BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,005,601	+C	coding (237/282 nt)	b4568 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,005,600	1	.	C	99.4%	569.9 / ‑5.7	176	G79A (GGC→GCC)	b4568	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (108/66); minor base T (1/0); total (110/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 186 total aligned reads displayed.

GTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGG‑CTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTG  >  NC_000913/4005509‑4005687

gTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGG‑c                                                                                        >  2:165865/1‑93 (MQ=255)

 tAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGGGGTGG‑cc                                                                                       >  2:53619/1‑92 (MQ=255)

 tAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGG‑cc                                                                                       >  1:162333/1‑92 (MQ=255)

   aTGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGG‑cctg                                                                                     <  1:425889/93‑4 (MQ=255)

      gTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTAt                                                                                   <  1:4098144/93‑1 (MQ=255)

       tttGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTAtc                                                                                  >  2:58889/1‑93 (MQ=255)

         tGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTAtctc                                                                                <  1:1078962/93‑1 (MQ=255)

           ggCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTctg                                                                              >  1:4188951/1‑93 (MQ=255)

              tGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTctgctg                                                                           <  2:2121319/93‑1 (MQ=255)

              tGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTctgctg                                                                           >  2:193524/1‑93 (MQ=255)

                ggATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGa                                                                         <  2:1143206/93‑1 (MQ=255)

                ggATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGa                                                                         <  1:3155732/93‑1 (MQ=255)

                  aTTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATg                                                                       >  2:751242/1‑93 (MQ=255)

                   ttGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATgg                                                                      <  2:3523949/93‑1 (MQ=255)

                   ttGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATgg                                                                      <  2:1830444/93‑1 (MQ=255)

                    tGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATggg                                                                     <  2:2727837/93‑1 (MQ=255)

                     gcgcTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGt                                                                    >  2:1613477/1‑93 (MQ=255)

                      cgcTGCTTGGCCTGCATTTGATTAGCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGtt                                                                   >  2:4554886/1‑93 (MQ=255)

                       gctgctTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTa                                                                  >  2:667416/1‑93 (MQ=255)

                       gctgctTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTa                                                                  >  2:1259057/1‑93 (MQ=255)

                       gctgctTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTa                                                                  >  2:512849/1‑93 (MQ=255)

                        ctgctTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTAc                                                                 <  2:621006/93‑1 (MQ=255)

                        ctgctTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTAc                                                                 <  1:1989037/93‑1 (MQ=255)

                         tgctTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTAcc                                                                >  1:1420884/1‑93 (MQ=255)

                          gctTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCa                                                               >  1:2574192/1‑93 (MQ=255)

                           ctTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGGTGGGTTACCAg                                                              >  2:546011/1‑93 (MQ=255)

                           ctTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAg                                                              >  1:2799483/1‑93 (MQ=255)

                             tGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGAt                                                            <  1:2184877/93‑1 (MQ=255)

                               gCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGc                                                          >  2:494253/1‑93 (MQ=255)

                                  tGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTTTGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTAc                                                       >  1:3537649/1‑93 (MQ=255)

                                  tGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTCCCAGATGCTAc                                                       <  1:3147878/93‑1 (MQ=255)

                                  tGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTAc                                                       >  1:454740/1‑93 (MQ=255)

                                  tGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTAc                                                       >  2:3013170/1‑93 (MQ=255)

                                  tGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTAc                                                       >  1:3655297/1‑93 (MQ=255)

                                   gCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACg                                                      >  1:2957423/1‑93 (MQ=255)

                                   gCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACg                                                      >  2:1139341/1‑93 (MQ=255)

                                   gCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGATACCAGATGCTACg                                                      >  2:1663158/1‑93 (MQ=255)

                                    cATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGATACCAGCTGCTACgc                                                     >  2:2438130/1‑92 (MQ=255)

                                    cATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGACTGTATCTCTGCTGGATGGGTTACCAGATGCTACgg                                                     >  2:4408939/1‑92 (MQ=255)

                                     aTTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACgtg                                                    <  2:1416363/93‑1 (MQ=255)

                                     aTTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACgtg                                                    >  2:2902128/1‑93 (MQ=255)

                                      tttGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTGCCAGATGCTACgtgg                                                   <  2:3079024/93‑1 (MQ=255)

                                       ttGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACgtggt                                                  <  1:71936/93‑1 (MQ=255)

                                        tGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACgtggtg                                                 >  2:3290921/1‑93 (MQ=255)

                                           ttATCGAAAAAATTGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCGCTGCTGGAGGGGTAGGCGGATGCTACGTGGGGCAc                                              >  1:3269547/1‑93 (MQ=255)

                                           ttATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCAc                                              >  1:4390561/1‑93 (MQ=255)

                                           ttATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCAc                                              >  1:1938715/1‑93 (MQ=255)

                                            tATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGTCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACt                                             >  2:2802771/1‑93 (MQ=255)

                                            tATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACt                                             >  2:2538717/1‑93 (MQ=255)

                                            tATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACt                                             <  2:228096/93‑1 (MQ=255)

                                                gAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGaaa                                         >  1:3647119/1‑93 (MQ=255)

                                                 aaaaaaTGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGaaaa                                        <  1:4462795/93‑1 (MQ=255)

                                                  aaaaaTGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGaaaaa                                       <  1:3094202/93‑1 (MQ=255)

                                                   aaaaTGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGaaaaaa                                      <  1:1487113/93‑1 (MQ=255)

                                                   aaaaTGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGaaaaaa                                      <  1:2141149/93‑1 (MQ=255)

                                                    aaaTGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAag                                     >  1:1049425/1‑93 (MQ=255)

                                                    aaaTGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAag                                     >  2:3863433/1‑93 (MQ=255)

                                                     aaTGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAaga                                    <  2:731649/93‑1 (MQ=255)

                                                     aaTGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAaga                                    <  2:3280308/93‑1 (MQ=255)

                                                       tGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAgg                                  <  1:1550284/93‑1 (MQ=255)

                                                       tGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAgg                                  <  1:3921708/93‑1 (MQ=255)

                                                        ggCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGc                                 <  2:3727757/93‑1 (MQ=255)

                                                        ggCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGc                                 >  2:3655162/1‑93 (MQ=255)

                                                        ggCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGc                                 <  1:3367613/93‑1 (MQ=255)

                                                         gCCTGGCTGCATGCGCTGATTATGGTGGGCGGTGGCCTGGATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCg                                <  2:1293591/93‑1 (MQ=255)

                                                         gCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCg                                <  1:4337461/93‑1 (MQ=255)

                                                         gCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCg                                >  1:3031109/1‑93 (MQ=255)

                                                         gCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCg                                <  2:1051623/93‑1 (MQ=255)

                                                           cTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGt                              >  1:2076030/1‑93 (MQ=255)

                                                            tGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGtt                             >  1:1791522/1‑93 (MQ=255)

                                                            tGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGtt                             >  2:2062127/1‑93 (MQ=255)

                                                             ggCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGttt                            <  1:3867859/93‑1 (MQ=255)

                                                              gCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTc                           >  1:1441162/1‑93 (MQ=255)

                                                               cTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCt                          >  1:2052449/1‑93 (MQ=255)

                                                               cTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCt                          >  1:4629928/1‑93 (MQ=255)

                                                                tGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGCACAAAGAGGCGGTTTCTg                         >  1:3137795/1‑93 (MQ=255)

                                                                tGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTg                         >  1:4363104/1‑93 (MQ=255)

                                                                tGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTg                         <  2:553920/93‑1 (MQ=255)

                                                                tGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTg                         >  1:424217/1‑93 (MQ=255)

                                                                 gCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGc                        >  1:3314423/1‑93 (MQ=255)

                                                                    tACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATTCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCAcc                     <  2:2395160/93‑1 (MQ=255)

                                                                    tACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCAcc                     >  2:31171/1‑93 (MQ=255)

                                                                    tACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCAcc                     >  2:4740700/1‑93 (MQ=255)

                                                                       gCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCtac                  >  1:2906757/1‑91 (MQ=255)

                                                                       gCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTgc                  >  1:122824/1‑93 (MQ=255)

                                                                        cTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGTAAAAAGAGGCGGTTTCTGCACCTgcg                 >  1:13196/1‑93 (MQ=255)

                                                                        cTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTgcg                 >  2:3826309/1‑93 (MQ=255)

                                                                        cTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGAACCTgcc                 >  1:2662960/1‑92 (MQ=255)

                                                                          gATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGcc               >  2:3108067/1‑93 (MQ=255)

                                                                            ttATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCcaa             >  2:2471231/1‑92 (MQ=255)

                                                                                ggtggGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGt         <  2:4234166/93‑1 (MQ=255)

                                                                                ggtggGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCAGTTTCTGCACCTGCGCCACAGGt         >  2:3395090/1‑93 (MQ=255)

                                                                                 gtggGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTc        >  2:3431179/1‑93 (MQ=255)

                                                                                 gtggGCGGTGGCCTGTATCTCTGCTGGATGGGTTACAAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTc        <  1:269414/93‑1 (MQ=255)

                                                                                    ggCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAg     >  1:233758/1‑93 (MQ=255)

                                                                                     gCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGc    <  2:4234259/93‑1 (MQ=255)

                                                                                     gCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGc    <  2:40170/93‑1 (MQ=255)

                                                                                     gCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGc    <  2:1388593/93‑1 (MQ=255)

                                                                                      cGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCt   >  1:9043/1‑93 (MQ=255)

                                                                                       ggTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTg  <  1:3768693/93‑1 (MQ=255)

GTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGG‑CTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTG  >  NC_000913/4005509‑4005687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`