  1:3008296/1‑93 (MQ=255)

                            gCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCgg                                                                <  1:469723/93‑1 (MQ=255)

                            gCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCgg                                                                <  2:655685/93‑1 (MQ=255)

                             cAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGt                                                               <  1:1471029/93‑1 (MQ=255)

                              aaCCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGta                                                              <  2:3130459/93‑1 (MQ=255)

                               aCCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGtat                                                             <  1:3142990/93‑1 (MQ=255)

                                cccAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGtata                                                            >  2:2085513/1‑93 (MQ=255)

                                cccAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGtata                                                            <  1:916688/93‑1 (MQ=255)

                                 ccAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGGGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATaa                                                           >  1:801897/1‑93 (MQ=255)

                                 ccAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATaa                                                           <  1:1178925/93‑1 (MQ=255)

                                 ccAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATaa                                                           <  1:2081768/93‑1 (MQ=255)

                                 ccAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATaa                                                           >  1:1727284/1‑93 (MQ=255)

                                 ccAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATaa                                                           >  2:1462590/1‑93 (MQ=255)

                                  cAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAg                                                          <  2:491508/93‑1 (MQ=255)

                                   agagagGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGc                                                         >  2:4527995/1‑93 (MQ=255)

                                   agagagGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGc                                                         <  1:1287835/93‑1 (MQ=255)

                                    gagagGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGCATGGTCAGTTGCCAGCAGCCCACTCCCCGGTATAAGCg                                                        <  2:319139/93‑1 (MQ=17)

                                    gagagGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCg                                                        >  1:2875499/1‑93 (MQ=255)

                                    gagagGATACCAGATGGGGCACTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCg                                                        >  2:1825666/1‑93 (MQ=255)

                                     agagGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCgg                                                       >  1:1559547/1‑92 (MQ=255)

                                      gagGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTg                                                      >  2:3713102/1‑93 (MQ=255)

                                      gagGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTg                                                      >  1:297702/1‑93 (MQ=255)

                                        ggATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGc                                                    <  2:1145156/93‑1 (MQ=255)

                                        ggATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTCGTACTCGTCAGCTGCCAGCAGCCGACTCACCGGTATAAACGTgac                                                    >  2:1003237/1‑91 (MQ=11)

                                         gATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCg                                                   >  1:4300942/1‑93 (MQ=255)

                                          aTACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCgg                                                  >  2:1633532/1‑93 (MQ=255)

                                          aTACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCgg                                                  >  2:1915291/1‑93 (MQ=255)

                                           tACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGGTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCggg                                                 >  1:349248/1‑92 (MQ=255)

                                               aGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGTACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATg                                             <  2:4202137/93‑1 (MQ=255)

                                               aGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATg                                             >  1:1151191/1‑93 (MQ=255)

                                                 aTGGGGCGCTGGGTTACTGGCAGAGGTATGAAAGAGCTGGGTCTGGTCAGTCGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAg                                           <  1:2556618/93‑1 (MQ=17)

                                                 aTGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAg                                           >  2:3972542/1‑93 (MQ=255)

                                                 aTGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAg                                           <  2:3248194/93‑1 (MQ=255)

                                                  tGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGc                                          <  1:1588450/93‑1 (MQ=255)

                                                  tGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGc                                          >  2:2342523/1‑93 (MQ=255)

                                                   ggggCGCTGGTTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCa                                         <  1:182132/93‑1 (MQ=255)

                                                   ggggCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCa                                         <  2:3599584/93‑1 (MQ=255)

                                                      gcgcTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCATTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGt                                      <  2:807412/93‑1 (MQ=255)

                                                         cTGGCTTGCTGGCAGCCTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGc                                   <  1:1407306/93‑1 (MQ=255)

                                                         cTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGc                                   <  2:2996413/93‑1 (MQ=255)

                                                          tGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCt                                  >  1:1836684/1‑93 (MQ=255)

                                                           ggCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTa                                 <  1:463222/93‑1 (MQ=255)

                                                           ggCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTa                                 >  2:2745355/1‑93 (MQ=255)

                                                           ggCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTa                                 >  2:2369732/1‑93 (MQ=255)

                                                            gCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTAt                                >  1:2862121/1‑93 (MQ=255)

                                                             cTTGCTGGCAGACTCATGAAAGAGCTGGGACTTGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATc                               <  2:3037353/93‑1 (MQ=255)

                                                              ttGCTGGCAGCCTCATGAAAGAGCTGGGACTGGTCAGTGGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATcc                              <  2:2194658/93‑1 (MQ=255)

                                                              ttGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATcc                              <  2:556592/93‑1 (MQ=255)

                                                              ttGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATcc                              >  2:1722308/1‑93 (MQ=255)

                                                              ttGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATcc                              <  2:1222488/93‑1 (MQ=255)

                                                               tGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATccc                             >  1:4149909/1‑93 (MQ=255)

                                                                   ggCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAat                         <  2:3382374/93‑1 (MQ=255)

                                                                   ggCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAat                         <  2:3639905/93‑1 (MQ=255)

                                                                    gCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAatc                        <  1:1953811/93‑1 (MQ=255)

                                                                    gCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAatc                        >  2:4399918/1‑93 (MQ=255)

                                                                      aGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAatcat                      >  2:2303163/1‑93 (MQ=255)

                                                                       gACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAatcatc                     >  1:709308/1‑93 (MQ=255)

                                                                       gACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAatcatc                     <  2:2574014/93‑1 (MQ=255)

                                                                       gACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAatcatc                     >  1:2201399/1‑93 (MQ=255)

                                                                       gACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAatcatc                     >  1:2698052/1‑93 (MQ=255)

                                                                        aCTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCt                    <  1:2105921/93‑1 (MQ=255)

                                                                         cTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCtt                   <  1:3420369/93‑1 (MQ=255)

                                                                         cTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCtt                   <  1:3627540/93‑1 (MQ=255)

                                                                          tCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTg                  >  2:1340683/1‑93 (MQ=255)

                                                                           cATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGa                 <  1:2240789/93‑1 (MQ=255)

                                                                            aTGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAg                >  1:1089750/1‑93 (MQ=255)

                                                                             tGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGc               >  1:2379925/1‑93 (MQ=255)

                                                                             tGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGc               >  2:1113857/1‑93 (MQ=255)

                                                                               aaaGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGa             >  1:1199243/1‑93 (MQ=255)

                                                                                 agagCTGTGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACa           <  1:287871/93‑1 (MQ=255)

                                                                                 agagCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACa           <  1:2620233/93‑1 (MQ=255)

                                                                                 agagCGGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACa           <  2:2512933/93‑1 (MQ=255)

                                                                                  gagCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAg          <  1:417755/93‑1 (MQ=255)

                                                                                  gagCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAg          >  2:1547431/1‑93 (MQ=255)

                                                                                    gCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGtt        >  1:3866015/1‑93 (MQ=255)

                                                                                    gCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGtt        >  1:2943032/1‑93 (MQ=255)

                                                                                    gCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGtt        <  1:1995512/93‑1 (MQ=255)

                                                                                     cTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTc       <  1:3814458/93‑1 (MQ=255)

                                                                                       gggaCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTCGc     <  2:2616709/93‑1 (MQ=255)

TGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTCGCCGT  >  NC_000913/271191‑271373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`