BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,754,691	T→C	V171A (GTT→GCT)	intA →	prophage CP4‑57 integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,754,691	0	T	C	100.0%	559.6 / NA	177	V171A (GTT→GCT)	intA	prophage CP4‑57 integrase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (85/92); total (85/92)

Only 100 of 180 total aligned reads displayed.

CAATCGGTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGTTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGG  >  NC_000913/2754601‑2754778

   tCGGTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGGTCAGGCCAGAGGTGCATTAGAGACTGCTcgcc                                                                                    >  1:2756998/1‑93 (MQ=255)

     ggTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTcgccgc                                                                                  >  1:4026745/1‑93 (MQ=255)

      gTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCgccgcc                                                                                 <  1:3557740/93‑1 (MQ=255)

      gTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCgccgcc                                                                                 >  2:3038416/1‑93 (MQ=255)

      gTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCgccgcc                                                                                 >  1:249110/1‑93 (MQ=255)

       tGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCt                                                                                <  2:2987175/93‑1 (MQ=255)

       tGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCt                                                                                <  1:15550/93‑1 (MQ=255)

         atatCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCttt                                                                              >  2:2453798/1‑93 (MQ=255)

          tatCAGTATCACTGAGATTAAGGCTCATTCTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTg                                                                             <  2:3122516/93‑1 (MQ=255)

          tatCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTg                                                                             <  2:4424803/93‑1 (MQ=255)

           atCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGt                                                                            <  2:1060797/93‑1 (MQ=255)

            tCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTc                                                                           >  1:7485/1‑93 (MQ=255)

            tCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTc                                                                           >  1:799559/1‑93 (MQ=255)

            tCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTc                                                                           <  1:3411228/93‑1 (MQ=255)

            tCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTc                                                                           <  1:1817298/93‑1 (MQ=255)

             cAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCa                                                                          <  1:1309655/93‑1 (MQ=255)

              aGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAg                                                                         >  1:2069645/1‑93 (MQ=255)

              aGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAg                                                                         >  1:4547535/1‑93 (MQ=255)

              aGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAg                                                                         >  2:1212011/1‑93 (MQ=255)

                 aTCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGt                                                                      <  2:445826/93‑1 (MQ=255)

                  tCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTa                                                                     <  2:1000131/93‑1 (MQ=255)

                  tCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTa                                                                     >  2:1909070/1‑93 (MQ=255)

                   cACTGAGATTAAGGCTCATTCTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTAt                                                                    <  2:4122921/93‑1 (MQ=255)

                    aCTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTAtt                                                                   >  1:2177258/1‑93 (MQ=255)

                    aCTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTAtt                                                                   >  2:3073953/1‑93 (MQ=255)

                    aCTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTAtt                                                                   >  1:1434290/1‑93 (MQ=255)

                      tGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGCCTGCTCGCCGCCTTTGTCAGCGTATTaa                                                                 <  2:3509609/93‑1 (MQ=255)

                       gagaTTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAAc                                                                >  1:3666890/1‑93 (MQ=255)

                       gagaTTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAAc                                                                >  1:4648734/1‑93 (MQ=255)

                       gagaTTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAAc                                                                <  2:3346877/93‑1 (MQ=255)

                         gaTTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGa                                                              >  2:1526115/1‑93 (MQ=255)

                          aTTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGaa                                                             <  2:2201711/93‑1 (MQ=255)

                          aTTAAGGCTCATACTCTCATTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGCGCATTAGAGTCTGCTCGCCGCCTTTGTCAGCGTATTAACGaa                                                             >  2:566455/1‑93 (MQ=255)

                           ttAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAg                                                            <  2:2215206/93‑1 (MQ=255)

                            tAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGt                                                           >  2:555403/1‑93 (MQ=255)

                            tAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGt                                                           <  2:1752014/93‑1 (MQ=255)

                             aaGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTc                                                          <  1:1061513/93‑1 (MQ=255)

                              aGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCa                                                         >  2:2920007/1‑93 (MQ=255)

                              aGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCa                                                         <  2:1184093/93‑1 (MQ=255)

                              aGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCa                                                         <  1:4273526/93‑1 (MQ=255)

                               ggCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCAt                                                        >  1:1387639/1‑93 (MQ=255)

                               ggCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGACAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCAt                                                        >  1:1740978/1‑93 (MQ=255)

                                 cTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGa                                                      <  2:1422297/93‑1 (MQ=255)

                                  tCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGAt                                                     <  2:4292184/93‑1 (MQ=255)

                                  tCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGAt                                                     <  2:4419197/93‑1 (MQ=255)

                                   cATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGAtt                                                    >  1:994659/1‑93 (MQ=255)

                                   cATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGAtt                                                    <  1:2269204/93‑1 (MQ=255)

                                   cATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGAtt                                                    >  1:4170523/1‑93 (MQ=255)

                                    aTACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGACGTCATGAttt                                                   >  2:4106830/1‑93 (MQ=255)

                                     tACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTCACGAAGTCATGATTTa                                                  >  1:3681962/1‑93 (MQ=255)

                                     tACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTa                                                  >  1:1486279/1‑93 (MQ=255)

                                      aCTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTAt                                                 <  1:2560231/93‑1 (MQ=255)

                                      aCTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTAt                                                 <  2:2661415/93‑1 (MQ=255)

                                       ctctGGCTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATg                                                >  2:2908664/1‑93 (MQ=255)

                                         ctGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATgcg                                              <  2:4045529/93‑1 (MQ=255)

                                              tAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGaa                                         <  2:64524/93‑1 (MQ=255)

                                                  gCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAAcaca                                     <  1:2720322/93‑1 (MQ=255)

                                                  gCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAAcaca                                     <  1:1999027/93‑1 (MQ=255)

                                                   cAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAg                                    <  1:1825734/93‑1 (MQ=255)

                                                    aGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAgg                                   >  1:3267612/1‑93 (MQ=255)

                                                    aGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAgg                                   >  1:3844064/1‑93 (MQ=255)

                                                     gTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGc                                  <  2:1099134/93‑1 (MQ=255)

                                                      ttCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACCGGcc                                 <  1:3697445/93‑1 (MQ=255)

                                                      ttCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGcc                                 <  1:3111656/93‑1 (MQ=255)

                                                      ttCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGcc                                 <  1:93914/93‑1 (MQ=255)

                                                         aGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtga                              <  1:3718449/93‑1 (MQ=255)

                                                          gCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgat                             >  1:2610524/1‑93 (MQ=255)

                                                          gCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgat                             <  2:492207/93‑1 (MQ=255)

                                                           ccGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgatt                            <  2:1492681/93‑1 (MQ=255)

                                                            cGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgattg                           >  1:4200553/1‑93 (MQ=255)

                                                             ggTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgattga                          >  1:507937/1‑93 (MQ=255)

                                                             ggTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgattga                          >  1:2149837/1‑93 (MQ=255)

                                                              gTTGAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGTCCtcattgat                         <  2:3927806/93‑1 (MQ=255)

                                                              gTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgattgat                         >  2:2466740/1‑93 (MQ=255)

                                                              gTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgattgat                         <  1:2926048/93‑1 (MQ=255)

                                                                tCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGc                       <  1:3980435/93‑1 (MQ=255)

                                                                tCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGc                       >  2:4537781/1‑93 (MQ=255)

                                                                   ggCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGt                    >  1:1036122/1‑93 (MQ=255)

                                                                   ggCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGt                    <  1:4527386/93‑1 (MQ=255)

                                                                      cAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTcc                 >  1:692075/1‑93 (MQ=255)

                                                                       agagGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCt                >  2:3202790/1‑93 (MQ=255)

                                                                       agagGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCt                >  2:2852329/1‑93 (MQ=255)

                                                                        gagGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTa               >  1:2335391/1‑93 (MQ=255)

                                                                         agGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAg              <  2:2096567/93‑1 (MQ=255)

                                                                          ggTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAgt             <  2:2630152/93‑1 (MQ=255)

                                                                          ggTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAgt             <  1:1181913/93‑1 (MQ=255)

                                                                           gTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAgtg            <  2:779130/93‑1 (MQ=255)

                                                                            tGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAgtgt           <  2:2168770/93‑1 (MQ=255)

                                                                             gCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTTTa          <  2:4313642/93‑1 (MQ=255)

                                                                             gCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTa          <  1:3129607/93‑1 (MQ=255)

                                                                             gCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTa          >  1:63822/1‑93 (MQ=255)

                                                                               aTTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTaaa        <  1:3180470/93‑1 (MQ=255)

                                                                               aTTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTaaa        >  2:1585535/1‑93 (MQ=255)

                                                                                ttAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAat       >  2:23684/1‑93 (MQ=255)

                                                                                ttAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAat       <  2:671017/93‑1 (MQ=255)

                                                                                 tAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAata      <  2:3812726/93‑1 (MQ=255)

                                                                                  agagACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAatat     >  2:1253102/1‑93 (MQ=255)

                                                                                   gagaCTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATc    <  1:1431382/93‑1 (MQ=255)

                                                                                   gagaCTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATc    <  1:4674038/93‑1 (MQ=255)

                                                                                     gaCTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCgg  >  2:2284303/1‑93 (MQ=255)

CAATCGGTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGTTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGG  >  NC_000913/2754601‑2754778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`