Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,465,657 T→C Y365H (TAT→CAT)  intC → putative prophage Sf6‑like integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,465,6570TC99.4% 528.3 / ‑4.9 167Y365H (TAT→CAT) intCputative prophage Sf6‑like integrase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (92/74);  total (93/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01

Only 100 of 172 total aligned reads displayed.

ACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGTATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATA  >  NC_000913/2465568‑2465745
                                                                                         |                                                                                        
aCGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCAtc                                                                                       >  2:491534/1‑93 (MQ=255)
aCGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCAtc                                                                                       <  1:375145/93‑1 (MQ=255)
  gAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCAtctc                                                                                     <  1:399505/93‑1 (MQ=255)
   aGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCg                                                                                    >  1:3873666/1‑93 (MQ=255)
    gCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGa                                                                                   <  1:3016725/93‑1 (MQ=255)
    gCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGa                                                                                   <  2:388823/93‑1 (MQ=255)
    gCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGa                                                                                   >  2:2489584/1‑93 (MQ=255)
      aCGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATa                                                                                 <  1:3508205/93‑1 (MQ=255)
       cGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATaa                                                                                >  1:4186034/1‑93 (MQ=255)
       cGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATaa                                                                                >  2:796092/1‑93 (MQ=255)
       cGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATaa                                                                                >  2:543975/1‑93 (MQ=255)
        gAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATaaa                                                                               <  2:3193602/93‑1 (MQ=255)
        gAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATaaa                                                                               <  2:1774337/93‑1 (MQ=255)
        gAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATaaa                                                                               <  2:116368/93‑1 (MQ=255)
         aaTGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGGTTTACAACCATGCTCAGCATCTCGATAAAc                                                                              >  2:3338064/1‑93 (MQ=255)
         aaTGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAAc                                                                              <  1:1672252/93‑1 (MQ=255)
         aaTGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAAc                                                                              >  2:3186898/1‑93 (MQ=255)
          aTGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACg                                                                             >  1:3667143/1‑93 (MQ=255)
           tGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGc                                                                            >  2:1905737/1‑93 (MQ=255)
           tGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGc                                                                            <  1:1449856/93‑1 (MQ=255)
            ggCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCa                                                                           <  2:2269961/93‑1 (MQ=255)
            ggCCTGCTGACGCTATTGAAGTGCAACTGGCACAGGCAAACGGCGGATCTGCGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCa                                                                           >  1:985398/1‑93 (MQ=255)
             gCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCag                                                                          >  2:2324946/1‑93 (MQ=255)
               ctgctgACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCagag                                                                        >  1:1012597/1‑93 (MQ=255)
                tgctgACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAgaga                                                                       <  2:4439809/93‑1 (MQ=255)
                  ctgACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGaaa                                                                     <  1:1970426/93‑1 (MQ=255)
                    gACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatg                                                                   >  1:63035/1‑93 (MQ=255)
                    gACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatg                                                                   <  1:4101587/93‑1 (MQ=255)
                     gcGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatga                                                                  <  1:3440444/92‑1 (MQ=255)
                     aCGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatga                                                                  >  1:3092788/1‑93 (MQ=255)
                     aCGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatga                                                                  >  1:780889/1‑93 (MQ=255)
                     aCGCGATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatga                                                                  <  1:422225/93‑1 (MQ=255)
                      cGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatgat                                                                 >  1:4605991/1‑93 (MQ=255)
                       gcaaTTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatgatg                                                                >  1:2332715/4‑93 (MQ=255)
                       gCTATTGATGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatgatg                                                                >  2:4569860/1‑93 (MQ=255)
                          aTTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCaa                                                             <  1:802242/93‑1 (MQ=255)
                          aTTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCaa                                                             <  2:1137246/93‑1 (MQ=255)
                          aTTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCaa                                                             >  2:3054269/1‑93 (MQ=255)
                           ttGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAAt                                                            >  2:3678296/1‑93 (MQ=255)
                              aaGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAAtggt                                                         <  2:1397012/93‑1 (MQ=255)
                               aGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAAtggtg                                                        >  1:1426155/1‑93 (MQ=255)
                                gTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAAtggtgg                                                       <  2:220844/93‑1 (MQ=255)
                                gTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAAtggtgg                                                       >  2:58056/1‑93 (MQ=255)
                                 tGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTggg                                                      <  2:3496516/93‑1 (MQ=255)
                                  gCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTgggg                                                     >  2:4404571/1‑92 (MQ=255)
                                   cAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCg                                                    <  2:3530677/93‑1 (MQ=255)
                                    aaCTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCgg                                                   >  2:2458737/1‑93 (MQ=255)
                                     attGGAACATGCAAGCGGCGCATCTGTGCGTGCGATTTACAACCATGCTCAGCATCTCGATAAGCGCAGATAAATGATCGAATGGTGGGCgca                                                  <  2:1223311/91‑3 (MQ=255)
                                      cTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGAc                                                 <  1:4668643/93‑1 (MQ=255)
                                       tGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACt                                                >  2:4053894/1‑93 (MQ=255)
                                        ggCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTg                                               <  2:3923120/93‑1 (MQ=255)
                                          cacaTGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGc                                             >  1:1922151/1‑93 (MQ=255)
                                           acaTGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGCAATGATGCAATGGTGGGCGGACTGGCt                                            >  1:4431395/1‑93 (MQ=255)
                                           acaTGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCt                                            >  1:886859/1‑93 (MQ=255)
                                              tGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTtga                                         <  1:695364/93‑1 (MQ=255)
                                              tGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTtga                                         <  2:1582136/93‑1 (MQ=255)
                                              tGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTtga                                         >  2:2131982/1‑93 (MQ=255)
                                                 aaaCGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGGTtgatga                                      >  1:3586104/1‑93 (MQ=255)
                                                 aaaCGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTtgatga                                      <  1:3094749/93‑1 (MQ=255)
                                                 aaaCGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTtgatga                                      <  1:1148440/93‑1 (MQ=255)
                                                 aaaCGGCGGACCTGTGCGGGGGATTTACAACCATGCTAAGCATCTCGATAAACGCAAAGAAATGATGCAATGGTGGGCGGACTGGCTtgattg                                      >  2:1231418/1‑91 (MQ=255)
                                                   aCGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATgaga                                    <  2:4351765/93‑1 (MQ=255)
                                                      gcggATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAgg                                 >  2:738227/1‑93 (MQ=255)
                                                       cggATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTTATGAGAAggg                                >  2:2817511/1‑92 (MQ=255)
                                                       cggATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAggg                                >  2:1850495/1‑92 (MQ=255)
                                                       cggATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGt                                <  1:490230/93‑1 (MQ=255)
                                                         gATTTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAAGTgg                              >  1:2236318/1‑93 (MQ=255)
                                                         gATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGGTGAGAAGGTgg                              >  2:953772/1‑93 (MQ=255)
                                                         gATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTgg                              <  2:513932/93‑1 (MQ=255)
                                                           tCTGTGCGTGTGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGAGTGGCTTGATGAGAAGGTGGAg                            <  1:305926/93‑1 (MQ=255)
                                                              gtgCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGGTTGATGAGAAGGTGGAggtg                         >  2:3691163/1‑90 (MQ=255)
                                                              gtgCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGa                         <  1:4098844/93‑1 (MQ=255)
                                                              gtgCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGa                         <  2:1047891/93‑1 (MQ=255)
                                                               tgCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAg                        <  1:21339/93‑1 (MQ=255)
                                                                gCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGGGTGAgg                       >  1:4554815/1‑92 (MQ=255)
                                                                gCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGc                       <  2:1027240/93‑1 (MQ=255)
                                                                 cGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCg                      >  2:754768/1‑93 (MQ=255)
                                                                  gTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATTAGAAAGTGGAGTGAGCGa                     >  1:2930605/1‑93 (MQ=255)
                                                                  gTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGa                     >  1:2552918/1‑93 (MQ=255)
                                                                  gTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGAGTGAGCGa                     >  1:2580362/1‑93 (MQ=255)
                                                                   tGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCggc                    >  2:2732070/1‑91 (MQ=255)
                                                                   tGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGAc                    <  2:3792495/93‑1 (MQ=255)
                                                                     ggATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGAGGGAGCGACCt                  >  2:26216/1‑93 (MQ=255)
                                                                      gATTTACAACCATGCTCAGTATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTTATGAGAAGGGGGAGTGCGCGACCtt                 >  2:325045/1‑93 (MQ=255)
                                                                       aTTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTa                <  2:3926740/93‑1 (MQ=255)
                                                                        tttACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTaa               <  1:4398738/93‑1 (MQ=255)
                                                                        tttACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGGGGGAGCGACCTTaa               >  2:2181970/1‑93 (MQ=255)
                                                                           aCAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGAGGGAGCGACCTTaacaa            >  1:2479870/1‑93 (MQ=255)
                                                                           aCAACCATGCACATCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGGTTGATGAGAAGGTGGAGTGAGCGACCTTaacaa            <  1:1013022/93‑1 (MQ=255)
                                                                            cAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTaacaac           <  1:2851789/93‑1 (MQ=255)
                                                                             aaCCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTCATGAGGAGGGGGAGGGAGCGCCCGTGATAACt          >  1:745434/1‑93 (MQ=255)
                                                                              aCCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGCCTGGCTTGTTGAGAAAGTGGAGTTAGGCACCTTaacacata         >  1:1564055/1‑89 (MQ=255)
                                                                              aCCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGCCTAGATTTATGAGCAGGGCGAGAGAGCGAACCTAACAACtt         >  2:1411655/1‑92 (MQ=255)
                                                                              aCCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGGGAGCGACCTTAACAACTa         >  2:1082936/1‑93 (MQ=255)
                                                                               ccATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTAt        <  1:2163253/93‑1 (MQ=255)
                                                                                caagCTCAGCATCTCGATAAACGCAGAAAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATc       <  1:3748381/90‑1 (MQ=255)
                                                                                cATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATc       <  1:2026156/93‑1 (MQ=255)
                                                                                    cTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGGGTGAGCGACCTTAACAACTATCGAAt   >  2:286446/1‑93 (MQ=255)
                                                                                     tCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATa  <  1:3615564/93‑1 (MQ=255)
                                                                                     tCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGAGGGAGGGACCTTAACAACTATCGAATa  >  1:4261132/1‑93 (MQ=255)
                                                                                         |                                                                                        
ACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGTATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATA  >  NC_000913/2465568‑2465745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑