BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	272,218	A→G	T45A (ACC→GCC)	ykfC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	272,218	0	A	G	95.9%	559.1 / 4.8	194	T45A (ACC→GCC)	ykfC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (4/4); new base G (93/93); total (97/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 202 total aligned reads displayed.

CTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGC  >  NC_000913/272128‑272307

cTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGcc                                                                                         >  2:773464/1‑93 (MQ=255)

cTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGcc                                                                                         >  1:3645940/1‑93 (MQ=255)

cTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGcc                                                                                         >  2:3778250/1‑93 (MQ=255)

cTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATAcc                                                                                         >  2:3967496/1‑93 (MQ=255)

 tACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGAGGCCCATGccc                                                                                        >  1:3581226/1‑93 (MQ=255)

  aCGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGcccc                                                                                       >  2:1212283/1‑93 (MQ=255)

  aCGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGcccc                                                                                       >  1:3985222/1‑93 (MQ=255)

     gATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCgg                                                                                    >  2:113395/1‑93 (MQ=255)

     gATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCgg                                                                                    <  2:1727516/93‑1 (MQ=255)

       ttCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCg                                                                                  >  1:3172261/1‑93 (MQ=255)

       ttCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGCGGGCCATGCCCCCGGCg                                                                                  >  1:4535488/1‑93 (MQ=255)

        tCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTTGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCAGCGt                                                                                 >  1:3405501/1‑93 (MQ=255)

         cAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGtt                                                                                <  2:1266584/93‑1 (MQ=255)

          aaCGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTg                                                                               <  1:3760651/93‑1 (MQ=255)

          aaCGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTg                                                                               >  1:1686984/1‑93 (MQ=255)

           aCGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGa                                                                              <  2:215791/93‑1 (MQ=255)

           aCGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGa                                                                              >  1:438047/1‑93 (MQ=255)

            cGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGAt                                                                             >  1:2393751/1‑93 (MQ=255)

            cGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGAt                                                                             <  1:579435/93‑1 (MQ=255)

             ggCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCGCGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATg                                                                            <  2:1998134/93‑1 (MQ=255)

             ggCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATg                                                                            <  2:1278996/93‑1 (MQ=255)

               ctgctgCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGc                                                                          <  1:2975722/93‑1 (MQ=255)

                tgctgcGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCg                                                                         >  2:2978501/1‑93 (MQ=255)

                tgctgcGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCg                                                                         <  2:2626817/93‑1 (MQ=255)

                tgctgcGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGACCATGCCCCCCGCGTTGATGGCg                                                                         >  2:249801/1‑93 (MQ=255)

                 gctgcGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGt                                                                        <  2:3937628/93‑1 (MQ=255)

                   tgcGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGa                                                                      >  1:1504383/1‑93 (MQ=255)

                   tgcGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGa                                                                      >  2:3269347/1‑93 (MQ=255)

                   tgcGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGa                                                                      <  2:4191811/93‑1 (MQ=255)

                   tgcGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGa                                                                      <  1:1861578/93‑1 (MQ=255)

                    gcGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaa                                                                     <  1:2773156/93‑1 (MQ=255)

                     cGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaac                                                                    <  2:390188/93‑1 (MQ=255)

                      gTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaaca                                                                   >  2:339013/1‑93 (MQ=255)

                      gTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaaca                                                                   >  1:3460512/1‑93 (MQ=255)

                       tCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATTACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGcacaa                                                                  >  2:2852122/1‑93 (MQ=255)

                       tCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaacaa                                                                  >  1:3816780/1‑93 (MQ=255)

                       tCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaacaa                                                                  >  2:4637753/1‑93 (MQ=255)

                       tCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaacaa                                                                  <  2:2782420/93‑1 (MQ=255)

                       tCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaacaa                                                                  >  1:1820979/1‑93 (MQ=255)

                        cTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaacaaa                                                                 >  1:1998996/1‑93 (MQ=255)

                         tGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaacaaaa                                                                <  2:2906177/93‑1 (MQ=255)

                         tGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaacaaaa                                                                >  2:147102/1‑93 (MQ=255)

                         tGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaacaaaa                                                                >  1:1278459/1‑93 (MQ=255)

                           aTAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaacaaaaca                                                              >  1:3337542/1‑93 (MQ=255)

                            tAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGaacaaaacaa                                                             <  1:2703372/93‑1 (MQ=255)

                             aaCGCAGCCAGAATGGCTGGCTGAAGCTGCGCGGATCACGCTTTCATCAAAGGGGGCACATACCCCCGGCGTTGATGGCGTGAACAAAACAAt                                                            <  2:2372842/93‑1 (MQ=255)

                             aaCACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAAt                                                            >  1:4487178/1‑93 (MQ=255)

                               cacaACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGc                                                          <  1:928788/93‑1 (MQ=255)

                                acaACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCt                                                         >  2:3886875/1‑93 (MQ=255)

                                 caACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGAACAAAACAATGCTa                                                        <  2:2070533/93‑1 (MQ=255)

                                  aaCCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTAc                                                       <  1:551130/93‑1 (MQ=255)

                                  aaCCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTAc                                                       <  1:879338/93‑1 (MQ=255)

                                   aCCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACa                                                      >  1:425724/1‑93 (MQ=255)

                                   aCCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACa                                                      <  1:2608002/93‑1 (MQ=255)

                                   aCCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACa                                                      <  2:4511411/93‑1 (MQ=255)

                                    ccAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAg                                                     >  2:1830924/1‑93 (MQ=255)

                                     cAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAgg                                                    <  2:3635900/93‑1 (MQ=255)

                                           ggctggctGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGAc                                              <  2:3078428/93‑1 (MQ=255)

                                           ggctggctGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGAc                                              <  1:988977/93‑1 (MQ=255)

                                            gctggctgAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACt                                             >  2:2687417/1‑93 (MQ=255)

                                             ctggctgAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTg                                            <  1:4063617/93‑1 (MQ=255)

                                                  tgAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGt                                       >  1:1177914/1‑93 (MQ=255)

                                                   gAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGtt                                      <  2:4097147/93‑1 (MQ=255)

                                                   gAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGtt                                      >  1:603455/1‑93 (MQ=255)

                                                   gAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTCGCTGtt                                      >  1:486671/1‑93 (MQ=255)

                                                       cggcgCGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGc                                  <  2:3557436/93‑1 (MQ=255)

                                                         gcgcGGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTg                                >  2:252836/1‑93 (MQ=255)

                                                          cgcgGATCACGCTGTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGc                               <  2:1606420/93‑1 (MQ=255)

                                                           gcgGATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCa                              <  1:3186721/93‑1 (MQ=255)

                                                             ggATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCaaa                            <  2:428694/93‑1 (MQ=255)

                                                             ggATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCaaa                            >  1:2167710/1‑93 (MQ=255)

                                                             ggATCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCaaa                            <  1:3348357/93‑1 (MQ=255)

                                                               aTCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATc                          >  1:3425156/1‑93 (MQ=255)

                                                                tCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATcc                         >  1:1228547/1‑93 (MQ=255)

                                                                tCACGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATcc                         >  1:4588577/1‑93 (MQ=255)

                                                                  aCGCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTc                       >  2:1939595/1‑93 (MQ=255)

                                                                    gCTTTCATCAAAGGGGGCCCATGCCCGCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAg                     <  2:1642942/93‑1 (MQ=255)

                                                                    gCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAg                     <  2:4414335/93‑1 (MQ=255)

                                                                    gCTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAg                     <  1:3100972/93‑1 (MQ=255)

                                                                     cTTTCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGGTGAGCTGCAAATCCTCAgg                    >  2:3039766/1‑93 (MQ=255)

                                                                       ttCATCAAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGa                  <  1:2871494/93‑1 (MQ=255)

                                                                        tcatcaAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGAt                 <  1:1971323/93‑1 (MQ=255)

                                                                          atcaAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGa               <  1:1867310/93‑1 (MQ=255)

                                                                          atcaAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGa               <  1:745202/93‑1 (MQ=255)

                                                                           tcaAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGaa              >  1:1005594/1‑93 (MQ=255)

                                                                           tcaAAGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGaa              <  2:1509130/93‑1 (MQ=255)

                                                                             aaaGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAAtt            <  2:1477761/93‑1 (MQ=255)

                                                                              aaGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTa           >  2:628614/1‑93 (MQ=255)

                                                                               aGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTAc          >  1:3684183/1‑93 (MQ=255)

                                                                               aGGGGGCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTAc          >  1:1561540/1‑93 (MQ=255)

                                                                                  gggCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTActct       >  2:3588025/1‑93 (MQ=255)

                                                                                   ggCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACtctc      <  2:2339136/93‑1 (MQ=255)

                                                                                    gCCCATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCa     <  2:880373/93‑1 (MQ=255)

                                                                                     cccATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGTCTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTTCTCTCAg    <  2:4342039/93‑1 (MQ=255)

                                                                                     cccATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAg    <  2:1448697/93‑1 (MQ=255)

                                                                                     cccATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAg    <  2:4138554/93‑1 (MQ=255)

                                                                                      ccATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAgg   >  2:1741988/1‑93 (MQ=255)

                                                                                      ccATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAgg   <  1:2039211/93‑1 (MQ=255)

                                                                                      ccATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAgg   <  1:2929652/93‑1 (MQ=255)

                                                                                       cATGCCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGc  >  1:2359081/1‑93 (MQ=255)

CTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGC  >  NC_000913/272128‑272307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`