BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,818,582	A→G	N484N (AAT→AAC)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,582	0	A	G	100.0%	639.9 / NA	200	N484N (AAT→AAC)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (100/100); total (100/100)

Only 100 of 206 total aligned reads displayed.

GCCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCATTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGATG  >  NC_000913/2818492‑2818670

gcCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGtt                                                                                        <  1:609449/93‑1 (MQ=255)

gcCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGtt                                                                                        >  1:4206108/1‑93 (MQ=255)

gcCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGtt                                                                                        <  1:3353628/93‑1 (MQ=255)

gcCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGtt                                                                                        >  2:2772378/1‑93 (MQ=255)

gcCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGtt                                                                                        >  2:352693/1‑93 (MQ=255)

gcCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGtt                                                                                        <  1:2495219/93‑1 (MQ=255)

 ccTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTg                                                                                       >  2:1502807/1‑93 (MQ=255)

 ccTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTg                                                                                       >  2:3995400/1‑93 (MQ=255)

  cTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGa                                                                                      <  2:2888482/93‑1 (MQ=255)

   tttCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGAt                                                                                     >  1:4367772/1‑93 (MQ=255)

   tttCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGAt                                                                                     <  2:2338701/93‑1 (MQ=255)

    ttCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATg                                                                                    >  2:1749367/1‑93 (MQ=255)

     tCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATgg                                                                                   <  1:3210170/93‑1 (MQ=255)

        gTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCAt                                                                                >  2:1225144/1‑93 (MQ=255)

        gTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCAt                                                                                <  1:1585256/93‑1 (MQ=255)

        gTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGAAt                                                                                >  1:1351965/1‑93 (MQ=255)

         ttCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATc                                                                               >  1:2386479/1‑93 (MQ=255)

          tCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCa                                                                              <  2:4274211/93‑1 (MQ=255)

          tCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCa                                                                              >  2:3439871/1‑93 (MQ=255)

          tCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCa                                                                              >  1:4022055/1‑93 (MQ=255)

           cGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCaa                                                                             <  2:3672396/93‑1 (MQ=255)

            gCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAAc                                                                            <  1:2355720/93‑1 (MQ=255)

            gCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAAc                                                                            <  2:2460613/93‑1 (MQ=255)

             ccTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAAcc                                                                           <  1:1296289/93‑1 (MQ=255)

               tttATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGc                                                                         >  2:1992981/1‑93 (MQ=255)

                  aTCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCttt                                                                      >  1:532684/1‑93 (MQ=255)

                  aTCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCttt                                                                      >  1:1991972/1‑93 (MQ=255)

                  aTCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTGTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCttt                                                                      <  2:598375/93‑1 (MQ=255)

                   tCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTa                                                                     >  2:1794813/1‑93 (MQ=255)

                   tCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTa                                                                     >  2:1351665/1‑93 (MQ=255)

                   tCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTa                                                                     <  1:840870/93‑1 (MQ=255)

                    cGCCACCCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTAc                                                                    <  2:558302/93‑1 (MQ=255)

                      ccAACGTGATCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCa                                                                  <  2:1122891/93‑1 (MQ=255)

                      ccAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCa                                                                  >  2:3291294/1‑93 (MQ=255)

                      ccAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCa                                                                  <  2:889495/93‑1 (MQ=255)

                       cAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCAt                                                                 <  2:918395/93‑1 (MQ=255)

                        aaCCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATc                                                                >  2:2090367/1‑93 (MQ=255)

                        aaCCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATc                                                                <  2:4559096/93‑1 (MQ=255)

                        aaCCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATc                                                                <  1:664770/93‑1 (MQ=255)

                         aCCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATca                                                               >  2:1212052/1‑93 (MQ=255)

                           cTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATcaac                                                             <  2:3918646/93‑1 (MQ=255)

                            tGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATcaaca                                                            <  2:3235849/93‑1 (MQ=255)

                                cgccgGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCaacaaaca                                                        <  1:4426696/93‑1 (MQ=255)

                                cgccgGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCaacaaaca                                                        <  1:1225144/93‑1 (MQ=255)

                                 gccgGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAg                                                       >  1:2634170/1‑93 (MQ=255)

                                  ccgGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAgc                                                      >  1:128849/1‑93 (MQ=255)

                                     gATTCCGCATATAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCa                                                   >  1:3513430/1‑93 (MQ=255)

                                     gATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATCGCATCAACCGCTTTACCATCAACAAACAGCGCa                                                   >  1:1024614/1‑93 (MQ=255)

                                      aTTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAg                                                  >  1:3373342/1‑93 (MQ=255)

                                       ttCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGt                                                 <  1:583004/93‑1 (MQ=255)

                                          cGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCAc                                              >  2:1813420/1‑93 (MQ=255)

                                          cGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCAc                                              >  1:2660170/1‑93 (MQ=255)

                                           gCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACt                                             >  2:1450080/1‑93 (MQ=255)

                                           gCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACt                                             <  2:228217/93‑1 (MQ=255)

                                           gCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACt                                             >  2:1302502/1‑93 (MQ=255)

                                             aTAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACttt                                           >  1:1939984/1‑93 (MQ=255)

                                             aTAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACttt                                           <  2:2958654/93‑1 (MQ=255)

                                              tAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTg                                          >  2:2467341/1‑93 (MQ=255)

                                                gAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGcc                                        >  1:278782/1‑93 (MQ=255)

                                                 aaTGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCg                                       >  2:1913243/1‑93 (MQ=255)

                                                  aTGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGt                                      >  2:1310450/1‑93 (MQ=255)

                                                    ggCGTTTGACCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTc                                    <  2:1016727/93‑1 (MQ=255)

                                                         ttGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTc                               >  2:415809/1‑93 (MQ=255)

                                                          tGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTcc                              <  2:4369938/93‑1 (MQ=255)

                                                          tGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTcc                              <  1:3549074/93‑1 (MQ=255)

                                                              cgaGCACGCCCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGAt                          <  2:3059707/91‑1 (MQ=255)

                                                              ccAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGAt                          <  2:4512069/93‑1 (MQ=255)

                                                              ccAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGAt                          >  1:181694/1‑93 (MQ=255)

                                                              ccAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGAt                          >  2:4296396/1‑93 (MQ=255)

                                                              ccAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGAt                          <  2:809084/93‑1 (MQ=255)

                                                               cAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATg                         <  1:2987646/93‑1 (MQ=255)

                                                               cAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATg                         >  1:3482094/1‑93 (MQ=255)

                                                                 gCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATggc                       >  1:2171825/1‑92 (MQ=255)

                                                                  cACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTc                      <  2:4432818/93‑1 (MQ=255)

                                                                   aCGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCa                     >  1:1078527/1‑93 (MQ=255)

                                                                   aCGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCa                     >  2:149113/1‑93 (MQ=255)

                                                                   aCGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCa                     <  2:312388/93‑1 (MQ=255)

                                                                    cGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCAt                    >  1:2529651/1‑93 (MQ=255)

                                                                     gACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATa                   >  1:3782409/1‑93 (MQ=255)

                                                                     gACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATa                   >  1:1693819/1‑93 (MQ=255)

                                                                      aCCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAg                  >  1:2932/1‑93 (MQ=255)

                                                                      aCCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAg                  >  1:2149562/1‑93 (MQ=255)

                                                                        cacaGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGcc                >  1:4246346/1‑93 (MQ=255)

                                                                          caGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCtt              <  1:3566009/93‑1 (MQ=255)

                                                                            gCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTa            <  1:3036779/93‑1 (MQ=255)

                                                                            gCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTa            >  1:287556/1‑93 (MQ=255)

                                                                            gCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTa            >  2:2734574/1‑93 (MQ=255)

                                                                             cTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTaa           >  1:901808/1‑93 (MQ=255)

                                                                              ttCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTaaa          <  1:3323714/93‑1 (MQ=255)

                                                                               tCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAAt         >  1:97049/1‑93 (MQ=255)

                                                                               tCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAAt         <  2:17153/93‑1 (MQ=255)

                                                                               tCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAAt         >  1:3982218/1‑93 (MQ=255)

                                                                               tCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAAt         >  2:2678762/1‑93 (MQ=255)

                                                                                 cTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTc       >  2:1699793/1‑93 (MQ=255)

                                                                                 cTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTc       >  2:1539381/1‑93 (MQ=255)

                                                                                  tGGCCTGCTTTGATGGCATCAACCGCTTTACCATCAACAAGCAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCa      <  2:1870678/93‑1 (MQ=255)

                                                                                    gCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGa    <  1:3127119/93‑1 (MQ=255)

                                                                                     ccTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGAt   >  1:44757/1‑93 (MQ=255)

                                                                                      cTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGATg  >  2:4416558/1‑93 (MQ=255)

                                                                                      cTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGATg  >  2:2201168/1‑93 (MQ=255)

GCCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCATTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGATG  >  NC_000913/2818492‑2818670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`