Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,505,495	C→T	Q140Q (CAG→CAA)	b2386 ←	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,505,495	0	C	T	100.0%	246.9 / NA	73	Q140Q (CAG→CAA)	b2386	putative transport protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (65/8);  total (65/8)

AATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGCTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAA  >  NC_000913/2505405‑2505583                                                                                            |                                                                                          aataatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTg                                                                                         >  1:233121/1‑93 (MQ=255)  ataatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTgg                                                                                        <  1:4298568/93‑1 (MQ=255)  ataatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTgg                                                                                        >  1:4410959/1‑93 (MQ=255)   taatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTggg                                                                                       >  1:3449539/1‑93 (MQ=255)   taatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTggg                                                                                       >  1:3853810/1‑93 (MQ=255)       cAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCTGTTGGG‑TAGa                                                                                  <  2:2868819/93‑1 (MQ=255)        aGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGaa                                                                                 >  1:3519058/1‑92 (MQ=255)        aGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGAc                                                                                 <  1:3916519/93‑1 (MQ=255)          cgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACAt                                                                               <  1:2880615/93‑1 (MQ=255)           gcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATc                                                                              <  2:3241438/93‑1 (MQ=255)           gcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATc                                                                              >  2:2587426/1‑93 (MQ=255)             aCCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCaa                                                                            >  1:2831708/1‑93 (MQ=255)               cAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAAcc                                                                          <  2:7569/93‑1 (MQ=255)                aaTGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAAccc                                                                         <  1:1066174/93‑1 (MQ=255)                aaTGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAAccc                                                                         >  2:947467/1‑93 (MQ=255)                 aTGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAAcccc                                                                        >  2:1259439/1‑92 (MQ=255)                  tGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACccgc                                                                       >  2:147235/1‑93 (MQ=255)                  tGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACccgc                                                                       <  2:3282908/93‑1 (MQ=255)                         ggACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATaaa                                                                >  1:2180704/1‑93 (MQ=255)                         ggACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATaaa                                                                >  2:334811/1‑93 (MQ=255)                          gACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATaaaa                                                               >  1:571746/1‑93 (MQ=255)                          gACGGCACCGGCGAAGAGGTCCCCCATTGCATAGTGCGGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATaaaa                                                               <  2:2894091/93‑1 (MQ=255)                           aCGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAAc                                                              >  1:2933724/1‑93 (MQ=255)                            ggccACCGGGGAGGAGGCCCCCTTTTGCATATGGCTGGGAGCGAGGTACAGCGGTGG‑GGTTGGTTGGG‑TAGACAAAAACCCGCCGATAAAAcc                                                             <  2:1334918/90‑1 (MQ=255)                             ggCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGACGATAAAACCa                                                            >  2:2149522/1‑93 (MQ=255)                             ggCACCGGCGAAGAGGTCGCCCATTGCATAGTGCGGGGATCGAAGTTCAGCAGTTG‑CGTCGGTGGGG‑TAGACATCACCCCGCCGATAAAACCa                                                            <  2:517934/93‑1 (MQ=255)                              gCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAg                                                           >  2:993800/1‑93 (MQ=255)                                aCCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGcc                                                         >  2:2805073/1‑93 (MQ=255)                                 ccGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCg                                                        >  1:2131466/1‑93 (MQ=255)                                   ggCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCggg                                                      >  2:2578620/1‑93 (MQ=255)                                   ggCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCggg                                                      >  1:1695437/1‑93 (MQ=255)                                    gCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGa                                                     >  2:3078722/1‑93 (MQ=255)                                     cGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGaa                                                    >  2:3536550/1‑93 (MQ=255)                                      gAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAAc                                                   >  2:1668532/1‑93 (MQ=255)                                      gAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGAGAAc                                                   >  1:3093280/1‑93 (MQ=255)                                      gAAGAGGTCGCCAATTGCATAGGGCTGGGATCGAAGTGCGGCAGTGG‑CGTGGGTGGGG‑TAGACATCACCCCGCCGATAAAACCAGCCGGGAAc                                                   <  2:766062/93‑1 (MQ=255)                                         gagGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGcc                                                >  1:4405204/1‑93 (MQ=255)                                          agGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCa                                               >  1:91266/1‑93 (MQ=255)                                          agGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCa                                               >  1:4580868/1‑93 (MQ=255)                                           ggTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAg                                              >  1:1405680/1‑93 (MQ=255)                                            gccccccTTTCCATGGTGCGGGAATCAACGTCCAGCATTTG‑CGTCGTTGGGG‑AACCCACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGt                                             <  2:3571492/89‑1 (MQ=255)                                            gccGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTGGGTGGGG‑TAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGt                                             <  2:3032877/91‑1 (MQ=255)                                            gTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGt                                             >  1:59344/1‑93 (MQ=255)                                            gTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGt                                             >  1:975992/1‑93 (MQ=255)                                            gTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGt                                             >  2:3482939/1‑93 (MQ=255)                                            gTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGAAATCAACCAGCCGATAAAACCAGCCGGGAACGCCAGt                                             >  2:1032563/1‑93 (MQ=255)                                              cccccATTCCATGGTGGGGGGATCGAAGTTCAGCAGTTG‑CGTGGTTGGGG‑AACACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGttt                                           <  1:2800851/91‑1 (MQ=255)                                               gCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTg                                          >  2:830158/1‑93 (MQ=255)                                                cctaTTACATAGTGCGGGGTTAAGGGTTAAGCGGTTG‑GGTCGGTGGGG‑TAGACCTCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGc                                         <  1:2789185/90‑1 (MQ=255)                                                cccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGc                                         >  1:4500662/1‑93 (MQ=255)                                                cccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGc                                         >  2:3428446/1‑93 (MQ=255)                                                 ccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGcc                                        >  1:93272/1‑93 (MQ=255)                                                 ccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCACTTTgtc                                        >  2:2281052/1‑91 (MQ=255)                                                 ccATTGAATAGGGTTGGGATCGAAGTTCAGCAGTTG‑CGTGGGTGGGG‑TAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGcc                                        <  2:632972/93‑1 (MQ=255)                                                  cATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCa                                       >  1:2088102/1‑93 (MQ=255)                                                  cATTGCATAGTGCTGGGATCGAAGTTCAGCAGCTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCa                                       >  1:2006036/1‑93 (MQ=255)                                                   aTTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAc                                      >  1:2692074/1‑93 (MQ=255)                                                   aTTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAc                                      >  2:2169013/1‑93 (MQ=255)                                                   aTTGAATAGTGCGGGGATCGAAGTTCAGCAGTTG‑CGTGGGTTGGG‑AAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAc                                      <  2:634178/93‑1 (MQ=255)                                                    ttGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAcc                                     >  2:568591/1‑93 (MQ=255)                                                      gCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGa                                   >  1:2854763/1‑93 (MQ=255)                                                      gCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGa                                   >  1:2754762/1‑93 (MQ=255)                                                       cATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGAt                                  >  2:1937835/1‑93 (MQ=255)                                                       cATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCAAGTTTGCCACCGAt                                  >  2:3008830/1‑93 (MQ=255)                                                        aTAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGTCACCGATa                                 >  1:403403/1‑93 (MQ=255)                                                        aTAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATa                                 >  2:3504254/1‑93 (MQ=255)                                                        aTAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATa                                 >  1:4386171/1‑93 (MQ=255)                                                        aTAGTGCTGGGAGAAAAGTTCAGAAGTTG‑CGTGGGTTGGG‑TACACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATa                                 <  1:1790599/93‑1 (MQ=255)                                                          aGTGCGGGGATCGAATTCCAGAAGTCG‑CGTCGGTGGGG‑TAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATaga                               <  2:3651886/93‑1 (MQ=255)                                                           gTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATagag                              >  2:3453460/1‑93 (MQ=255)                                                              cTGGGATAGAAGTTCAGCAGTTT‑CGTCGGTTGGG‑GAGACATCAATCCGCCGATAAAACCAGCCGGCGACGCCAGTTTGCCACCGATAGAGttt                           >  2:3701749/1‑92 (MQ=255)                                                               tGGGATCGAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCg                          >  1:3455682/1‑93 (MQ=255)                                                                  gATCGATGTTCAGC‑GTTGCCGTCGGTTGGG‑AAGACACCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCca                       <  1:4577447/93‑1 (MQ=255)                                                                   agcGAAGTGCAGGTGTTG‑CGT‑GGTTGGGATAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCcac                      <  1:2440973/91‑1 (MQ=255)                                                                      gAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaa                   >  2:3796884/1‑93 (MQ=255)                                                                      gAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaa                   >  1:3342523/1‑93 (MQ=255)                                                                      gAAGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaa                   >  1:4446655/1‑93 (MQ=255)                                                                       aaGTTGAGAGGTTG‑GGTGGGTGGGG‑AAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaaa                  <  1:2895289/93‑1 (MQ=255)                                                                       aaGTGCAGCAGTGG‑CGTGGGTGGGG‑AAAACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaaa                  <  2:1527933/93‑1 (MQ=255)                                                                        aGTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAag                 >  2:278062/1‑93 (MQ=255)                                                                         gTTCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagc                >  2:171168/1‑93 (MQ=255)                                                                          ttCAGCAGTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagca               >  2:2578242/1‑93 (MQ=255)                                                                            cagcatTTG‑CGTGGATTGGA‑TACACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagcagc             <  2:2162235/93‑1 (MQ=255)                                                                              gcagTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaa           >  1:1037544/1‑93 (MQ=255)                                                                              gcagTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaa           >  2:2260990/1‑93 (MQ=255)                                                                              gcagTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaa           >  2:2260989/1‑93 (MQ=255)                                                                              gcagTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaa           >  1:2856294/1‑93 (MQ=255)                                                                               gagTTG‑CGTGGTTTGGG‑TAAACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaaa          <  1:3569749/92‑1 (MQ=255)                                                                                agTGG‑CGTCGTTTGGT‑AACACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAAc         <  1:1493358/93‑1 (MQ=255)                                                                                 gTTG‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACa        >  1:3220875/1‑93 (MQ=255)                                                                                 gTTG‑CGTCGGTTGGG‑TAGACATCAACACGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACa        >  1:719643/1‑93 (MQ=255)                                                                                   tG‑CGTGGGTGGGG‑TAAACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATc      <  1:4675482/93‑1 (MQ=255)                                                                                   tG‑CGTGGGGTGGA‑TAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATc      <  1:1992015/93‑1 (MQ=255)                                                                                    g‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCg     >  1:4323905/1‑93 (MQ=255)                                                                                    g‑CGTCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCg     >  1:3145406/1‑93 (MQ=255)                                                                                        tCGGTTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGaa  >  2:2261338/1‑93 (MQ=255)                                                                                            |                                                                                          AATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTG‑CGTCGGCTGGG‑TAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAA  >  NC_000913/2505405‑2505583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑