| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,957,092 | +CC | intergenic (+26/+58) | ilvC → / ← ppiC | ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,957,091 | 1 | . | C | 98.4% | 159.4 / ‑3.3 | 62 | intergenic (+25/+59) | ilvC/ppiC | ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (3/58); total (3/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
| * | NC_000913 | 3,957,091 | 2 | . | C | 98.4% | 159.7 / ‑3.3 | 62 | intergenic (+25/+59) | ilvC/ppiC | ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (3/58); total (3/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
TTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTCAA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA > NC_000913/3956999‑3957172 || ttGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑a < 2:2140753/93‑1 (MQ=255) tGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑c < 1:2172536/93‑1 (MQ=255) aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑Ccctct < 1:3638144/93‑2 (MQ=255) gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCGCCC‑‑CTCTCCcaca > 1:686502/1‑93 (MQ=37) taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACag < 2:1989207/93‑1 (MQ=37) aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACagg < 2:2791016/93‑1 (MQ=37) aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACagg < 2:637054/93‑1 (MQ=37) gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACaggga < 1:929194/93‑1 (MQ=37) aaaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACaggga < 1:3612524/92‑1 (MQ=37) aaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACagggaga < 2:2463948/93‑1 (MQ=37) aCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACagggagag < 1:2980668/93‑1 (MQ=37) gcgcGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACagggagaggga < 1:1117799/93‑1 (MQ=25) gcgcGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACagggagaggga < 2:4466141/93‑1 (MQ=25) gcgcGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACagggagaggga < 2:4537271/93‑1 (MQ=25) ggCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGaaa < 2:2479171/93‑1 (MQ=25) gCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGaaaa < 1:3318265/93‑1 (MQ=21) gCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGaaaa < 1:3318266/93‑1 (MQ=21) gCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGaaaa < 1:3726013/93‑1 (MQ=21) gCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGaaaa < 1:4106262/93‑1 (MQ=21) cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAac < 2:342729/93‑1 (MQ=21) cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAac < 1:1496496/93‑1 (MQ=21) tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCCCAGGGAGGGGGGGAAAaca > 2:4731513/1‑93 (MQ=21) tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAaca < 1:3315249/93‑1 (MQ=21) tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAAGGAGAGGGAGaaaaaa > 2:796281/1‑91 (MQ=21) atatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAacac < 1:3560124/93‑1 (MQ=21) atatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAacac < 2:542502/93‑1 (MQ=21) tatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCC‑‑ACCCCCTCCCACAGGGAGAGGGGGaaaaacac > 2:4695305/1‑89 (MQ=21) tGACAGATATGAAACTTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCa < 1:320225/93‑1 (MQ=21) gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCaa < 1:1814825/93‑1 (MQ=21) aaaGATATGAATGTTATTGCTGTTGCTGGTTAAGTGCGCGCTGAGGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAA‑g < 1:1324441/91‑1 (MQ=17) aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAA‑g < 2:2121640/93‑1 (MQ=21) cAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAA‑gg < 2:2757683/93‑2 (MQ=21) aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACCCAA‑ggc < 2:3588179/93‑3 (MQ=21) gATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAA‑ggcc < 1:2876473/93‑4 (MQ=21) gATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAA‑ggcc < 1:3281217/93‑4 (MQ=21) atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAA‑ggcct < 1:98335/93‑5 (MQ=21) atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtc < 2:1026483/93‑1 (MQ=18) gAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtctc < 1:1789410/93‑1 (MQ=18) aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTcc < 1:2337875/93‑1 (MQ=18) aCGTATTGCGGTGGGGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg < 2:1217104/93‑1 (MQ=17) cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTgg < 2:3637162/93‑1 (MQ=18) gTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga < 1:461609/93‑1 (MQ=21) gTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCCCACCCCG‑‑ACCCTCTCCCACACGGGGAGGGGGAAAAAACTCAAGGACTTCTTCTGga > 2:3231444/1‑93 (MQ=12) gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAgg < 2:1006014/93‑1 (MQ=18) gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAgg < 2:2113099/93‑1 (MQ=18) gCTGTTGCGCGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAgg < 2:645493/93‑1 (MQ=18) tGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGcc < 2:3372444/93‑1 (MQ=18) gTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt < 2:1675889/93‑1 (MQ=18) ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGATAGGTAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCtt < 1:1619048/93‑1 (MQ=18) tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTg < 1:1652904/93‑1 (MQ=18) gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGc < 2:2292213/93‑1 (MQ=18) gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGc < 2:649743/93‑1 (MQ=18) gggTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTa < 1:494222/93‑1 (MQ=18) gTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAtt < 1:2546902/93‑1 (MQ=18) ttAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa < 2:111654/93‑1 (MQ=18) aaGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGt < 1:581871/93‑1 (MQ=18) aaGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGt < 1:4531956/93‑1 (MQ=18) gcgcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg < 1:1301489/93‑1 (MQ=21) gcgcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg < 1:4549574/93‑1 (MQ=21) cgcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg < 1:1987246/93‑1 (MQ=21) cgcgcTGATGCCCGCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg < 1:1987245/93‑1 (MQ=21) gcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGt < 2:4723522/93‑1 (MQ=21) cgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTa < 1:4013827/93‑1 (MQ=21) tGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAc < 2:813318/93‑1 (MQ=25) gCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAcc < 1:980248/93‑1 (MQ=25) cccTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCt < 2:3024969/93‑1 (MQ=25) ccTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCtt > 1:1652386/1‑93 (MQ=37) tCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGGGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa > 2:3754238/1‑93 (MQ=37) tCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa < 1:444171/93‑1 (MQ=37) || TTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTCAA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA > NC_000913/3956999‑3957172 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |