BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,805,798	A→G	N216D (AAC→GAC)	proX →	high‑affinity transport system for glycine betaine and proline

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,805,798	0	A	G	100.0%	527.5 / NA	166	N216D (AAC→GAC)	proX	high‑affinity transport system for glycine betaine and proline
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (97/69); total (97/69)

Only 100 of 171 total aligned reads displayed.

AACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCG  >  NC_000913/2805708‑2805887

aaCTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGAc                                                                                         <  1:4618588/93‑1 (MQ=255)

  cTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGa                                                                                       >  1:3855058/1‑93 (MQ=255)

  cTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGa                                                                                       >  1:4154963/1‑93 (MQ=255)

   tACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGaa                                                                                      >  2:815017/1‑93 (MQ=255)

    aCGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAAc                                                                                     <  1:1700322/93‑1 (MQ=255)

      gCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTg                                                                                   >  2:2661002/1‑93 (MQ=255)

        aGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGaa                                                                                 >  2:4071280/1‑93 (MQ=255)

        aGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGaa                                                                                 <  2:3358871/93‑1 (MQ=255)

         gCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAg                                                                                >  2:3125486/1‑93 (MQ=255)

         gCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAg                                                                                >  1:3554341/1‑93 (MQ=255)

         gCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAg                                                                                >  2:2523010/1‑93 (MQ=255)

            atgatgGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCg                                                                             >  1:4553990/1‑93 (MQ=255)

             tgatgGCCGACACCATCAGTCGCTACAAAGAGTGCAAACCGGTGTTTTATTACACCTGGACGCTGTACTGGGTGAGTGACGAACTGAAGCCgg                                                                            <  2:2935191/93‑1 (MQ=255)

             tgatgGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCgg                                                                            >  2:1813182/1‑93 (MQ=255)

             tgatgGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCgg                                                                            >  2:2476233/1‑93 (MQ=255)

               atgGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGc                                                                          >  2:286417/1‑93 (MQ=255)

                 ggCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCaa                                                                        <  2:3972938/93‑1 (MQ=255)

                  gCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCaaa                                                                       >  2:3860524/1‑93 (MQ=255)

                  gCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCaaa                                                                       >  1:1497976/1‑93 (MQ=255)

                    cGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGa                                                                     >  2:3162349/1‑93 (MQ=255)

                       cacCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATgt                                                                  <  1:2575049/93‑1 (MQ=255)

                          cATCAGTCGCTACAAAGAGGGCAAACCGGTTTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATgtcgt                                                               <  2:1470433/93‑1 (MQ=255)

                          cATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATgtcgt                                                               >  2:3684577/1‑93 (MQ=255)

                            tCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCt                                                             <  1:3592264/93‑1 (MQ=255)

                               gTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGt                                                          <  2:108858/93‑1 (MQ=255)

                               gTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGt                                                          <  1:2657225/93‑1 (MQ=255)

                                tCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGtt                                                         >  2:2455319/1‑93 (MQ=255)

                                tCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGtt                                                         >  1:371617/1‑93 (MQ=255)

                                 cGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTg                                                        >  1:4550526/1‑93 (MQ=255)

                                  gCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGc                                                       >  1:1206968/1‑93 (MQ=255)

                                  gCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGc                                                       <  2:3192151/93‑1 (MQ=255)

                                   cTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCa                                                      <  1:2084456/93‑1 (MQ=255)

                                    tACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAg                                                     >  2:216580/1‑93 (MQ=255)

                                     aCAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAgg                                                    >  1:1047105/1‑93 (MQ=255)

                                      cAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGt                                                   <  2:1008605/93‑1 (MQ=255)

                                      cAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGt                                                   >  2:1915990/1‑93 (MQ=255)

                                       aaaGAGGGAAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTg                                                  >  2:4038526/1‑93 (MQ=255)

                                        aaGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGc                                                 <  2:2093323/93‑1 (MQ=255)

                                         agagGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGcc                                                <  2:241173/93‑1 (MQ=255)

                                         agagGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGcc                                                <  1:3008545/93‑1 (MQ=255)

                                         agagGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGcc                                                >  2:2889306/1‑93 (MQ=255)

                                          gagGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCg                                               >  2:3483672/1‑93 (MQ=255)

                                          gagGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCg                                               >  2:2023562/1‑93 (MQ=255)

                                           agGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGt                                              <  1:1649724/93‑1 (MQ=255)

                                            gggCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGtt                                             >  2:3605068/1‑93 (MQ=255)

                                             ggCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTtc                                            <  2:300704/93‑1 (MQ=255)

                                                aaaCCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTcc                                         >  2:3624698/1‑93 (MQ=255)

                                                aaaCCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTcc                                         <  1:329699/93‑1 (MQ=255)

                                                 aaCCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCg                                        >  1:3580526/1‑93 (MQ=255)

                                                  aCCGGCGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGCGCCGTTCTCCGc                                       >  2:1967847/1‑93 (MQ=255)

                                                   ccGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCa                                      >  2:1466427/1‑93 (MQ=255)

                                                    cGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCAc                                     <  2:1543039/93‑1 (MQ=255)

                                                    cGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCAc                                     <  2:1281108/93‑1 (MQ=255)

                                                    cGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCAc                                     <  1:2175291/93‑1 (MQ=255)

                                                    cGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCAc                                     <  1:2754011/93‑1 (MQ=255)

                                                     ggTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACt                                    >  2:2370663/1‑93 (MQ=255)

                                                     ggTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACt                                    <  1:3145910/93‑1 (MQ=255)

                                                      gtgtTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTg                                   >  1:3513870/1‑93 (MQ=255)

                                                      gtgtTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTg                                   >  1:3325592/1‑93 (MQ=255)

                                                       tgtTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGc                                  <  1:3395929/93‑1 (MQ=255)

                                                       tgtTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGc                                  >  1:1555051/1‑93 (MQ=255)

                                                        tttttATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGcc                                 <  1:3656315/92‑1 (MQ=255)

                                                        gtTTTATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGGCGTTCTCCGCACTGcc                                 >  2:4252852/1‑93 (MQ=255)

                                                          tttATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCgg                               <  2:1778265/93‑1 (MQ=255)

                                                          tttATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCgg                               <  1:569319/93‑1 (MQ=255)

                                                          tttATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCgg                               >  1:2321447/1‑93 (MQ=255)

                                                          tttATTACACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCgg                               <  1:3856142/93‑1 (MQ=255)

                                                            tattaCACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGc                             >  1:2203431/1‑93 (MQ=255)

                                                             attaCACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCg                            <  1:1533021/93‑1 (MQ=255)

                                                              ttaCACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGa                           >  2:2624801/1‑93 (MQ=255)

                                                              ttaCACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGa                           >  1:4281266/1‑93 (MQ=255)

                                                              ttaCACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGa                           <  2:2342648/93‑1 (MQ=255)

                                                              ttaCACCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGa                           >  1:4515183/1‑93 (MQ=255)

                                                                acacCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATa                         <  2:2955410/93‑1 (MQ=255)

                                                                 cacCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATaa                        >  2:3856269/1‑93 (MQ=255)

                                                                  acCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATaaa                       >  2:904801/1‑93 (MQ=255)

                                                                  acCTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATaaa                       >  1:3532408/1‑93 (MQ=255)

                                                                    cTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATaaaaa                     <  1:627938/93‑1 (MQ=255)

                                                                    cTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATaaaaa                     <  1:2953508/93‑1 (MQ=255)

                                                                    cTGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATaaaaa                     >  1:1691574/1‑93 (MQ=255)

                                                                     tGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAAc                    <  2:710721/93‑1 (MQ=255)

                                                                     tGGACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAAc                    <  2:656549/93‑1 (MQ=255)

                                                                      ggACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACg                   >  1:2864697/1‑93 (MQ=255)

                                                                       gACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGc                  <  1:4015010/93‑1 (MQ=255)

                                                                       gACGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGc                  <  1:2483031/93‑1 (MQ=255)

                                                                        aCGCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGcc                 <  1:740750/93‑1 (MQ=255)

                                                                          gCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGa               <  1:3537722/93‑1 (MQ=255)

                                                                          gCCGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGCCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGa               >  1:436500/1‑93 (MQ=255)

                                                                            cGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATa             <  2:4649344/93‑1 (MQ=255)

                                                                            cGTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATa             >  1:4160694/1‑93 (MQ=255)

                                                                             gTACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATAc            <  2:45113/93‑1 (MQ=255)

                                                                              tACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATAcc           >  2:3288075/1‑93 (MQ=255)

                                                                              tACTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATAcc           >  2:4281069/1‑93 (MQ=255)

                                                                               aCTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCa          <  1:873006/93‑1 (MQ=255)

                                                                               aCTGGGTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCa          <  2:4539865/93‑1 (MQ=255)

                                                                                  gggTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAAc       <  1:448400/93‑1 (MQ=255)

                                                                                  gggTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAAc       <  2:1600747/93‑1 (MQ=255)

                                                                                   ggTGAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACt      >  1:4323729/1‑93 (MQ=255)

                                                                                      gAGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGcc   <  2:3738616/93‑1 (MQ=255)

                                                                                       aGTGACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCg  <  2:3221732/93‑1 (MQ=255)

AACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCG  >  NC_000913/2805708‑2805887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`