BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,488,381	A→G	I530I (ATT→ATC)	b2373 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,488,381	0	A	G	99.2%	369.6 / ‑4.5	117	I530I (ATT→ATC)	b2373	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (34/82); total (34/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

Only 100 of 118 total aligned reads displayed.

TTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCT  >  NC_000913/2488291‑2488470

ttGTTTTGGTTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGAt                                                                                         <  2:4673080/93‑1 (MQ=255)

 tGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATa                                                                                        <  2:3484463/93‑1 (MQ=255)

 tGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATa                                                                                        <  1:3233480/93‑1 (MQ=255)

   ttttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATAcc                                                                                      <  2:2229931/93‑1 (MQ=255)

   ttttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATAcc                                                                                      <  1:2454018/93‑1 (MQ=255)

    tttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCg                                                                                     <  1:3815069/93‑1 (MQ=255)

      tGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggt                                                                                   <  1:1049667/93‑1 (MQ=255)

        ggTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggtgg                                                                                 <  2:641755/93‑1 (MQ=255)

        ggTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggtgg                                                                                 <  2:185682/93‑1 (MQ=255)

        ggTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggtgg                                                                                 >  2:1989663/1‑93 (MQ=255)

         gTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggtggt                                                                                >  1:2128399/1‑93 (MQ=255)

           tAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTa                                                                              >  2:2005957/1‑93 (MQ=255)

             aGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTaaa                                                                            <  1:725735/93‑1 (MQ=255)

              gTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAg                                                                           <  1:680675/93‑1 (MQ=255)

              gTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAg                                                                           >  2:4202840/1‑93 (MQ=255)

                 tGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCAt                                                                        <  2:3059953/93‑1 (MQ=255)

                 tGGTAATATAGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCAt                                                                        <  1:3290015/93‑1 (MQ=255)

                  ggTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATg                                                                       <  2:2628957/93‑1 (MQ=255)

                  ggTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATg                                                                       >  1:1063163/1‑93 (MQ=255)

                   gTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGa                                                                      <  2:243940/93‑1 (MQ=255)

                    tAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGAc                                                                     <  2:542551/93‑1 (MQ=255)

                      atatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGa                                                                   <  1:1145939/93‑1 (MQ=255)

                       tatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGaa                                                                  >  2:1066963/1‑93 (MQ=255)

                         tGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGt                                                                >  1:4012311/1‑93 (MQ=255)

                         tGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGt                                                                <  2:1503740/93‑1 (MQ=255)

                          ggCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGtt                                                               <  2:4496834/93‑1 (MQ=255)

                           gCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTc                                                              <  1:3765148/93‑1 (MQ=255)

                            ccACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATCCCGGTGGTTAAAGCATGACGAAGTTCa                                                             <  2:275185/93‑1 (MQ=255)

                            ccACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCa                                                             <  2:4103982/93‑1 (MQ=255)

                            ccACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCa                                                             <  1:3651257/93‑1 (MQ=255)

                             cACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCAt                                                            >  1:2327110/1‑93 (MQ=255)

                             cACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCAt                                                            >  2:2946067/1‑93 (MQ=255)

                             cACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCAt                                                            >  2:3093110/1‑93 (MQ=255)

                              aCTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATc                                                           <  2:1189403/93‑1 (MQ=255)

                                  tCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTc                                                       <  1:113020/93‑1 (MQ=255)

                                   cAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCg                                                      <  1:1583951/93‑1 (MQ=255)

                                     gTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtg                                                    <  1:3355892/93‑1 (MQ=255)

                                       tCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggt                                                  <  2:3173225/93‑1 (MQ=255)

                                       tCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggt                                                  <  2:530938/93‑1 (MQ=255)

                                       tCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggt                                                  >  1:3128639/1‑93 (MQ=255)

                                         ctgctgCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGCCGAAGTTCATCTGTCGTGGTGa                                                >  2:809024/1‑93 (MQ=255)

                                         ctgctgCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGa                                                <  2:4069095/93‑1 (MQ=255)

                                          tgctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAc                                               <  1:3659264/93‑1 (MQ=255)

                                          tgctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAc                                               >  2:1451570/1‑93 (MQ=255)

                                           gctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACg                                              <  2:2421311/93‑1 (MQ=255)

                                               cAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtat                                          >  2:4440368/1‑93 (MQ=255)

                                                cgggTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtata                                         <  2:2748483/92‑1 (MQ=255)

                                                aGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtata                                         <  1:549409/93‑1 (MQ=255)

                                                aGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtata                                         >  2:1595835/1‑93 (MQ=255)

                                                aGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtata                                         <  1:3202354/93‑1 (MQ=255)

                                                 gggTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAg                                        >  2:3274905/1‑93 (MQ=255)

                                                 gggTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAg                                        >  1:4398862/1‑93 (MQ=255)

                                                  ggTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGc                                       <  1:4573747/93‑1 (MQ=255)

                                                  ggTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGc                                       <  2:1571312/93‑1 (MQ=255)

                                                   gTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGcc                                      <  2:236309/93‑1 (MQ=255)

                                                    tCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCa                                     >  2:2282385/1‑93 (MQ=255)

                                                    tCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCa                                     >  2:1308251/1‑93 (MQ=255)

                                                     cGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCaa                                    <  2:2160448/93‑1 (MQ=255)

                                                     cGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCaa                                    <  2:2486268/93‑1 (MQ=255)

                                                     cGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCaa                                    <  2:4174451/93‑1 (MQ=255)

                                                      gATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAAc                                   >  2:3527070/1‑93 (MQ=255)

                                                         gACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGcc                                >  1:2475706/1‑93 (MQ=255)

                                                          aCCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCa                               <  1:4173504/93‑1 (MQ=255)

                                                           ccACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCAc                              >  2:3596949/1‑93 (MQ=255)

                                                           ccACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCAc                              <  1:1851918/93‑1 (MQ=255)

                                                           ccACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCAc                              >  2:2633562/1‑93 (MQ=255)

                                                            cacaTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACg                             >  2:2388868/1‑93 (MQ=255)

                                                            cacaTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACg                             >  1:3003749/1‑93 (MQ=255)

                                                              caTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGaa                           >  1:1191571/1‑93 (MQ=255)

                                                               aTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGCCGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGaaa                          <  1:263891/93‑1 (MQ=255)

                                                                 taataaTGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACg                        >  1:676024/1‑93 (MQ=255)

                                                                   ataatTGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAAGCa                      >  1:1745325/1‑93 (MQ=255)

                                                                   ataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCa                      <  2:4511603/93‑1 (MQ=255)

                                                                     aatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATc                    <  2:118398/93‑1 (MQ=255)

                                                                      atGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATcc                   >  1:4027598/1‑93 (MQ=255)

                                                                       tgttCGTTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCa                  <  1:1755654/90‑1 (MQ=255)

                                                                       tGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAGGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCa                  <  2:4322813/93‑1 (MQ=255)

                                                                        ggTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCAt                 <  1:734281/93‑1 (MQ=255)

                                                                         gTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCAtt                <  1:3348795/93‑1 (MQ=255)

                                                                         gTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCAtt                >  1:3100355/1‑93 (MQ=255)

                                                                         gTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCAtt                <  1:4385476/93‑1 (MQ=255)

                                                                           cGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTaa              <  2:1067946/93‑1 (MQ=255)

                                                                           cGGTGTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTaa              <  2:1117218/93‑1 (MQ=255)

                                                                            ggTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAt             >  2:4443434/1‑93 (MQ=255)

                                                                            ggTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAt             <  2:2064083/93‑1 (MQ=255)

                                                                            ggTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAt             <  2:365866/93‑1 (MQ=255)

                                                                             gTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAtt            <  2:31116/93‑1 (MQ=255)

                                                                              tttGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAttt           <  1:2814615/93‑1 (MQ=255)

                                                                              tttGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAttt           <  2:3385069/93‑1 (MQ=255)

                                                                                 gcgcGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTc        <  2:3189177/93‑1 (MQ=255)

                                                                                  cgcgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCa       <  1:862025/93‑1 (MQ=255)

                                                                                    cgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATa     <  1:1180348/93‑1 (MQ=255)

                                                                                    cgACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATa     <  1:4570068/93‑1 (MQ=255)

                                                                                      aCTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAcc   >  2:3989639/1‑93 (MQ=255)

                                                                                      aCTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAcc   <  1:1827162/93‑1 (MQ=255)

                                                                                      aCTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAcc   <  1:3106863/93‑1 (MQ=255)

                                                                                      aCTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAcc   >  2:2913495/1‑93 (MQ=255)

                                                                                      aCTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAcc   <  1:1610382/93‑1 (MQ=255)

                                                                                       cTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCt  <  1:2389494/93‑1 (MQ=255)

                                                                                       cTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCt  <  2:809064/93‑1 (MQ=255)

TTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCT  >  NC_000913/2488291‑2488470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`