BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	283,649	T→C	C409R (TGC→CGC)	yagF →	putative dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	283,649	0	T	C	99.3%	452.8 / NA	146	C409R (TGC→CGC)	yagF	putative dehydratase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (75/70); minor base A (1/0); total (76/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

CATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGC  >  NC_000913/283559‑283738

cATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAgcgc                                                                                         <  1:176536/93‑1 (MQ=255)

 aTCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGcc                                                                                        <  2:610952/93‑1 (MQ=255)

   cTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTg                                                                                      >  1:3864811/1‑93 (MQ=255)

     ggACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTgcg                                                                                    <  2:1838229/93‑1 (MQ=255)

     ggACGCCATGACCGTGACCGGCCAGACCGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTgcg                                                                                    >  2:1683114/1‑93 (MQ=255)

      gACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTgcgc                                                                                   >  1:4134854/1‑93 (MQ=255)

       aCGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGcgcg                                                                                  <  1:2571386/93‑1 (MQ=255)

       aCGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGcgcg                                                                                  >  1:3789582/1‑93 (MQ=255)

         gCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAg                                                                                >  2:190468/1‑93 (MQ=255)

          ccATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGc                                                                               <  1:1907660/93‑1 (MQ=255)

           cATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCa                                                                              >  2:1330843/1‑93 (MQ=255)

            aTGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAg                                                                             >  2:2048412/1‑93 (MQ=255)

            aTGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAg                                                                             <  1:867570/93‑1 (MQ=255)

             tgaccgtgaccgGCCAGACGTTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAgg                                                                            <  2:3183180/93‑1 (MQ=255)

             tgaccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCGGCCAGGGCCTGCGCGGGCAgg                                                                            >  1:3226054/1‑93 (MQ=255)

             tgaccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAgg                                                                            >  1:3277393/1‑93 (MQ=255)

              gcccgggaccgGCCAGACGGTGGGCGAGAACCTGGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGa                                                                           <  1:534657/91‑1 (MQ=255)

               accgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGTGTCCGCGCGCCGGGGGCGCTTCAGCCAGCGGCTGCGCGCGCAGCAc                                                                          >  2:1108722/1‑93 (MQ=255)

               accgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGAc                                                                          >  2:1703931/1‑93 (MQ=255)

                ccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACg                                                                         >  2:2766779/1‑93 (MQ=255)

                ccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACg                                                                         >  1:2561391/1‑93 (MQ=255)

                ccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACg                                                                         <  1:198890/93‑1 (MQ=255)

                 cgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACgg                                                                        >  1:1305254/1‑93 (MQ=255)

                     accgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTa                                                                    <  2:2389600/93‑1 (MQ=255)

                      ccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCCCGAGCAGGACGGCGTag                                                                   >  2:2741047/1‑93 (MQ=255)

                         gCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGc                                                                >  1:3923853/1‑93 (MQ=255)

                         gCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGc                                                                >  2:2392431/1‑93 (MQ=255)

                         gCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGAGCGAGCAGGACGGCGTAGAGc                                                                >  2:446100/1‑93 (MQ=255)

                           cAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCg                                                              >  2:4239840/1‑93 (MQ=255)

                              aCGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGAt                                                           <  2:1870347/93‑1 (MQ=255)

                               cGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGCGCCGGATg                                                          >  1:3334846/1‑93 (MQ=255)

                               cGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATg                                                          <  1:3530224/93‑1 (MQ=255)

                                ggTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGa                                                         >  2:4340235/1‑93 (MQ=255)

                                ggTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGAGCCTGCGCGAGCAGGACGGCGTAGAGCCGGCTGa                                                         >  1:2742200/1‑93 (MQ=255)

                                 gTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAc                                                        >  2:4622296/1‑93 (MQ=255)

                                 gTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAc                                                        <  2:376506/93‑1 (MQ=255)

                                  tGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACg                                                       >  2:4436185/1‑93 (MQ=255)

                                   gggCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCTCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGt                                                      <  1:3307356/93‑1 (MQ=255)

                                   gggCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGt                                                      <  2:4549792/93‑1 (MQ=255)

                                    ggCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTg                                                     <  2:2520292/93‑1 (MQ=255)

                                     gCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGa                                                    <  2:3712805/93‑1 (MQ=255)

                                       gagaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATc                                                  >  1:1098175/1‑93 (MQ=255)

                                        agaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATcc                                                 >  1:2257990/1‑93 (MQ=255)

                                        agaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATcc                                                 <  2:3457074/93‑1 (MQ=255)

                                         gaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCt                                                >  2:4140675/1‑93 (MQ=255)

                                         gaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCt                                                <  2:2500880/93‑1 (MQ=255)

                                          aaCCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTg                                               >  2:1549698/1‑93 (MQ=255)

                                             cTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgccg                                            >  2:3098298/1‑93 (MQ=255)

                                              ttGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgccgc                                           >  1:3968239/1‑93 (MQ=255)

                                              ttGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgccgc                                           >  1:1539598/1‑93 (MQ=255)

                                                gAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGccgccg                                         <  1:4326948/93‑1 (MQ=255)

                                                gAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGccgccg                                         >  2:2159128/1‑93 (MQ=255)

                                                  aTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTGCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGga                                       <  2:1417752/93‑1 (MQ=255)

                                                   tggtggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGgag                                      >  2:4284426/1‑93 (MQ=255)

                                                   tggtggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGgag                                      >  2:401081/1‑93 (MQ=255)

                                                    ggtggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGgcga                                     >  2:266935/1‑93 (MQ=255)

                                                     gtggCAGTCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGATCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGaa                                    <  1:526765/93‑1 (MQ=255)

                                                     gtggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGaa                                    <  1:3809480/93‑1 (MQ=255)

                                                      tggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGCAGGCGTAGAGCCCGATGACGTGATTCTGCCGCCGGAGAAg                                   >  2:1610113/1‑93 (MQ=255)

                                                      tggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGACGACGTGATCCTGCAGCCGGAGAAg                                   >  2:2843998/1‑93 (MQ=255)

                                                        gCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGc                                 >  2:2110302/1‑93 (MQ=255)

                                                        gCAGGCGGCCGATCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGc                                 <  1:1205564/93‑1 (MQ=255)

                                                          aGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaa                               >  1:2657587/1‑93 (MQ=255)

                                                          aGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaa                               >  1:3871797/1‑93 (MQ=255)

                                                           ggCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaaa                              >  1:37668/1‑93 (MQ=255)

                                                           ggCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaaa                              >  1:1484486/1‑93 (MQ=255)

                                                              gTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGc                           <  2:2654062/93‑1 (MQ=255)

                                                              gTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGc                           <  1:1517393/93‑1 (MQ=255)

                                                               tCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCg                          >  2:622539/1‑93 (MQ=255)

                                                               tCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCg                          >  2:3686688/1‑93 (MQ=255)

                                                               tCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCg                          >  1:4475208/1‑93 (MQ=255)

                                                                ccGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGa                         <  1:996147/93‑1 (MQ=255)

                                                                 cGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaa                        >  1:2396880/1‑93 (MQ=255)

                                                                 cGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaa                        >  2:1764718/1‑93 (MQ=255)

                                                                  gAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaaa                       <  1:2345388/93‑1 (MQ=255)

                                                                  gAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaaa                       <  1:1559667/93‑1 (MQ=255)

                                                                  gAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaaa                       <  1:2765988/93‑1 (MQ=255)

                                                                  gAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaaa                       >  1:691765/1‑93 (MQ=255)

                                                                   aGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCACAAGCGAAAg                      >  1:1129756/1‑93 (MQ=255)

                                                                   aGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAg                      <  1:2888625/93‑1 (MQ=255)

                                                                   aGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAg                      <  1:772222/93‑1 (MQ=255)

                                                                    gcgcGGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAgg                     <  2:1318263/93‑1 (MQ=255)

                                                                    gcgcCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAGAgg                     >  1:3270662/1‑93 (MQ=255)

                                                                          ggCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGAc               <  1:2178991/93‑1 (MQ=255)

                                                                          ggCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGAc               <  1:903219/93‑1 (MQ=255)

                                                                           gcgcgcTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGAcc              <  2:378197/93‑1 (MQ=255)

                                                                             gcgcTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTc            >  2:3953741/1‑93 (MQ=255)

                                                                             gcgcTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTc            <  1:4453471/93‑1 (MQ=255)

                                                                               gcTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGa          >  1:291480/1‑93 (MQ=255)

                                                                                gttCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGAc         <  1:1313281/92‑1 (MQ=255)

                                                                                cTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGAc         >  1:1107797/1‑93 (MQ=255)

                                                                                cTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGAc         <  1:1680859/93‑1 (MQ=255)

                                                                                   ccGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGt      <  2:1050115/93‑1 (MQ=255)

                                                                                    cGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTc     <  1:908438/93‑1 (MQ=255)

                                                                                    cGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTc     >  1:3136192/1‑93 (MQ=255)

                                                                                    cGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTc     <  1:1388492/93‑1 (MQ=255)

                                                                                      ccAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTg   >  1:1546490/1‑93 (MQ=255)

                                                                                       cAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGc  <  1:4063528/93‑1 (MQ=255)

                                                                                       cAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGc  <  1:2382997/93‑1 (MQ=255)

                                                                                       cAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGc  <  2:704984/93‑1 (MQ=255)

CATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGC  >  NC_000913/283559‑283738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`