BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,838,055	Δ1 bp	coding (509/1458 nt)	ascF →	PTS system enzyme IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,838,055	0	G	.	98.5%	569.0 / 0.6	130	coding (509/1458 nt)	ascF	PTS system enzyme IIBC
Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (65/62); minor base A (1/1); total (67/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

CAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGC‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGC  >  NC_000913/2837968‑2838142

  aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCaaa                                                                                  >  1:2998620/1‑93 (MQ=255)

  aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCaaa                                                                                  <  2:1764994/93‑1 (MQ=255)

    aGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAtt                                                                                <  1:4342880/93‑1 (MQ=255)

     ggATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAttt                                                                               >  1:1057500/1‑93 (MQ=255)

     ggATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAttt                                                                               >  1:576621/1‑93 (MQ=255)

       aTCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaa                                                                             >  1:935674/1‑93 (MQ=255)

        tCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaaa                                                                            >  2:2890596/1‑93 (MQ=255)

        tCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaaa                                                                            <  2:2822705/93‑1 (MQ=255)

        tCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaaa                                                                            >  2:2432044/1‑93 (MQ=255)

          gCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAAc                                                                          >  1:3846959/1‑93 (MQ=255)

          gCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAAc                                                                          <  1:525738/93‑1 (MQ=255)

           ccGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAAcc                                                                         >  1:1581065/1‑93 (MQ=255)

           ccGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAAcc                                                                         >  1:1184888/1‑93 (MQ=255)

             gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                                                       >  2:4137724/1‑93 (MQ=255)

             gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                                                       <  2:3326029/93‑1 (MQ=255)

             gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                                                       >  2:2508389/1‑93 (MQ=255)

             gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                                                       >  2:2224932/1‑93 (MQ=255)

             gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                                                       <  1:4305649/93‑1 (MQ=255)

              aCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTACAACCAat                                                                      >  2:4672890/1‑93 (MQ=255)

              aCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAat                                                                      <  2:2137040/93‑1 (MQ=255)

               ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAata                                                                     <  1:2661844/93‑1 (MQ=255)

                cTTAACCATTCTGAATGTGATTGGTGCCGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                                    <  1:4357623/93‑1 (MQ=255)

                cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                                    >  2:646978/1‑93 (MQ=255)

                cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                                    <  2:797924/93‑1 (MQ=255)

                cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                                    <  2:3647135/93‑1 (MQ=255)

                 ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                                   >  2:1739962/1‑93 (MQ=255)

                  tAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGt                                                                  >  1:3643110/1‑93 (MQ=255)

                   aaCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTc                                                                 <  1:2303927/93‑1 (MQ=255)

                    aCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCg                                                                <  2:620011/93‑1 (MQ=255)

                    aCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCg                                                                >  1:1076158/1‑93 (MQ=255)

                     ccATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGc                                                               <  1:2406693/93‑1 (MQ=255)

                     ccATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGc                                                               >  1:229542/1‑93 (MQ=255)

                      cATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCt                                                              <  2:589579/93‑1 (MQ=255)

                       aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                                             <  1:681381/93‑1 (MQ=255)

                       aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                                             <  1:1578679/93‑1 (MQ=255)

                         tCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGc                                                           <  1:2001685/93‑1 (MQ=255)

                           tGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGa                                                         >  1:2866219/1‑93 (MQ=255)

                             aaTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAtt                                                       <  2:1210978/93‑1 (MQ=255)

                             aaTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAtt                                                       <  1:2288284/93‑1 (MQ=255)

                             aaTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAtt                                                       <  2:4517299/93‑1 (MQ=255)

                              aTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTg                                                      <  1:3651635/93‑1 (MQ=255)

                                 tgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCa                                                   <  2:1455234/93‑1 (MQ=255)

                                  gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                                  >  2:2556621/1‑93 (MQ=255)

                                  gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                                  >  2:1584270/1‑93 (MQ=255)

                                  gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                                  >  1:825209/1‑93 (MQ=255)

                                   aTTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATc                                                 >  1:4039464/1‑93 (MQ=255)

                                   aTTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATc                                                 <  2:1884240/93‑1 (MQ=255)

                                        tGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCggg                                            >  2:2615327/1‑93 (MQ=255)

                                        tGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCggg                                            <  1:2352257/93‑1 (MQ=255)

                                         gACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgt                                           >  2:2846195/1‑93 (MQ=255)

                                          aCGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgtg                                          <  2:3011103/93‑1 (MQ=255)

                                          aCGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCG‑ACATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgtg                                          <  1:1112078/93‑1 (MQ=255)

                                             ttGCTTTTTTCTTCCGTCAGCTTATGGTCGCGGAATCTGACGC‑AATCAAATTTAAAACCAATATGTCGCTGGCGATTTCCATCACGGGTGTGc                                       <  2:3555590/92‑1 (MQ=255)

                                             gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGc                                       >  2:3192737/1‑93 (MQ=255)

                                             gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGc                                       <  1:3068977/93‑1 (MQ=255)

                                             gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGc                                       >  2:1909451/1‑93 (MQ=255)

                                              tGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCt                                      <  2:2867467/93‑1 (MQ=255)

                                               gCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGGGTGCTg                                     >  2:1066143/1‑93 (MQ=255)

                                                cTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTgg                                    >  1:4021603/1‑93 (MQ=255)

                                                  ttcttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTa                                  <  2:333927/93‑1 (MQ=255)

                                                  ttcttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTa                                  >  1:144574/1‑93 (MQ=255)

                                                   tcttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTAc                                 <  2:4515992/93‑1 (MQ=255)

                                                     ttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACAt                               >  1:2950079/1‑93 (MQ=255)

                                                     ttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACAt                               >  1:2580564/1‑93 (MQ=255)

                                                      tcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATc                              >  1:2758552/1‑93 (MQ=255)

                                                      tcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATc                              <  2:4579037/93‑1 (MQ=255)

                                                      tcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATc                              <  1:4736435/93‑1 (MQ=255)

                                                        ttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCg                            >  1:1338801/1‑93 (MQ=255)

                                                         tcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGa                           >  2:786631/1‑93 (MQ=255)

                                                         tcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGa                           <  2:2998620/93‑1 (MQ=255)

                                                          ccTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAg                          >  1:2810647/1‑93 (MQ=255)

                                                          ccTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAg                          >  2:481300/1‑93 (MQ=255)

                                                           cTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGt                         <  2:2608248/93‑1 (MQ=255)

                                                            tGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtt                        <  2:1907211/93‑1 (MQ=255)

                                                               cGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTa                     >  1:2880786/1‑93 (MQ=255)

                                                               cGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTa                     >  1:2953477/1‑93 (MQ=255)

                                                                gCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTATATCCGAGTTTTAt                    >  1:3907259/1‑93 (MQ=255)

                                                                  tGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTg                  >  2:4310763/1‑93 (MQ=255)

                                                                  tGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTg                  >  1:3068918/1‑93 (MQ=255)

                                                                   gATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGa                 >  2:1274065/1‑93 (MQ=255)

                                                                   gATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGa                 <  2:4535479/93‑1 (MQ=255)

                                                                   gATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGa                 >  1:2590076/1‑93 (MQ=255)

                                                                    aTGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGaa                <  2:2668541/93‑1 (MQ=255)

                                                                     tGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAAc               <  1:1432658/93‑1 (MQ=255)

                                                                         cgcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGAt           <  2:4207439/89‑1 (MQ=255)

                                                                          gcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGGGATTGCCATCGCGGGTGTGCTGGTCCATCCGAGTTTTATTGAACTGATg          >  1:1543266/4‑93 (MQ=255)

                                                                          gcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATg          >  1:2584111/4‑93 (MQ=255)

                                                                          gcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATg          >  1:38480/4‑93 (MQ=255)

                                                                          gcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATg          <  2:3718216/90‑1 (MQ=255)

                                                                          gcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATg          <  1:3953015/90‑1 (MQ=255)

                                                                           cggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATgg         <  2:1946323/91‑1 (MQ=255)

                                                                                tCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa    <  2:3432316/93‑1 (MQ=255)

                                                                                tCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa    >  1:897188/1‑93 (MQ=255)

                                                                                tCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa    >  2:2216769/1‑93 (MQ=255)

                                                                                 cTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAg   <  1:1739334/93‑1 (MQ=255)

                                                                                 cTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAg   <  1:3651652/93‑1 (MQ=255)

                                                                                  tGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGc  <  1:779588/93‑1 (MQ=255)

                                                                                  tGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGc  >  2:571159/1‑93 (MQ=255)

                                                                                  tGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGc  <  1:2064047/93‑1 (MQ=255)

                                                                                  tGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGGGTGCTGGTACATCCGAGTTTTATTAAACTGATGGCGAAAGc  >  1:1679328/1‑93 (MQ=255)

CAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGC‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGC  >  NC_000913/2837968‑2838142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`