BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,980,297	(C)_8→9	noncoding (79/87 nt)	leuT →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,980,289	1	.	C	84.6%	237.5 / 42.1	91	noncoding (71/87 nt)	leuT	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (4/10); new base C (22/55); total (26/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAC  >  NC_000913/3980199‑3980376

ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑cc                                                                                         <  2:79607/93‑1 (MQ=255)

ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑cc                                                                                         >  2:4061708/1‑93 (MQ=255)

ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑cc                                                                                         <  1:3881517/93‑1 (MQ=255)

  gttaGAAGTGGTGACAAGGCGAAGGTCGCGGAATTGGTAGACGCGCGAGCTGCCGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑cccc                                                                                       <  1:3233895/92‑1 (MQ=14)

  attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑cccc                                                                                       <  1:3233894/93‑1 (MQ=255)

  attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑G‑T‑‑cccc                                                                                       >  2:4144341/1‑93 (MQ=255)

     aGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑cccc‑ccc                                                                                   <  1:4443699/93‑1 (MQ=255)

       aaGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑cccc‑ccc‑cc                                                                                >  1:2290432/1‑92 (MQ=255)

        aGTTGTTACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑cccc‑ccc‑cct                                                                               <  1:3646834/93‑3 (MQ=255)

        aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑cccc‑ccc‑cct                                                                               <  1:2088397/93‑3 (MQ=255)

        aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑cccc‑ccc‑cct                                                                               <  2:3517595/93‑3 (MQ=255)

        aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑cccc‑ccc‑cct                                                                               <  1:288918/93‑3 (MQ=255)

               ccAATGCCAAGGTGGCGGAATTGGTAGACCCGCTAGCTTCAGGTGTTAGTGTCCTTACGGCCGTGGGGGTGCAA‑G‑TC‑CCCC‑CCC‑CTCGcacca                                                                         <  2:1344110/92‑1 (MQ=11)

               aCAATGCGAAGGTGGCGGAATTGGGAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTTCGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGcacca                                                                         <  2:387615/93‑1 (MQ=11)

                   tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCCCC‑CCC‑CTCGCACCACGa                                                                      >  1:4714956/1‑93 (MQ=11)

                   tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGAc                                                                     <  1:662669/93‑1 (MQ=255)

                   tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGAc                                                                     >  1:2447956/1‑93 (MQ=255)

                   tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGAc                                                                     >  1:2447957/1‑93 (MQ=255)

                     cGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑GCC‑‑CCCC‑CCC‑CTCGCACCACGACtt                                                                   <  2:3436430/93‑1 (MQ=255)

                     cGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACtt                                                                   <  1:4372665/93‑1 (MQ=255)

                     cGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACtt                                                                   >  2:1247587/1‑93 (MQ=255)

                     cGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACtt                                                                   <  1:3760428/93‑1 (MQ=255)

                     cGAAGGGTGCGGAATTGGGAGACCCGCTAACTTCAGGTGTTAGGTTCCTTACGGGCGGGGGGGTTTAA‑A‑TCCCCCC‑CCC‑CTCGCACCACGACt                                                                    <  2:2204694/93‑1 (MQ=2)

                      gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACttt                                                                  >  2:673528/1‑93 (MQ=255)

                      gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACttt                                                                  <  1:4490339/93‑1 (MQ=255)

                      gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑CCCC‑CCC‑CTCGCACCACGACTTTa                                                                 <  2:1544884/93‑1 (MQ=39)

                        aGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTaa                                                                <  1:3871886/93‑1 (MQ=255)

                         ggTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCCCGGCTTTaaa                                                               >  2:4434767/1‑93 (MQ=11)

                          gTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGa                                                             <  2:1647837/93‑1 (MQ=255)

                          gTGGCAGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGGGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGa                                                             <  2:1647838/93‑1 (MQ=255)

                           tGGCGGAATTGGTAGACGCCCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGa                                                             <  1:1010075/93‑1 (MQ=255)

                            ggCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAAt                                                           >  1:3794738/1‑93 (MQ=255)

                             gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAAt                                                           <  1:852080/93‑1 (MQ=255)

                             gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAAt                                                           <  1:852081/93‑1 (MQ=255)

                              cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAAtt                                                          <  1:2674441/93‑1 (MQ=255)

                              cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAAtt                                                          >  2:1204543/1‑93 (MQ=255)

                                 aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGaa                                                       <  2:4118785/93‑1 (MQ=255)

                                 aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGaa                                                       <  2:2950925/93‑1 (MQ=255)

                                 aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAAAATTGaa                                                       >  1:1114948/1‑93 (MQ=255)

                                  aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCCCCACGACTTTAAAGAATTGAAc                                                      <  1:4599538/93‑1 (MQ=255)

                                  aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAAc                                                      <  2:2553234/93‑1 (MQ=255)

                                  aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAAAATTTaaa                                                      >  1:1850335/1‑92 (MQ=14)

                                   ttGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTa                                                    >  1:3002266/1‑93 (MQ=255)

                                    tGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTa                                                    <  2:395209/93‑1 (MQ=255)

                                     ggTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTaa                                                   <  2:490745/93‑1 (MQ=255)

                                       tAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCCCCACGACTTTAAAGAATTGAACTaaaa                                                 >  1:732062/1‑93 (MQ=255)

                                        aGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTaaaaa                                                <  2:1575176/93‑1 (MQ=255)

                                        aGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTaaaaa                                                <  1:2623618/93‑1 (MQ=255)

                                        aGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTaaaaa                                                <  1:3234551/93‑1 (MQ=255)

                                         gACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAAt                                               <  1:891443/93‑1 (MQ=255)

                                         gACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑CCCCACCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAAt                                               <  1:1223861/93‑1 (MQ=255)

                                          gcgcgCTAGGTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAAtt                                              <  1:3401256/92‑1 (MQ=18)

                                            gcgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCa                                            >  1:1465626/1‑93 (MQ=255)

                                               gtAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaa                                         <  2:1154183/92‑1 (MQ=255)

                                               cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaa                                         <  2:721850/93‑1 (MQ=255)

                                               cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                        >  2:3694781/1‑93 (MQ=255)

                                                tggCTTCAGGTGTTAGTGTCCTTACCGACGTGGGGCTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                        <  2:4001929/91‑1 (MQ=21)

                                                tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                        >  2:2419349/1‑93 (MQ=255)

                                                  gCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGCACTAAAAATTCAAAAAGCa                                     >  2:2468957/1‑93 (MQ=255)

                                                   cTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCa                                     <  2:1355409/93‑1 (MQ=255)

                                                   cTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCa                                     <  2:1295553/93‑1 (MQ=255)

                                                   cTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCa                                     <  1:1408734/93‑1 (MQ=255)

                                                   cTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCa                                     <  1:4132202/93‑1 (MQ=255)

                                                   cTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCa                                     <  2:4109811/93‑1 (MQ=255)

                                                     tCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGt                                   <  2:4166206/93‑1 (MQ=255)

                                                     tCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                  <  2:4009905/93‑1 (MQ=255)

                                                     tCAGGTCTTAGTGTCCTTACGGACGTGAGGGTTGAA‑G‑G‑‑CCCC‑CCT‑CTCGCCCCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                  <  1:97881/93‑1 (MQ=25)

                                                      cAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                  <  1:2127230/93‑1 (MQ=255)

                                                      cAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                  <  1:4030981/93‑1 (MQ=255)

                                                       aGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCCCC‑CCC‑CTCGCCCCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                  >  1:4525628/1‑93 (MQ=25)

                                                       aGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAt                                 <  1:3363719/93‑1 (MQ=255)

                                                        ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑GCC‑‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                <  1:3964533/93‑1 (MQ=255)

                                                        ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                <  1:3964532/93‑1 (MQ=255)

                                                          tgtTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                              <  2:516682/93‑1 (MQ=255)

                                                          tgtTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                              <  1:1635518/93‑1 (MQ=255)

                                                           gtTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                             <  1:409392/93‑1 (MQ=255)

                                                                tgtCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAg                        <  2:262866/93‑1 (MQ=255)

                                                                tgtCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAg                        <  2:4054702/93‑1 (MQ=255)

                                                                 gtCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTAAAAAAGCAGAATTTCGGCGAGt                       >  1:1604630/1‑93 (MQ=37)

                                                                  tCCTTACGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                      <  2:597193/93‑1 (MQ=255)

                                                                       gcGGACGTGGGGGTTAAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCa                 <  1:2756236/92‑1 (MQ=39)

                                                                       aCGGACGTGGGGGTTCAA‑GCC‑‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCa                 <  2:1511225/93‑1 (MQ=255)

                                                                       aCGGACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCa                 <  2:2498781/93‑1 (MQ=255)

                                                                          gACGTGGGGGTTCAAGG‑C‑‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCt              <  2:3681178/93‑1 (MQ=255)

                                                                          gACGTGGGGGTTCAA‑G‑TCCCCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGc               <  1:3255808/93‑1 (MQ=39)

                                                                          gACGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCt              >  2:2785528/1‑93 (MQ=255)

                                                                           aCGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCtt             <  1:3821955/93‑1 (MQ=255)

                                                                           aCGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCtt             <  1:3291849/93‑1 (MQ=255)

                                                                           aCGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCtt             >  2:3107675/1‑93 (MQ=255)

                                                                            cGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTg            <  1:4007226/93‑1 (MQ=255)

                                                                            cGTGGGGGTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTg            <  1:3740265/93‑1 (MQ=255)

                                                                               gggggTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGtg         >  1:1072529/1‑92 (MQ=255)

                                                                                ggggTTCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg        <  2:3318583/93‑1 (MQ=255)

                                                                                    ttCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTTGGCGAGTAGCGCAGCTTGGTAGCGCa    >  2:882805/1‑93 (MQ=255)

                                                                                    ttCAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCa    <  1:703450/93‑1 (MQ=255)

                                                                                     tCAA‑G‑T‑‑CCCC‑CCCGCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCaa   <  2:667917/93‑1 (MQ=255)

                                                                                      cAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAc  >  2:3584630/1‑93 (MQ=255)

                                                                                      cAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAc  <  2:2219027/93‑1 (MQ=255)

                                                                                      cAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAc  <  2:2219026/93‑1 (MQ=255)

                                                                                      cAA‑G‑TC‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAc  <  1:1053254/93‑1 (MQ=255)

TTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑‑CCCC‑CCC‑CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAC  >  NC_000913/3980199‑3980376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`