BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	285,775	A→G	Y386C (TAC→TGC)	yagG →	putative permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	285,775	0	A	G	99.2%	378.0 / NA	125	Y386C (TAC→TGC)	yagG	putative permease
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (66/58); minor base T (0/1); total (66/59)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 136 total aligned reads displayed.

CCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGCTC  >  NC_000913/285686‑285863

    ggcGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAAc                                                                                   <  2:2491013/91‑1 (MQ=255)

    gACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAAc                                                                                   <  1:2164563/93‑1 (MQ=255)

     aCGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACt                                                                                  <  2:3493199/93‑1 (MQ=255)

      cGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCTGTGGTGGGCTGGATCCTGGCGTTCGTCAACTa                                                                                 <  2:3923086/93‑1 (MQ=255)

      cGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTa                                                                                 <  1:2113822/93‑1 (MQ=255)

      cGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTa                                                                                 <  1:3869671/93‑1 (MQ=255)

      cGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTa                                                                                 >  1:182836/1‑93 (MQ=255)

       gggCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTAt                                                                                <  1:1971642/93‑1 (MQ=255)

         gCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGTGTGGATCCTGGCGTGCGTCAACTATTc                                                                              >  1:1633820/1‑93 (MQ=255)

         gCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTc                                                                              <  2:3726346/93‑1 (MQ=255)

         gCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTc                                                                              <  1:3734800/93‑1 (MQ=255)

          cTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTAtttc                                                                             >  2:3447568/1‑91 (MQ=255)

          cTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTcc                                                                             <  2:1345347/93‑1 (MQ=255)

          cTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTcc                                                                             >  1:405105/1‑93 (MQ=255)

          cTGGTGTCCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTcc                                                                             <  2:1791081/93‑1 (MQ=255)

             gtgtTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGcc                                                                          <  1:1841740/93‑1 (MQ=255)

              tgtTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCca                                                                         >  1:4418547/1‑93 (MQ=255)

                ttCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagc                                                                       <  1:3391915/93‑1 (MQ=255)

                   tcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagc                                                                    >  2:2627039/1‑93 (MQ=255)

                   tcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagc                                                                    >  1:1620776/1‑93 (MQ=255)

                   tcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCGGGCGTGCGTCAACTATTCCGCcagcaga                                                                    >  2:441129/1‑92 (MQ=255)

                     cACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagcag                                                                  <  1:3974761/93‑1 (MQ=255)

                     cACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagcag                                                                  >  2:2574383/1‑93 (MQ=255)

                     cACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagcag                                                                  >  1:493521/1‑93 (MQ=255)

                      acctacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCagcagcagc                                                                 >  1:2855448/1‑93 (MQ=255)

                       cctacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCg                                                                >  1:3856766/1‑93 (MQ=255)

                       cctacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGGGCGTCAACTATTCCGCCAGCAGCAGCg                                                                >  1:3287172/1‑93 (MQ=255)

                          acctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGGAGCAGCGGGc                                                             >  2:1488110/1‑93 (MQ=255)

                           cctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCa                                                            <  1:1460575/93‑1 (MQ=255)

                            ctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAg                                                           >  2:1071777/1‑93 (MQ=255)

                            ctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGGGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAg                                                           >  1:2984813/1‑93 (MQ=255)

                             tGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATTCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGc                                                          >  1:1151115/1‑93 (MQ=255)

                             tGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGc                                                          <  1:2829529/93‑1 (MQ=255)

                             tGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGc                                                          >  1:1702832/1‑93 (MQ=255)

                                tCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCgg                                                       <  2:4063769/93‑1 (MQ=255)

                                  aGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGtt                                                     >  1:1989839/1‑93 (MQ=255)

                                   gCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTg                                                    >  2:3054938/1‑93 (MQ=255)

                                    ccTGAAGATTGGCCTGGCGATTGTCCGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTGAACTATTCCGCCAGCAGCAGCGTTCAGCCGGTTGa                                                   <  2:4398624/93‑1 (MQ=255)

                                     cTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGGTCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAg                                                  >  2:2972481/1‑93 (MQ=255)

                                     cTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTCTTCCGCCAGCAGCAGCGTGCAGCCGGTTGAg                                                  >  1:1950017/1‑93 (MQ=255)

                                     cTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAg                                                  >  1:1380567/1‑93 (MQ=255)

                                     cTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAg                                                  >  2:4470859/1‑93 (MQ=255)

                                     cTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAg                                                  <  1:4477467/93‑1 (MQ=255)

                                     cTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAg                                                  >  1:2979557/1‑93 (MQ=255)

                                     cTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGGTGAg                                                  >  1:3719949/1‑93 (MQ=255)

                                      tGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAgg                                                 >  2:199943/1‑93 (MQ=255)

                                      tGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAgg                                                 <  1:1100036/93‑1 (MQ=255)

                                       gAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAggg                                                >  1:1767017/1‑92 (MQ=255)

                                       gAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGt                                                <  1:1147826/93‑1 (MQ=255)

                                         aGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGc                                              >  2:197445/1‑93 (MQ=255)

                                         aGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGc                                              <  2:220862/93‑1 (MQ=255)

                                         aGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGc                                              >  2:3107995/1‑93 (MQ=255)

                                         aGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGGTGAGGTGc                                              >  2:3234139/1‑93 (MQ=255)

                                           aTTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTc                                            <  2:1683550/93‑1 (MQ=255)

                                           aTTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTc                                            >  2:856947/1‑93 (MQ=255)

                                           aTTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTc                                            <  1:687126/93‑1 (MQ=255)

                                           aTTGGCCTGGCGATTGGCGGGGCGGTGGTCGGCTGGGTCCTGGGGTGCGTCAACTATTCCGCCAGCCGAAGCCAGCAGCCGGTTGAGGTGCta                                            >  2:3706691/1‑92 (MQ=255)

                                            ttGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTca                                           <  2:428852/93‑1 (MQ=255)

                                             tGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTcac                                          <  2:2795124/93‑1 (MQ=255)

                                               gCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTcacca                                        >  1:4320556/1‑93 (MQ=255)

                                                 cTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCaccacc                                      >  1:2970066/1‑93 (MQ=255)

                                                  tGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCAccacca                                     <  2:4685934/93‑1 (MQ=255)

                                                  tGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGGGCTCAccacca                                     >  2:50667/1‑93 (MQ=255)

                                                   ggCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCAccaccac                                    >  2:4104428/1‑92 (MQ=255)

                                                      gATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCaa                                 >  2:3808234/1‑93 (MQ=255)

                                                       aTTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCaaa                                >  1:1701032/1‑93 (MQ=255)

                                                       aTTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCaaa                                >  1:886237/1‑93 (MQ=255)

                                                        ttGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCaaaa                               <  2:794970/93‑1 (MQ=255)

                                                        ttGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCaaaa                               >  2:2230393/1‑93 (MQ=255)

                                                           gcggggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttc                            <  2:1587304/93‑1 (MQ=255)

                                                             ggggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctg                          <  2:1253133/93‑1 (MQ=255)

                                                               ggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgtt                        >  1:1705359/1‑93 (MQ=255)

                                                               ggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgtt                        <  1:123906/93‑1 (MQ=255)

                                                                gcggTGGTTGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttc                       <  2:1581209/93‑1 (MQ=255)

                                                                gcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttc                       <  1:4478796/93‑1 (MQ=255)

                                                                gcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttc                       <  2:4010623/93‑1 (MQ=255)

                                                                gcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttc                       <  1:2097024/93‑1 (MQ=255)

                                                                   gtggtgGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGc                    >  2:2261097/1‑93 (MQ=255)

                                                                   gtggtgGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGc                    <  2:765010/93‑1 (MQ=255)

                                                                     ggtggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgt                  <  1:4057311/93‑1 (MQ=255)

                                                                       tggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgtgg                >  1:1198036/1‑93 (MQ=255)

                                                                         ggCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgtggtg              >  1:2644896/1‑93 (MQ=255)

                                                                           cTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCCCCATCAAAATTCTGTTCTGCGTGGTGcc            <  1:3181658/93‑1 (MQ=255)

                                                                           cTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGcc            >  2:1949215/1‑93 (MQ=255)

                                                                           cTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGcc            <  2:3423265/93‑1 (MQ=255)

                                                                            tGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCg           >  1:4321562/1‑93 (MQ=255)

                                                                            tGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCg           >  1:4545326/1‑93 (MQ=255)

                                                                              gATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggt         >  1:2201993/1‑93 (MQ=255)

                                                                              gATCATGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggt         <  1:3709220/93‑1 (MQ=255)

                                                                               aTCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtg        >  2:3926781/1‑93 (MQ=255)

                                                                               aTCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtg        >  2:4271638/1‑93 (MQ=255)

                                                                                 ccTGGCGTGCGTCAGCTATTCCGCCAGCAGCAGCGTGCAGCCGGTGGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtggt      <  2:1669324/93‑1 (MQ=255)

                                                                                  cTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGgtggtg     >  1:4635894/1‑93 (MQ=255)

                                                                                   tGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGc    <  2:1112169/93‑1 (MQ=255)

                                                                                   tGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGc    >  2:3687469/1‑93 (MQ=255)

                                                                                   tGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGc    >  1:2462149/1‑93 (MQ=255)

                                                                                    ggCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGct   >  1:2051140/1‑93 (MQ=255)

                                                                                    ggCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGct   >  1:2796993/1‑93 (MQ=255)

                                                                                    ggCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGct   >  2:1374128/1‑93 (MQ=255)

                                                                                     gcgtgcgtCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGctc  <  1:73029/93‑1 (MQ=255)

CCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGCTC  >  NC_000913/285686‑285863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`