BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,564,113	G→A	G264G (GGC→GGT)	b2451 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,564,113	0	G	A	99.2%	466.2 / NA	131	G264G (GGC→GGT)	b2451	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (61/69); minor base T (0/1); total (61/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

CGGATGGTCATGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCC  >  NC_000913/2564023‑2564202

cgGATGGTCATGCAGCGCAGTTGCAAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCAcacc                                                                                         <  1:1222469/93‑1 (MQ=255)

 ggATGGTCATGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCAcaccg                                                                                        >  1:890613/1‑93 (MQ=255)

 ggATGGTCATGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCAcaccc                                                                                        >  2:2544427/1‑92 (MQ=255)

  gATGGTCATGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCAcaccgc                                                                                       <  2:4308365/93‑1 (MQ=255)

  gATGGTCATGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCAcaccgc                                                                                       >  2:3300135/1‑93 (MQ=255)

    tGGTCATGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCAGGTGGCGATAACATTCGCCCACACCGCCa                                                                                     >  2:3047121/1‑93 (MQ=255)

    tGGTCATGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAAAGGGTCGGCGGGCGGGTGGCGATAACATTCGTCCACACCCCCa                                                                                     >  1:2615620/1‑93 (MQ=255)

     ggTCATGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAg                                                                                    <  1:1335183/93‑1 (MQ=255)

      gTCATGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGa                                                                                   >  1:1462441/1‑93 (MQ=255)

       tCATGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCCGGGGGGGATAACATTCGCCCACACCGCCAgga                                                                                  >  1:391599/1‑91 (MQ=255)

        cATGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGaaa                                                                                 <  1:2865817/93‑1 (MQ=255)

         aTGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAg                                                                                >  1:4337105/1‑93 (MQ=255)

         aTGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAg                                                                                >  1:3882680/1‑93 (MQ=255)

          tGCAGCGCAGTTGCCAGCAGCGTGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGc                                                                               <  2:2290457/93‑1 (MQ=255)

          tGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGc                                                                               <  2:2677226/93‑1 (MQ=255)

           gCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGGAAGCg                                                                              >  1:919151/1‑93 (MQ=255)

            cAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGt                                                                             <  1:1863722/93‑1 (MQ=255)

             aGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTa                                                                            <  2:2039567/93‑1 (MQ=255)

              gcgcAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTaa                                                                           >  1:3959989/1‑93 (MQ=255)

               cgcAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAAt                                                                          >  1:355764/1‑93 (MQ=255)

               cgcAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAAt                                                                          <  1:3145929/93‑1 (MQ=255)

                gcAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATg                                                                         >  1:778537/1‑93 (MQ=255)

                gcAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATg                                                                         <  1:1301958/93‑1 (MQ=255)

                 cAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGa                                                                        >  1:2474753/1‑93 (MQ=255)

                  aGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGAt                                                                       >  1:1276224/1‑93 (MQ=255)

                   gctGCCAGCAGCGGGCTAATATCGGCAAAACAGAGCGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACTCCGCCAGAAAGCGTAATGAtt                                                                      <  1:131132/91‑1 (MQ=255)

                   gTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGAtt                                                                      >  2:1080364/1‑93 (MQ=255)

                   gTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGAtt                                                                      >  2:4555231/1‑93 (MQ=255)

                    ttGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGAttt                                                                     >  2:3619699/1‑93 (MQ=255)

                     tGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTc                                                                    <  2:2921161/93‑1 (MQ=255)

                      gCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCg                                                                   <  1:502846/93‑1 (MQ=255)

                       ccAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCgg                                                                  <  2:4478883/93‑1 (MQ=255)

                       ccAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCT‑GTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCggg                                                                 <  2:3025408/93‑1 (MQ=255)

                        cagcagCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCggg                                                                 <  2:2444054/93‑1 (MQ=255)

                        cagcagCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCggg                                                                 >  2:3819238/1‑93 (MQ=255)

                         agcagcGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGc                                                                >  2:184299/1‑93 (MQ=255)

                         agcagcGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGc                                                                >  2:3164758/1‑93 (MQ=255)

                          gcagcGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCg                                                               >  1:3492602/1‑93 (MQ=255)

                          gcagcGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCg                                                               >  1:1367910/1‑93 (MQ=255)

                           cagcGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGt                                                              >  1:708629/1‑93 (MQ=255)

                            agcGGGCCAATATCGGAAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTa                                                             <  2:3574702/93‑1 (MQ=255)

                             gcGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTaa                                                            >  1:3963829/1‑93 (MQ=255)

                              cGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAac                                                           <  1:3343427/93‑1 (MQ=255)

                                 gCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACAcc                                                        <  2:4053514/93‑1 (MQ=255)

                                    aaTATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGc                                                     <  2:4724/93‑1 (MQ=255)

                                      tatCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCgg                                                   <  2:2332368/93‑1 (MQ=255)

                                      tatCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCgg                                                   >  1:4105435/1‑93 (MQ=255)

                                          ggCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGgcag                                               >  2:1440698/1‑93 (MQ=255)

                                           gCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGgcagc                                              >  1:2300558/1‑93 (MQ=255)

                                           gCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGgcagc                                              <  2:1479566/93‑1 (MQ=255)

                                             aaaaCAGAACGGGTCGGCGGGCTGGTGGCGATAAAATTCGGCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCaa                                            >  2:2525409/1‑93 (MQ=255)

                                               aaCAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAAc                                          <  1:2005618/93‑1 (MQ=255)

                                               aaCAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAAc                                          >  1:3690631/1‑93 (MQ=255)

                                                aCAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAAcc                                         >  2:360260/1‑93 (MQ=255)

                                                aCAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAAcc                                         <  2:3135095/93‑1 (MQ=255)

                                                aCAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAAcc                                         >  2:3839407/1‑93 (MQ=255)

                                                aCAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAAcc                                         <  1:2310369/93‑1 (MQ=255)

                                                aCAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAAcc                                         <  1:2517750/93‑1 (MQ=255)

                                                  aGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCgg                                       <  1:4318206/93‑1 (MQ=255)

                                                   gAACGGTGCGGCGGCCTGGTGGCGATCACATTCTCCCGCACCGCCAGAAAGCGTAATGACTTCGGGCGTAACACCTGCGGGCAGCAAACCGGt                                      <  1:2128981/93‑1 (MQ=255)

                                                   gAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGt                                      <  2:189718/93‑1 (MQ=255)

                                                    aaCGGGTCGGCGGGCTGGTTGCGATAACATTCGCCCACCCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGtt                                     <  1:1320952/93‑1 (MQ=255)

                                                    aaCGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGtt                                     <  1:3108863/93‑1 (MQ=255)

                                                    aaCGGGTCGGCCGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGtt                                     <  1:1313943/93‑1 (MQ=255)

                                                        ggccggcggGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCa                                 >  2:2216548/4‑93 (MQ=255)

                                                        ggTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCa                                 <  2:3443272/93‑1 (MQ=255)

                                                        ggTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCa                                 >  2:2533598/1‑93 (MQ=255)

                                                        ggTCGGCGGGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCa                                 <  2:4430526/93‑1 (MQ=255)

                                                         gTCGGCGGGCTGGTGGCGAGAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCAt                                <  1:1760850/93‑1 (MQ=255)

                                                            ggcggGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCaa                             <  2:735352/93‑1 (MQ=255)

                                                             gcggGCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAAt                            <  1:2334841/93‑1 (MQ=255)

                                                                ggCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGcc                         >  2:2113449/1‑93 (MQ=255)

                                                                ggCGGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGcc                         <  2:4116040/93‑1 (MQ=255)

                                                                 gCTGGTGGCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCt                        <  2:2216419/93‑1 (MQ=255)

                                                                   tggtggCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTgc                      <  1:356117/93‑1 (MQ=255)

                                                                    ggtggCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTgcg                     <  1:3760044/93‑1 (MQ=255)

                                                                     gtggCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGTTTTGCATCAATGCCTgcgc                    <  2:4545911/93‑1 (MQ=255)

                                                                     gtggCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTgcgc                    <  1:1499739/93‑1 (MQ=255)

                                                                       ggCGATAACATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGa                  <  2:3568894/93‑1 (MQ=255)

                                                                           aTAACATTCGCCCACACCGCCAGAAAGCGTAACGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGCGCgg              >  1:2109953/1‑93 (MQ=255)

                                                                             aaCATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTg            >  2:2787483/1‑93 (MQ=255)

                                                                              aCATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTga           >  2:3082138/1‑93 (MQ=255)

                                                                              aCATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTga           <  2:3248284/93‑1 (MQ=255)

                                                                              aCATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTga           >  2:403722/1‑93 (MQ=255)

                                                                              aCATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGCga           >  2:1579503/1‑93 (MQ=255)

                                                                               cATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTgag          >  1:2893720/1‑93 (MQ=255)

                                                                               cATTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTgag          <  1:734074/93‑1 (MQ=255)

                                                                                aTTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTgaga         <  1:4056499/93‑1 (MQ=255)

                                                                                aTTCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTgaga         <  1:385898/93‑1 (MQ=255)

                                                                                 ttCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGagag        >  2:320271/1‑93 (MQ=255)

                                                                                 ttCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGagag        <  1:2615267/93‑1 (MQ=255)

                                                                                 ttCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGATGagag        <  2:681445/93‑1 (MQ=255)

                                                                                  tCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACTCCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGc       <  2:1345822/93‑1 (MQ=255)

                                                                                  tCGCCCACACCGCCAGAAAGCGTAATGATTTCGGGCGGAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGc       <  1:546239/93‑1 (MQ=255)

                                                                                     cccACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGtt    >  2:3999612/1‑93 (MQ=255)

                                                                                     cccACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGtt    <  1:2242866/93‑1 (MQ=255)

                                                                                     cccACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGtt    >  1:3631072/1‑93 (MQ=255)

                                                                                      ccACACCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGCTc   >  1:1670291/1‑93 (MQ=255)

                                                                                       cacaCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTcc  <  2:4248969/93‑1 (MQ=255)

                                                                                       cacaCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTcc  <  2:601724/93‑1 (MQ=255)

CGGATGGTCATGCAGCGCAGTTGCCAGCAGCGGGCCAATATCGGCAAAACAGAACGGGTCGGCGGGCTGGTGGCGATAACATTCGCCCACGCCGCCAGAAAGCGTAATGATTTCGGGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCC  >  NC_000913/2564023‑2564202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`