BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,675,068	T→C	E438E (GAA→GAG)	yphE ←	putative ATP‑binding component of a transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,675,068	0	T	C	100.0%	485.2 / NA	154	E438E (GAA→GAG)	yphE	putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (68/86); total (68/86)

Only 100 of 157 total aligned reads displayed.

GGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGAT  >  NC_000913/2674979‑2675156

ggCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGGAAATCTGCTGTTTGGCCTCg                                                                                       >  1:2920283/1‑93 (MQ=255)

 gCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGa                                                                                      <  2:4628914/93‑1 (MQ=255)

  cAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGat                                                                                     <  1:2634595/93‑1 (MQ=255)

  cAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGat                                                                                     <  1:1706137/93‑1 (MQ=255)

  cAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGat                                                                                     >  1:2306540/1‑93 (MQ=255)

   aaCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGata                                                                                    <  2:2603582/93‑1 (MQ=255)

    aCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGatat                                                                                   <  1:182624/93‑1 (MQ=255)

    aCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGatat                                                                                   >  2:3008228/1‑93 (MQ=255)

     ctcctcCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATc                                                                                  <  1:4085583/93‑1 (MQ=255)

     ctcctcCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATc                                                                                  >  2:1840490/1‑93 (MQ=255)

     ctcctcCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATc                                                                                  <  1:2557135/93‑1 (MQ=255)

       cctccACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGa                                                                                <  1:1001895/93‑1 (MQ=255)

        ctccACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAc                                                                               <  1:958865/93‑1 (MQ=255)

        ctccACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAc                                                                               >  1:3294623/1‑93 (MQ=255)

            ccTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAcgccg                                                                           <  1:1298255/92‑1 (MQ=255)

            aCTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAcgccg                                                                           <  1:2143809/93‑1 (MQ=255)

            aCTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAcgccg                                                                           >  2:339362/1‑93 (MQ=255)

            aCTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAcgccg                                                                           >  1:1414709/1‑93 (MQ=255)

              ttCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCcgcg                                                                         <  1:1298985/93‑1 (MQ=255)

                 ccTGGAGATGAACCCCACGCTTTTCCCGTCGGCAGCCAGCTCACGGACAATACGGTACATCTGCTGTTTCGCCTCGATATCGACGCCGCGCGt                                                                      <  2:4351496/92‑1 (MQ=255)

                 aCTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGt                                                                      >  1:161544/1‑93 (MQ=255)

                  cTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGtt                                                                     <  2:1407104/93‑1 (MQ=255)

                  cTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGtt                                                                     >  2:2015004/1‑93 (MQ=255)

                  cTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCAAGGACAATACGGTAAATCTGCTGTTTGGACTCGATATCGACGACGCGCGtt                                                                     >  2:2015003/1‑93 (MQ=255)

                     gagaTAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGc                                                                  <  1:3753867/93‑1 (MQ=255)

                      agaTAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCt                                                                 <  2:422879/93‑1 (MQ=255)

                       gaTAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCtc                                                                <  2:4395665/93‑1 (MQ=255)

                          aaaCACCCCGCTTTTTCCTTCGTCAGCCAGCTCGCGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCtcgtc                                                             <  2:3063050/93‑1 (MQ=255)

                           aaCACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCtcgtcg                                                            >  1:247023/1‑93 (MQ=255)

                           aaCACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCtcgtcg                                                            >  2:771116/1‑93 (MQ=255)

                            acacCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGa                                                           <  2:1987826/93‑1 (MQ=255)

                            acacCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGa                                                           >  2:1696217/1‑93 (MQ=255)

                             caccacGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAg                                                          >  2:3989114/1‑93 (MQ=255)

                             caccacGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAg                                                          <  1:19028/93‑1 (MQ=255)

                              cccacGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGc                                                         <  2:2239551/92‑1 (MQ=255)

                               ccacGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGca                                                        <  1:317931/93‑1 (MQ=255)

                               ccacGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGca                                                        >  2:4637670/1‑93 (MQ=255)

                                  cGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGcaaca                                                     >  1:1012692/1‑93 (MQ=255)

                                   gCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGcaacaa                                                    <  2:1377344/93‑1 (MQ=255)

                                      ttttCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAAt                                                 <  1:4490241/93‑1 (MQ=255)

                                      ttttCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAAt                                                 <  2:2324960/93‑1 (MQ=255)

                                       tttCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATc                                                <  2:645005/93‑1 (MQ=255)

                                        ttCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATct                                               <  1:2831420/93‑1 (MQ=255)

                                        ttCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATct                                               <  2:747447/93‑1 (MQ=255)

                                        ttCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATct                                               <  1:4047468/93‑1 (MQ=255)

                                         tCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctg                                              <  1:2127182/93‑1 (MQ=255)

                                         tCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctg                                              >  2:282741/1‑93 (MQ=255)

                                          ccTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctgg                                             <  1:1342223/93‑1 (MQ=255)

                                           cTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctggc                                            <  2:699456/93‑1 (MQ=255)

                                            ttCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctggct                                           <  1:676735/93‑1 (MQ=255)

                                            ttCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGACGCGAGTTGGCTCGTCGAGCTACAAAAAccggcg                                           >  2:407184/1‑92 (MQ=255)

                                             tCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctggctg                                          <  1:4134534/93‑1 (MQ=255)

                                             tCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctggctg                                          <  2:876361/93‑1 (MQ=255)

                                                gCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGgca                                       >  2:692475/1‑93 (MQ=255)

                                                  agccagcTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGgcagc                                     <  1:442574/93‑1 (MQ=255)

                                                     cagcTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATa                                  <  1:4348666/93‑1 (MQ=255)

                                                     cagcTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATa                                  <  2:544021/93‑1 (MQ=255)

                                                       gcTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGa                                >  1:1439771/1‑93 (MQ=255)

                                                       gcTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGa                                <  2:4442558/93‑1 (MQ=255)

                                                        cTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAc                               <  2:970004/93‑1 (MQ=255)

                                                        cTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAc                               <  2:173205/93‑1 (MQ=255)

                                                        cTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAc                               <  1:498557/93‑1 (MQ=255)

                                                         tCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAcc                              <  2:1456518/93‑1 (MQ=255)

                                                         tCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAcc                              <  2:356500/93‑1 (MQ=255)

                                                         tCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAcc                              >  1:2234964/1‑93 (MQ=255)

                                                         tCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAcc                              <  1:2091575/93‑1 (MQ=255)

                                                         ccACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAcc                              <  2:3351478/92‑1 (MQ=255)

                                                          cACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAccc                             >  2:86317/1‑93 (MQ=255)

                                                            cgtaCAATACGGTAAATCTGCTGTGTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCaa                           <  2:2599896/90‑1 (MQ=255)

                                                             ggACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAAc                          >  1:1608267/1‑93 (MQ=255)

                                                             ggACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAAc                          >  2:2744705/1‑93 (MQ=255)

                                                             cgACAATACGGTACATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAAc                          <  1:652391/92‑1 (MQ=255)

                                                              gACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACg                         >  2:2824959/1‑93 (MQ=255)

                                                               aCAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGa                        >  2:3947442/1‑93 (MQ=255)

                                                                cAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGAc                       <  1:3980699/93‑1 (MQ=255)

                                                                cAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGAc                       <  1:2838603/93‑1 (MQ=255)

                                                                 aaTACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGAcc                      <  2:1646844/93‑1 (MQ=255)

                                                                 aaTACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGAcc                      <  1:1732849/93‑1 (MQ=255)

                                                                 aaTACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGAcc                      <  2:4209077/93‑1 (MQ=255)

                                                                  aTACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCg                     >  1:4635531/1‑93 (MQ=255)

                                                                    aCGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGAt                   <  2:3666360/93‑1 (MQ=255)

                                                                    aCGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGAt                   <  2:2235779/93‑1 (MQ=255)

                                                                     cGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATc                  <  2:1515793/93‑1 (MQ=255)

                                                                       gTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATcac                >  1:3521110/1‑93 (MQ=255)

                                                                       gTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATcac                >  2:4152947/1‑93 (MQ=255)

                                                                          aaTCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATcaccac             <  1:334979/93‑1 (MQ=255)

                                                                           aTCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACt            >  2:1057857/1‑93 (MQ=255)

                                                                           aTCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACt            >  2:275663/1‑93 (MQ=255)

                                                                              tgctgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACtttt         >  2:3222585/1‑93 (MQ=255)

                                                                              tgctgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACtttt         <  1:1377108/93‑1 (MQ=255)

                                                                               gctgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACttttt        <  1:2745806/93‑1 (MQ=255)

                                                                               gctgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACttttt        <  1:2544264/93‑1 (MQ=255)

                                                                               gctgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGACGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCTCCACttttt        <  1:1948419/93‑1 (MQ=255)

                                                                                ctgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTg       >  1:3158930/1‑93 (MQ=255)

                                                                                 tgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGc      >  1:186740/1‑93 (MQ=255)

                                                                                  gTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCt     >  1:1572182/1‑93 (MQ=255)

                                                                                   tttGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTg    >  1:4216496/1‑93 (MQ=255)

                                                                                     tGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGAt  <  2:1522423/93‑1 (MQ=255)

                                                                                     tGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGAt  <  2:586987/93‑1 (MQ=255)

                                                                                     tGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGAt  <  2:4160914/93‑1 (MQ=255)

GGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGAT  >  NC_000913/2674979‑2675156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`