BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,729,464	T→C	intergenic (‑286/+156)	rrsG ← / ← clpB	16S ribosomal RNA/ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,729,464	0	T	C	99.4%	478.9 / NA	159	intergenic (‑286/+156)	rrsG/clpB	16S ribosomal RNA/ClpB
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (68/90); minor base A (0/1); total (68/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 164 total aligned reads displayed.

GCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACAT  >  NC_000913/2729374‑2729553

ccTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCa                                                                                         <  1:1811645/92‑1 (MQ=255)

 cTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGACCCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAg                                                                                        <  1:4169857/93‑1 (MQ=255)

 cTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAg                                                                                        <  2:528468/93‑1 (MQ=255)

   aCAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAgtg                                                                                      <  1:1594270/93‑1 (MQ=255)

    cAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAgtgg                                                                                     <  2:2582893/93‑1 (MQ=255)

     agagTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAgtggt                                                                                    <  2:2112665/93‑1 (MQ=255)

      gagTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAgtggtg                                                                                   <  1:1844153/93‑1 (MQ=255)

      gagTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAgtggtg                                                                                   >  1:628460/1‑93 (MQ=255)

      gagTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGACAgtggtg                                                                                   <  2:4581433/93‑1 (MQ=255)

      gagTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGACAgtggtg                                                                                   >  2:3601398/1‑93 (MQ=255)

       agTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGtggtgg                                                                                  <  2:3942865/93‑1 (MQ=255)

       agTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGtggtgg                                                                                  >  1:2293079/1‑93 (MQ=255)

          cAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGgcgc                                                                               >  2:1466378/1‑93 (MQ=255)

            aGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCAt                                                                             >  2:3615974/1‑93 (MQ=255)

             gCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCAtt                                                                            <  2:2535077/93‑1 (MQ=255)

             gCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCAtt                                                                            >  2:1535865/1‑93 (MQ=255)

              cATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATta                                                                           <  2:4408305/93‑1 (MQ=255)

              cATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATta                                                                           >  1:2454726/1‑93 (MQ=255)

               aTTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATtat                                                                          >  2:1992745/1‑93 (MQ=255)

               aTTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATtat                                                                          >  2:2362448/1‑93 (MQ=255)

               aTTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATtat                                                                          >  1:1408099/1‑93 (MQ=255)

                tttCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATtata                                                                         >  1:2367118/1‑93 (MQ=255)

                  tCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAgg                                                                       >  1:1517991/1‑93 (MQ=255)

                  tCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACACCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAgg                                                                       >  2:1029023/1‑93 (MQ=255)

                    tttGCTTTTCTCTGTTGAGATTCTCAGGAGACCCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGa                                                                     <  2:3117572/93‑1 (MQ=255)

                    tttGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGa                                                                     >  1:2209668/1‑93 (MQ=255)

                    tttGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGa                                                                     >  2:3871270/1‑93 (MQ=255)

                       gCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAggt                                                                  >  2:3264034/1‑91 (MQ=255)

                       gCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGtt                                                                  >  1:4043163/1‑93 (MQ=255)

                       gCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGGGTTTGCCGTTGTTCCGTGCCAGTGGGGGGGCATTTTAGGGGGtt                                                                  >  2:4205467/1‑93 (MQ=17)

                       gCTTTCCTCTTTGAAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGtt                                                                  <  2:2352408/93‑1 (MQ=14)

                        cTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTa                                                                 <  1:1352087/93‑1 (MQ=255)

                         ttttCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTAt                                                                >  2:4558535/1‑93 (MQ=255)

                         ttttCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTAt                                                                <  2:1743153/93‑1 (MQ=255)

                          tttCTCTGTTGAGATTCTCAGGAGACCCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTAtt                                                               <  2:3271734/93‑1 (MQ=255)

                          tttCTCTGTTGAGATTCTCAGGAGACCCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTAtt                                                               <  2:3970847/93‑1 (MQ=255)

                           ttttctGTGGAGATTCTCAGGAGACCCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTc                                                              <  1:1533342/90‑1 (MQ=14)

                            tctcTTTTGAGATTCTCAGGAGACCCCCGCCGCCCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTcc                                                             <  2:4070285/93‑1 (MQ=14)

                            tctcTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTcc                                                             <  2:2140488/93‑1 (MQ=255)

                            tctcTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTcc                                                             <  2:1577872/93‑1 (MQ=255)

                             ctctGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCg                                                            >  2:2980959/1‑93 (MQ=255)

                              tctGTTGAGATTCTCAGGAGACCCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCgg                                                           <  2:4456835/93‑1 (MQ=255)

                              tctGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCgg                                                           >  1:3016977/1‑93 (MQ=255)

                               ctGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGc                                                          <  2:1092777/93‑1 (MQ=255)

                               ctGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGc                                                          >  1:3343253/1‑93 (MQ=255)

                                tGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGGTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGcc                                                         >  2:3952053/1‑93 (MQ=255)

                                 gTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCt                                                        >  2:4162160/1‑93 (MQ=255)

                                 gTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCt                                                        <  2:2081347/93‑1 (MQ=255)

                                   tGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGa                                                      <  1:964193/93‑1 (MQ=255)

                                   tGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGa                                                      >  1:4642460/1‑93 (MQ=255)

                                   tGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGa                                                      <  1:1973731/93‑1 (MQ=255)

                                    gagaTTCTCAGGAGACCCCCTCCGACCCGGCGGCGTGGTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGAc                                                     <  1:1968515/93‑1 (MQ=14)

                                      gaTTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACaa                                                   <  2:3854921/93‑1 (MQ=25)

                                      gaTTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACaa                                                   <  2:799890/93‑1 (MQ=25)

                                      gaTTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACaa                                                   <  2:1069417/93‑1 (MQ=25)

                                      gaTTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACaa                                                   <  1:885677/93‑1 (MQ=25)

                                        ttCTCAGGAGACCCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGc                                                 <  2:3241931/93‑1 (MQ=255)

                                          ctcAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGa                                               >  1:4236875/1‑93 (MQ=255)

                                           tcAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGaa                                              >  1:1105687/1‑93 (MQ=255)

                                           tcAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGaa                                              <  1:776474/93‑1 (MQ=255)

                                           tcAGGAGAACCCCGCCGACCCGGCGGCGGGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGaa                                              >  2:4238718/1‑93 (MQ=255)

                                            cAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGaaa                                             >  1:191783/1‑93 (MQ=255)

                                              ggAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGaaaaa                                           >  1:3318769/1‑93 (MQ=255)

                                              ggAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGaaaaa                                           >  2:2264069/1‑93 (MQ=255)

                                                  acccccGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAAtata                                       <  2:556713/91‑1 (MQ=255)

                                                    ccccGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATaaa                                     <  1:2159669/93‑1 (MQ=255)

                                                      ccgccgACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATaaaaa                                   <  1:266872/93‑1 (MQ=255)

                                                      ccgccgACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATaaaaa                                   <  2:1812985/93‑1 (MQ=255)

                                                      ccgccgACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATaaaaa                                   <  1:430695/93‑1 (MQ=255)

                                                       cgccgACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAAc                                  >  2:1965276/1‑93 (MQ=255)

                                                          cgACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACttt                               <  1:67352/93‑1 (MQ=255)

                                                           gACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTa                              <  2:2891694/93‑1 (MQ=255)

                                                           gACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTa                              <  1:1825896/93‑1 (MQ=255)

                                                           gACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTa                              <  1:3439003/93‑1 (MQ=255)

                                                           gACCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTa                              <  2:385552/93‑1 (MQ=255)

                                                            aCCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTAt                             >  2:3447439/1‑93 (MQ=255)

                                                            aCCCGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTAt                             >  2:3571105/1‑93 (MQ=255)

                                                             cccGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATc                            <  1:1568641/93‑1 (MQ=255)

                                                             cccGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATc                            <  2:2788303/93‑1 (MQ=255)

                                                              ccGGCGGCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCg                           <  1:517168/93‑1 (MQ=255)

                                                               cggcggCGTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGt                          <  1:1879955/93‑1 (MQ=255)

                                                                 gcggcgTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTc                        <  2:4321410/93‑1 (MQ=255)

                                                                  cggcgTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCCGACAAGCGAAAAATATAAAAACTTTatcgatcg                       >  2:2502801/1‑93 (MQ=255)

                                                                    gcgTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCt                     <  2:2939232/93‑1 (MQ=255)

                                                                    gcgTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCt                     >  2:549560/1‑93 (MQ=255)

                                                                     cgTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTc                    <  2:3302825/93‑1 (MQ=255)

                                                                     cgTGTTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTc                    >  1:2899819/1‑93 (MQ=255)

                                                                       tgtTTGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCAc                  >  1:2207565/1‑93 (MQ=255)

                                                                        gtTTGCTGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACt                 <  2:3606432/93‑1 (MQ=255)

                                                                         tttGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACtt                >  2:428442/1‑93 (MQ=255)

                                                                         tttGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACtt                <  2:634038/93‑1 (MQ=255)

                                                                          ttGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACttt               <  2:4652598/93‑1 (MQ=255)

                                                                          ttGCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACttt               >  1:255005/1‑93 (MQ=255)

                                                                            gCCGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTc             >  2:496654/1‑93 (MQ=255)

                                                                             ccGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCa            <  1:1113278/93‑1 (MQ=255)

                                                                              cGTTGTTCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAg           >  2:1494072/1‑93 (MQ=255)

                                                                               gttgttCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAgg          >  1:2656301/1‑93 (MQ=255)

                                                                                  gttCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTTAGGCaa       >  1:3716944/1‑93 (MQ=255)

                                                                                    tCCGTGCCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCaaaa     <  1:3247189/93‑1 (MQ=255)

                                                                                       gtgcCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACAt  >  1:4521175/1‑93 (MQ=255)

GCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACAT  >  NC_000913/2729374‑2729553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`