BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,747,389	Δ1 bp	coding (572/591 nt)	b4540 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,747,388	0	C	.	99.4%	727.6 / ‑2.9	160	coding (571/591 nt)	b4540	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (76/83); minor base T (1/0); total (77/83)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

CTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTG  >  NC_000913/2747296‑2747475

ctTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATc                                                                                         >  2:2392053/1‑93 (MQ=255)

ctTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATc                                                                                         <  1:4663025/93‑1 (MQ=255)

ctTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATc                                                                                         >  1:4585848/1‑93 (MQ=255)

ctTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATc                                                                                         <  1:4438258/93‑1 (MQ=255)

 tttGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATct                                                                                        >  2:1290044/1‑92 (MQ=255)

      gCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaa                                                                                  <  1:834615/93‑1 (MQ=255)

       cAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaa                                                                                 <  1:406151/93‑1 (MQ=255)

        aaaGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCTCAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaaa                                                                                <  2:3792015/93‑1 (MQ=255)

        aaaGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaaa                                                                                >  2:2107223/1‑93 (MQ=255)

         aagaagAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaaaa                                                                               >  1:3247423/1‑93 (MQ=255)

          agaagaGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGTTGTCGGTGCTCGAT‑CTGGAAAAAAt                                                                              >  2:3135243/1‑93 (MQ=255)

          agaagaGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAAt                                                                              <  1:3416476/93‑1 (MQ=255)

            aagaGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGa                                                                            <  2:2365166/93‑1 (MQ=255)

             agagTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGAc                                                                           >  2:82202/1‑93 (MQ=255)

              gagTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGAcc                                                                          <  2:4147083/93‑1 (MQ=255)

               agTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCg                                                                         >  1:4272637/1‑93 (MQ=255)

                gTTTCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGt                                                                        <  2:647755/93‑1 (MQ=255)

                gTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGt                                                                        >  2:4377212/1‑93 (MQ=255)

                gTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGt                                                                        >  2:1844557/1‑93 (MQ=255)

                 ttGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGtt                                                                       <  2:3009599/93‑1 (MQ=255)

                 ttGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGtt                                                                       <  1:1142413/93‑1 (MQ=255)

                    cgcACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTtgat                                                                    <  2:348638/93‑1 (MQ=255)

                     gcACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTtgatg                                                                   >  1:3538765/1‑93 (MQ=255)

                       aCTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAc                                                                 >  1:774087/1‑93 (MQ=255)

                       aCTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAc                                                                 <  2:3685144/93‑1 (MQ=255)

                       aCTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGGGCTCGAT‑CTGGAAAAAATGACCGTtgattaa                                                                 >  1:1062705/1‑90 (MQ=255)

                        cTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAca                                                                >  2:3819661/1‑93 (MQ=255)

                        cTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAca                                                                >  1:2917568/1‑93 (MQ=255)

                         tATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcat                                                               <  1:4370391/93‑1 (MQ=255)

                         tATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcat                                                               >  1:2186916/1‑93 (MQ=255)

                         tATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGCTGCTCGAT‑CTGGAAAAAATGACCGTTGCTGAcat                                                               >  2:1285462/1‑93 (MQ=255)

                          aTCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatc                                                              <  1:4359313/93‑1 (MQ=255)

                          aTCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatc                                                              >  1:383467/1‑93 (MQ=255)

                           tCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatca                                                             <  1:3193968/93‑1 (MQ=255)

                           tCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatca                                                             >  2:3466770/1‑93 (MQ=255)

                            cGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatcat                                                            <  2:2511609/93‑1 (MQ=255)

                             gTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATg                                                           >  1:1887978/1‑93 (MQ=255)

                              tGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATgg                                                          <  1:3379301/93‑1 (MQ=255)

                              tGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATgg                                                          <  2:3151214/93‑1 (MQ=255)

                              tGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATACGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATgg                                                          <  1:2771708/93‑1 (MQ=255)

                                    caTCACGCTCACAAATTTCCCGTCCGAATCAGAATATGCTGCTGTGGGTGCTC‑AAACTGGAACAAATGACCGTTGATGACATCATGGTCCcgc                                                    <  2:2404700/92‑1 (MQ=255)

                                    aaTCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCcgc                                                    >  2:3892166/1‑93 (MQ=255)

                                    aaTCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCcgc                                                    <  1:3438334/93‑1 (MQ=255)

                                     aTCACGCTCACAAATTTCCCGTCGCAATCAGGGTATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCcgcg                                                   >  2:378691/1‑93 (MQ=255)

                                     aTCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCcgcg                                                   >  1:4475199/1‑93 (MQ=255)

                                     aTCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCcgcg                                                   >  1:2480992/1‑93 (MQ=255)

                                       cACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCa                                                 <  1:2918860/93‑1 (MQ=255)

                                         cGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGt                                               <  2:1998524/93‑1 (MQ=255)

                                           cTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGa                                             <  1:504794/93‑1 (MQ=255)

                                             cacaAATTTCCCGTGGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTCCCGCGCAGTGaaa                                           >  2:4032827/1‑93 (MQ=255)

                                              acaAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAAt                                          <  1:4126277/93‑1 (MQ=255)

                                                 aaTTTCCCGTCGCAATCAGGATATGTTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGATCGTTGATGACATCATGGTGCCGCGCAGCGAAATTAt                                       >  2:4218126/1‑93 (MQ=255)

                                                  aTTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATc                                      <  1:3762824/93‑1 (MQ=255)

                                                  aTTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATc                                      >  1:1677252/1‑93 (MQ=255)

                                                  aTTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATc                                      <  2:133431/93‑1 (MQ=255)

                                                  aTTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATc                                      <  1:1741829/93‑1 (MQ=255)

                                                      cccGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTa                                  <  1:3668165/93‑1 (MQ=255)

                                                        cGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTAtt                                <  2:4705901/93‑1 (MQ=255)

                                                          tCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGa                              >  2:531789/1‑93 (MQ=255)

                                                           cGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGat                             >  1:1305156/1‑93 (MQ=255)

                                                           cGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGat                             <  2:3107918/93‑1 (MQ=255)

                                                            gCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGTAGTGAAATTATCGGTATTGata                            <  1:2847308/93‑1 (MQ=255)

                                                            gCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGata                            <  1:2595363/93‑1 (MQ=255)

                                                            gCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGata                            <  2:1941589/93‑1 (MQ=255)

                                                              aaTCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATc                          <  1:1952040/93‑1 (MQ=255)

                                                              aaTCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATc                          <  2:1945870/93‑1 (MQ=255)

                                                               aTCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCa                         <  1:3547630/93‑1 (MQ=255)

                                                               aTCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCa                         >  1:141367/1‑93 (MQ=255)

                                                               aTCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTACCGCGCAGTGAAATTATCGGTATTGATATCa                         >  1:2238103/1‑93 (MQ=255)

                                                               aTCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAAGGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCa                         <  2:1042500/93‑1 (MQ=255)

                                                                tCAGGATATGCTGCTGTCGGTGGTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCaa                        <  2:564114/93‑1 (MQ=255)

                                                                 cAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAAc                       <  1:3979143/93‑1 (MQ=255)

                                                                  atgATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACg                      >  1:2060809/3‑93 (MQ=255)

                                                                   ggATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGa                     <  1:4549289/93‑1 (MQ=255)

                                                                    gATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACgct                    >  1:3322623/1‑91 (MQ=255)

                                                                    gATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGAt                    >  2:2748933/1‑93 (MQ=255)

                                                                    gATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGAt                    <  1:408561/93‑1 (MQ=255)

                                                                    gATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGAt                    >  2:3746380/1‑93 (MQ=255)

                                                                    gATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTAGTGATATCAACGAt                    >  2:2884266/1‑93 (MQ=255)

                                                                     atatGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATg                   <  2:1242454/93‑1 (MQ=255)

                                                                      tatGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGa                  <  2:1608530/93‑1 (MQ=255)

                                                                       atGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGAc                 >  1:189572/1‑93 (MQ=255)

                                                                       atGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGAc                 >  2:1195567/1‑93 (MQ=255)

                                                                        tgctgcTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACt                <  2:744149/93‑1 (MQ=255)

                                                                         gctgctGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTg               <  1:962663/93‑1 (MQ=255)

                                                                         gctgctGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTg               <  2:1821861/93‑1 (MQ=255)

                                                                          ctgctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTgg              >  1:3466045/1‑93 (MQ=255)

                                                                          ctgctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTgg              >  1:2248223/1‑93 (MQ=255)

                                                                           tgctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGa             <  1:3935413/93‑1 (MQ=255)

                                                                              tgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAAt          >  1:3514818/1‑93 (MQ=255)

                                                                              tgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAAt          <  1:318113/93‑1 (MQ=255)

                                                                               gTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCTGCGCAGTGAAATTATCTTAATTTTTATCAACGATGACTGGAAATc         >  1:3616119/1‑93 (MQ=255)

                                                                               gTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATc         <  1:1531778/93‑1 (MQ=255)

                                                                               gTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATc         <  1:670919/93‑1 (MQ=255)

                                                                               gTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATc         <  1:907854/93‑1 (MQ=255)

                                                                                  ggTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGAt      >  1:1871814/1‑93 (MQ=255)

                                                                                  ggTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGAt      <  1:3223290/93‑1 (MQ=255)

                                                                                    tGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTc    <  2:4033476/93‑1 (MQ=255)

                                                                                     gCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCt   <  2:4593328/93‑1 (MQ=255)

                                                                                      cTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTg  >  1:2636935/1‑93 (MQ=255)

CTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTG  >  NC_000913/2747296‑2747475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`