BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,631,832	(T)_6→5	coding (259/1467 nt)	guaB ←	IMP dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,631,827	0	T	.	99.3%	649.2 / ‑2.7	143	coding (264/1467 nt)	guaB	IMP dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (63/79); total (63/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

Only 100 of 164 total aligned reads displayed.

CAAAACCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCA  >  NC_000913/2631736‑2631914

cAAAACCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtt                                                                                        <  1:3660113/93‑1 (MQ=255)

  aaaCCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttt                                                                                      <  1:3881998/93‑1 (MQ=255)

   aaCCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGttttt                                                                                     >  1:1249557/1‑93 (MQ=255)

    aCCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttc                                                                                    <  1:4208745/93‑2 (MQ=255)

    aCCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttc                                                                                    <  1:965213/93‑2 (MQ=255)

      cGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcac                                                                                  <  1:4360919/93‑4 (MQ=255)

      cGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcac                                                                                  <  1:4713986/93‑4 (MQ=255)

      cGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcac                                                                                  >  1:1433021/1‑90 (MQ=255)

      cGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcac                                                                                  <  2:1664169/93‑4 (MQ=255)

       gTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcaca                                                                                 <  2:870137/93‑5 (MQ=255)

       gTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcaca                                                                                 >  2:342754/1‑89 (MQ=255)

       gTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcaca                                                                                 <  1:2694145/93‑5 (MQ=255)

       gTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcaca                                                                                 <  2:1627391/93‑5 (MQ=255)

       gTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGAAGAACAGTCTGCGGTTCAGTCACCACACCAAATTCGTGtttttcaca                                                                                 >  2:1080872/1‑89 (MQ=255)

         tACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACg                                                                              >  2:4293727/1‑93 (MQ=255)

         tACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACg                                                                              <  1:2915136/93‑1 (MQ=255)

          aCGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACgg                                                                             <  1:696375/93‑1 (MQ=255)

            gCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCg                                                                           >  2:73553/1‑93 (MQ=255)

               cGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAAc                                                                        <  2:136923/93‑1 (MQ=255)

                ggTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAAct                                                                       <  2:1032646/93‑1 (MQ=255)

                ggTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAAct                                                                       >  1:4509223/1‑93 (MQ=255)

                ggTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAAct                                                                       <  2:4366138/93‑1 (MQ=255)

                 gTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActt                                                                      <  1:4071183/93‑1 (MQ=255)

                 gTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActt                                                                      >  2:654930/1‑93 (MQ=255)

                 gTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActt                                                                      <  2:4081605/93‑1 (MQ=255)

                  tCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActtc                                                                     <  2:4442187/93‑1 (MQ=255)

                  tCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActtc                                                                     <  1:1679170/93‑1 (MQ=255)

                  tCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActtc                                                                     <  2:1460582/93‑1 (MQ=255)

                  tCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActtc                                                                     <  2:824192/93‑1 (MQ=255)

                   cAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActtct                                                                    <  1:3259319/93‑1 (MQ=255)

                   cAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActtct                                                                    <  2:867196/93‑1 (MQ=255)

                   cAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActtct                                                                    >  1:3990639/1‑93 (MQ=255)

                    aGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActtctt                                                                   <  1:4544317/93‑1 (MQ=255)

                     gTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACttcttc                                                                  >  2:4085937/1‑93 (MQ=255)

                     gTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACAAGATTCGTG‑TTTTTCACACGGCGAACttcttc                                                                  >  1:1757121/1‑93 (MQ=255)

                      ttCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTtcttct                                                                 >  2:370354/1‑93 (MQ=255)

                       tCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTg                                                                <  1:378368/93‑1 (MQ=255)

                           tCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGCCGAACTTCTTCTGCCTg                                                            >  1:1258452/1‑93 (MQ=255)

                            cACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTgg                                                           <  1:2185498/93‑1 (MQ=255)

                              cTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCg                                                         >  1:1040703/1‑93 (MQ=255)

                               ttCGCGGAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGt                                                        >  2:4667428/1‑93 (MQ=255)

                                   cgcAGCGTCGTGGTTGGCAGAACAGTCTGCTGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCaa                                                    <  2:3209363/93‑1 (MQ=255)

                                   cgcAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCaa                                                    <  1:575494/93‑1 (MQ=255)

                                    gcAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAAt                                                   <  1:2407850/93‑1 (MQ=255)

                                    gcAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAAt                                                   <  1:207721/93‑1 (MQ=255)

                                    gcAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAAt                                                   <  1:1786904/93‑1 (MQ=255)

                                    gcAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAAt                                                   <  2:2720035/93‑1 (MQ=255)

                                     cAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATg                                                  >  1:3240905/1‑93 (MQ=255)

                                     cAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATg                                                  <  1:1917033/93‑1 (MQ=255)

                                      aGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATg                                                  <  1:3673283/93‑1 (MQ=255)

                                      aGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATgg                                                 >  2:3316166/1‑93 (MQ=255)

                                      aGCGTCGCGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATgg                                                 >  1:1721333/1‑93 (MQ=255)

                                        cgtcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGAc                                               >  2:902485/1‑93 (MQ=255)

                                         gtcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACa                                              >  1:3796351/1‑93 (MQ=255)

                                         gtcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACa                                              >  2:1521740/1‑93 (MQ=255)

                                          tcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACTGCGAACTTCTTCTGCCTGGCGTTCAATGGACAt                                             >  2:4188070/1‑93 (MQ=255)

                                          tcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAt                                             >  2:3583501/1‑93 (MQ=255)

                                           cgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATg                                            >  2:664359/1‑93 (MQ=255)

                                           cgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATg                                            >  1:237946/1‑93 (MQ=255)

                                           cgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATg                                            <  1:1422385/93‑1 (MQ=255)

                                            gtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGt                                           >  1:1421617/1‑93 (MQ=255)

                                               gtgagCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGtttt                                        <  2:2749478/89‑1 (MQ=255)

                                                   gCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTtgtg                                    <  1:907886/93‑1 (MQ=255)

                                                      gAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGAt                                 <  1:4574716/93‑1 (MQ=255)

                                                      gAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGAt                                 <  2:2464037/93‑1 (MQ=255)

                                                       aaCAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATa                                <  2:3753408/93‑1 (MQ=255)

                                                       aaCAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATa                                >  1:3980750/1‑93 (MQ=255)

                                                         cAGTCTGCGGATCAGTCACCACCCCAGATTCGTG‑TTTTTCACAGGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATaaa                              <  2:2968958/93‑1 (MQ=255)

                                                         cAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATaaa                              <  1:2326164/93‑1 (MQ=255)

                                                            tCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGcc                           <  1:3434609/93‑1 (MQ=255)

                                                            tCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGcc                           <  1:1320682/93‑1 (MQ=255)

                                                             cTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCg                          <  1:1511114/93‑1 (MQ=255)

                                                              tGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGa                         >  2:3917799/1‑93 (MQ=255)

                                                               tccgATCAGTCACCACACCAGATTCGTG‑TTTTTCTCAGTGCAAGCTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGAt                        <  2:3169890/90‑1 (MQ=255)

                                                               gCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGAt                        >  1:3081602/1‑93 (MQ=255)

                                                                cGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATa                       <  2:2151439/93‑1 (MQ=255)

                                                                cGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATa                       <  1:2861947/93‑1 (MQ=255)

                                                                cGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATa                       <  2:2392394/93‑1 (MQ=255)

                                                                  gATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAcc                     <  2:756082/93‑1 (MQ=255)

                                                                   aTCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTCCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCg                    >  1:4021071/1‑93 (MQ=255)

                                                                    tCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGc                   >  2:229873/1‑93 (MQ=255)

                                                                    tCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGc                   >  1:3394431/1‑93 (MQ=255)

                                                                     cAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGcc                  <  1:4290450/93‑1 (MQ=255)

                                                                     cAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGCTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGcc                  >  2:3300730/1‑93 (MQ=255)

                                                                      aGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCt                 <  1:53971/93‑1 (MQ=255)

                                                                      aGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCt                 <  2:2251878/93‑1 (MQ=255)

                                                                       gTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCtt                >  2:2460758/1‑93 (MQ=255)

                                                                       gTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCtt                >  2:1332415/1‑93 (MQ=255)

                                                                         caccacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTcc              <  1:668262/93‑1 (MQ=255)

                                                                          accacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCt             <  2:2137023/93‑1 (MQ=255)

                                                                          accacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCt             <  1:737886/93‑1 (MQ=255)

                                                                            cacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGa           >  2:341151/1‑93 (MQ=255)

                                                                             acaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAg          >  1:1154775/1‑93 (MQ=255)

                                                                               accaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGcc        <  2:2767284/93‑1 (MQ=255)

                                                                               accaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGcc        <  2:4281770/93‑1 (MQ=255)

                                                                                ccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCa       >  2:2020241/1‑93 (MQ=255)

                                                                                 caGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCag      >  1:3909044/1‑93 (MQ=255)

                                                                                    aTTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGc   <  2:784306/93‑1 (MQ=255)

                                                                                    aTTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGc   >  2:2588087/1‑93 (MQ=255)

                                                                                     ttCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCa  >  1:3710171/1‑93 (MQ=255)

CAAAACCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCA  >  NC_000913/2631736‑2631914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`