Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,514	Δ1 bp	intergenic (‑165/+149)	cmtB ← / ← tktA	PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,513	0	C	.	100.0%	417.8 / NA	91	intergenic (‑164/+150)	cmtB/tktA	PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (49/42);  total (49/42)

GCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACCGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGC  >  NC_000913/3077421‑3077601                                                                                             |                                                                                         gCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAc                                                                                          <  1:2332163/93‑1 (MQ=255)   aaTTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAcgc                                                                                        >  2:63900/1‑91 (MQ=255)    aTTGCGCAGGGAAACCGGTGTTTTGAAAACAGTGCATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAcgca                                                                                       >  1:2544117/1‑90 (MQ=255)    aTTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAcgca                                                                                       >  1:4173213/1‑90 (MQ=255)    aTTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAcgca                                                                                       >  1:2544116/1‑90 (MQ=255)      tGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCata                                                                                    >  2:1861606/1‑93 (MQ=255)         gCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCg                                                                                 <  2:2661638/93‑1 (MQ=255)          cAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGt                                                                                <  1:53894/93‑1 (MQ=255)          cAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGt                                                                                <  1:53895/93‑1 (MQ=255)           aGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTg                                                                               >  1:1201392/1‑93 (MQ=255)              gAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATg                                                                            <  1:3916845/93‑1 (MQ=255)               aaaCCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGc                                                                           <  2:3090971/93‑1 (MQ=255)               aaaCCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGc                                                                           <  1:800876/93‑1 (MQ=255)                  ccGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCata                                                                        <  2:4332985/93‑1 (MQ=255)                  ccGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCata                                                                        <  1:2038834/93‑1 (MQ=255)                   cGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCatat                                                                       <  1:3376108/93‑1 (MQ=255)                    ggTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATAtt                                                                      >  1:1657621/1‑93 (MQ=255)                     gtgtTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTa                                                                     <  1:3382964/93‑1 (MQ=255)                        ttttGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACAt                                                                  <  2:593963/93‑1 (MQ=255)                           tGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAAt                                                               <  1:91251/93‑1 (MQ=255)                               aaCAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGat                                                           <  1:3891756/93‑1 (MQ=255)                                aCAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGata                                                          <  2:3328172/93‑1 (MQ=255)                                 cAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGatat                                                         <  2:3074475/93‑1 (MQ=255)                                 cAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGatat                                                         <  2:4506353/93‑1 (MQ=255)                                    tGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAtattta                                                      >  2:1319747/1‑93 (MQ=255)                                    tGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAtattta                                                      <  1:4338163/93‑1 (MQ=255)                                     gAATTATTAATTTCTTAAATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAtatttat                                                     >  2:760497/1‑93 (MQ=255)                                      aaTTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAtatttatt                                                    <  1:3675839/93‑1 (MQ=255)                                      aaTTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAtatttatt                                                    <  1:1248871/93‑1 (MQ=255)                                      aaTTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAtatttatt                                                    <  1:210265/93‑1 (MQ=255)                                       attattAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTg                                                   <  1:3886999/93‑1 (MQ=255)                                            taATTTCTTATATAACATTGAGTTATAGACATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCatat                                              <  2:2316724/93‑1 (MQ=255)                                             aaTTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCAtata                                             <  1:260487/93‑1 (MQ=255)                                               tttCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATaaa                                           >  1:4498398/1‑93 (MQ=255)                                               tttCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATaaa                                           <  2:2588754/93‑1 (MQ=255)                                               ttacTTATATATCATTGAGTTATAGATATATCAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATaaa                                           <  1:2516319/90‑1 (MQ=255)                                                ttCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAat                                          <  1:408856/93‑1 (MQ=255)                                                ttCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAat                                          <  1:408855/93‑1 (MQ=255)                                                 tCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAata                                         <  2:4257067/93‑1 (MQ=255)                                                 tCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAata                                         <  2:4257066/93‑1 (MQ=255)                                                 tCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAata                                         >  1:1664370/1‑93 (MQ=255)                                                   ttATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATAtt                                       >  1:3643349/1‑93 (MQ=255)                                                   ttATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATAtt                                       >  2:3601212/1‑93 (MQ=255)                                                   ttATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATAtt                                       >  2:159902/1‑93 (MQ=255)                                                      tataACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtgt                                    <  2:1027453/93‑1 (MQ=255)                                                       ataACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtgtg                                   >  1:222269/1‑93 (MQ=255)                                                       ataACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtgtg                                   >  1:4434882/1‑93 (MQ=255)                                                       ataACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtgtg                                   >  2:3610567/1‑93 (MQ=255)                                                        taACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGa                                  >  2:2005446/1‑93 (MQ=255)                                                         aaCATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGAt                                 <  1:1926970/93‑1 (MQ=255)                                                         aaCATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGAt                                 <  1:4346719/93‑1 (MQ=255)                                                          aCATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATc                                >  1:3270317/1‑93 (MQ=255)                                                          aCATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATc                                >  2:3830006/1‑93 (MQ=255)                                                           cATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCt                               <  1:4716743/93‑1 (MQ=255)                                                           cATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCt                               >  1:1932942/1‑93 (MQ=255)                                                           cATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCt                               >  1:1932941/1‑93 (MQ=255)                                                           cATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCt                               >  2:1157994/1‑93 (MQ=255)                                                            aTTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTa                              >  1:2972970/1‑93 (MQ=255)                                                             ttGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTac                             >  1:2100095/1‑93 (MQ=255)                                                             ttGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTac                             >  2:1787437/1‑93 (MQ=255)                                                               gAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTacaa                           >  1:4451196/1‑93 (MQ=255)                                                               gAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTacaa                           <  1:1309873/93‑1 (MQ=255)                                                                  ttATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAacac                        <  2:4179995/93‑1 (MQ=255)                                                                   tataGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACg                       >  1:4148919/1‑93 (MQ=255)                                                                   tataGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACg                       >  2:4288076/1‑93 (MQ=255)                                                                     taGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGcc                     >  1:303955/1‑93 (MQ=255)                                                                     taGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGcc                     >  2:1839636/1‑93 (MQ=255)                                                                     taGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGcc                     <  1:2685669/93‑1 (MQ=255)                                                                     taGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGcc                     >  2:1150732/1‑93 (MQ=255)                                                                     taGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGcc                     <  1:2685668/93‑1 (MQ=255)                                                                     taGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGcc                     <  1:2685670/93‑1 (MQ=255)                                                                       gATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCtt                   >  1:1617107/1‑93 (MQ=255)                                                                       gATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCtt                   <  1:1561966/93‑1 (MQ=255)                                                                       gATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATCGCATATAAATATTTGTGATCTACAACACGCCtt                   >  1:4572151/1‑93 (MQ=255)                                                                       gATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTAATGCATATAAATATTTGTGATCTACAACACGCCtt                   >  1:2086323/1‑93 (MQ=255)                                                                         tataACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTAt                 >  2:2862684/1‑93 (MQ=255)                                                                          ataACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATc                >  2:1754262/1‑93 (MQ=255)                                                                            aacaacAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTa              <  1:4566305/93‑1 (MQ=255)                                                                             acaacaAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAt             >  2:4350320/1‑93 (MQ=255)                                                                             acaacaAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAt             >  2:528569/1‑93 (MQ=255)                                                                             acaacaAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAt             >  2:2271293/1‑93 (MQ=255)                                                                              caacaaATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAtt            <  1:2346552/93‑1 (MQ=255)                                                                               aacaaATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTg           >  1:2819344/1‑93 (MQ=255)                                                                               aacaaATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTg           >  1:2135542/1‑93 (MQ=255)                                                                                acaaATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGc          >  1:658371/1‑93 (MQ=255)                                                                                 caaATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCt         >  1:466805/1‑93 (MQ=255)                                                                                  aaaTGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCtt        >  1:3563811/1‑93 (MQ=255)                                                                                  aaaTGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCtt        >  2:4281094/1‑93 (MQ=255)                                                                                  aaaTGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCtt        >  2:4115369/1‑93 (MQ=255)                                                                                  aaaTGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCtt        >  1:517631/1‑93 (MQ=255)                                                                                  aaaTGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCtt        >  2:4477089/1‑93 (MQ=255)                                                                                   aaTGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTg       >  1:3538015/1‑93 (MQ=255)                                                                                    aTGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGt      >  1:4426188/1‑93 (MQ=255)                                                                                    aTGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGt      >  2:2921711/1‑93 (MQ=255)                                                                                     tGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTc     <  1:760265/93‑1 (MQ=255)                                                                                      gTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTcc    <  1:4036891/93‑1 (MQ=255)                                                                                        cAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGc  >  2:2106345/1‑93 (MQ=255)                                                                                             |                                                                                         GCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACCGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGC  >  NC_000913/3077421‑3077601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑