BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,644,497	A→G	R283R (CGT→CGC)	pbpC ←	putative peptidoglycan enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,644,497	0	A	G	100.0%	555.7 / NA	174	R283R (CGT→CGC)	pbpC	putative peptidoglycan enzyme
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (85/89); total (85/89)

Only 100 of 178 total aligned reads displayed.

AACACGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTG  >  NC_000913/2644407‑2644586

aaCACGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcg                                                                                         >  2:4210803/1‑93 (MQ=255)

 acacGCATATCGGTATGATCACCCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcgt                                                                                        <  2:3786417/93‑1 (MQ=255)

 acacGCATATCGATATGACCACCCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcgt                                                                                        <  2:2815250/93‑1 (MQ=255)

   acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcgtcg                                                                                      >  1:4146881/1‑93 (MQ=255)

   acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcgtcg                                                                                      <  2:1049269/93‑1 (MQ=255)

   acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcgtcg                                                                                      <  2:526906/93‑1 (MQ=255)

     gCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGtt                                                                                    <  2:3432737/93‑1 (MQ=255)

      cATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCCCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTg                                                                                   <  2:114943/93‑1 (MQ=255)

       atatCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGa                                                                                  >  1:611029/1‑93 (MQ=255)

       atatCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGa                                                                                  >  1:675566/1‑93 (MQ=255)

         atCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAg                                                                                >  2:427455/1‑93 (MQ=255)

         atCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAg                                                                                >  2:172540/1‑93 (MQ=255)

          tCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGa                                                                               >  1:3810991/1‑93 (MQ=255)

          tCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGa                                                                               <  2:2362668/93‑1 (MQ=255)

             gTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCg                                                                            <  1:3386439/93‑1 (MQ=255)

                 gATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCAt                                                                        >  1:922170/1‑93 (MQ=255)

                   tCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATcc                                                                      >  2:3963214/1‑93 (MQ=255)

                     aaCCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCGCTTTCCAGTTTTGCGCCAGGTCTTCCAGGCGTCGTTGAAGACCGGCATCCaa                                                                    >  1:1123817/1‑93 (MQ=255)

                      aCCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAAc                                                                   <  1:1194209/93‑1 (MQ=255)

                      aCCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAAc                                                                   >  1:4227178/1‑93 (MQ=255)

                      aCCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAAc                                                                   >  2:326156/1‑93 (MQ=255)

                      aCCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAAc                                                                   <  1:1010647/93‑1 (MQ=255)

                       ccACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACg                                                                  >  1:4669670/1‑93 (MQ=255)

                       ccACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACg                                                                  >  2:804456/1‑93 (MQ=255)

                       ccACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACg                                                                  >  1:2332994/1‑93 (MQ=255)

                         ccGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgta                                                                <  2:147990/92‑1 (MQ=255)

                         aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgta                                                                >  2:2555409/1‑93 (MQ=255)

                          cGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgtag                                                               <  1:4333195/93‑1 (MQ=255)

                          cGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgtag                                                               >  1:589027/1‑93 (MQ=255)

                           gATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgtagt                                                              >  2:1417426/1‑93 (MQ=255)

                           gATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgtagt                                                              <  2:1825926/93‑1 (MQ=255)

                            atcatcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgtagta                                                             >  1:810227/1‑93 (MQ=255)

                            atcatcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgtagta                                                             <  2:2483056/93‑1 (MQ=255)

                             tcatcGCGAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGtattag                                                            <  2:1919157/93‑1 (MQ=255)

                              catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTagtagt                                                           <  2:567068/93‑1 (MQ=255)

                              catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTagtagt                                                           >  2:2009364/1‑93 (MQ=255)

                               atcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTg                                                          >  1:18516/1‑93 (MQ=255)

                               atcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTg                                                          >  2:2858194/1‑93 (MQ=255)

                                tcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGa                                                         >  1:3373611/1‑93 (MQ=255)

                                  gCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGAtt                                                       >  2:3552413/1‑93 (MQ=255)

                                  gCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGAtt                                                       >  2:217126/1‑93 (MQ=255)

                                   ccAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGAttt                                                      >  1:1017322/1‑93 (MQ=255)

                                   ccAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGAttt                                                      >  1:2587724/1‑93 (MQ=255)

                                   ccAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGAttt                                                      >  1:220822/1‑93 (MQ=255)

                                   ccAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGAttt                                                      <  2:3345223/93‑1 (MQ=255)

                                   ccAGTGAGCTGCGCGGTGGCAACCGCCATTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGAttt                                                      >  1:387689/1‑93 (MQ=255)

                                    cAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGAtttt                                                     <  1:438524/93‑1 (MQ=255)

                                    cAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGAtttt                                                     >  2:1364078/1‑93 (MQ=255)

                                     aGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTg                                                    <  2:3105649/93‑1 (MQ=255)

                                         aGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgc                                                <  2:1071614/93‑1 (MQ=255)

                                          gCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgct                                               <  2:3932546/93‑1 (MQ=255)

                                          gCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgct                                               <  1:34243/93‑1 (MQ=255)

                                            tGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttt                                             <  2:2124038/93‑1 (MQ=255)

                                            tGCGCGGGGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttt                                             <  1:2756298/93‑1 (MQ=255)

                                             tcggggTGGCAACCGCACTTTCCAGTTTTGCTCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCACCGTAGTAGTGATTTTGTCgctttt                                            <  2:4294352/89‑1 (MQ=255)

                                              cgcgGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttg                                           <  2:3521992/93‑1 (MQ=255)

                                                cgGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttgct                                         <  1:2062358/93‑1 (MQ=255)

                                                cgGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttgct                                         <  1:1398111/93‑1 (MQ=255)

                                                 ggTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttgctt                                        <  2:3962856/93‑1 (MQ=255)

                                                 ggTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttgctt                                        <  2:1322041/93‑1 (MQ=255)

                                                  gTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttgcttt                                       >  2:1404950/1‑93 (MQ=255)

                                                   tGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttgctttt                                      <  1:2599810/93‑1 (MQ=255)

                                                   tGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttgctttt                                      >  2:4569717/1‑93 (MQ=255)

                                                   tGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttgctttt                                      >  1:1668767/1‑93 (MQ=255)

                                                        aCCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGa                                 <  1:297584/93‑1 (MQ=255)

                                                         ccGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAg                                <  1:4236150/93‑1 (MQ=255)

                                                          cGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGc                               >  1:3289097/1‑93 (MQ=255)

                                                            cccTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcat                             >  2:2405996/1‑93 (MQ=255)

                                                            cccTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcat                             >  2:50368/1‑93 (MQ=255)

                                                              cTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcatca                           <  1:106391/93‑1 (MQ=255)

                                                               tttCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcatcat                          >  1:3001167/1‑93 (MQ=255)

                                                               tttCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcatcat                          <  2:4144526/93‑1 (MQ=255)

                                                                ttCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATg                         <  1:3138618/93‑1 (MQ=255)

                                                                ttCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATg                         >  2:3375853/1‑93 (MQ=255)

                                                                 tCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATgc                        <  2:1680978/93‑1 (MQ=255)

                                                                  ccAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATgcg                       >  2:3067193/1‑93 (MQ=255)

                                                                   cAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATgcgc                      >  1:2785398/1‑93 (MQ=255)

                                                                    aGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGcgcg                     >  2:1610240/1‑93 (MQ=255)

                                                                     gTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGa                    >  1:4656860/1‑93 (MQ=255)

                                                                       tttGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGaaa                  <  1:1481305/93‑1 (MQ=255)

                                                                        ttGCGCCAGTTCTTCCAGGCGTCGTTGAAGCCCGGCATCCAACGTAGTAGTGAATTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGaaaa                 <  1:3422212/93‑1 (MQ=255)

                                                                        ttGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGaaaa                 <  2:3126912/93‑1 (MQ=255)

                                                                         tGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAAc                <  2:2717907/93‑1 (MQ=255)

                                                                         tGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAAc                <  1:3957646/93‑1 (MQ=255)

                                                                            gcCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGc             <  2:1098056/93‑1 (MQ=255)

                                                                              cAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCgg           >  1:188862/1‑93 (MQ=255)

                                                                               aGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGt          <  1:2881320/93‑1 (MQ=255)

                                                                               aGTTCTTCCAGGCATCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGt          >  2:4503487/1‑93 (MQ=255)

                                                                                gTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTg         >  1:3488321/1‑93 (MQ=255)

                                                                                gTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTg         >  1:2462999/1‑93 (MQ=255)

                                                                                 ttcttcCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGc        >  1:4005424/1‑93 (MQ=255)

                                                                                 ttcttcCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGc        >  1:1793869/1‑93 (MQ=255)

                                                                                   cttcCAGGCGTCGTTGAAGACAGGCATCCAACGTAGAAGTGATATTGTGGCTTTTGCTTTTACCGAGCATAATGCGCGAAAACAGCGGTGACa      >  2:3480049/1‑93 (MQ=255)

                                                                                    ttcCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAg     >  2:1592504/1‑93 (MQ=255)

                                                                                    ttcCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAg     >  1:3499099/1‑93 (MQ=255)

                                                                                    ttcCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAg     >  2:78527/1‑93 (MQ=255)

                                                                                     tcCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGt    <  1:1058556/93‑1 (MQ=255)

                                                                                      ccAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGtt   <  1:869018/93‑1 (MQ=255)

                                                                                       cAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTg  >  2:2012757/1‑93 (MQ=255)

                                                                                       cAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTg  >  2:874222/1‑93 (MQ=255)

AACACGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTG  >  NC_000913/2644407‑2644586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`